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EVALUATION OF FLUORIDE-RELEASING CAPACITY FROM POLYVINYL ALCOHOL POLYMER TAPE SUPPLEMENTED WITH NAF IN ORAL CAVITY (폴리비닐알코올 기반 고분자 불소 함유 테이프의 구강 내 불소 유리 성능 평가)

  • Lee, Ka-Young;Lee, Sang-Ho;Lee, Nan-Young
    • Journal of the korean academy of Pediatric Dentistry
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    • v.40 no.2
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    • pp.89-97
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    • 2013
  • The purpose of this study is to investigate fluoride release in the oral cavity from polymer adhesive tape which is NaF coated PVA. 45 healthy adults were divided into 3 groups by the type of topical fluoride applied: 60seconds taste$^{(R)}$ APF gel (group 1), FluoroDose$^{(R)}$ varnish (group 2) and NaF-PVA (group 3). Topical fluoride was applied to the facial surface of maxillary 12 teeth and unstimulated whole saliva was collected to measure fluoride release after 1, 3, 6, 12, 24, and 48 hours. Fluoride-sensitive electrode was used for measuring the fluoride concentration in the saliva samples. All three groups showed significantly higher value for fluoride concentration than the baseline after 1 and 3 hours (p < 0.05). After 6 hours, group 3 showed significantly higher fluoride concentration than the baseline (p < 0.05) and also showed significantly higher value for fluoride concentration than group 1 and group 2. Between group 1 and group 2, however, there was no significant difference statistically with respect to fluoride concentration value (p > 0.05).

Changes in fermentation pattern and quality of Italian ryegrass (Lolium multiflorum Lam.) silage by wilting and inoculant treatments

  • Liu, Chang;Zhao, Guo Qiang;Wei, Sheng Nan;Kim, Hak Jin;Li, Yan Fen;Kim, Jong Geun
    • Animal Bioscience
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    • v.34 no.1
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    • pp.48-55
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    • 2021
  • Objective: This study was conducted to investigate the effects of wilting and microbial inoculant treatment on the fermentation pattern and quality of Italian ryegrass silage. Methods: Italian ryegrass was harvested at heading stage and ensiled into vinyl bags (20 cm×30 cm) for 60d. Italian ryegrass was ensiled with 4 treatments (NWNA, no-wilting noadditive; NWA, no-wilting with additive; WNA, wilting no-additive; WA, wilting with additive) in 3 replications, wilting time was 5 hours and additives were treated with 106 cfu/g of Lactobacillus plantarum. The silages samples were collected at 1, 2, 3, 5, 10, 20, 30, 45, and 60 days after ensiling and analyzed for the ensiling quality and characteristics of fermentation patterns. Results: Wilting treatment resulted in lower crude protein and in vitro dry matter digestibility and there were no significant differences in acid detergent fiber (ADF), total digestible nutrient (TDN), water-soluble carbohydrate (WSC), ammonia content, and pH (p>0.05). However, wilting treatment resulted in higher ADF and neutral detergent fiber content of Italian ryegrass silage (p<0.05), and the WNA treatment showed the lowest TDN and in vitro dry matter digestibility. The pH of the silage was higher in the wilting group (WNA and WA) and lower in the additive treatment group. Meanwhile, the decrease in pH occurred sharply between the 3-5th day of storage. The ammonia nitrogen content was significantly lower in the additive treatment (p<0.05), and wilting had no effect. As fermentation progressed, the lactic and acetic acid contents were increased and showed the highest content at 30 days of storage. Conclusion: The wilting treatment did not significantly improve the silage fermentation, but the inoculant treatment improved the fermentation patterns and quality of the silage. So, inoculation before ensiling is recommended when preparing high quality of Italian ryegrass silage, and when wilting, it is recommended to combine inoculation for making high quality silage.

Effects of protein content and the inclusion of protein sources with different amino acid release dynamics on the nitrogen utilization of weaned piglets

  • Hu, Nianzhi;Shen, Zhiwen;Pan, Li;Qin, Guixin;Zhao, Yuan;Bao, Nan
    • Animal Bioscience
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    • v.35 no.2
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    • pp.260-271
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    • 2022
  • Objective: We aimed to investigate the effect of the differing amino acid (AA) release dynamics of two protein sources on the growth performance, nitrogen deposition, plasma biochemical parameters, and muscle synthesis and degradation of piglets when included in their diets at normal and low concentrations. Methods: Forty-eight piglets (Duroc×Landrace×Large White) with initial body weight of 7.45±0.58 kg were assigned to six groups and fed one of 6 diets. The 6 dietary treatments were arranged by 3×2 factorial with 3 protein sources and 2 dietary protein levels. They are NCAS (a normal protein content with casein), NBlend (a normal protein content with blend of casein and corn gluten meal), NCGM (a normal protein content with corn gluten meal), LCAS (a low protein content with casein), LBlend (a low protein content with blend of casein and corn gluten meal), LCGM (a low protein content with corn gluten meal). The release dynamics of AA in these diets were determined by in vitro digestion. The digestibility, utilization and biological value of nitrogen in piglets were determined by micro Kjeldahl method. Plasma insulin was measured by enzyme-linked immunosorbent assay kits. The protein expression of mediators of muscle synthesis and degradation was determined by western blotting. Results: Although the consumption of a low-protein diet supplemented with crystalline AA was associated with greater nitrogen digestion and utilization (p<0.05), the final body weight, growth performance, nitrogen deposition, and phosphorylation of ribosomal protein S6 kinase 1 and eIF4E binding protein 1 in the muscle of pigs in the low-protein diet-fed groups were lower than those of the normal-protein diet-fed groups (p<0.05) because of the absence of non-essential AA. Because of the more balanced release of AA, the casein (CAS) and Blend-fed groups showed superior growth performance, final body weight and nitrogen deposition, and lower expression of muscle ring finger 1 and muscle atrophy F-box than the CGM-fed groups (p<0.05). Conclusion: We conclude that the balanced release of AA from CAS containing diets and mixed diets could reduce muscle degradation, favor nitrogen retention, % intake and improve growth performance in pigs consuming either a normal- or low-protein diet.

Distribution characteristics of Manchurian and China-Japan-Korea flora in Korean Peninsula

  • Kim, Nam Shin;Lim, Chi Hong;Cha, Jin Yeol;Cho, Yong Chan;Jung, Song Hie;Jin, Shi Zhu;Nan, Ying
    • Journal of Ecology and Environment
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    • v.46 no.3
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    • pp.259-272
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    • 2022
  • Background: The Korean Peninsula exhibits a characteristic graded floral distribution, with northern (Manchurian flora) and southern (China-Japan-Korea flora) lineage species coexisting according to climatic and topographical characteristics. However, this distribution has been altered by climate change. To identify ecosystem changes caused by climate change and develop appropriate measures, the current ecological status of the entire Korean Peninsula should first be determined; however, analysis of the current floral distribution in North Korea has been hampered for political reasons. To overcome these limitations, this study constructed a database of floral distributions in both South and North Korea by integrating spatial information from the previously established National Ecological Survey in South Korea and geocoding data from the literature on biological distributions published in North Korea. It was then applied to analyze the current status and distribution characteristics of Manchurian and China-Japan-Korea plant species on the Korean Peninsula. Results: In total, 45,877 cases were included in the Manchurian and China-Japan-Korea floral distribution database. China-Japan-Korea species were densely distributed on Jeju-do and along the southern coast of the Korean Peninsula. The distribution density decreased as the latitude increased, and the distributions reached higher-latitude regions in the coastal areas compared with the inland regions. Manchurian species were distributed throughout North Korea, while they were densely distributed in the refugia formed in the high-elevation mountain regions and the Baekdudaegan in South Korea. In the current distribution of biomes classified according to the Whittaker method, subtropical and endemic species were densely distributed in temperate seasonal forest and woodland/shrubland biomes, whereas boreal species were densely distributed in the boreal forest biome Korean Peninsula, with a characteristic gradation of certain species distributed in the temperate seasonal forest biome. Factor analysis showed that temperature and latitude were the main factors influencing the distribution of flora on the Korean Peninsula. Conclusions: The findings reported herein on the current floral distribution trends across the entire Korean Peninsula will prove valuable got mitigating the ecological disturbances caused by ongoing climate change. Additionally, the gathered flora data will serve as a basis for various follow-up studies on climate change.

Evaluation of forage production, feed value, and ensilability of proso millet (Panicum miliaceum L.)

  • Wei, Sheng Nan;Jeong, Eun Chan;Li, Yan Fen;Kim, Hak Jin;Ahmadi, Farhad;Kim, Jong Geun
    • Journal of Animal Science and Technology
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    • v.64 no.1
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    • pp.38-51
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    • 2022
  • Whole-plant corn (Zea may L.) and sorghum-sudangrass hybrid [Sorghum bicolor (L.) Moench] are major summer crops that can be fed as direct-cut or silage. Proso millet is a short-season growing crop with distinct agronomic characteristics that can be productive in marginal lands. However, information is limited about the potential production, feed value, and ensilability of proso millet forage. We evaluated proso millet as a silage crop in comparison with conventional silage crops. Proso millet was sown on June 8 and harvested on September 5 at soft-dough stage. Corn and sorghum-sudangrass hybrid were planted on May 10 and harvested on September 10 at the half milk-line and soft-dough stages, respectively. The fermentation was evaluated at 1, 2, 3, 5, 10, 15, 20, 30, and 45 days after ensiling. Although forage yield of proso millet was lower than corn and sorghum-sudangrass hybrid, its relative feed value was greater than sorghum-sudangrass hybrid. Concentrations of dry matter (DM), crude protein, and water-soluble carbohydrate decreased commonly in the ensiling forage crops. The DM loss was greater in proso millet than those in corn and sorghum-sudangrass hybrid. The in vitro dry matter digestibility declined in the forage crops as fermentation progressed. In the early stages of fermentation, pH dropped rapidly, which was stabilized in the later stages. Compared to corn and sorghum-sudangrass hybrid, the concentration of ammonia-nitrogen was greater in proso millet. The count of lactic acid bacteria reached the maximum level on day 10, with the values of 6.96, 7.77, and 6.95 Log10 CFU/g fresh weight for proso millet, corn, and sorghum-sudangrass hybrid, respectively. As ensiling progressed, the concentrations of lactic acid and acetic acid of the three crops increased and lactic acid proportion became higher in the order of sorghum-sudangrass hybrid, corn, and proso millet. Overall, the shorter, fast-growing proso millet comparing with corn and sorghum-sudangrass hybrid makes this forage crop an alternative option, particularly in areas where agricultural inputs are limited. However, additional research is needed to evaluate the efficacy of viable strategies such as chemical additives or microbial inoculants to minimize ammonia-nitrogen formation and DM loss during ensiling.

Job Analysis for Role Identification of Clinical Nurses with Expanded Role - Clinical Nurse with Education and Clinical Nurse with Coordination - (진료협력간호사의 역할규명을 위한 직무분석 - 교육상담간호사와 진료코디네이터간호사 중심 -)

  • Sung, Young Hee;Lim, Nan Young;Park, Kwang Ok;Jung, Jung Hee;Kwon, In Gak;Kim, Ul Soon;Hwang, Moon Sook
    • Journal of Korean Clinical Nursing Research
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    • v.15 no.2
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    • pp.139-155
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    • 2009
  • Purpose: The purpose of this study was to identify role through job analysis (duties and tasks) of nurses who work as clinical nurses with education and clinical nurse with patient coordination within the hospital. Methods: The DACUM committee was organized with 8 nurses each, clinical nurses with education and clinical nurses with coordination. The committee derived the duties and tasks of the two groups of clinical nurses from their actual work in the hospital through a DACUM workshop. Validity of the derived duties and tasks was tested by 12 nurses at each of 3 hospital. Results: For clinical nurse with education, 8 duties and 45 tasks were identified, which included patient education and consultation, patient advocacy, management of the therapeutic process, direct nursing care, activities work directed at improvement, management of health promotion events, administration work, and self-improvement. For clinical nurse with coordination, 10 duties and 54 tasks were identified, and included client consultation, client education, direct nursing care, coordination of therapeutic flow, employee education, public relations with the community, planning and operation of meetings, administration work, work directed at improvement, and self-improvement. Conclusion: The results of this study contribute to not only the work of the participants but also basic data for human resource management in the hospital.

Development and Validation of a Prognostic Nomogram Based on Clinical and CT Features for Adverse Outcome Prediction in Patients with COVID-19

  • Yingyan Zheng;Anling Xiao;Xiangrong Yu;Yajing Zhao;Yiping Lu;Xuanxuan Li;Nan Mei;Dejun She;Dongdong Wang;Daoying Geng;Bo Yin
    • Korean Journal of Radiology
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    • v.21 no.8
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    • pp.1007-1017
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    • 2020
  • Objective: The purpose of our study was to investigate the predictive abilities of clinical and computed tomography (CT) features for outcome prediction in patients with coronavirus disease (COVID-19). Materials and Methods: The clinical and CT data of 238 patients with laboratory-confirmed COVID-19 in our two hospitals were retrospectively analyzed. One hundred sixty-six patients (103 males; age 43.8 ± 12.3 years) were allocated in the training cohort and 72 patients (38 males; age 45.1 ± 15.8 years) from another independent hospital were assigned in the validation cohort. The primary composite endpoint was admission to an intensive care unit, use of mechanical ventilation, or death. Univariate and multivariate Cox proportional hazard analyses were performed to identify independent predictors. A nomogram was constructed based on the combination of clinical and CT features, and its prognostic performance was externally tested in the validation group. The predictive value of the combined model was compared with models built on the clinical and radiological attributes alone. Results: Overall, 35 infected patients (21.1%) in the training cohort and 10 patients (13.9%) in the validation cohort experienced adverse outcomes. Underlying comorbidity (hazard ratio [HR], 3.35; 95% confidence interval [CI], 1.67-6.71; p < 0.001), lymphocyte count (HR, 0.12; 95% CI, 0.04-0.38; p < 0.001) and crazy-paving sign (HR, 2.15; 95% CI, 1.03-4.48; p = 0.042) were the independent factors. The nomogram displayed a concordance index (C-index) of 0.82 (95% CI, 0.76-0.88), and its prognostic value was confirmed in the validation cohort with a C-index of 0.89 (95% CI, 0.82-0.96). The combined model provided the best performance over the clinical or radiological model (p < 0.050). Conclusion: Underlying comorbidity, lymphocyte count and crazy-paving sign were independent predictors of adverse outcomes. The prognostic nomogram based on the combination of clinical and CT features could be a useful tool for predicting adverse outcomes of patients with COVID-19.

Screening of Antifungal Activity on the Coastal Plants 5 Species (해안식물 5종에 대한 항균활성 탐색)

  • Kwon, Nan-Hee;Kim, Tae-Keun;Park, Sung-Jun;Kim, Hyoun-Chol;Song, Chang-Khil
    • Korean Journal of Organic Agriculture
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    • v.24 no.3
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    • pp.465-484
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    • 2016
  • This study evaluated the antifungal activity of varying concentrations of water-soluble extracts from native plants (Vitex rotundifolia, Tetragonia tetragonoides, Artemisia capillaris, Hibiscus hamabo and Ficus carica) against Stemphylium vesicarium, Penicillium italicum, Sclerotinia sclerotiorum, Pythium ultimum, Botrytis cinerea, Rhizoctonia solani and Colletotrichum gloeosporioides. Mycelium growth of pathogenic bacteria generally decreased in a concentration-dependent manner following treatment with the water extracts from donor plants. Closer analyses indicate varying inhibitory capacities depending on the type of donor plant and pathogenic bacteria. Specifically, mycelium growth of S. vesicarium varied depending on the concentration of the water extracts from T. tetragonoides (r = -0.857, p<0.01) and A. capillarys (r = -0.868, p<0.01). Also, P. italicum and V. rotundifolia (r = -0.833, p<0.01), S. sclerotiorum and V. rotundifolia (r = -0.862, p<0.01), A. capillaris (r = -0.902, p<0.01), B. cinerea and T. tetragonoides (r = -0.896, p<0.01) showed an inverse relationship. The rate of mycelial growth inhibition of pathogenic bacteria analysed are as follows: P. ultimum 94%, B. cinerea 50%, C. gloeosporioides 80% in 100% treatment of T. teragonoides. A. capillaris inhibited S. vesicarium by 43%, P. ultimum by 90%; H. hamabo inhibited P. italicum by 50%, S. sclerotiorum by 26%, and F. carica inhibited R. solani by 74%. Total phenol content with antifungal activities are as follows: A. capillaris 16.15 mg/g, F. carica 7.81 mg/g, V. rotundifolia 6.18 mg/g, H. hamabo 5.25 mg/g, T. tetragonoides 4.41 mg/g, and total flavonoid content is as follows: A. capillaris 27.57 mg/g, V. rotundifolia 12.49 mg/g, F. carica 11.45 mg/g, H. hamabo 5.77 mg/g, T. tetragonoides 5.08 mg/g.

Probabilistic Exposure Assessment of Pesticide Residues in Agricultural Products in Gyeonggi-do (경기도내 유통 농산물 중 잔류농약의 확률론적 노출평가 연구)

  • Do, Young-Sook;Kim, Jung-Boem;Kang, Suk-Ho;Kim, Nan-Young;Eom, Mi-Na;Yoon, Mi-Hye
    • The Korean Journal of Pesticide Science
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    • v.17 no.2
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    • pp.117-125
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    • 2013
  • A probabilistic exposure assessment was performed on the monitoring data of pesticides were assessed in agricultural products in Gyeonggi-do from 2006 to 2010. Chlorothalonil, chlorpyrifos, dicofol, endosulfan, EPN, ethoprophos, fenitrothion, methidathion, phenthoate and tebupirimfos were assessed. For this assessment, we used Monte Carlo simulation software and the distribution of concentration and intake were assumed to lognormal distribution by inputting mean and standard deviation. The hazard index (HI, %ADI) of average value and the $95^{th}$ percentile based on a probabilistic method were usually lower than those by a deterministic one. For the whole population, when non-detects data were assigned 0 mg/kg, HI of the average value and the $95^{th}$ percentile showed 0.05~0.70% and 0.11~1.94%, respectively. When nondetects data were assigned 0.005 mg/kg, HI of the average value and the $95^{th}$ percentile were 0.41~4.42% and 0.98~13.81%. For only consumers, when non-detects data were assigned 0 mg/kg, HI of the average value and the $95^{th}$ percentile were 1.24~10.16% and 3.72~33.81%, respectively. When non-detects data were assigned 0.005 mg/kg, HI of the average value and the $95^{th}$ percentile were 3.43~18.26% and 9.45~54.99%, respectively. Methidathion had highest values when both of 0 and 0.005 were assigned to non-detecs data for consumers only. This study showed that agricultural products in Gyeonggi-do were safe because they had less than 100 of HI (%ADI) based on probabilistic exposure assessment.

Incorporation Effect of Green Manure Crops on Improvement of Soil Environment on Saemangeum Reclaimed Land during Sorghum×Sudangrass Hybrid Cultivation (수수×수단그라스 재배시 녹비작물 혼입에 따른 새만금간척지 토양환경 개선 효과)

  • Yang, Chang-Hyu;Lee, Jang-Hee;Baek, Nan-Hyun;Jeong, Jae-Hyeok;Cho, Kwang-Min;Lee, Sang-Bok;Lee, Gyeong-Bo
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.5
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    • pp.744-748
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    • 2012
  • This study was carried out to investigate the incorporation effect of green manure crops (GMC) such as the hairy vetch on improvement of soil environment in reclaimed land during sorghum${\times}$sudangrass hybrid (SSH) cultivation over the past three years from 2009 to 2011. Plots consisted of conventional fertilization (CF) and incorporation of GMC were divided by rates of additional nitrogen fertilizer ($100kg\;ha^{-1}$) and decreased percentage of 30 50 70 100 fertilization in addition to non nitrogen fertilization (NNF). Soil physico-chemical properties, growth and yield potential were examined. The results were as follows. The testing soil was showed strong alkaline saline soil with low organic matter contents and less available phosphate while exchangeable sodium and magnesium were higher. Soil salinity was increased during cultivation of summer crop. However, SSH was not affected by salt content. The fresh weight of GMC at incorporation time was $18,345kg\;ha^{-1}$. Content of total nitrogen at incorporation time was 3.09% and the C/N ratio was 12.8. Fresh and dry matter yield of SSH were higher in the order of 30%, CF, N50%, N70%d, N100%, and NNF. Fresh and dry matter yield of SSH increased in the order of CF ($55,050kg\;ha^{-1}$, $16,250kg\;ha^{-1}$), N contents from 30% to 9%. Soil physical properties, such as bulk density were decrease with incoporation of GMC, while porosity was increased. Soil chemical properties, such as pH was decreased while content of exchangeable calcium, available phosphate, and organic matter were increased. Also contents of exchangeable sodium and potassium were decreased with incorporation of GMC than those before experiment. Thus, we assumed that incorporation of hairy vetch was more effective that can lead to reduce chemical nitrogen fertilizer and to improve soil environment in cultivating SSH on Saemangeum reclaimed land.