• Korean Journal of Poultry Science
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• v.40 no.3
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• pp.171-178
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• 2013

The purpose of this study was to evaluate the sperm viability, normal acrosome and mitochondrial activity in the frozen-thawed fowl semen by different cryoprotectants. The experiment was carried out on 10 sexually adult roosters of Ogye. The semen was collected twice a week and pooled semen was diluted 1:1 EK extender containing no cryoprotectant at $5^{\circ}C$. After equilibration for 30 minutes, diluted chicken semen was diluted 1:1 extender containing either 7% dimethylacetamide (DMA), 7% dimethylformamide (DMF) or 7.5% methylacetamide (MA) at final concentration and was put in 0.5 mL plastic straws and frozen for 30 minutes by exposure to liquid nitrogen vapor 4 cm above the surface of liquid nitrogen, followed by plunging into liquid nitrogen. Frozen semen was thawed in water bath at $5^{\circ}C$ for 2 minutes. For cytometric analysis, the frozen-thawed semen was diluted with EK extender to a final concentration of 90 million spermatozoa per mL. Sperm membrane integrity was evaluated as SYBR-14 and propidium iodide (PI). Acrosome integrity was assessed with fluorescein isothiocyanate-labeled PSA and PI. The percentage of mitochondrial function was estimated by using Rhodamine123 (R123) and PI. In conclusion, freezing rooster semen by using 7% DMF as cryoprotectant was significantly highest in rates of survival and mitochondrial function while its rate of damage of acrosome was significantly lowest. As a result, DMF is the cryoprotectant that has the lowest influences on sperm membranes and acrosome integrity. Therefore it could be used for freezing method of animal genetic conservation method for poultry diversity.

• Journal of Korean Society of Environmental Engineers
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• v.28 no.5
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• pp.463-471
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• 2006

Electrical resistivity surveys were conducted at areas of abandoned landfills in Cheonan and Wonju. Geology and extent of leachate migration around the landfills were evaluated with collected resistivity data by 2-D and 3-D resistivity inverse modeling. The Cheonan landfill is located above the paddy fields and the resistivity survey lines were crossed to examine possible pollution at the paddy fields by leakage of the landfill leachate. In Wonju, the landfill and the downgradient paddy fields are divided by a concrete barrier wall. At the bottom of the landfill, there is a leachate settlement system, which has not been in operation. To evaluate leachate leakage into the paddy fields, a total of 4 survey lines were used. According to the resistivity survey results, the landfill leachate in Cheonan appeared to be restricted only within the interior of the landfill, not to migrate into the subsurface of the paddy fields. These results are well consistent with electrical conductivity values of groundwaters obtained from a periodic analysis of water qualities. In Wonju, however, it was inferred that the leachate emanating from the landfill migrated beneath the abandoned leachate settlement system and the leachate would reach the downgradient paddy fields. Low resistivity area was observed in the old reservoir area and it appeared to be derived from convergence of groundwater flows from the surrounding valley and the moist wet land. In addition, groundwater flow into the paddy fields occurs beneath the old reservoir embankment at depths of $7{\sim}8m$. This paper reports details of the resistivity surveys for the uncontrolled landfills.

• Journal of the Korean earth science society
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• v.38 no.1
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• pp.24-34
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• 2017

The westerly waves generation is described in the advanced earth science textbook used at high school as follows: as westerly wind approaches and blows over large mountains, the air flow shows wave motions in downwind side, which can be explained by the conservation of potential vorticity. However, there has been no case study showing the phenomena of the mesoscale westerly waves with observational data in the area of small mountains in Korea. And thus the wind speed and time persistency of westerly winds along with the width and length of mountains have never been studied to explain the generation of the westerly waves. As a first step, we assured the westerly waves generated in the downwind side of Sobaek mountains based on surface station wind data nearby. Furthermore, the critical or minimum wind velocity of the westerly wind over Sobaek mountains to generate the downwind wave were derived and calcuated tobe about $0.6m\;s^{-1}$ for Sobaek mountains, which means that the westerly waves could be generated in most cases of westerly blowing over the mountains. Using surface station data and 4-dimensional assimilation data of RDAPS (Regional Data Assimilation and Prediction System) provided by Korea Meteorological Agency, we also analyzed cases of westerly waves occurrence and life cycle in the downwind side of Sobaek mountains for a year of 2014. The westerly waves occurred in meso-${\beta}$ or -${\gamma}$ scales. The westerly waves generated by the mountains disappeared gradually with wind speed decreasing. The occurrence frequency of the vorticity with meso-${\beta}$ scale got to be higher when the stronger westerly wind blew. When we extended the spatial range of the analysis, phenomena of westerly waves were also observed in the downwind side of Yensan mountains in Northeastern China. Our current work will be a study material to help students understand the atmospheric phenomena perturbed by mountains.

• Journal of the Korean Geographical Society
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• v.40 no.3 s.108
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• pp.253-273
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• 2005

In recent years, there are strong social demands to characterize the spatial distribution of mountains in Korea. This study aims to develop a 'Sanjulgi-Jido(mountain ridge map)' that might be used not only to satisfy these social demands but also to effectively present the spatial distribution of mountains and drainage basins in the Korean Peninsular. The 'Sanjulgi-Jido' developed in this study is a map that presents the continuity of mountains based on the drainage divides that are delineated by a pre-defined drainage basin size and elevation. This study first validated the Bakdudaegan system through the analyses of a digital elevation model. The Bakdudaegan system has long been recognized as the Koreans traditional conceptual framework to characterize the spatial distribution of mountains. The analyses showed that the Bakdudaegan system has several problems to represent the mountain systems in Korea, which includes 1) the lack of the representativeness of drainage basins, 2) inaccuracy to depict the boundary of drainage basins, 3) the lack of representativeness of mountains, and 4) geo-polical issue that confines the spatial extent of mountain systems within the Korean Peninsular. In order to represent the mountains system in a more quantitative manner, we applied several terrain analysis techniques to understand the spatial distribution of mountains and drainage basins. Based on these analyses, we developed an hierarchical system to classify the continuity (If mountains, which are presented as the spatial distribution of drainage divides with a certain elevation. The first-order Sanjulgi is the drainage divides whose drainage basin are bigger than $5,000km^2$ and the point elevation is above 100m. The next order Sanjulgi is delineated as the size of drainage basin is successively divided by two. This kind of design is able to provide a logical framework to present the mountain systems at different details, depending on the purpose and scale of maps. We also provide several empirical functions to calculate various geomorphological indices for each order of Sanjulgi. The 'Sanjulgi Jido' is similar with the Bakdudaegan system, since it characterizes the continuity of mountains based on the spatial distribution of the drainage divide. It, however, has more scientific criteria to define the scale and continuity of mountains. It should be also noted that the 'Sanjulgi Jido' proposed has different logical and methodological background, compared with the mountain range map that explains the genesis of mountain systems in addition to the continuity of mountains.

• Clean Technology
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• v.20 no.1
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• pp.42-50
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• 2014

In this study, the $Mg(OH)_2$ slurry was made form ferro-nickel slag and then used for $CO_2$ sequestration. The experiments were in the order as leaching step, precipitation, carbonation experiments. According to the leaching results, the optimal leaching conditions were $H_2SO_4$ concentration of 1 M and the temperature of 333 K. In the $Mg(OH)_2$ manufacturing step, NaOH was added to increase the pH upto 8, the first precipitation was confirmed as $Fe_2O_3$. After removal the first precipitation, the pH was upto 11, the $Mg(OH)_2$ was generated by XRD analysis. The $Mg(OH)_2$ slurry was used for $CO_2$ sequestration. The pseudo-second-order carbonation model was used to apply for $CO_2$ sequestration. The $CO_2$ sequestration rate was increased by the $CO_2$ partial pressure and temperature. However, $CO_2$ sequestration rate was decreased when temperature upto 323 K. After $CO_2$ sequestrated by $Mg(OH)_2$, the $CO_2$ can be sequestrated stable as $MgCO_3$. This study also presented optimal sequestration condition was the pH upto 8.38, the maximum $MgCO_3$ can be generated. This study can be used as the basic material for $CO_2$ sequestration by ferro-nickel slag at pilot scale in the future.

• Korean Journal of Environmental Biology
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• v.25 no.2
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• pp.168-177
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• 2007

By using DEM and digital actual vegetation map with MGE GIS software program, topographic features (altitude, slope, latitude, etc.) quantitatively were analysed and their data integrated as the index of climatic conditions (WI, CI, air temperature, etc.) in South Korea. Warmth Index (WI) decreases $5.27^{\circ}C{\cdot}month$ with latitudinal $1^{\circ} degree, and $3.41^{\circ}C{\cdot}month$ with attitudinal 100 m increase. The relationship between CI and WI values is expressed as a linear regression, $WI=116.01+0.96{\times}CI,\;R^2=0.996$. The distributional peaks of different plant communities along Warmth Index gradient showed the sequence of Abies nephrolepis, Taxus cuspidata, Abies koreana, Quercus mongolica, Carpinus laxiflora, Q. dentata, C. tschonoskii, Q. serrate, Pinus densiflora, Q. aliena, Q. variabilis, Q. acutissima, P. thunbergii, Q. acute, Castanopsis cuspidata var. sieboldii, Camellia japonica, Machilus thunbergii community from lower to higher values. The Quercus mongolica forest occurred frequently on E-NW and SE slope aspect within WI $70{\sim}80^{\circ}C{\cdot}month$ optimal range at mesic sites, NW and SE slope than xeric sites S and SW slope. The Q. serrata forest showed the most distributional frequency in NW and W slope aspect within WI $90{\sim}100^{\circ}C{\cdot}month$ range, Q. variabilis and Q. acutissima forest showed the high frequency of distribution in SE slope in WI $95{\sim}100^{\circ}C{\cdot}month$ range. By the slope gradient analysis, five groups were found: 1. Abies nephrolepis, Machilus thunbergii, 2. Taxus cuspidata, Abies koreana, Quercus mongolica, Q. dentata, Q. serrata, Q. variabilis, Castanopsis cuspidata var. sieboldii 3. Pinus densiflora, Q. aliena, Q. acutissima, P. thunbergii, Q. acuta 4. Carpinus laxiflora, Camellia japonica 5. C. tschonoskii from steep slope to gentle slope sequence.

• Korean Journal of Soil Science and Fertilizer
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• v.33 no.2
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• pp.71-78
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• 2000

Preferential flow has recently been the subject of increasing interest because these phenomena contribute to solute transport in soils. Commonly, preferential flow paths are associated with macropores or highly structured soils. We presented an analysis of the measured breakthrough curves (BTCs) of $Cl^-$ and $Cu^{2+}$ ions to test the occurrence of preferential flow in soils using miscible displacement technique under steady flow conditions. We also analyzed soil water retention curves and from this curves induced cumulative pore size distribution of undisturbed soils, which sampled from Ap1, B1, and C horizons of Songjeong series soils (the fine loamy, mesic family of Typic Hapludults). In this study, miscible displacement experiment on C horizon was excluded, because it is structureless sandy loam with saturated hydraulic conductivity of $5.2cmhr^{-1}$. The saturated hydraulic conductivity of Ap1 horizon was $2.0cmhr^{-1}$, which was about 7 times higher than that of B1 horizon ($0.27cm hr^{-1}$). Cumulative pore size distribution predicted that Ap1 horizon had more macropores (pore diameter larger than $49{\mu}m$, equivalent to -6 kpa of soil matric potential) than B1 horizon. The hydrodynamic dispersion coefficient from chloride BTCs was estimated as $1.3cm^2hr^{-1}$ for B1 and $34cm^2hr^{-1}$ for Ap1 horizon. However the retardation factors of B1 and Ap1 horizon were significantly different, i.e. 1 and 0.6, respectively, which means that there was distinct partition between mobile water and immobile phase in Ap1 horizon. The copper retardation effect of Ap1 horizon was less than that of B1 horizon, even though cation exchange capacity of Ap1 horizon was higher than that of B1 horizon. Thus, breakthrough curves of $Cl^-$ and $Cu^{2+}$ obviously showed the probability that preferential flow would occur in Ap1 horizon.

• Journal of the Korean Society of Radiology
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• v.4 no.1
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• pp.5-9
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• 2010

In recent, a digital x-ray detector attracted worldwide attention and there are many studies to commercialize. There are two methods in digital x-ray detector. This method is an Indirect method and Direct method. This study is to see the differences between the digital x-ray detector based on a-Se used in the existing indirect conversion method and an x-ray conversion material that has better SNR(Signal-to-noise ratio) and property than the a-Se. To solve the problem that is difficult to make a large area film using Screen-Print method, we used a Screen-Print method. In this study, we used a polyclystal $HgI_2$ as x-ray conversion material and a sample thickness is $150{\mu}m$ and an area is $3cm{\times}3cm$. ITO(Indium-Tin-Oxide) electrode was used as top electrode using a Magnetron Sputtering System and each area is $3cm{\times}3cm$, $2cm{\times}2cm$ and $1cm{\times}1cm$ and then we evaluated darkcurrent, sensitivity and SNR of the $HgI_2$ film are measured, then we evaluated the electrical properties. And we used a current integration mode when I-V test. This experiment shows that the sensitivity increases in accordance with the area of the electrode but the SNR is decreased because of the high darkcurrent. Through fabricating of various thicknesses and optimal electrodes, we will optimize SNR in the future work.

• Horticultural Science & Technology
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• v.28 no.3
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• pp.403-408
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• 2010

This study was carried out to investigate the physiological responses of $Rhododendron$ $mucronulatum$ Turcz. and $R.$ $indicum$ (L.) Sweet seedlings with 0%, 35%, 55% and 75% shading of full sunlight in polyethylene film house. The shading treatments were performed during the late growth season for each species (from Sept. 9 to Nov. 5, 2008). The shading treatment was effective in reducing the daily temperature by 0.9 to $1.7^{\circ}C$ during September and by 0.8 to $1.7^{\circ}C$ during October. Before the shading treatments, the water content of $R.$ $mucronulatum$ and $R.$ $indicum$ amounted to 68.5% and 66.3%, respectively. The water contents of two species after 75% shading treatment period decreased to 66.2% (3.4% reduction) and 65.9% (0.6% reduction), respectively. Notably, both species had a similar tendency indicating less reduction rate of water content with 75% shading. $R.$ $indicum$ showed higher photosynthetic capacity with higher level of shading, and its photosynthetic capacity reached the highest level ($9.63{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$). On the other hand, shading-treated $R.$ $indicum$ showed higher intercellular $CO_2$ concentration, stomatal conductance and transpiration rate (55% shading > 35% shading > 75% shading) than non-treated ones. In addition, non-treated seedlings showed higher water use efficiency than treated ones. In particular, it was found that the leaf color of $R.$ $mucronulatum$ turned equivalent to purple under full sunlight, while its leaf color kept equivalent more to green with higher level of shading, as evidenced even in naked eyes. According to comprehensive analysis using Munsell Color Chart on potential leaf color variations of $R.$ $mucronulatum$ depending on the level of shading, it was found that relatively many leaf colors under full sunlight were equivalent to R (red) and Y (yellow) chart, while relatively many leaf colors with higher level of shading were equivalent to G (green) and Y chart, where the latter still showed green color.

• Korean Journal of Environmental Biology
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• v.35 no.4
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• pp.694-705
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• 2017

The aim of this study was to isolate and identify marine bacterium with anti-methicillin-resistant Staphylococcus aureus (MRSA) activity, and to purify the anti-MRSA compound, as well as to determine its activity and synergistic effects. Among the marine bacteria isolated in this study, the YJ-1 isolate had the strongest anti-MRSA activity. The YJ-1 isolate was identified on the basis of its biochemical characteristics and an analysis of 16S rRNA gene sequences. The YJ-1 isolate showed over 99.2% homology with Pseudomonas stutzeri, and was designated as a Pseudomonas sp. YJ-1. The optimal culture conditions were $25^{\circ}C$ and initial pH 7.0. For the purification of the anti-MRSA compounds, the YJ-1 was cultured in Pa PES-II medium, and the culture filtrates were extracted by ethyl acetate, hexane, and 80% MeOH. The 80% MeOH fraction was separated by a $C_{18}$ ODS column, silica gel chromatography and a reverse phase HPLC, to yield three anti-MRSA agents, the MR1, MR2, and MR3 compounds. When the MR1 compound of $250{\mu}g\;mL^{-1}$ concentration was applied to the MRSA cells, over 95% of bacterial cells was killed within 48 hr. Compared with vancomycin and ampicillin, the MR1 compound showed significant anti-MRSA activity. In addition, the anti-MRSA activity was increased by dose and time dependent manners. Furthermore, the combination of an MR1 compound with vancomycin produced a more rapid decrease in the MRSA cells than did the MR1 compound alone. Taken together, our results suggest that the Pseudomonas sp. YJ-1 and its anti-MRSA compounds could be employed as a natural antibacterial agent in MRSA infections.