Browse > Article
http://dx.doi.org/10.3746/jkfn.2014.43.9.1342

Lipolytic Effect of Supercritical Extraction from Pine Cone (Pinus koraiensis) in Mature 3T3-L1 Adipocytes  

Lee, Minhee (Department of Medical Nutrition and 3Research Institute of Medical Nutrition, Kyung Hee University)
Nam, Da-Eun (Department of Medical Nutrition and 3Research Institute of Medical Nutrition, Kyung Hee University)
Kim, Ok Kyung (Department of Medical Nutrition and 3Research Institute of Medical Nutrition, Kyung Hee University)
Heo, Seok Hyun (Korea Health Supplements Association)
Lee, Jeongmin (Department of Medical Nutrition and 3Research Institute of Medical Nutrition, Kyung Hee University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.43, no.9, 2014 , pp. 1342-1348 More about this Journal
Abstract
Seeds of Korean pine cone (Pinus koraiensis) have long been consumed as an edible food in countries located in North-East Asia, On the other hand, Korean pine cone, containing various polyphenols, is discarded as a useless garbage after removing seeds. This study investigated the lipolytic effects of pine cone extract in differentiated 3T3-L1 adipocytes. Intracellular lipid accumulation was measured by Oil red O staining, free glycerol release by colorimetric reaction, and expression of genes related to lipid metabolism by real-time PCR. Compared to control, pine cone extract reduced intracellular lipid accumulation by 8.8% and increased free glycerol release by 8.2% a concentration of $5{\mu}g/mL$ in differentiated 3T3-L1 adipocytes. mRNA levels of fatty acid synthesis were not significantly different between control and pine cone extract, but mRNA levels of lipoprotein lipase (LPL) and hormone-sensitive lipase (HSL) significantly increased by 38.7% and 94.1% at a concentration of $5{\mu}g/mL$, respectively. Thus, pine cone extract is suggested to have lipolytic effects through induction of LPL and HSL gene expression.
Keywords
pine cone; Pinus koraiensis; 3T3-L1; lipolysis;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 Bray GA, Tartaglia LA. 2000. Medicinal strategies in the treatment of obesity. Nature 404: 672-677.
2 Jin JA, Oh KS, Hyun YN, Choi SK, Kwon YH, Kim HJ, Woo B. 2013. Antiadipogenic effect of Vitis amurensis root methanol extract and its solvent fraction in 3T3-L1 preadipocytes. J Life Sci 23: 69-78.   DOI   ScienceOn
3 Spiegelman BM, Flier JS. 1996. Adipogenesis and obesity; rounding out the big picture. Cell 87: 377-389.   DOI   ScienceOn
4 Visscher TL, Seidell JC. 2001. The public health impact of obesity. Annu Rev Public Health 22: 355-375.   DOI   ScienceOn
5 Spiegelman BM, Flier JS. 2001. Obesity and the regulation of energy balance. Cell 104: 531-543.   DOI   ScienceOn
6 Ardévol A, Bladé C, Salvadó MJ, Arola L. 2000. Changes in lipolysis and hormone-sensitive lipase expression caused by procyanidins in 3T3-L1 adipocytes. Int J Obes Relat Metab Disord 24: 319-324.   DOI
7 Balkau B, Valensi P, Eschwege E, Slama G. 2007. A review of the metabolic syndrome. Diabetes Metab 33: 405-413.   DOI
8 Holst D, Grimaldi PA. 2002. New factors in the regulation of adipose differentiation and metabolism. Curr Opin Lipidol 13: 241-245.   DOI   ScienceOn
9 De Ferranti S, Mozaffarian D. 2008. The perfect storm: obesity, adipocyte dyfunction, and metabolic consequences. Clin Chem 54: 945-955.   DOI   ScienceOn
10 Padwal RS, Majumar SR. 2007. Drug treatments for obesity: orlistat, sibutramine, and rimonabant. Lancet 369: 71-77.   DOI   ScienceOn
11 Zhi J, Moore R, Kanitra L, Mulligan TE. 2003. Effects of orlistat, a lipase inhibitor, on the pharmacokinetics of three highly lipophilic drugs (amiodarone, fluoxetine, and simvastatin) in healthy volunteers. J Clin Pharmacol 43: 428-435.   DOI   ScienceOn
12 Lee HJ, Choe YJ, Choe DH, Hong IP. 2003. Extractives of Pinus koraiensis wood. J Korean Wood Sci Technol 31:49-56.
13 Kim KK, Cho HJ, Kang HC, Youn BB, Lee KR. 2006. Effects on weight reduction and safety of short-term phentermine administration in Korean obese people. Yonsei Med J 47: 614-625.   DOI   ScienceOn
14 Cercato C, Roizenblatt VA, Leanca CC, Segal A, Lopes Filho AP, Mancini MC, Halpern A. 2009. A randomized doubleblind placebo-controlled study of the long-term efficacy and safety of diethylpropion in the treatment of obese subjects. Int J Obes 33: 857-865.   DOI
15 Critchfield WB, Little EL Jr. 1966. Geographic Distribution of the Pines of the World. US Department Agriculture, Forest Service, Washington, DC, USA. No 991, p 4.
16 Cimanga K, Kambu K, Tona L, Apers S, De Bruyne T, Hermans N, Totte J, Pieters L, Vlietinck AJ. 2002. Correlation between chemical composition and antibacterial activity of essential oils of some aromatic medicinal plants growing in the Democratic Republic of Congo. J Ethopharmacol 79:213-220.   DOI   ScienceOn
17 Baricevic D, Milevoj L, Borstnik J. 2001. Insecticidal effect of oregano Origanum vulgare L. ssp. hirtum Ietswaart) on bean weevil (Acanthoscelides obtectus Say). Inter J Horticult Sci 7: 84-88.
18 Hong WT, Ko KM, Lee JG, Jang HJ, Kwak JJ. 2002. Volatile compounds of pine needle (Pinus rigida miller) extracts. J Korea Soc Tabacco Sci 24: 53-59.
19 Kim YK, Chung KN, Hirosh I, Shigeru M. 1986. Volatile components of pinenut. Korean J Food Sci Technol 18: 105-109.
20 Caldefie-Chézet F, Guerry M, Chalchat JC, Fusillier C, Vasson MP, Guillot J. 2004. Anti-inflammatory effects of Melaleuca alternifolia essential oil on human polymorphonuclear neutrophils and monocyte. Free Radic Res 38:805-811.   DOI   ScienceOn
21 Hwang HJ, Yu JS, Lee HY, Kwon DJ, Han W, Heo SI, Kim SY. 2014. Evaluations on deodorization effect and anti-oral microbial activity of essential oil from Pinus koraiensis. Korean J Plant Res 27: 1-10.   DOI
22 Medeiros R, Passos GF, Vito CE, Koepp J, Mazzuco TL, Pianowski LF. 2007. Effect of two active compounds obtained from the essential oil of Cordia verbenacea on the acute inflammatory responses elicited by LPS in the rat paw. Br J Pharmacol 151: 618-627.   DOI   ScienceOn
23 Sylvestre M, Pichette A, Longtin A, Nagau F, Legault J. 2006. Essential oil analysis and anticancer activity of leaf essential oil of Croton flavens L. from Guadeloupe. J Ethnopharmacol 103: 99-102.   DOI   ScienceOn
24 Lee EK, Kim HS, Chung SJ. 2011. Antimicrobial activity of chitosan solution with PKS (Pinus koraiensis S. et Z) and PRM (Pinus rigida Mill) extract. J Chitin Chitosan 16:117-122.
25 Kim CH, Lee SY, Pak JI. 2008. The extraction of essential oil from by-product of pine nut cone and its antibacterial activity. Annal Animal Resource Sci 19: 63-70.
26 Ministry of Agriculture, Food and Rural Affairs. 2010. Statistical yearbook of forestry. Vol 36, p 296.
27 Kim JE, Kim WY, Kim JW, Park HS, Lee SH, Lee SY, Kim MJ, Kim AR, Park SN. 2010. Antibacterial, antioxidative activity and component analysis of Pinus koraiensis leaf extracts. J Soc Cosmet Scientists Korea 36: 303-314.
28 Berridge MV, Tan AS. 1993. Characterization of the cellular reduction of 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT): subcellular localization, substrate dependence, and involvement of mitochondrial electron transport in MTT reduction. Arch Biochem Biophys 303:474-482.   DOI   ScienceOn
29 Green H, Kehinde O. 1975. An established preadipose cell line and its differentiation in culture. II. Factors affecting the adipose conversion. Cell 5: 19-27.   DOI   ScienceOn
30 Ailhaud G, Grimaldi P, Negrel R. 1992. Cellular and molecular aspects of adipose tissue development. Annu Rev Nutr 12: 207-233.   DOI   ScienceOn
31 Trinder P. 1969. Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen. J Clin Pathol 22: 158-161.   DOI
32 Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB. 1987. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of radiosensitivity. Cancer Res 47: 943-946.
33 Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB. 1987. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res 47: 936-942.
34 Haugen F, Zahid N, Dalen KT, Hollung K, Nebb HI, Drevon CA. 2005. Resistin expression in 3T3-L1 adipocytes is reduced by arachidonic acid. Lipid Res 46: 143-153.
35 Tenney R, Stansfield K, Oekala PH. 2005. Interleukin 11 signaling in 3T3-L1 adipocytes. J Cell Physiol 202: 160-166.   DOI   ScienceOn
36 MacDougald OA, Hwang CS, Fan H, Lane MD. 1995. Regulated expression of the obese gene product (leptin) in white adipose tissue and 3T3-L1 adipocytes. Proc Natl Acad Sci USA 92: 9034-9037.   DOI   ScienceOn
37 Frayn KN, Coppack SW, Fielding BA, Humphreys SM. 1995. Coordinated regulation of hormone sensitive lipase and lipoprotein lipase in human adipose tissue in vivo: implications for the control of fat storage and fat mobilization. Adv Enzyme Regul 35: 163-178.   DOI
38 Kim HJ, Yun YR, Song YB, Song YO. 2008. Ani-lipogenic effects of tannic acid in 3T3-L1 adipocytes and in high fat diet-fed rats. Food Sci Biotechnol 17: 219-445.
39 Ronnett GV, Kim EK, Landree LE, Tu Y. 2005. Fatty acid metabolism as a target for obesity treatment. Physiol Behav 85: 23-35.
40 Langin D, Holm C, Lafontan M. 1996. Adipocyte hormone sensitive lipase: a major regulator of lipid metabolism. Proc Nutr Soc 55: 93-109.   DOI
41 Jeon T, Hwang SG, Hirai S, Matsui T, Yano H, Kawada T, Lim BO, Park DK. 2004. Red yeast rice extracts suppress adipogenesis by downregulating adipogenic transcription factors and gene expression in 3T3-L1 cells. Life Sci 75:3195-3203.   DOI   ScienceOn
42 Rea S, James DE. 1997. Moving GLUT4: the biogenesis and trafficking of GLUT4 storage vesicles. Diabetes 46: 1667-1677.   DOI
43 Mead JR, Irvine SA, Ramji DP. 2002. Lipoprotein lipase: structure, function, regulation, and role in disease. J Mol Med 80: 753-769.   DOI   ScienceOn
44 Morimoto C, Kameda K, Tsujita T, Okuda H. 2001. Relationships between lipolysis induced by various lipolytic agents and hormone sensitive lipase in rat fat cells. J Lipid Res 42: 120-127.
45 Kim KB, Jang S. 2014. Anti-obesity effect of EGCG and glucosamine-6-phosphate through decreased expression of genes related to adipogenesis and cell cycle arrest in 3T3-L1 adipocytes. J Nutr Health 47: 1-11.   DOI
46 Choi BH, Ahn IS, Kim YH, Park JW, Lee SY, Hyun CK, Do MS. 2006. Berberine reduces the expression of adipogenic enzymes and inflammatory molecules of 3T3-L1 adipocyte. Exp Mol Med 38: 599-605.   DOI   ScienceOn