• 한국식물학회:학술대회논문집
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• 한국식물학회 1999년도 제13회 식물생명공학심포지움 New Approaches to Understand Gene Function in Plants and Application to Plant Biotechnology
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• pp.57-62
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• 1999

Light-regulated translation of chloroplast mRNAs requires nuclear-encoded trans-acting factors that interact with the 5' untranslated region (UTR) of these mRNAs. A set of four proteins (60, 55, 47, and 38 kDa) that bind to the 5'-UTR of the psbA mRNA had been identified in C. reinhardtii. 47 kDa protein (RB47) was found to encode a chloroplast poly (A)-binding protein (cPABP) that specifically binds to the 5'-UTR of the psbA mRNA, and essential for translation of this mRNA, cDNA encoding 60 kDa protein (RB60) was isolated, and the amino acid sequence of the encoded protein was highly homologous to plants and mammalian protein disulfide isomerases (PDI), normally found in the endoplasmic reticulum (ER). Immunoblot analysis of C. reinhardtii proteins showed that anti-PDI recognized a distinct protein of 56 kDa in whole cell extract, whereas anti-rRB60 detected a 60 kDa protein. The ER-PDI was not retained on heparin-agarose resin whereas RB60 was retained. In vitro translation products of the RB60 cDNA can be transported into C. reinhardtii chloroplast in vitro. Immunoblot analysis of isolated pea chloroplasts indicated that higher plant also possess a RB60 homolog. In vitro RNA-binding studies showed that RB60 modulates the binding of cPABP to the 5'-UTR of the psbA mRNA by reversibly changing the redox status of cPABP using redox potential or ADP-dependent phosphorylation. Site-directed mutagenesis of -CGHC- catalytic site in thioredoxin-like domain of RB60 is an unique PDI located in the chloroplast of C. reinhardtii, and suggest that the chloroplast PDI may have evolved to utilize the redox-regulated thioredoxin like domain as a mechanism for regulating the light-activated translation of the psbA mRNA.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1985년도 워크샵 및 심포지엄 북한산국립공원의 식생
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• pp.7-18
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• 1985

Light-regulated translation of chloroplast mRNAs requires nuclear-encoded trans-acting factors that interact with the 5' untranslated region (UTR) of these mRNAs. A set of four proteins (60, 55, 47, and 38 kDa) that bind to the 5'-UTR of the psbA mRNA had been identified in C. reinhardtii. 47 kDa protein (RB47) was found to encode a chloroplast poly (A)-binding protein (cPABP) that specifically binds to the 5'-UTR of the psbA mRNA, and essential for translation of this mRNA, cDNA encoding 60 kDa protein (RB60) was isolated, and the amino acid sequence of the encoded protein was highly homologous to plants and mammalian protein disulfide isomerases (PDI), normally found in the endoplasmic reticulum (ER). Immunoblot analysis of C. reinhardtii proteins showed that anti-PDI recognized a distinct protein of 56 kDa in whole cell extract, whereas anti-rRB60 detected a 60 kDa protein. The ER-PDI was not retained on heparin-agarose resin whereas RB60 was retained. In vitro translation products of the RB60 cDNA can be transported into C. reinhardtii chloroplast in vitro. Immunoblot analysis of isolated pea chloroplasts indicated that higher plant also possess a RB60 homolog. In vitro RNA-binding studies showed that RB60 modulates the binding of cPABP to the 5'-UTR of the psbA mRNA by reversibly changing the redox status of cPABP using redox potential or ADP-dependent phosphorylation. Site-directed mutagenesis of -CGHC- catalytic site in thioredoxin-like domain of RB60 is an unique PDI located in the chloroplast of C. reinhardtii, and suggest that the chloroplast PDI may have evolved to utilize the redox-regulated thioredoxin like domain as a mechanism for regulating the light-activated translation of the psbA mRNA.

• 한국안광학회지
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• 제13권3호
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• pp.13-17
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• 2008

목적: 안경광학의 미래를 전망하기 위함에 있다. 방법: 안경광학과 3년제 1학년, 2학년, 3학년 각각 100명을 대상으로 의식조사를 하였다. 결과: 설문조사 결과, 1. 입학 전 안경광학과에 대한 사전정보 인지도는 1학년 40명(40%)에서 보통으로, 2학년 42명(42%)에서 보통으로, 3학년 34명(34%)에서 낮음으로 조사되었다. 2. 안경광학과 수강과목과 내용 적합도는 1학년 54명(54%)에서 보통으로, 2학년 51명(51%)에서 적당으로, 3학년 47명(47%)에서 적당으로 조사되었다. 3. 안경광학과 선택의 만족도는 1학년 43명(43%)에서 높음으로, 2학년 45명 (45%)에서 보통으로, 3학년 41명(41%)에서 높음으로 조사되었다. 4. 안경광학과 수업의 이해도는 1학년은 55명(55%)에서 보통으로, 2학년은 60명(60%)에서 보통으로, 3학년은 49명(49%)에서 높음으로 조사되었다. 5. 안경광학과 교우 관계의 만족도는 1학년 40명(40%), 2학년 50명(50%), 3학년 51명(51%)에서 높음으로 조사되었다. 6. 안경광학과 선후배 관계의 만족도는 1학년 47명(47%), 2학년 50명(50%), 3학년 41명(41%)에서 보통으로 조사되었다. 7. 안경광학과 미래전망은 1학년 47명(47%), 2학년 50명(50%), 3학년 45명(45%)에서 보통으로 조사되었다. 8. 안경광학과 졸업 후 진로는 1학년 60명(60%), 2학년 68명(68%), 3학년 66명(66%)에서 안경원으로 조사되었다. 결론: 학과 선택의 만족도와 미래전망은 대체적으로 좋은 것으로 조사되었다.

• Journal of Periodontal and Implant Science
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• 제47권3호
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• pp.174-181
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• 2017

Purpose: Epitope spreading is a phenomenon in which distinct subdominant epitopes become major targets of the immune response. Heat shock protein (HSP) 60 from Porphyromonas gingivalis (PgHSP60) and peptide 19 from PgHSP60 (Pep19) are immunodominant epitopes in autoimmune disease patients, including those with periodontitis. It remains unclear whether Pep19 is a dominant epitope in subjects without periodontitis or autoimmune disease. The purpose of this study was to determine the epitope spreading pattern and verify Pep19 as an immunodominant epitope in healthy teenagers using dot immunoblot analysis. The patterns of epitope spreading in age-matched patients with type 1 diabetes mellitus (type 1 DM) and healthy 20- to 29-year old subjects were compared with those of healthy teenagers. Methods: Peptide from PgHSP60, Mycobacterium tuberculosis HSP60 (MtHSP60), and Chlamydia pneumoniae HSP60 (CpHSP60) was synthesized for comparative recognition by sera from healthy subjects and patients with autoimmune disease (type 1 DM). Dot immunoblot analysis against a panel of peptides of PgHSP60 and human HSP60 (HuHSP60) was performed to identify epitope spreading, and a densitometric image analysis was conducted. Results: Of the peptide from PgHSP60, MtHSP60, and CpHSP60, PgHSP60 was the predominant epitope and was most consistently recognized by the serum samples of healthy teenagers. Most sera from healthy subjects and patients with type 1 DM reacted more strongly with PgHSP60 and Pep19 than the other peptides. The relative intensity of antibody reactivity to Pep19 was higher in the type 1 DM group than in the healthy groups. Conclusions: Pep19 is an immunodominant epitope, not only in autoimmune disease patients, but also in healthy young subjects, as evidenced by their robust immunoreactivity. This result suggests that the Pep19-specific immune response may be an initiator that triggers autoimmune diseases.

• 한국축산식품학회지
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• 제39권6호
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• pp.863-876
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• 2019

The thermal-death times of Listeria monocytogenes were determined in inoculated restructured goat steak at 60℃, 65℃, and 70℃ of sous-vide temperatures. D-values of L. monocytogenes in inoculated restructured goat steak ranged from 7.27 min at 60℃ to 0.46 min at 70℃. Times need to yield at least a 6 log reduction of L. monocytogenes at their temperatures for this product were 47, 12, and 3 min, respectively. After sous-vide, all microbial counts in non-inoculated samples were not detectable, except the aerobic and anaerobic mesophilie and lactic acid bacteria counts were lower than 2 Log CFU/g. For sous-vided and grilled sous-vided samples, sous-vide loss and surface shrinkage were the lowest in samples sous-vided at 60℃ for 47 min (p<0.05). These samples demonstrated the lowest CIE L^*, shear force, hardness, gumminess and chewiness and the highest CIE a^* and hue angle (p<0.05). Therefore, sous-vide at 60℃ for 47 min provided convenient ready-to-cook restructured goat steak for microbiology safety and optimization of physicochemical quality.

• Journal of the Korean Wood Science and Technology
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• 제47권2호
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• pp.178-188
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• 2019

Licorice, which has an extensive history of use as an herbal medicine, has been suggested to have oral health benefits. However, to date, no systematic study has been conducted on the preparation method of licorice extracts for oral health. In this study, licorice extracts prepared using water and ethanol were investigated for its ability to inhibit the biofilm formation of Streptococcus mutans. The licorice extract prepared with around 60% ethanol effectively inhibited the biofilm formation of S. mutans. Licorice extracted with 50% ethanol almost completely inhibited the biofilm formation at 1.5 g/L of licorice extract. This inhibitory activity was confirmed in a microplate assay and a flow cell system. Glycyrrhetic acid was extracted from licorice effectively with 60% ethanol concentration. The strong inhibitory activity of glycyrrhetic acid and the synergistic inhibition with glycyrrhizin on biofilm formation were suggested as major reasons for a concentration-specific extraction. These results suggest that licorice extract prepared using around 60% ethanol effectively inhibits the biofilm formation of S. mutans.

• 한국자원식물학회지
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• 제22권2호
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• pp.123-127
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• 2009

실생묘 이앙으로 새로이 조성된 잔디밭에 발생되는 월년생 잡초의 종류 및 그 방제를 위한 특성을 조사한바 그 요지는 다음과 같다. 1. 발생잡초 종은 십자화과 및 국화과가 각각 29.4%이고, 석죽과 23.5%, 벼과 11.8%,꿀풀과 5.9%의 순위였다. 2. 발생 시기별로는 10월에 47.1%, 11월에 41.2%, 9월과 12월에 각각 5.9%를 나타내는 순위를 보인다. 3. 개화시기별로는 5월에 58.8%, 4월 23.5%, 3월 11.8%, 6월 5.9% 순위를 보였다. 4. 초장별로는 20-40 cm에 속하는 것이 47.1%, 20 cm 미만이 23.5%, 40-60 cm에 속하는 것이 17,6%, 60cm이상에 해당되는 것이 11.8%를 나타나고 있었다.

• 대한지질공학회:학술대회논문집
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• 대한지질공학회 2002년도 정기총회 및 학술발표회
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• pp.47-60
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• 2002

• 동아시아식생활학회지
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• 제26권4호
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• pp.334-345
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• 2016

This study conducted surveys on perception and utilization of Korean soybean paste targeting 493 women from their 20s to 60s residing in Daegu. Most subjects married (75.3%), were part of a nuclear family (84.8%), had more than a high school diploma (95.8%), had a career (47.9%), lived in an apartment (68.4%), had a total household income of 5 million won or more (38.1%), and had monthly food expenses more than 0.5 million won and less than 1 million won (42.2%). Regarding perception, 'rich nutrition' was the most commonly held perception in subjects in their 60s (4.4), followed by 'good taste' in subjects in their 40s (4.3). Regarding reasons for consumption 'healthy' was highest in subjects in their 60s (47.3%) and 40s (45.6%) and 'delicious' in subjects in their 20s (56.1%). Regarding how to make it, subjects in their 60s responded 'know for sure' (57.1%), while only 2% of 20s responded the same. Subjects in their 50s and 60s had a higher ratio of answering 'know' while 20s and 30s 'don't know', For preparation methods, subjects in their 20s (53.1%) and 30s (41.4%) answered 'buy at the market', subjects in their 40s (68.9%) 'get from family or relatives' and subjects in their 60s (47.3%) 'make by one's own'. For preservation method, subjects in their 20s and 30s answered 'preserve in refrigerator', the 60s tend to answer 'preserve in jangdokdae'. Buying at the market was most common in subjects in their 20s (36.1%) and 30s (28.5%) and the reason was 'convenient' (39.6%). The place of purchase was 'in large supermarket or department store' (78.5%). For the future consumption pattern, 'increase in consumption of commercialized product (44.0%)', subjects in their 60s answered 'keep making at home' while subjects in their 30s and 40s answered 'keep making at home or buying product half and half'. According to the above study results, women living in Daegu region were aware of excellence of traditional soybean paste. However, due to changes in residential circumstance and lack of knowledge about the recipe, they did not make. They receive soybean paste t from family, relatives or bought in the market, which indicates the continuous decrease in home-made soybean paste. Therefore, due to the expanding social participation of women, the product market is expected to increase. I think it is important to develop traditional commercial soybean paste in the traditional way to improve the traditional flavors.

• 한국지구과학회지
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• 제18권6호
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• pp.559-564
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• 1997

뉴헤브리디스 해분은 태평양판과 인도-오스트레일리아판의 경계부에 위치한 고령의 비활동적 비배호상 해분이다. 46 Ma에서 60 Ma 사이에 형성된 뉴헤브리디스 해분은 형성과정상 34 mm/a(42 Ma$\sim$47 Ma)와 17 mm/a(47 Ma$\sim$60 Ma)의 두가지 상이한 확장율을 보이며, 해분의 표층은 0.65 sec두께의 퇴적층으로 전반적으로 고르게 피복되어 있다. 본 연구에서 산출된 해분의 생장 발달 곡선은 $Depth(m)=2689+312\sqrt{Age}(Ma)$라는 수식으로 표현될 수 있으며, 여기서 냉각계수 312는 일반대양저의 350과 매우 유사함을 보이는 것이 특징이다. 이러한 특징은 해분의 암권의 생장 발달과정이 일반대양저와 유사할 가능성을 강력히 시사하고 있는 것으로 사료된다. 해분의 전반적인 후리-에어 중력 이상치는 -22.3 mgal에서 +59.0 mgal까지 심한 변화폭을 보이고 있다. 평균 중력이상치는 +30.2 mgal로 일반대양저에 비해 현저히 높은 값을 나타내고 있다. 이는 일반적으로 잘 나타나지 않는 매우 특이한 현상이다. 이것은 해분 자체가 섭입하는 판경계에 위치해 있어 marginal swell의 형상을 취하고 있기 때문인 것으로 해석된다. 이 swell들은 일반적으로 해구축상에서 대양저를 향해 약 200 km 범위내에서 관찰되어지며, 이들의 후리-에어 중력이상치는 평균 $+50{\sim}160mgal$을 보이는 것이 일반적이다. 해분의 높은 양의 중력이상은 이러한 지형적인 특수성에 기인하는 것으로 보여진다. 해분의 위치적 특성 및 암권 자체의 구부러짐에 따른 불안정성은 뉴 헤브리디스 해분의 대표적인 지질 지구물리학적 특징이라고 할 수 있다.