• The Korean Journal of Physiology and Pharmacology
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• v.21 no.1
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• pp.133-140
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• 2017

Conflicting evidence has been obtained regarding whether transient receptor potential cation channels (TRPC) are store-operated channels (SOCs) or receptor-operated channels (ROCs). Moreover, the Ca/Na permeability ratio differs depending on whether the current-voltage (I-V) curve has a doubly rectifying shape or inward rectifying shape. To investigate the calcium permeability of TRPC4 channels, we attached GCaMP6s to TRPC4 and simultaneously measured the current and calcium signals. A TRPC4 specific activator, (-)-englerin A, induced both current and calcium fluorescence with the similar time course. Muscarinic receptor stimulator, carbachol, also induced both current and calcium fluorescence with the similar time course. By forming heteromers with TRPC4, TRPC1 significantly reduced the inward current with outward rectifying I-V curve, which also caused the decrease of calcium fluorescence intensity. These results suggest that GCaMP6s attached to TRPC4 can detect slight calcium changes near TRPC4 channels. Consequently, TRPC4-GCaMP6s can be a useful tool for testing the calcium permeability of TRPC4 channels.

• ALGAE
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• v.36 no.4
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• pp.315-326
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• 2021

Ion channels are membrane protein complexes mediating passive ion flux across the cell membranes. Every organism has a certain set of ion channels that define its physiology. Dinoflagellates are ecologically important microorganisms characterized by effective physiological adaptability, which backs up their massive proliferations that often result in harmful blooms (red tides). In this study, we used a bioinformatics approach to identify homologs of known ion channels that belong to 36 ion channel families. We demonstrated that the versatility of the dinoflagellate physiology is underpinned by a high diversity of ion channels including homologs of animal and plant proteins, as well as channels unique to protists. The analysis of 27 transcriptomes allowed reconstructing a consensus ion channel repertoire (channelome) of dinoflagellates including the members of 31 ion channel families: inwardly-rectifying potassium channels, two-pore domain potassium channels, voltage-gated potassium channels (K_v), tandem K_v, cyclic nucleotide-binding domain-containing channels (CNBD), tandem CNBD, eukaryotic ionotropic glutamate receptors, large-conductance calcium-activated potassium channels, intermediate/small-conductance calcium-activated potassium channels, eukaryotic single-domain voltage-gated cation channels, transient receptor potential channels, two-pore domain calcium channels, four-domain voltage-gated cation channels, cation and anion Cys-loop receptors, small-conductivity mechanosensitive channels, large-conductivity mechanosensitive channels, voltage-gated proton channels, inositole-1,4,5-trisphosphate receptors, slow anion channels, aluminum-activated malate transporters and quick anion channels, mitochondrial calcium uniporters, voltage-dependent anion channels, vesicular chloride channels, ionotropic purinergic receptors, animal volage-insensitive cation channels, channelrhodopsins, bestrophins, voltage-gated chloride channels H⁺/Cl^- exchangers, plant calcium-permeable mechanosensitive channels, and trimeric intracellular cation channels. Overall, dinoflagellates represent cells able to respond to physical and chemical stimuli utilizing a wide range of G-protein coupled receptors- and Ca²⁺-dependent signaling pathways. The applied approach not only shed light on the ion channel set in dinoflagellates, but also provided the information on possible molecular mechanisms underlying vital cellular processes dependent on the ion transport.

• Korean Journal of Acupuncture
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• v.28 no.3
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• pp.85-98
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• 2011

Objectives : The aim of this thesis is to examine the effect of acupuncture treatment at the GB37 on normal humans by using the power spectral analysis of the EEG. Methods : EEG (Electroencephalogram) power spectrum exhibits site-specific and state-related differences in specific frequency bands. In this thesis, the power spectrum was measured by the complexity. the 32 channels EEG study was carried out in the 13 subjects (12 males ; age=22.58 years old, 1 females ; 22 years old). Results : In the ${\alpha}$ (alpha) band, the power values at F7, F3, F4, F8, FTC2, C4, T4, CP1, CP2, TCP2, TT2, Pz, P4, Po1, Po2, O1, Oz, O2 channels (p<0.05) during the GB37-acupoint treatment were significantly changed. And in many channels were decreased. In the ${\beta}$ (beta) band, the power values at Cz, C4, T4, Tcp1, T6, Po1, O1, Oz, O2 channels (p<0.05) during the GB37-acupoint treatment were significantly changed. And in many channels were decreased. In the ${\delta}$(delta) band, the power values at Fp1, TT2 channels (p<0.05) during the GB37-acupoint treatment were significantly changed. And in many channels were decreased. In the $\theta$ (theta) band, the power values at Fp1, F8, FTC2, Pz channels (p<0.05) during the GB37-acupoint treatment were significantly changed. And in many channels were decreased. Conclusions : This results suggest that the acupuncture treatment at the GB37 significantly mostly change the power spectrum value on the alpha (18 channels), beta (9 channels) bands.

• BMB Reports
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• v.48 no.1
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• pp.1-5
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• 2015

Transient Receptor Potential, Melastatin-related, member 4 (TRPM4) channels are $Ca^{2+}$-activated $Ca^{2+}$-impermeable cation channels. These channels are expressed in various types of mammalian tissues including the brain and are implicated in many diverse physiological and pathophysiological conditions. In the past several years, the trafficking processes and regulatory mechanism of these channels and their interacting proteins have been uncovered. Here in this minireview, we summarize the current understanding of the trafficking mechanism of TRPM4 channels on the plasma membrane as well as heteromeric complex formation via protein interactions. We also describe physiological implications of protein-TRPM4 interactions and suggest TRPM4 channels as therapeutic targets in many related diseases.

• The Korean Journal of Physiology and Pharmacology
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• v.4 no.6
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• pp.427-437
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• 2000

The early studies of cardiac and smooth muscle cells provided evidence for two different calcium channels, the L-type (also called high-voltage activated [HVA]) and T-type (low-voltage activated [LVA]). These calcium channels provided calcium for muscle contractions and pace-making activities. As might be expected, the number of different calcium channels increased when researchers studied neurons and the identification of the neuronal calcium channels has proven to be much more difficult than with the muscle calcium channels. There are two reasons for this difficulty; (1) a larger number of different calcium channels in neurons and (2) many of the different calcium channels have similar kinetic properties. This review uses the N-type calcium channel to illustrate the difficulties in identifying and characterizing calcium channels in neurons. It shows that the discovery of toxins that can specifically block single calcium channel types has made it possible to easily and rapidly discern the physiological roles of the different calcium channels in the neuron, Without these toxins it is unlikely that progress would have been as rapid.

• The Transactions of the Korean Institute of Electrical Engineers P
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• v.63 no.2
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• pp.81-88
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• 2014

In this paper, an implementation of concurrent backoff delay process on a single chip with IEEE 802.15.4 hardware and 8051 processor core that can be used for analyzing the interference on IEEE 802.15.4 channels due to WiFi traffics is studied. The backoff delay process of IEEE 802.15.4 CSMA-CA algorithm is explained. The characteristics of random number generator, timer, and CCA register included in the single chip are described with their control procedure in order to implement the process. A concurrent backoff delay process to evaluate multiple IEEE 802.15.4 channels is proposed, and a method to service the associated tasks at sequentially ordered backoff delay events occurring on the channels is explained. For the implementation of the concurrent backoff delay process on a single chip IEEE 802.15.4 hardware, the elements for the single channel backoff delay process and their control procedure are used to be extended to multiple channels with little modification. The medium access delay on each channel, which is available after execution of the concurrent backoff delay process, is displayed on the LCD of an IEEE 802.15.4 channel analyzer. The experimental results show that we can easily identify the interference on IEEE 802.15.4 channels caused by WiFi traffics in comparison with the way displaying measured channel powers.

• Journal of Korea Society of Digital Industry and Information Management
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• v.4 no.1
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• pp.17-24
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• 2008

This paper describes the measurements that provide a characterization of the code-domain channels of a CDMA base station transmitter. One of the measurements, called code-domain power(CDP), provides the distribution of power in the code domain channels. This measurement can be used to verify that the various channels are at expected power levels and to determine when one code channel is leaking energy into the other code channels. We develop module of CDP measurement in the CDMA system.

• International Journal of Oral Biology
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• v.31 no.4
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• pp.135-140
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• 2006

Temperature signaling can be initiated by members of transient receptor potential (thermo-TRP) channels. Hot and cold substances applied to teeth usually elicit pain sensation. Since odontoblasts constitute a well-defined layer between the pulp and the mineralized dentin, being first to encounter thermal stimulation from oral cavity, they may be involved in sensory transduction process, in addition to their primary function as formation of dentin. We investigated whether thermo-TRP channels are expressed in a odontoblast cell line, MDPC-23. The expressions of thermo-TRP channels were examined using reverse transcription polymerase chain reaction (RT-PCR), immunohistochemistry, fluorometric calcium imaging. Analysis of RT-PCR revealed mRNA expression of TRPV1, TRPV2, TRPV4 and TRPM8, but no TRPV3, TRPA1. Immunohistochemical approach failed to detect TRPV1 expression. Whereas the application of 4-phorbol-12,13-didecanoate($10\;{\mu}M$, a TRPV4 agonist), menthol(1 mM, a TRPM8 agonist) and icilin($10\;{\mu}M$, a TRPM8 agonist) produced the enhancement of intracellular calcium concentration, capsaicin($1\;{\mu}M$, a TRPV1 agonist) did not. Our results suggest that subfamily of thermo-TRP channels expressed in odontoblasts may serve as thermal or mechanical transducer in teeth.

• Journal of Internet Computing and Services
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• v.15 no.2
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• pp.19-31
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• 2014

In this paper, a parallel backoff delay procedure on multiple IEEE 802.15.4 channels and a channel searching method considering the frequency spectrum of WiFi traffic are studied for IEEE 802.15.4 nodes to avoid the interference from WiFi traffic. In order to search the channels being occupied by WiFi traffic, we analyzed the methods measuring the powers of adjacent channels simultaneously, checking the duration of measured power levels greater than a threshold, and finding the same periodicity of sampled RSSI data as the beacon frame by signal processing. In an wireless channel overlapped with IEEE 802.11 network, the operation of CSMA-CA algorithm for IEEE 802.15.4 nodes is explained. A method to execute a parallel backoff procedure on multiples IEEE 802.15.4 channels by an IEEE 802.15.4 device is proposed with the description of its algorithm. When we analyze the data measured by the experimental system implemented with the proposed method, it is observed that medium access delay times increase at the same time in the associated IEEE 802.15.4 channels that are adjacent each other during the generation of WiFi traffic. A channel evaluation function to decide the interference from other traffic on an IEEE 802.15.4 channel is defined. A channel searching method considering the channel evaluations on the adjacent channels together is proposed in order to search the IEEE 802.15.4 channels interfered by WiFi, and the experimental results show that it correctly finds the channels interfered by WiFi traffic.

• The Korean Journal of Physiology and Pharmacology
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• v.12 no.2
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• pp.51-58
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• 2008

Cardiac fibroblasts constitute one of the largest cell populations in the heart, and contribute to structural, biochemical, mechanical and electrical properties of the myocardium. Nonetheless, their cardiac functions, especially electrophysiological properties, have often been disregarded in studies. $Ca^{2+}$-activated $K^+\;(K_{Ca})$ channels can control $Ca^{2+}$ influx as well as a number of $Ca^{2+}$-dependent physiological processes. We, therefore, attempted to identify and characterize $K_{Ca}$ channels in rat Cardiac fibroblasts. First, we showed that the cells cultured from the rat ventricle were cardiac fibroblasts by immunostaining for discoidin domain receptor 2 (DDR-2), a specific fibroblast marker. Secondly, we detected the expression of various $K_{Ca}$ channels by reverse transcription polymerase chain reaction (RT-PCR), and found all three family members of $K_{Ca}$ channels, including large conductance $K_{Ca}$ (BK-${\alpha}1-\;and\;-{\beta}1{\sim}4$subunits), intermediate conductance $K_{Ca}$ (IK), and small conductance $K_{Ca}$ (SK$1{\sim}4$ subunits) channels. Thirdly, we recorded BK, IK, and SK channels by whole cell mode patch clamp technique using their specific blockers. Finally, we performed cell proliferation assay to evaluate the effects of the channels on cell proliferation, and found that the inhibition of IK channel increased the cell proliferation. These results showed the existence of BK, IK, and SK channels in rat ventricular fibroblasts and involvement of IK channel in cell proliferation.