• Proceedings of the Korea Water Resources Association Conference
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• 2007.05a
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• pp.1824-1828
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• 2007

본 연구에서는 농촌, 산림 및 도시유역에서 발생하는 비점원오염이 하천 수질에 미치는 영향에 관하여 분석하였다. 연구 유역으로는 농촌유역인 유포리, 산림유역인 학술림 및 도시유역인 공지천 등 3개 유역을 선정하였다. 유포리 유역의 면적은 $1.96\;km^2$으로 토지이용은 밭 7.5%, 논 11%, 과수원 1.95%를 제외한 70%가 임야지역으로 나타났다. 산림유역은 면적이 $3.9\;km^2$으로 임산실습을 위한 제탄소 등 교육 및 연구시설을 제외한 대부분(99%)이 산림으로 잘 보전되어 있다. 춘천시에 위치한 공지천 유역은 구시가지와 산림으로 구성되어 있고, 면적은 $0.19\;km^2$중 불투수면적이 $0.15\;km^2$으로 유역면적의 81.9%를 차지한다. 연구유역별로 강우시에는 5분, 건기시에는 30분 간격으로 수위를 측정한 후 수위-유량 곡선을 이용하여 유량으로 환산하였다. 수질 자료는 강우시에는 1일 3회 이상, 건기시에는 2주일에 1회 채취 하여 T-N과 T-P의 분석항목을 환경부 제정 수질 공정시험법의 제반 규정에 따라 분석하여 유역별 오염부하를 산정하였다. 농촌 및 산림유역의 T-N과 T-P의 평균농도는 8.3 mg/L 및 0.3mg/L, 2.4 mg/L 및 0.1 mg/L로 나타났고 도시유역의 건기시와 강우시의 T-N과 T-P의 평균농도는 28.1 mg/L 및 3.1 mg/L, 20.6 mg/L 및 2.5 mg/L로 나타났다. 농촌, 산림 및 도시유역의 T-N과 T-P의 연오염부하는 각각 270.60 kg/ha 및 8.64 kg/ha, 223.16 kg/ha 및 13.87 kg/ha 그리고 612.37 kg/ha 및 69.14 kg/ha이 발생하였다. 100mm 이상의 강우가 발생한 $6{\sim}9$월의 유포리와 학술림의 T-N과 T-P의 오염부하가 연오염부하의 86% 및 88% 그리고 66% 및 82%로 나타나 강우량이 오염부하에 큰 영향을 미치는 것으로 나타났다. 그러나 도시유역의 오염부하는 강우량이 많은 $6{\sim}8$월보다 강우량이 적은 5월과 12월에 크게 나타났다. 토지이용별 T-N과 T-P의 연오염부하는 도시>농촌>산림유역의 순서로 나타났다. 공장, 가정하수, 생활하수 등의 오염원이 많은 도시유역에서 T-N의 오염부하량은 농촌 및 산림유역보다 $2{\sim}3$배 높게 이상 나타났으며 T-P의 경우는 $5{\sim}9$배 이상 높게 나타나 비점원오염 배출은 하천 주변의 토지이용이 큰 영향을 미치는 것으로 판단된다.

• The Korean Journal of Malacology
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• v.13 no.2
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• pp.185-192
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• 1997

1990젼 3월부터 1991년 2월까지 전라북도 군산시 내초도 연안에 서식하는 동죽의 성장을 조사하였다. 1.조사해역의 연간 수온분포는 2.0-26.3$^{\circ}C$이고, 간석지 온도는 0.7-36.2$^{\circ}C$이었다. 2. 동죽의 패각에 나타나는 윤문은 연 1회 형성되며 윤문 형성시기는 3-4월로 조사되었다. 3. 초륜 형성기간은 9개월(0.75년)로 나타났다. 4. 각장(SL: mm)과 체중(TW: G)간의 관계는 TW=1.090 x $10^{-4}$ S $L^{3.2798}$( $r^{2}$=0.99)이었으며, 각장과 각고(SH:mm)간의 관계는 SH=0.865 SL + 0.250( $r^{2}$=0.99)이고, 각장과 각폭(SW: mm) 간의 관계는 SW=0.599 SL - 0.203 ( $r^{2}$=0.98; 각장 22 mm미만), SW=0.724 SL - 2.796( $r^{2}$=0.91; 각장 22 mm이상)이었다.5. 연령(t)에 대한 각장(S $L_{t}$ )의 Bertalanffy성장식은 $L_{t}$ =51.399(1- $e^{0.585}$8(t+0.064)이고, 연령(t)에 대한 체중 (T $W_{t}$ )의 Bertalanffy성장식은 $W_{t}$ =44.467(1- $e^{0.585}$8(t+0.064)$^{3}$로 나타났다.타났다.

• Journal of Life Science
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• v.30 no.6
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• pp.532-541
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• 2020

In this study, we describe the inhibition of adipocyte differentiation by the lactic acid bacteria (LAB) fermentation product of Chrysanthemum indicum L. (CI) extract to control obesity. Preparation of LAB-fermented products was performed to overcome the cytotoxicity of CI extract. During fermentation and 3T3-L1 cell line experiment, cytotoxicity was not induced in the CI fermentation products over 1 day in culture. Fermented materials from highly proliferative cultures were selected for treatment of 3T3-L1 cells and for comparison with unfermented control groups. Cell survival and undifferentiated cell populations were decreased differentiation population in all experimental groups compared with controls, as measured using fluorescence-activated cell sorting analysis. Akt pathway activity increased upon treatment with these fermented extracts in 3T3-L1 cells. Gli2 depleted at the protein level in association with adipocyte differentiation. LAB KCTC 3115- and 3109-fermented extract treatment caused controlled Gli2 protein accumulation. Moreover, KCTC 3115 and 3109 were found to reduce C/EBPα and FAS was depleted, whereas pACC was increased at the protein level upon treatment with the fermentation products of each of the four LAB used in this study. With Lactococcus lactis subsp. lactis KCTC 3115 fermentation, the regulation of adipose differentiation and hedgehog signaling were also suppressed, thereby inhibiting the differentiation of progenitor cells. The basis for the activation of hedgehog signaling may provide insights into the treatment of obesity and the inhibition of adipocyte differentiation.

• Natural Product Sciences
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• v.17 no.4
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• pp.273-278
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• 2011

In this study, the effects of a methanol (MeOH) extract of Carduus crispus L. (Asteraceae) on adipogenesis was investigated in 3T3-L1 cells. To differentiate preadipocytes to adipocytes, confluent 3T3-L1 preadipocytes were treated with a hormone mixture, which included isobutylmethylxanthine, dexamethasone, and insulin (MDI). The methanol extract of C. crispus significantly decreased fat accumulation by inhibiting adipogenic signal transcriptional factors in MDI-induced 3T3-L1 cells in a dose-dependent manner. In MTT assays and on PI-staining, methanol extract of C. crispus inhibited the proliferation of 3T3-L1 cells during mitotic clonal expansion (MCE). The anti-adipogenic effect of the Carduus extract seemed to be associated with the upregulation of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (MAPK) pathways within the first 2 days after MDI treatment. These results suggest that methanol extract of C. crispus might be beneficial for the treatment of obesity.

• Korean Journal of Environmental Agriculture
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• v.18 no.2
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• pp.126-134
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• 1999

To provide the basic information for the water improvement and control of water resource in Nakdong river districts, the physico-chemical characteristics of water in four main streams and three branch streams of the river were investigated through 36 times totally, one time per each month from January in 1995 to November in 1997. The pH values of each sites in main or branch stream of Nakdong river was pH $6.3{\sim}9.3$ range, pH range of Jukpo and Namji area at spring and fall was almost over pH 8.5, which was exceeded the water quality standard for agriculture. DO values of Nakdong river was almost $8.0{\sim}13mg/l$, except for Kangchang area in downstream of Kumho river. BOD values in Dasa area where was prior to mixing of Kumho river was $1.5{\sim}4.8mg/l$, which was under the water quality standard for agriculture(8mg/l), but BOD values followed mixing of Kumho river was over the permit standard of agricultural water as $3.8{\sim}8.9mg/l$ in Koryung, $3.4{\sim}8.4mg/l$ in Jukpo and $3.3{\sim}7.8mg/l$ in Namji according to time or season. Especially, BOD values at Gangchang area in Kumho river were $7.6{\sim}18.5mg/l$, which was over the water quality standard for agriculture and so Kumho river was a main pollutant: source of Nakdong river. COD values of main stream of Nakdong river was over the permit standard of agriculture(8mg/l) as $5.2{\sim}13.5mg/l$ in Koryvng, $5.0{\sim}12.7mg/l$ in Jukpo and $5.0{\sim}12.2mg/l$ in Namji according to time or season. And COD values was much high rather than BOD values and its gap of concentration was increased along with downstream. $NH_4-N$ of main stream of Nakdong river followed mixing of Kumho river($0.5{\sim}13.1mg/l$) was the highest affected in Koryung($0.18{\sim}5.0mg/l$) and detected much more in winter than in summer. T-N in Koryung($4.96{\sim}12.06mg/l$) followed mixing of Kumho river was significantly high rather than $2.86{\sim}4.86mg/l$ in Dasa, $4.20{\sim}8.20mg/l$ in Jukpo and $3.18{\sim}8.64mg/l$ in Namji, which was almost over the permit standard of agricultural water(1.0mg/l). T-P in Koryung($0.10{\sim}0.58mg/l$) also was significantly high rather than those $0.07{\sim}0.36mg/l$ in Jukpo and $0.08{\sim}0.4mg/l$ in Namji as over the standard of agricultural water(0.1mg/l). The concentration of T-N or T-P in Nakdong districts was trended of increasing in every year.

• Clean Technology
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• v.7 no.2
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• pp.89-97
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• 2001

The purpose of this study was to find the optimum condition of the high removal efficiency of water pollutant as sewage treatment technology using a filtering bed charged with porous sintering-pellet which was planted with hydrophytes. Experiment was carried out by changing concentration of water pollutants(COD, T-N, T-P), kind of hydrophyte, kinds of filtering material and size, and HRT. The result of removal efficiency was obtained as following: COD removal 73.8~87.1% for input concentration range of 50~450mg/L, T-N removal 61.3~77.3% for input concentration range of 7~124mg/L, T-P removal 89.5~99.1% for input concentration ranger of 3~27mg/L. In a comparative experiment of three kinds of hydrophyte(Iris pseudoacorus, Phragmites communis $T_{RIN}$., Oenanthe javanica Dc.), the best removal efficiency of COD and T-N was gained with Iris pse-udoacorus, and Phragmites communis $T_{RIN}$ showed better result than two hydrophytes for the removal efficiency of T-P. In a comparative experiment of four kinds of filtering-materials, the removal efficiencies were in the order of porous sintering-pellet, gravel, nonused-tire and nonused-concrete. It was found that for the porous sintering-pellet, the smaller its diameter, the better its result. In the filtering bed in which was charged with porous sintering-pellets of 5mm diameter and planted with Iris pseudoacorus, the removal efficiency of COD, T-N and T-P were over 80%, 70% and 90% under the concentration of COD 250mg/L, T-N 70mg/L and T-P 15mg/L for 24hrs treatment. Thus, we concluded that a filtering bed charged with porous sintering-pellet and planted with hydrophytes will be suitable for treatment of sewage water as a pro-natural treatment technology.

• Food Science and Preservation
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• v.19 no.3
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• pp.455-461
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• 2012

Obesity, a strong risk factor for the development of chronic diseases, is characterized by an increase in the number and size of adipocytes differentiated from precursor cells, preadipocytes. Recent research suggests that increased reactive oxygen species (ROS) production in 3T3-L1 adipocyte facilitates adipocyte differentiation and fat accumulation. This study was to investigate whether reduced ROS production by Sargassum micracanthum extract (SME) could protect the development of obesity through inhibition of adipogenesis. 3T3-L1 preadipocytes were treated SME for up to 8 days following standard induction of differentiation. The extent of differentiation reflected by amount of lipid accumulation and ROS production was determined by Oil red O staining and nitroblue tetrazolium (NBT) assay. Treatment of SME significantly inhibited ROS production and adipocyte differentiation that is depend on down regulation of NADPH oxidase 4 (NOX4), a major ROS generator, and peroxisome proliferator-activated receptor gamma ($PPAR{\gamma}$) and CCAAT/enhancer-binding protein alpha ($C/EBP{\alpha}$), a key adipogenic transcription factor. These results indicate that SME can inhibit adipogenesis through a reduced ROS level that involves down-regulation of NOX4 expression or via modulation of adipogenic transcription factor.

• Food Science of Animal Resources
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• v.32 no.4
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• pp.512-519
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• 2012

The current study was conducted to investigate the effect of macsumsuk and herb resources on the performances and meat quality of broiler chickens. Six hundreds (600) broiler chickens were randomly allocated into four groups (4 groups${\times}$50 chickens/group${\times}$3 replica), Control, Treatment 1 (T1; 0.3% macsumsuk), Treatment 2 (T2; 0.3% herb resources), and Treatment 3 (T3; 0.3% macsumsuk + 0.3% herb resources) and fed for 5 wk. T2 group showed higher total body weight gain and average daily gain of 1,812.5 g and 51.79 g, respectively, than the other groups. Control group showed the highest (p<0.05) mortality (8%) and total blood cholesterol (111.8 mg/dL) among experimental groups. T3 group (6.71 mg/dL) showed the highest (p<0.05) while control group (4.50 mg/dL) showed the lowest (p<0.05) in blood IgG levels. Cooking loss was 17.08, 16.14, 16.55, and 15.25%, shear force value was 1.91, 1.52, 1.55, and 1.47 $kg/cm^2$, and water holding capacity (WHC) was 54.40, 55.97, 56.01, and 55.70% for Control, T1, T2, and T3, respectively. Cholesterol contents in breast meat of Control (88.91 mg/100 g) chickens showed the highest (p<0.05) levels comparing to either T1 (83.59 mg/dL), T2 (82.41 mg/dL), or T3 (80.81 mg/dL) chickens. In conclusion, the current study implies that feeding macsumsuk and herb resources to broiler chickens could decrease cholesterol contents in breast meat.

• Journal of Physiology & Pathology in Korean Medicine
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• v.24 no.1
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• pp.118-123
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• 2010

Piper nigrum Linne has been used spice as well as herbal medicine in worldwide and has function of anti-oxidant, anti-inflammation, anti-cancer, bioavailability of drugs and etc. In this study, anti-adipogenic activity of Piper nigrum Linne extract and its constituent piperine were investigated in 3T3-L1 differentiation. Adipogenic effects and lipid accumulation in 3T3-L1 was measured by RT-PCR and Oil Red O staining assays respectively in stimulation of Piper nigrum Linne extract and piperine. Piper nigrum Linne and piperine reduced lipid accumulation in 3T3-L1 differentiation and expression of genes associated with adipogenesis such as PPAR${\gamma}$, adipsin, SERBP-1c and LPL. All of taken, anti-adipogenic mechanism of Piper nigrum Linne and piperine were related with regulation of SREBP-1c and PPAR${\gamma}$ expression.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.9
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• pp.1342-1348
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• 2014

Seeds of Korean pine cone (Pinus koraiensis) have long been consumed as an edible food in countries located in North-East Asia, On the other hand, Korean pine cone, containing various polyphenols, is discarded as a useless garbage after removing seeds. This study investigated the lipolytic effects of pine cone extract in differentiated 3T3-L1 adipocytes. Intracellular lipid accumulation was measured by Oil red O staining, free glycerol release by colorimetric reaction, and expression of genes related to lipid metabolism by real-time PCR. Compared to control, pine cone extract reduced intracellular lipid accumulation by 8.8% and increased free glycerol release by 8.2% a concentration of $5{\mu}g/mL$ in differentiated 3T3-L1 adipocytes. mRNA levels of fatty acid synthesis were not significantly different between control and pine cone extract, but mRNA levels of lipoprotein lipase (LPL) and hormone-sensitive lipase (HSL) significantly increased by 38.7% and 94.1% at a concentration of $5{\mu}g/mL$, respectively. Thus, pine cone extract is suggested to have lipolytic effects through induction of LPL and HSL gene expression.