• Title/Summary/Keyword: 400 mH

Search Result 937, Processing Time 0.027 seconds

Characterization of Extracellular Peroxidase from Pleurotus ostreatus (Pleurotus ostreatus에서 분비되는 Peroxidase의 특성)

  • 배성호;신광수;강사욱;하영칠;최선진;김규중;최형태
    • Korean Journal of Microbiology
    • /
    • v.27 no.4
    • /
    • pp.348-356
    • /
    • 1989
  • An extracellular peroxidase found in culture broth of Pleurotus ostreatus was induced by syringic acid. This enzyme was fractionated by DEAE Sephadex A-50 ion exchange chromatography and gel filtration chromatogrphy on Sephadex G-150. The enzyme is a glycoprotein containing 35.7% carbohydrate. The results of SDS-linear polyacrylamide gradient gel electrophoresis and gel filtration indicate that the enzyme is a dimer consisted of identical subunits (Mr=72,400). The absorption spectrum of the enzyme indicates the presence of one mole of iron protoporphyrin IX per one mole of subunit. Isoelectric point of the enzyme is 4.26 and $K_m$ values for $H_2O_2$ is $7.2{\mu}M$. The enzyme showed its optimal activity at pH 3.5-4.0 and at $40^{\circ}C$. The Km values of this enzyme for ferulic acid and sinapic acid are 2.4 and 12.3 times higher than those of horseradish peroxidase, respectively.

  • PDF

$CH_4N_2S$$C_{10}H_{13}NO_3S$ 첨가가 Ni 패턴 상의 구리도금 형상에 미치는 영향

  • Lee, Jin-Hyeong;Lee, Ju-Yeol;Kim, Man
    • Proceedings of the Korean Institute of Surface Engineering Conference
    • /
    • 2009.10a
    • /
    • pp.155-155
    • /
    • 2009
  • The copper plating was deposited by pulse reverse current (PRC) method with additives. The all specimens were first immersted in 10% H2SO4 for 10 minutes, and then were rinsed with deionized water. The current densities of forward pulse were 400mA/$cm^2$, and those of reverse pulse were 1900mA/$cm^2$ and 100mA/$cm^2$. Results are compared for different additives for pulse plating conditions. When it added in Only CH4N2S (TU) or only C10H13NO3S (SVH), the effect of surface side growth of Cu was not different. But when it added in TU and SVH, surface side growth of Cu decreased. Polarization curves were measured from OCP to -0.7 V at a rate of 1mV/sec. Each specimen was observed under the PHENOM to see surface morphology.

  • PDF

Biosorption of Cobalt by Methanotrophic Biomass (메탄자화균에 의한 코발트의 생물흡착)

  • Lee, Moo-Yeal;Yang, Ji-Won
    • Journal of Korean Society of Environmental Engineers
    • /
    • v.22 no.12
    • /
    • pp.2163-2173
    • /
    • 2000
  • The optimum pH range for biosorption of cobalt by methanotrophic bacteria was broadened to 6.0~12.0 which was compared to pH 10.5~11.5 of bios or bent-free control case. Removal efficiency of cobalt by methanotrophic biomass was pH dependent, but less sensitive than that of control. With 1.0 g biosorbent/L at initial solution pH 6.0. methanotrophic biomass took up cobalt from aqueous solutions to the extent of 170 mg/g biomass. As a result of scanning electron microscope(SEM) micrographs, cobalt removal by methanotrophic biomass seemed to be through adsorption on the surface of methanotrophic biomass and by exopolymer around the biomass. Optimum amount of methanotrophic biomass for maximum cobalt uptake was 1.0 g/L for initial 400 mg Co/L at initial pH 6.0. Removal efficiency of cobalt was slightly affected by ionic strength up to 2.0 M of NaCl and $NaNO_3$, respectively.

  • PDF

A study on the characteristics of planar type inductively coupled plasma and its applications on the selective oxide etching (평면형 유도결합 플라즈마의 특성 및 선택적 산화막 식각 응용에 관한 연구)

  • 양일동;이호준;황기웅
    • Journal of the Korean Vacuum Society
    • /
    • v.6 no.1
    • /
    • pp.91-96
    • /
    • 1997
  • The electrical characteristics and the plasma parameters of planar inductively coupled plasmas (ICP) have been measured. The resistance of the total load including the coil and the plasma varied from 1 to 4 W and the inductance from 1.5 m to 2 mH when the power was changed from 100 to 1000 W and the pressure from 1 to 10 mTorr. The density of electron measured by Langmuir probe was over $10^{11}/\textrm{cm}^3$ and the temperature varied between 3 and 5 eV as the process conditions were changed. Bias modulation was adopted as a new method to improve the selectivity of $SiO_2$on Si in $C_4F_8$ (octafluorocyclobutane) plasma. The selectivity was improved as the duty ratio decreased, but the etch rate of $SiO_2$decreased below 400$\AA$/min. $H_2$addition to $C_4F_8$ plasma showed that the etch selectivity could be higher than 50 and the etch rate of $SiO_2$over 2000$\AA$/min when 60% $H_2$was added.

  • PDF

Floristic Composition and Vertical Distribution of Mt. Daesun (대둔산 식물의 분류학적 특성과 수직분포)

  • Cho, Joong Bae;Bong-Seop Kil
    • The Korean Journal of Ecology
    • /
    • v.10 no.2
    • /
    • pp.53-62
    • /
    • 1987
  • Floristic composition and vertical distribution of Mt. Daedun (western slope) were investigated from 1985 to 1986. The results are as follows; 108 families, 346 genera, 511 species, 2 subspecies, 75 varieties, 5 forma or 593 taxa including 77 cultivars. The floral data showed the ecological characteristics such as the value 346 in Fisher's Index, H-D1-H5 in biological type, 10.9% in urban index of naturalized plants and 60.5% in erect form growth form. Based on the physiognomy and population density of dominant tree species the forest vegetation of Mt. Daedun (western slope) was classified into 5 types; Quercus mongolica forest and Q. variabilis forest at 800m above, Carpinys laxiflora forest and Acer mono forest at 700m to 800m and Sapium japonicum forest at 500m to 800m in altitude. And Lindera erythrocarpa forest and zelkova serrata forest are widely distributed at 400m to 800m in altityde along valley and at mountain foot area.

  • PDF

An Application of the Water Mist System for Underground Utility Tunnel (지하구 미분무수 소화설비 적용에 관한 연구)

  • 김운형;김종훈;박승민;김태수;민인홍;전동일;김상욱
    • Fire Science and Engineering
    • /
    • v.16 no.1
    • /
    • pp.66-76
    • /
    • 2002
  • This paper includes new nozzle design, basic design factors of water mist system that minimize a thermal damage of cable causing business interruption and applying underground utility tunnel. A underground concrete structure (2.5 m(H)$\times$2.5 m(W)$\times$25 m(D)) is constructed in order to test a nozzle performance. Under the designing fire scenario, critical thermal damage of cable sheath ($400^{\circ}c$) reached within a 2 minutes with unsuppressed fire, but type 1 nozzle (SMD 470 $\mu{m}$) and type 2 nozzle (SMD 650 $\mu{m}$) control cable temperature below $400^{\circ}c$. A system performance and fundamental design factors; K factor, flow rate, spray angle, size distribution, nozzle pressure, spray density are analyzed and proposed for system optimization.

Identification of Novel Saringosteryl Glucoside in Phaseolus vulgaris Seed (강낭콩 미숙종자내 신규 Saringosteryl Glucoside의 동정)

  • 김성기
    • Journal of Plant Biology
    • /
    • v.37 no.4
    • /
    • pp.441-444
    • /
    • 1994
  • From immature seed of Phaseolus vulgaris L., a novel phytosteryl glucoside was isolated. Strong ion peaks at m/z 613 $[M+Na}^{+},\;696\;[M+Matrix]^{+}$ in positive F AB- MS and at m/z 589 $[M-1]^{-}$ in negative F AB- MS indicated the molecular weight of the compound is 590. Four hundred MHz $^IH-NMR$ analysis revealed that the compound canys a 24-hydroxy-24-vinyl-cholesterol (saringosterol) as an aglycone and a ${\beta}-D-glucopyranose$. Four hundred MHz $^IH-NMR$ analysis of the acetate derivate of the compound revealed that hydroxyls at C-1' in glucose moeity and at C-3 in aglycone have been condensed. Therefore, the phytosteryl glucoside was characterized to be $3-0-{\beta}-D-glucopyranosyl-24-hydroxy-24-vinyl-cholesterol$ (saringosteryl glucoside). This is the first demonstration for the presence of saringosterol in higher plants. Also this is the first identification of saringosteryl glucoside in natural materials.erials.

  • PDF

Electrochemical Characteristics of Lithium Battery Anode Materials Using Petroleum Pitches (석유계 피치를 사용한 리튬전지 음극소재의 전기화학적 특성)

  • Hwang, Jin Woong;Lee, Jong Dae
    • Applied Chemistry for Engineering
    • /
    • v.28 no.5
    • /
    • pp.534-538
    • /
    • 2017
  • In this study, the molecular weight controlled pitches derived from pyrolyzed fuel oil (PFO) were prepared using solvent extraction and were carbonized. Electrochemical characteristics of lithium battery anode materials were investigated using these petroleum pitches. Three pitch samples prepared by the thermal reaction were 3903 (at $390^{\circ}C$ for 3 h), 4001 (at $400^{\circ}C$ for 1 h) and 4002 (at $400^{\circ}C$ for 2 h). The prepared hexane insoluble pitches were analysed by XRD, TGA, SEM and Gel permeation Chromatography (GPC). The electrochemical characteristics of the PFO-derived pitch as an anode material were investigated by constant current charge/discharge, cyclic voltammetry and electrochemical impedance tests. The coin cell using pitch (4001) and the electrolyte of $LiPF_6$ in organic solvents (EC : DMC = 1 : 1 vol%, VC 3 wt%) has better initial capacity (310 mAh/g) than that of other pitch coin cells. Also, this carbon anode showd a high initial efficiency of 82%, retention rate capability at 2 C/0.1 C of 90% and cycle retention of 85%. It was found that modified pitches improved the cycling and rate capacity performance.

Effect of Dietary Cation-Anion Difference during Prepartum and Postpartum Periods on Performance, Blood and Urine Minerals Status of Holstein Dairy Cow

  • Razzaghi, A.;Aliarabi, H.;Tabatabaei, M.M.;Saki, A.A.;Valizadeh, R.;Zamani, P.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.25 no.4
    • /
    • pp.486-495
    • /
    • 2012
  • Twenty four periparturient cows were used to determine the effects of DCAD on acid-base balance, plasma and urine mineral concentrations, health status, and subsequent lactation performance. Each group of 12 cows received either a diet containing -100 DCAD or +100 DCAD for 21 d prepartum. Both anionic and cationic groups were divided into two groups, one received a +200 DCAD and the other +400 DCAD diet for 60 d postpartum. Prepartum reduction of DCAD decreased DMI, urinary and blood pH, urinary concentrations of Na or K and increased plasma and urinary Ca, Mg, Cl and S. Also cows fed -100 DCAD diet consumed the most dry matter in the first 60 d after calving. Postpartum +400 DCAD increased milk fat and total solid percentages, urinary and blood pH and urinary Na and K concentrations, but urinary Ca, P, Cl and S contents decreased. Greater DMI, FCM yields were observed in cows fed a diet of +400 DCAD than +200 DCAD. No case of milk fever occurred for any diets but feeding with a negative DCAD diet reduced placenta expulsion time. In conclusion, feeding negative DCAD in late gestation period and high DCAD in early lactation improves performance and productivity of dairy cows.

A Study on Noise Propagation Properties of Hi-Speed Train (고속으로 운행하는 고속철도의 소음 방사특성 연구)

  • Park, Tae-Ho;Han, Jong-Won;Ryu, Hun-Jae;Ko, Jun-Hee;Chang, Seo-Il
    • Proceedings of the KSR Conference
    • /
    • 2011.10a
    • /
    • pp.2934-2940
    • /
    • 2011
  • The railway system in Korea is developing more and more rapidly. After development the next generation hi-speed train(HEMU-400X), Korea can compete against developed country. But, speed increasing will cause the greater rolling and aero dynamic noise. In this study shows that measurement noise of the KTX train by 300kn/h class, and KTX-Sancheon train by 350km/h class. The measurements are carried out on open area and noise protection barrier installed area. The microphones were placed at a distance of 3m from the center line of the track at a height of 0.5m above the upper surface of the rail and at a distance of 7.5m from the center line of the track at a height of each 0.6m above(Max 3.6m) the upper surface of the rail. One microphone was placed at a distance of 25m for check the environmental noise.

  • PDF