The importance of service science is emphasized in the modern economy, and the value and necessity of service research still increasing. Since the service research framework was proposed, it has been studied from various perspectives and incorporated into one framework--service research. The direction of service research has been established and a new baseline of research has been established. However, the modern economic and social environment could be described as a new era, the Fourth Industrial Revolution has changed drastically. More and more systematic research on services has become necessary. Therefore, this study analyzed the field of service research in the existing framework. The study suggested how service research could broaden the horizon of service research by studying the 'what'. To do this, we analyzed recent service research trends by themes. We also identified the shortcomings of previous studies about service, and suggested directions and research themes for future research. Based on this study we developed a general approach to the creation of new models from the viewpoint of service science. The authors were also able to develop a general approach to areas such as service innovation, service inference, service solution, and service design leverage. In addition, it is necessary to extend service research and business model to the utilization of service technology. This approach could contribute to forming the basis of future service development, and to utilize social media to create new value of innovative company. The results of this study could contribute to deepening and expanding service research.
Lee, Hong;Shin, Chang Hoon;Kim, Hye Ree;Choi, Kyung Hee;Kim, Hyeon Ho
Molecules and Cells
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v.40
no.4
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pp.254-261
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2017
Glioblastomas (GBM) are very difficult to treat and their aggressiveness is one of the main reasons for this as well as for the frequent recurrences. MicroRNAs post-transcriptionally regulate their target genes through interaction between their seed sequence and 3'UTR of the target mRNAs. We previously reported that miR-296-3p is regulated by neurofibromatosis 2 (NF2) and enhances the invasiveness of GBM cells via SOCS2/STAT3. In this study, we investigated whether miR-296-5p, which originates from the same precursor miRNA as miR-296-3p, can increase the invasiveness of GBM cells. It was observed that miR-296-5p potentiated the invasion of various GBM cells including LN229, T98G, and U87MG. Through bioinformatics approaches, two genes were identified as miR-296-5p targets: caspase-8 (CASP8) and nerve growth factor receptor (NGFR). From results obtained from Ago2 immunoprecipitation and luciferase assays, we found that miR-296-5p downregulates CASP8 and NGFR through direct interaction between seed sequence of the miRNA and 3'UTR of the target mRNA. Knockdown of CASP8 or NGFR also increased the invasive ability of GBM cells, indicating that CASP8 and NGFR are involved in potentiation of invasiveness by miR-296-5p. Consistent with our findings, CASP8 was downregulated in brain metastatic lung cancer cells, which have a high level of miR-296-5p, compared to parental cells, suggesting that miR-296-5p may be generally associated with the acquisition of invasiveness. Collectively, our results implicate miR-296-5p as a potential cause of invasiveness in cancer and suggest it as a promising therapeutic target for GBM.
Kim, Young Hoon;Park, Eun Gyu;Batsuren, Dulamjav;Ganbaatar, Jamsranjav;Nho, Chu Won;Pan, Cheol-Ho;Lee, Jae Kwon
Journal of Applied Biological Chemistry
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v.57
no.4
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pp.373-377
/
2014
In this study, we demonstrated whether the extract of Fomes fomentarius (FFE; FF extract) could be used to stimulate macrophages (RAW 264.7 cells). All four doses of FFE (5, 10, 20, and $40{\mu}g/mL$) had no significant cytotoxicity during the entire experimental period. FFE potently increased the production of nitric oxide (NO). Consistent with these observations, inducible NO synthase levels were increased by FFE in a dose-dependent manner. Moreover, FFE increased the production of tumor necrosis factor-${\alpha}$, interleukin (IL)-$1{\beta}$, and IL-6 in the same cells. These stimulating effects of FFE were found to be caused by the stimulation of phosphorylation of $I{\kappa}B{\alpha}$ and MAP kinases (p38, ERK, and JNK). These results suggest that FFE may be used as new agents for wide application in the immune study of mushroom.
The residence space has been in the trend of being expanded with the J'l'cent economic growth Therefore PAC may be said to have [wen from business use to home US(: the progressed living standard as the goods ior Uw "ummel' S('dSOn rest'ntly though sold in Uw fast for mainly buslTw"s use. PAC has been in the demand day ;lner day and in the tn'nd on the bc,ing the necessJty of the n'sent wf',lther warming phenomenon This study is the case study nf the products development and design process of PAC which has bpcn llrivcn and developed by Gold Star Co. ,Ltd. Which .is the firm for home electric appliances representing Korea. This studies for the development and the design of the products by the firm thus emphasizing the visulizing it based on this study. For example as they being the goods for the summer season I have tried to know the physical quakity and the characters of koreans by the weather conditions and estimated the demand by setting up the target customers of the PAC as the customers' environment. And I analyzed the acts in the living room in the apartments as the living envinorment which has the direct relation with PAC and also became a help to the moulding work of PAC by analyzing the complete interior image in the living room. In the practical moulding work the Design concept was classified into the envinorment of customer, living and products to drive as the below. Firstly - New shape (Round slim) has been realized which leads the minimizing the moulding space and visul sense of opening as its being spacious. The 2nd - the high class sense of the products which are harmonious with the atmosphere of the living room by making it interiorized has been visible. The 3rd - The shape stimulating the sensitivity of the users and surface treatment are emphasized by making it be touched. The 4th - During inviting use of technology image which appears in the electronics products are attemptedby making it be high quality.king it be high quality.
Koreanishche Zeitschrift fur Deutsche Sprachwissenschaft
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v.9
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pp.195-215
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2004
In der vorliegenden Arbeit wird die $M\"{o}glichkeit$ der Unterklassifikation des Sprechakttyps untersucht. Zur Klassifikation von Sprechakten hat Searle (1969) $zw\"{o}lf$ Kriterien vorgeschlagen, und mit den folgenden drei Kriterien hat er funf Sprechaktklassen herausgearbeitet: $illokution\"{a}rer$ Zweck, Entsprechungsrichtung und psychologischer Zustand. Bei der Unterklassifikation dieser $f\"{u}nf$ Sprechaktklassen $k\"{o}nnen$ aber die von Searle vorgeschlagenen Klassifikationskriterien kaum gebraucht werden, so dass zur Unterklassifikation jeder Sprechaktklasse neue Kriterien $eingef\"{u}hrt$ werden mussten. Dabei stellt sich die Frage, was als Unterklassifikationskriterien dienen und wie ausdifferenziert eine Spreckaktklasse bzw. ein Sprechakttyp unterklassifiziert werden kann. Im 1. Kapitel werden neben den Klassifikationskriterien von Searle die Kriterien, die in den bisherigen Unterklassifikationen der Sprechaktklassen verwendet wurden, kritisch betrachtet. Im 2. Kapitel wird auf die Frage der Ausdifferenzierungsgrenze $n\"{a}her$ eingegangen, wobei diskutiert wird, ob die Unterklassifikation der Fragehandlung von Hindelang (1981) und die des Insistierens von Franke (1990) genugend ausdifferenziert sind oder es noch $M\"{o}glichkeiten$ besteht, sie noch weiter auszudifferenzieren. Die Frage nach der Ausdifferenzierungsgrenze ist mit der Frage gleichzusetzen, inwieweit eine Sprechhandlung ohne Verlust ihres Handlungscharakters in Untertypen zerlegt werden kann. Um diese Frage zu beantworten, wird der Begriff 'Basishandlung' $eingef\"{u}hrt$, den von Wright (1974) im Rahmen seiner Theorie der Kausalitat entwickelt hat. In Analogie zu diesem Begriff wird der Begriff 'Basis-Sprechhandlung' vorgeschlagen und wie folgt definiert: 1. Obere Grenze: Eine Basis-Sprechhandlung ist die Sprechhandlung, die nicht durch den Vollzug der anderen Sprechhandlungen ausgefuhrt werden kann. 2. Untere Grenze: Durch eine $\"{A}u{\ss}erung$ bzw. einen Teil der $\"{A}{\ss}erung$ kann nur dann eine Basis-Sprechhandlung vollzogen werden, wenn sieler eine $\"{A}nderung$ des Wissens oder der Handlung des $H\"{o}rers$$herbeif\"{u}hrt$. Diese These wird anhand der Beispiele $\"{u}berpr\"{u}ft$, deren kommunikative $Selbst\"{a}ndigkeit$ schon durch andere Studien $best\"{a}tigt$ wurde. Als weiteres Kriterium zur Entscheidung $\"{u}ber$ die $M\"{o}glichkeit$ der Unterklassifikation wird im 4. Kapitel der Begriff 'kommunikativer Zweck' $eingef\"{u}hrt$ und $dar\"{u}ber$ diskutiert, wie der kommunikative Zweck der Untertypen einer Sprechaktklasse bzw. eines Sprechakttyps definiert und beschrieben werden kann. Dabei wird festgestellt, dass er durch die Spezifizierung des kommunikativen Zwecks der Sprechaktklasse bzw. des Sprechakttyps, zu der/zu dem die Untertypen $geh\"{o}ren$, definiert und beschrieben werden kann, wobei die semantischen, die kontextuellen und die sitiativen Aspekte $mitbe\"{u}cksichtigt$ werden sollen.
Proceedings of the Korean Society of Developmental Biology Conference
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2003.10a
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pp.101-101
/
2003
Main strategy for a treatment of Parkinson's disease (PD), due to a progressive degeneration of dopaminergic neurons, is a pharmaceutical supplement of dopamine derivatives or ceil replacement therapy. Both of these protocols have pros and cons; former exhibiting a dramatic relief but causing a severe side effects on long-term prescription and latter also having a proven effectiveness but having availability and ethical problems Embryonic stem (ES) cells have several characteristics suitable for this purpose. To investigate a possibility of using ES cells as a carrier of therapeutic gene(s), human ES (hES, MB03) cells were transfected with cDNAs coding for tyrosine hydroxylase (TH) in pcDNA3.1 (+) and the transfectants were selected using neomycin (250 $\mu /ml$). Expression of TH being confirmed, two of the positive clone (MBTH2 & 8) were second transfected with GTP cyclohydrolase 1 (GTPCH 1) in pcDNA3.1 (+)-hyg followed by selection with hygromycin-B (150 $\mu /ml$) and RT-PCR confirmation. By immune-cytochemistry, these genetically modified but undifferentiated dual drug-resistant cells were found to express few of the neuronal markers, such as NF200, $\beta$-tubulin, and MAP2 as well as astroglial marker GFAP. This results suggest that over-production of BH4 by ectopically expressed GTPCH I may be involved in the induction of those markers. Transplantation of the cells into striatum of 6-OHDA- denervated PD animal model relieved symptomatic rotational behaviors of the animals. Immunohistochemical analyses showed the presence of human cells within the striatum of the recipients. These results suggest a possibility of using hES cells as a carrier of therapeutic gene(s).
Min Kyung-Yoon;Kim, Chang-Guhn;Kim, Hyun-Jeong;Lim, Hyung-Guhn;Rho, Ji-Young;Juhng Seon-Kwan;Won Jong-Jin;Yang, David J.
The Korean Journal of Nuclear Medicine
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v.30
no.3
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pp.344-350
/
1996
Purpose : Noninvasive imaging of tumor cell proliferation could be helpful in the evaluation of tumor growth potential and could provide an early assessment of treatment response. Radiolabeled thymidine, uridine and adenosine have been used to evaluate tumor cell proliferation. These nucleoside analogs are incorporated into DNA during proliferation. Iodine-131-Iodomethyluridine, an analog of Iodine-131-Iododeoxyuridine, is also involved in DNA/RNA synthesis. The purpose of this study was to develop Iodine-131-Iodomethylurdine and image tumor proliferation using Iodine-131-Iodomethyluridine. Materials and Methods : Radiosynthesis of Iodine-131-5-Iodo-2'-O-methyluridine (Iodine-131-Iodomethyluridine) was prepared from 10 mg of 2'-O-methyluridine(Sigma chemical Co., St. Louis, Missouri) and 2.1 mCi(SP. 10Ci/mg) of Iodine-131-labeled sodium iodide in $100{\mu}l$ of water using iodogen reaction. Female Fischer 344 rats were inoculated in the thigh area with breast tumor cells(13765 NF, $10^5$ cells/rat S.C.). After 14 days, the Iodine-131-Iodomethyluridine $10{\mu}Ci$ was injected to three groups of rats(3/group). The percent of injected dose per gram of tissue weight was determined at 0.5-hours, 2-hours, 4-hours, and 24-hours respectively. Tumor bearing rats after receiving Iodine-131-Iodomethyluridine($50{\mu}Ci$ IV) were euthanized at 2 hours after injection. Autoradiography was done using freeze-dried $50{\mu}m$ coronal section. After injection of Iodine-131- Iodomethyluridine ($10{\mu}Ci$/rat, IV) in three breast tumor-bearing rats, planar scintigraphy was taken at 45 minutes, 90 minutes and 24 hours. Results : Iodine-131-Iodomethyluridine was conveniently synthesized using iodogen reaction. The biodistribution showed fast blood clearance and the tumor-to-tissue uptake ratios showed that optimal imaging time was at 2 hours postinjection. Autoradiogram and planar scintigram indicated that tumor could be well visualized. Conclusion : The findings suggest that Iodine-131-Iodomethyluridine, a new radio-iodinated nucleoside, has potential use for evaluation of active regions of tumor growth.
Baek, Jong Min;Kim, Ju Young;Lee, Myeung Su;Jeung, Woo Jin;Moon, Seo Young;Jeon, Byung Hoon;Oh, Jae Min;Choi, Min Kyu
Journal of Physiology & Pathology in Korean Medicine
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v.27
no.4
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pp.431-436
/
2013
Bone homeostasis is maintained by co-ordination of bone-resorbing osteoclasts and bone-forming osteoblasts. Imbalance between osteoclasts and osteoblasts leads to many bone diseases such as osteoporosis, rheumatoid arthritis. Taxillus chinensis is a herb that has been widely used to improve bone health. However, the effect and mechanism of Taxillus chinensis extract on osteoclast differentiation and bone resportion has been unknown. Thus, We investigated the effect of Taxillus chinensis on expression of receptor activator of nuclear factor-${\kappa}B$ ligand (RANKL)-induced osteoclast differentiation and bone resorption. Also, the action of Taxillus chinensis on mechanisms relating to osteoclast differentiation was studied. In this results, we identified that Taxillus chinensis significantly inhibited RANKL-induced osteoclast differentiation and bone resportion. Moreover, Taxillus chinensis was suppressed the activation of NF-${\kappa}B$ in bone marrow macrophage treated RANKL and M-CSF. Taxillus chinensis was down-regulated the mRNA expression of c-Fos, nuclear factor of activated T-cells (NFAT)c1, osteoclast-associated receptor (OSCAR), tartrate-resistant acid phosphatase (TRAP). The cell adhesion-related molecules such as integrin ${\alpha}v$ and integrin ${\beta}3$, and the filamentous actin (F-actin) rings of mature osteoclasts-related molecules such as dendritic cell-specific transmembrane preotein (DC-STAMP) and cathepsin K are also suppressed. Taken together, these results indicated that Taxillus chinensis will be a good candidate to treat osteoclast-mediated bone diseases.
Journal of the Korean Society for Library and Information Science
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v.42
no.3
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pp.61-79
/
2008
The purpose of this paper is to investigate the three aspects of the construction and common use of old document DB in the foreign countries: i) the processing of old documents, ii) the problem and improvement of DB systems of old documents. and iii) the common use of old document DB. Results from this research are as follows: The National Library of Korea(NLK) copied old documents in the foreign countries from 1982 to 2006 and published the brief catalog. The Reogang Publishing company issued four volumes catalogs of old document in Japan. The National Research Institute of Cultural Heritage(NRICH) investigated old books and published some catalogs of several organizations in Japan. America. France. and all. The National Institute of Korean History(NIKH) investigated old archives and published some catalogs of several organizations in Japan. The characteristics of the Korean Old and Rare Collection Information System(KORCIS) of the NLK, the Old Books Cultural Heritage in Overseas System of the NRICH. and the Korea History DB System and MF Catalog/ Image System of NIKH were described in the DB systems of old documents, the problems of DB systems were checked over and some alternatives were suggested. In the common use of old document DB, KORMARC format and description rules(draft) for archives should be revised to adopt a new standard such as KS editions. and all the institutes involved should thoroughly follow the standards. when creating bibliographic records and digitizing texts. It is necessary to educate and train the specialists of old documents. A government organization should be established to supervise all the procedures of developing technology for sharing digitized resources. using contents. and cooperating with the related internationl organizations and institutes.
Polymorphisms of inflammation-related genes have been found to be associated with non-Hodgkin lymphoma (NHL) or some of its subtypes, but only a few relevant data have been reported in China. In this study, the Snapshot method was used to assess genetic variation; a total of 14 single nucleotide polymorphisms (SNPs) for 6 inflammatory factors in 157 NHL cases (64 Uygur ethnic subjects, 93 Han Chinese) and 435 controls (231 Uygur and 204 Han Chinese) were studied from the Xinjiang province of China. Haplotype distribution was estimated using PHASE 2.3 software. Statistical differences in the genotype and haplotype frequencies between case and control groups were also considered and estimated. For the Han population, the geneotype distributions for TNF-${\alpha}rs1800629$, TNF-${\alpha}rs1800630$, IL-6 rs1800795, IL-6 rs1800797, NF-KB1 rs1585215 and TLR-4 rs4986790 showed significant differences between the case and control groups (p<0.05). The TNF-${\alpha}$ gene frequencies of ACG and CCA haplotypes in the cases were higher than in the controls (OR=2.45, 95% CI: 1.55-3.89, p=0.0002, OR=2.53, 95% CI: 1.10-5.80, p=0.029, respectively), and the same findings were detected for TNF-${\beta}$ gene CA haplotype (OR=1.87, 95% CI: 1.21-2.90, p=0.0054). However, for the Uygur population, no such significant differences were detected within the gene-type distribution of the 14 SNPs. The TNF-${\alpha}$ gene frequency of the CCA haplotype between the two groups (OR=1.98, 95% CI: 1.11-3.51, p=0.021) revealed a statistically significant difference. Our results showed that polymorphic variations of inflammation-related genes could be important to the NHL etiology of the Han population, and that these may only have limited influence on the Uygur population.
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