• Journal of Korean Ophthalmic Optics Society
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• v.20 no.4
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• pp.501-509
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• 2015

Purpose: To evaluate the reliability of refractive power by comparing the marked refractive power in an automatic phoropter and actually measured spherical/cylindrical refractive power. Methods: Actual refractive power of minus spherical lens and cylindrical lens in an automatic phoropter was measured by a manual lensmeter and compared with the accuracy of marked refractive power. Furthermore, combined refractive power and spherical equivalent refractive power of two overlapped lenses were compared and evaluated with the refractive power of trial lens. Results: An error of 0.125 D and more against the marked degree was observed in 70.6% of spherical refractive power of spherical lens which is built in phoropter, and the higher error was shown with increasing refractive power. Single cylindrical refractive power of cylindrical lens is almost equivalent to the marked degree. Combined spherical refractive power was equivalent to spherical refractive power of single lens when spherical lens and cylindrical lens were overlapped in a phoropter. Thus, there was no change in spherical refractive power by lens overlapping. However, there was a great difference, which suggest the effect induced by overlapping between cylindrical refractive power and the marked degree when spherical lens and cylindrical lens were overlapped. Spherical equivalent refractive power measured by using a phoropter was lower than that estimated by trial glasses frame and marked degree. The difference was bigger with higher refractive power. Conclusions: When assessment of visual acuity is made by using an automatic phoropter for high myopes or myopic astigmatism, some difference against the marked degree may be produced and they may be overcorrected which suggests that improvement is required.

• KSBB Journal
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• v.16 no.2
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• pp.188-199
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• 2001

A gene encoding the dextransucrase(dsCB) that synthesizes mostly $\alpha-(1\rightarrow6)$ linked dextran with low amount(10%) of $\alpha-(1\rightarrow3)$ branching was cloned and sequenced from Leuconostoc mesenteroides B-742CB. The 6.1 kbp DNA fragment carrying dsCB showed one open reading frame(ORF) composed of 4,536bp. The deduced amino acid sequence shows that it begins from the start codon(ATG) at position 698 of the cloned DNA fragment and extends to the termination condon(TAA) at position 5,223. The enzyme is consisted of 1,508 amino acids and has an calculated molecular mass of 168.6kDa. This calculated Mw was in good agreement with an activity band of 170kDa on non-denaturing SDS-PAGE. A recombinant E. coli DH5 $alpha$ harboring pDSCB produced extracellular dextransucrase in 2% sucrose medium, and synthesized both soluble and insoluble dextran. To compare the properties of enzyme with B-742CB dextransucrase, the acceptor reaction, hydrolysis of dextran and methylation were performed. The expressed enzyme showed the same properties as B-742CB dextransucrease, but its ability to synthesize $\alpha-(1\rightarrow3)$ branching was lower than that of B-742CB dextransucrase. In order to identify the critical amino acid residues known as conserved regions related to catalytic activity, Asp-492 was replaced with Asn. D492N resulted in a 1.6 fold decrease in specific activity.

• Radiation Oncology Journal
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• v.17 no.1
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• pp.84-88
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• 1999

Purpose :To develop a method for verifying a treatment setup in stereotactic radiotherapy by ma- tching portal images to DRRs. Materials and Methods : Four pairs of orthogonal portal images of one patient immobilized by a thermoplastic mask frame for fractionated stereotactic radiotherapy were compared with DRRs. Portal images are obtained in AP (anteriorfposterior) and lateral directions with a target localizer box containing fiducial markers attached to a stereotactic frame. DRRs superimposed over a planned iso-center and fiducial markers are printed out on transparent films. And then, they were overlaid over onhogonal penal images by matching anatomical structures. From three different kind of objects (isgcenter, fiducial markers, anatomical structure) on DRRs and portal images, the displacement error between anatomical structure and isocenters (overall setup error), the displacement error between anatomical structure and fiducial markers (irnrnobiliBation error), and the displacement error between fiducial markers and isocenters (localization error) were measured. Results : Localization error were 1.5$\pm$0.3 mm (AP), 0.9$\pm$0.3 mm (lateral), and immobilization errors were 1.9$\pm$0.5 mm (AP), 1.9$\pm$0.4 mm (lateral). In addition, overall setup errors were 1.0$\pm$0.9 mm (AP), 1.3$\pm$0.4 mm (lateral). From these orthogonal displacement errors, maximum 3D displacement errors($\sqrt{(\DeltaAP)^{2}+(\DeltaLat)^{2}$)) were found to be 1.7$\pm$0.4 mm for localization, 2.0$\pm$0.6 mm for immobilization, and 2.3$\pm$0.7 mm for overall treatment setup. Conclusion : By comparing orthogonal portal images with DRRs, we find out that it is possible to verify treatment setup directly in stereotactic radiotherapy.

• Korean journal of applied entomology
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• v.35 no.4
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• pp.321-325
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• 1996

To isolate a novel gene for antibacterial peptide, an inducible clone(BmInc8) was selected by differential screening strategy from Bombyx mori cDNA library prepared from lavae injected with Escherichia coli. This clone contained a cDNA insert of 564 nucleotides and encoded 59 amino acids with an apparent molecular mass of 6.3 kDa. The cDNA sequence of BmInc8 had 61.2% identity compared to that of the bactericidin from Manduca sexta and also the deduced amino acids sequences from this insert had 65% identity compared to that of the cecropin D peptide Hyalophora cecropia. The transient expression assay of this insert using prokaryotic expression vector system revealed that the expressed peptide displayed the antibacterial activity. The cDNA sequence was deposited in GenBank under the accession number U30289.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.36 no.5B
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• pp.517-529
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• 2011

In this paper, the parameters of the RTT (Radio Transmission Techniques) for SANET (Ship Ad-hoc NETwork), which is considered for the next generation maritime communication systems, are set up. A channel model has been analyzed based on the practical measured maritime wireless channel in VHF (Very-High Frequency) for SANET system. Also, by considering the frame structure including preamble, guard time and pilots for both single and multi-carrier systems, the BER (Bit Error Rate) performances are evaluated and analyzed in the aspects of channel compensation and channel coding techniques. Based on the simulation results, optimal modulation & coding schemes are suggested for SANET. That is, in single-carrier system by using differential modulation schemes, channel compensation is not necessary. However, channel coding is helpful to achieve additional gain. On the other hand, when 16-QAM modulation is employed in multi-carrier system, the implementation of both channel compensation and channel coding techniques show huge performance gain for various of K values, which are related to different maritime environments, and the rolling effects of wave.

• Journal of the Korean School Mathematics Society
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• v.13 no.2
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• pp.303-322
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• 2010

The purpose of this study was to analyze the effects of using the geometric manipulative for the development of spatial sense and thus to find out a better mathematics teaching and learning method that could help develop students' spatial senses. The two fifth grade classes were randomly chosen as an experimental group (31 students) and a control group (32 students), respectively. This study implemented nonequivalent control group pretest-posttest design of quasi-experimental design. The test instrument used in this study was a spatial sense test. The pretest and posttest were implemented with the same instrument. In addition, their classes were observed and videotaped, and the data and their study activities were analyzed. In conclusion, first, the geometric manipulative-aided activities contributes to developing students' spatial senses and their two sub-factors involves perceptual consistency and perception of spatial relationship. Second, the activities of grasping the components of solid figures, sketches and development figures by using the geometric manipulative contribute to boost students' perceptual consistencies and their perceptions of spatial relationship.

• Journal of Microbiology and Biotechnology
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• v.17 no.1
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• pp.37-43
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• 2007

A gene encoding inulin fructotransferase (di-D-fructofuranose 1,2': 2,3' dianhydride [DFA III]-producing IFTase, EC 4.2.2.18) from Bacillus sp. snu-7 was cloned. This gene was composed of a single, 1,353-bp open reading frame encoding a protein composed of a 40-amino acid signal peptide and a 410-amino acid mature protein. The deduced amino acid sequence was 98% identical to Arthrobacter globiformis C11-1 IFTase (DFA III-producing). The enzyme was successfully expressed in E. coli as a functionally active, His-tagged protein, and it was purified in a single step using immobilized metal affinity chromatography. The purified enzyme showed much higher specific activity (1,276 units/mg protein) than other DFA III-producing IFTases. The recombinant and native enzymes were optimally active in very similar pH and temperature conditions. With a 103-min half-life at $60^{\circ}C$, the recombinant enzyme was as stable as the native enzyme. Acidic residues and cysteines potentially involved in the catalytic mechanism are proposed based on an alignment with other IFTases and a DFA IIIase.

• Journal of Microbiology and Biotechnology
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• v.12 no.2
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• pp.301-305
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• 2002

The gene (ppaT) encoding Thermus caldophilus GK24 pyrophosphatase (Tca pyrophosphatase) was cloned and sequenced. The gene was found to contain an open reading frame encoding 175 amino acids with a calculated mass of 19,155 Da. The ppaT gene was expressed under the control of the tac promoter in Escherichia coli. The recombinant Tca pyrophosphatase was purified 21.4-fold with $56\%$ yield and specific activity of 25.7 U $mg^-1$, following a combination of heating (to denature the E. coli proteins) and one step of DEAE-Sephacel column chromatography. The native enzyme was found to have an approximate molecular mass of 110,000 Da and consisted of six subunits. The enzyme exhibited maximal activity at pH of 8.0-8.5 and was stable at $80-90^{\circ}C$. A divalent cation was absolutely required for the enzyme activity, with $Mg^2+$. being the most effective.

• Journal of the Optical Society of Korea
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• v.15 no.3
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• pp.293-299
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• 2011

In this study, we demonstrated Fourier-domain/swept-source optical coherence tomography (FD/SS-OCT) at a center wavelength of 800 nm for in vivo human retinal imaging. A wavelength-swept source was constructed with a semiconductor optical amplifier, a fiber Fabry-Perot tunable filter, isolators, and a fiber coupler in a ring cavity. Our swept source produced a laser output with a tuning range of 42 nm (779 to 821 nm) and an average power of 3.9 mW. The wavelength-swept speed in this configuration with bidirectionality is 2,000 axial scans per second. In addition, we suggested a modified zero-crossing method to achieve equal sample spacing in the wavenumber (k) domain and to increase the image depth range. FD/SS-OCT has a sensitivity of ~89.7 dB and an axial resolution of 10.4 ${\mu}m$ in air. When a retinal image with 2,000 A-lines/frame is obtained, an acquisition speed of 2.0 fps is achieved.

• Journal of the Korean Society of Costume
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• v.45
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• pp.85-102
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• 1999

The purpose of this study is reviewing and researching the origins of knitwear the history of patterned knits. Aithough historians know little about the origins of knitting many believe it was practiced as early as the 4th century by nomads roaming North Africa. later Arab raders adopted the craft which helped then while away the hours as they traveled across deserts in camel carabans, Its origins lie in the need for close-fitting and elasticated covering for the body in particular the head hand and feet. it first developed in the Mediterranean countries and later in Central and particularly Northern Europe. Early evidence of multicolored knitting is said to date back to the Egyptian Copts of 600-800 A.D. medieval knitting is developed through the Church and monastery. The increasing demand for knitted products already observable in the fourteenth and fifteenth centries and the number of preserved knitted articles increases inexcavated materialos from Europe. The improvements in technique stimulated the developement of the hand knitting industry in the early sixteenth century. The best-known source of production is the guild organization and their mass production consisted of the carpets cushion coverings and other small items for furnishing interiors but mainly of clothing. The demand for knitted goods was such that in the late sixteenth century it was mechanised, The knitting frame invented in 1589 by William Lee English priest was the most perfect machine of this period. The mass production of fully-fashioned and seamless garments in the late nineteenth and twentieth century was dangerously competitve to traditionally woven and sewn cloth in. As fashions changed knitwear has had an almost continuous ruse in public favour and the popularity of sports has encourage the fashion for flexible easy-fitting and absorbent garments.