• Proceedings of the Botanical Society of Korea Conference
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• 1999.07a
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• pp.57-62
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• 1999

Light-regulated translation of chloroplast mRNAs requires nuclear-encoded trans-acting factors that interact with the 5' untranslated region (UTR) of these mRNAs. A set of four proteins (60, 55, 47, and 38 kDa) that bind to the 5'-UTR of the psbA mRNA had been identified in C. reinhardtii. 47 kDa protein (RB47) was found to encode a chloroplast poly (A)-binding protein (cPABP) that specifically binds to the 5'-UTR of the psbA mRNA, and essential for translation of this mRNA, cDNA encoding 60 kDa protein (RB60) was isolated, and the amino acid sequence of the encoded protein was highly homologous to plants and mammalian protein disulfide isomerases (PDI), normally found in the endoplasmic reticulum (ER). Immunoblot analysis of C. reinhardtii proteins showed that anti-PDI recognized a distinct protein of 56 kDa in whole cell extract, whereas anti-rRB60 detected a 60 kDa protein. The ER-PDI was not retained on heparin-agarose resin whereas RB60 was retained. In vitro translation products of the RB60 cDNA can be transported into C. reinhardtii chloroplast in vitro. Immunoblot analysis of isolated pea chloroplasts indicated that higher plant also possess a RB60 homolog. In vitro RNA-binding studies showed that RB60 modulates the binding of cPABP to the 5'-UTR of the psbA mRNA by reversibly changing the redox status of cPABP using redox potential or ADP-dependent phosphorylation. Site-directed mutagenesis of -CGHC- catalytic site in thioredoxin-like domain of RB60 is an unique PDI located in the chloroplast of C. reinhardtii, and suggest that the chloroplast PDI may have evolved to utilize the redox-regulated thioredoxin like domain as a mechanism for regulating the light-activated translation of the psbA mRNA.

• Proceedings of the Botanical Society of Korea Conference
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• 1985.08b
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• pp.7-18
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• 1985

Light-regulated translation of chloroplast mRNAs requires nuclear-encoded trans-acting factors that interact with the 5' untranslated region (UTR) of these mRNAs. A set of four proteins (60, 55, 47, and 38 kDa) that bind to the 5'-UTR of the psbA mRNA had been identified in C. reinhardtii. 47 kDa protein (RB47) was found to encode a chloroplast poly (A)-binding protein (cPABP) that specifically binds to the 5'-UTR of the psbA mRNA, and essential for translation of this mRNA, cDNA encoding 60 kDa protein (RB60) was isolated, and the amino acid sequence of the encoded protein was highly homologous to plants and mammalian protein disulfide isomerases (PDI), normally found in the endoplasmic reticulum (ER). Immunoblot analysis of C. reinhardtii proteins showed that anti-PDI recognized a distinct protein of 56 kDa in whole cell extract, whereas anti-rRB60 detected a 60 kDa protein. The ER-PDI was not retained on heparin-agarose resin whereas RB60 was retained. In vitro translation products of the RB60 cDNA can be transported into C. reinhardtii chloroplast in vitro. Immunoblot analysis of isolated pea chloroplasts indicated that higher plant also possess a RB60 homolog. In vitro RNA-binding studies showed that RB60 modulates the binding of cPABP to the 5'-UTR of the psbA mRNA by reversibly changing the redox status of cPABP using redox potential or ADP-dependent phosphorylation. Site-directed mutagenesis of -CGHC- catalytic site in thioredoxin-like domain of RB60 is an unique PDI located in the chloroplast of C. reinhardtii, and suggest that the chloroplast PDI may have evolved to utilize the redox-regulated thioredoxin like domain as a mechanism for regulating the light-activated translation of the psbA mRNA.

• Journal of Korean Ophthalmic Optics Society
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• v.13 no.3
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• pp.13-17
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• 2008

Purpose: The aim of the study is to provide for prospects of Department of Ophthalmic Optics. Methods: This study surveyed on notion in sunder of the frist grade 100 people, second grade 100 people, third grade 100 people in the third educational system of Department of Ophthalmic Optics. Results: 1. Recognition of beforehand information for Department of Ophthalmic Optic were investigated the highest rate at frist grade 40 people(40%) by ordinary, at second grade 42 people(42%) by ordinary and at third grade 34 people(34%) by low. 2. Goodness of fit of register for a course and content for Department of Ophthalmic Optic were investigated the highest rate at frist grade 54 people(54%) by ordinary, at second grade 51 people(51%) and at third grade 47 people(47%) by suitableness. 3. Satisfaction of choice for Department of Ophthalmic Optics were investigated the highest rate at frist grade 43 people(43%) by high, at second grade 45 people(45%) by ordinary and at third grade 41 people(41%) by high. 4. Consideration of instruction for Department of Ophthalmic Optic were investigated the highest rate at frist grade 55 people(55%), at second grade 60 people(60%) and at third grade 49 people (49%) by good. 5. Relationship of friend in Department of Ophthalmic Optics were investigated the highest rate at frist grade 40 people(40%), at second grade 50 people(50%) and at third grade 51 people(51%) by good. 6. Relationship a senior-younger men in Department of Ophthalmic Optics were investigated the highest rate at frist grade 47 people(47%), at second grade 50 people(50%) and at third grade 41 people(41%) by ordinary. 7. Prospects of future for Department Ophthalmic Optics were investigated the highest rate at frist grade 47 people (47%), at second grade 50 people(50%) and at third grade 45 people(45%) by ordinary. 8. Affairs after graduation of Department of Ophthalmic Optics were investigated the highest rate at frist grade 60 people(60%), at second grade 68 people(68%) and at third grade 66 people(66%) by optical shop. Conclusions: Satisfaction of choice for Department and Prospects of future for Department were investigated to be good as a whole.

• Journal of Periodontal and Implant Science
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• v.47 no.3
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• pp.174-181
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• 2017

Purpose: Epitope spreading is a phenomenon in which distinct subdominant epitopes become major targets of the immune response. Heat shock protein (HSP) 60 from Porphyromonas gingivalis (PgHSP60) and peptide 19 from PgHSP60 (Pep19) are immunodominant epitopes in autoimmune disease patients, including those with periodontitis. It remains unclear whether Pep19 is a dominant epitope in subjects without periodontitis or autoimmune disease. The purpose of this study was to determine the epitope spreading pattern and verify Pep19 as an immunodominant epitope in healthy teenagers using dot immunoblot analysis. The patterns of epitope spreading in age-matched patients with type 1 diabetes mellitus (type 1 DM) and healthy 20- to 29-year old subjects were compared with those of healthy teenagers. Methods: Peptide from PgHSP60, Mycobacterium tuberculosis HSP60 (MtHSP60), and Chlamydia pneumoniae HSP60 (CpHSP60) was synthesized for comparative recognition by sera from healthy subjects and patients with autoimmune disease (type 1 DM). Dot immunoblot analysis against a panel of peptides of PgHSP60 and human HSP60 (HuHSP60) was performed to identify epitope spreading, and a densitometric image analysis was conducted. Results: Of the peptide from PgHSP60, MtHSP60, and CpHSP60, PgHSP60 was the predominant epitope and was most consistently recognized by the serum samples of healthy teenagers. Most sera from healthy subjects and patients with type 1 DM reacted more strongly with PgHSP60 and Pep19 than the other peptides. The relative intensity of antibody reactivity to Pep19 was higher in the type 1 DM group than in the healthy groups. Conclusions: Pep19 is an immunodominant epitope, not only in autoimmune disease patients, but also in healthy young subjects, as evidenced by their robust immunoreactivity. This result suggests that the Pep19-specific immune response may be an initiator that triggers autoimmune diseases.

• Food Science of Animal Resources
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• v.39 no.6
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• pp.863-876
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• 2019

The thermal-death times of Listeria monocytogenes were determined in inoculated restructured goat steak at 60℃, 65℃, and 70℃ of sous-vide temperatures. D-values of L. monocytogenes in inoculated restructured goat steak ranged from 7.27 min at 60℃ to 0.46 min at 70℃. Times need to yield at least a 6 log reduction of L. monocytogenes at their temperatures for this product were 47, 12, and 3 min, respectively. After sous-vide, all microbial counts in non-inoculated samples were not detectable, except the aerobic and anaerobic mesophilie and lactic acid bacteria counts were lower than 2 Log CFU/g. For sous-vided and grilled sous-vided samples, sous-vide loss and surface shrinkage were the lowest in samples sous-vided at 60℃ for 47 min (p<0.05). These samples demonstrated the lowest CIE L^*, shear force, hardness, gumminess and chewiness and the highest CIE a^* and hue angle (p<0.05). Therefore, sous-vide at 60℃ for 47 min provided convenient ready-to-cook restructured goat steak for microbiology safety and optimization of physicochemical quality.

• Journal of the Korean Wood Science and Technology
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• v.47 no.2
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• pp.178-188
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• 2019

Licorice, which has an extensive history of use as an herbal medicine, has been suggested to have oral health benefits. However, to date, no systematic study has been conducted on the preparation method of licorice extracts for oral health. In this study, licorice extracts prepared using water and ethanol were investigated for its ability to inhibit the biofilm formation of Streptococcus mutans. The licorice extract prepared with around 60% ethanol effectively inhibited the biofilm formation of S. mutans. Licorice extracted with 50% ethanol almost completely inhibited the biofilm formation at 1.5 g/L of licorice extract. This inhibitory activity was confirmed in a microplate assay and a flow cell system. Glycyrrhetic acid was extracted from licorice effectively with 60% ethanol concentration. The strong inhibitory activity of glycyrrhetic acid and the synergistic inhibition with glycyrrhizin on biofilm formation were suggested as major reasons for a concentration-specific extraction. These results suggest that licorice extract prepared using around 60% ethanol effectively inhibits the biofilm formation of S. mutans.

• Korean Journal of Plant Resources
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• v.22 no.2
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• pp.123-127
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• 2009

This study was carried out to investigate the classification and the growing characteristics of biennial weed species in a newly formed transplanting lawn grass land. Measuring size in the experimental plot was $1{\times}1m$ in the 9 different locations, and experimental design was randomized complete plot with 3 replications. The results obtained are summarized as follows. 1) Weed species in a newly formed lawn field are consisted of 29.4% of Cruciferae, 29.4% of Compositae, 23.5% of Caryophyllaceae, 11.8% of Gramineae, and 5.9% of Labiatae family 2) Emerging percentage of weed species are consisted of 47.1% on October, 41.2% on November, 5.9% on both September and December, respectively. 3) Distribution rate about flowering time of weed species was in order of 58.5% on May, 23.5% on April, 11.8% on March, and 5.9% on June. 4) Weed species were grouped according to the plant height. It was observed that below 20cm, 20-40cm, 40-60cm and above 60cm of plant height were 23.5%, 47.1%, 17.6% and 11.8%, respectively.

• Proceedings of the KSEG Conference
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• 2002.04a
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• pp.47-60
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• 2002

• Journal of the East Asian Society of Dietary Life
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• v.26 no.4
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• pp.334-345
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• 2016

This study conducted surveys on perception and utilization of Korean soybean paste targeting 493 women from their 20s to 60s residing in Daegu. Most subjects married (75.3%), were part of a nuclear family (84.8%), had more than a high school diploma (95.8%), had a career (47.9%), lived in an apartment (68.4%), had a total household income of 5 million won or more (38.1%), and had monthly food expenses more than 0.5 million won and less than 1 million won (42.2%). Regarding perception, 'rich nutrition' was the most commonly held perception in subjects in their 60s (4.4), followed by 'good taste' in subjects in their 40s (4.3). Regarding reasons for consumption 'healthy' was highest in subjects in their 60s (47.3%) and 40s (45.6%) and 'delicious' in subjects in their 20s (56.1%). Regarding how to make it, subjects in their 60s responded 'know for sure' (57.1%), while only 2% of 20s responded the same. Subjects in their 50s and 60s had a higher ratio of answering 'know' while 20s and 30s 'don't know', For preparation methods, subjects in their 20s (53.1%) and 30s (41.4%) answered 'buy at the market', subjects in their 40s (68.9%) 'get from family or relatives' and subjects in their 60s (47.3%) 'make by one's own'. For preservation method, subjects in their 20s and 30s answered 'preserve in refrigerator', the 60s tend to answer 'preserve in jangdokdae'. Buying at the market was most common in subjects in their 20s (36.1%) and 30s (28.5%) and the reason was 'convenient' (39.6%). The place of purchase was 'in large supermarket or department store' (78.5%). For the future consumption pattern, 'increase in consumption of commercialized product (44.0%)', subjects in their 60s answered 'keep making at home' while subjects in their 30s and 40s answered 'keep making at home or buying product half and half'. According to the above study results, women living in Daegu region were aware of excellence of traditional soybean paste. However, due to changes in residential circumstance and lack of knowledge about the recipe, they did not make. They receive soybean paste t from family, relatives or bought in the market, which indicates the continuous decrease in home-made soybean paste. Therefore, due to the expanding social participation of women, the product market is expected to increase. I think it is important to develop traditional commercial soybean paste in the traditional way to improve the traditional flavors.

• Journal of the Korean earth science society
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• v.18 no.6
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• pp.559-564
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• 1997

The New Hebrides Basin is an inactive non back-arc basin located at the convergent boundary of the Pacific and Info-Australian plates. This basin was formed from 46 Ma to 60 Ma. The basin has two spreading episodes with rates of 34 mm/a for 42 to 47 Ma and 17 mm/a for 47 to 60 Ma. The sediments covered in the basin has uniform thickness of 0.65 sec. The age-depth correlation curve of the New Hebrides Basin can be represented by the following equation: $Depth(m)=2689+312\sqrt{Age}(Ma)$ The coefficient of 312 in this equation is close to that for major oceans, 350. This suggests that the cooling processes of the lithospheres in the New Hebrides Basin and major oceans are similar to each other. Free-air gravity anomalies of the basin varying from -22.3 mgal to +59.0 mgal. The mean value is +30.2 mgal higher than those of the normal oceans. Moderately large free-air gravity anomalies in the New Hebrides Basin are presumably owing to its location on a marginal swell along the New Hebrides Trench. It is generally observed that the ocean floor is very gently uplifted in a zone about 200 km oceanward of the trench axis. Positive free-air gravity anomalies amounting to $50{\sim}60$ mgal are usually observed on the crest of the swell. This topography is presumably by bending of the oceanic lithosphere so as to dynamically maintain nonisostatic states for some duration.