• 한국지반환경공학회 논문집
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• 제10권7호
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• pp.25-32
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• 2009

본 연구는 팽창질석을 사용하여 수용액 상의 구리이온 흡착 제거능을 평가 하고자 하였다. 질석의 화학적 조성분석은 XRF, 구리이온 농도분석은 UV-VIS를 각각 사용하여 이루어졌다. 수용액 상의 구리이온의 제거양상을 살펴보기 위해 batch kinetic test와 batch sorption test가 실시되었고, 그 결과 구리의 제거속도($K_{obs}$, 1/hr)는 초기pH 3일때 0.73, pH 4일때 1.52, pH 5일때 1.71였고 초기농도가 $1mg\;L^{-1}$ 일때 3.19, 5일때 1.90, 10일때 0.73으로 초기구리농도와 반비례하고 초기 pH와는 비례하여 증가하는 양상을 보였다. 하지만 초기용액의 농도차이보다 초기pH의 영향이 지배적일 것이라는 예상하에 동일한 농도에 pH만 달리하여 실험해본 결과 역시 예상대로 pH에 따라 제거속도가 크게 차이남을 알 수 있었다. 최종적으로 batch sorption test를 통해 얻은 결과를 각각 Freundlich와 Langmuir 등온흡착식에 대입한 결과 두 식 모두 양호한 fitting 결과를 얻을 수 있었으나 Freundlich 식의 결정계수가 0.965로 Langmuir 식의 결정계수 0.936보다 좀 더 높게 나타나 좀 더 정확한 fitting 결과를 보여주었다. Langmuir 모델로부터 얻은 최대흡착용량($Q_{max}$), Freundlich 모델의 분배계수, n 값은 각각 $1,250mg\;kg^{-1}$, $635.1L\;kg^{-1}$, 1.69였다. 이러한 결과는 팽창질석이 다양한 형태의 수용액 상에 존재하는 구리이온을 효과적으로 제거할 수 있음을 보여준다.

• 한국축산식품학회지
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• 제30권4호
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• pp.568-574
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• 2010

This study introduced a chick embryos’ infection model to elucidate the pathogenesis mechanism of Pseudomonas aeruginosa, which causes serious diseases in human after ingestion of P. aeruginosa-contaminated animal originated foods. The embryonic chick model is able to give a rapid and relatively inexpensive method to assess bacterial pathogenicity compared to embryos of other vertebrates. Embryos were infected with P. aeruginosa and elastase-deficient P. aeruginosa. After infection with P. aeruginosa cells, total bacterial cell numbers and gelatinase activities in the embryos were compared. Thereafter, precartilage condensation and chondrogenesis were assessed by peanut agglutinin (PNA) binding on day 3 and by Alcian blue staining for sulfated proteoglycans on day 5, respectively. P. aeruginosa significantly increased in embryos, resulting in abnormal limb development, whereas P. aeruginosa defective in elastase activity partly impaired proliferation. In addition, P. aeruginosa-infected chick embryos significantly stimulated the production of matrix metalloproteinases. Several analyses showed that elevated proteases suppressed the proliferation and survival of chondrogenic cells. The results show that this infection model was a useful assay to determine the virulence mechanism of P. aeruginosa in human after intake of microbiologically contaminated foods.

• 한국식품영양과학회지
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• 제24권3호
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• pp.384-388
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• 1995

어유는 고도불포화지방산 함량이 높아 산화가 쉽게 되므로 식품가공용이나 기능성식품으로 응용되기에는 많은 제약이 따른다. 이러한 문제를 해결하기 위하여 어유의 산화안정성을 예측할 수 있는 기본 모델을 다음과 같은 식을 이용하여 제시하였다. $dp/dt=k{\cdot}p(t){\cdot}[P_{max}\;-\;p(t)]$. 상기식을 적분하면, $p(t)=P_{max}/[1\;+\;[(P_{max}/P_{(0)})\;-\;-1]{\cdot}EXP(-K_p{\cdot}t)]$. 여기서 산화온도가 50, 60와 70 및 $80^{\circ}C$ 일 때, $K_p$는 각각 0.00535, 0.01345와 0.02516 및 0.04657였다. 상기식은 대부분 측정치와 잘 일치하였다. 또한 $K_p$를 아래와 같이 온도의 함수로 표현할 수 있었다. $K_p=(1/P_{max})EXP\;[1\;-\;(8148/T)+20.1]$. 여기서 T 절대온도($^{o}K$)이다.

• 품질경영학회지
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• 제50권3호
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• pp.349-371
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• 2022

Purpose: Compared to the rapid growth rate of the domestic automotive LED industry so far, the predictive analysis method for demand forecasting or market outlook was insufficient. Accordingly, product characteristics are analyzed through the life trend of LEDs for automotive exterior lamps and the relative strengths of p and q using the Bass model. Also, future demands are predicted. Methods: We used sales data of a leading company in domestic market of automotive LEDs. Considering the autocorrelation error term of this data, parameters m, p, and q were estimated through the modified estimation method of OLS and the NLS(Nonlinear Least Squares) method, and the optimal method was selected by comparing prediction error performance such as RMSE. Future annual demands and cumulative demands were predicted through the growth curve obtained from Bass-NLS model. In addition, various nonlinear growth curve models were applied to the data to compare the Bass-NLS model with potential market demand, and an optimal model was derived. Results: From the analysis, the parameter estimation results by Bass-NLS obtained m=1338.13, p=0.0026, q=0.3003. If the current trend continues, domestic automotive LED market is predicted to reach its maximum peak in 2021 and the maximum demand is $102.23M. Potential market demand was $1338.13M. In the nonlinear growth curve model analysis, the Gompertz model was selected as the optimal model, and the potential market size was $2864.018M. Conclusion: It is expected that the Bass-NLS method will be applied to LED sales data for automotive to find out the characteristics of the relative strength of q/p of products and to be used to predict current demand and future cumulative demand.

• Journal of Microbiology and Biotechnology
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• 제32권6호
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• pp.740-748
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• 2022

As circ_UBE2D2 has been confirmed to have targeted binding sites with multiple miRNAs involved in septic acute kidney injury (SAKI), efforts in this study are directed to unveiling the specific role and relevant mechanism of circ_UBE2D2 in SAKI. HK-2 cells were treated with lipopolysaccharide (LPS) to construct SAKI model in vitro. After sh-circ_UBE2D2 was transfected into cells, the transfection efficiency was detected by qRT-PCR, cell viability and apoptosis were determined by MTT assay and flow cytometry, and expressions of Bcl-2, Bax and Cleaved-caspase 3 were quantified by western blot. Target genes associated with circ_UBE2D2 were predicted using bioinformatics analysis. After the establishment of SAKI rat model, HE staining and TUNEL staining were exploited to observe the effect of circ_UBE2D2 on tissue damage and cell apoptosis. The expression of circ_UBE2D2 was overtly elevated in LPS-induced HK-2 cells. Sh-circ_UBE2D2 can offset the inhibition of cell viability and the promotion of cell apoptosis induced by LPS. Circ_UBE2D2 and miR-370-3p as well as miR-370-3p and NR4A3 have targeted binding sites. MiR-370-3p inhibitor reversed the promoting effect of circ_UB2D2 silencing on viability of LPS-treated cells, but shNR4A3 neutralized the above inhibitory effect of miR-370-3p inhibitor. MiR-370-3p inhibitor weakened the down-regulation of NR4A3, Bax and Cleaved caspase-3 and the up-regulation of Bcl-2 induced by circ_UB2D2 silencing, but these trends were reversed by shNR4A3. In addition, sh-circ_UBE2D2 could alleviate the damage of rat kidney tissue. Circ_UBE2D2 mitigates the progression of SAKI in rats by targeting miR-370-3p/NR4A3 axis.

• PNF and Movement
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• 제18권3호
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• pp.305-313
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• 2020

Purpose: The aim of this study was to investigate the relationship between a functional evaluation model and the Fugl-Meyer assessment (FMA) scale in evaluating the upper extremities of stroke patients Methods: Thirty-eight stroke patients were evaluated using the FMA and performed reaching and grasping motions using a three-dimensional motion analysis (Qquas 1 series, Qualisys AB, Sweden). The participants sat on a chair with a backrest. The position of the cup was located at a distance of 80% to the front arm length. The markers were attached to the sternum, acromion, elbow lateral epicondyle, ulnar styloid process, three metacarpal heads, and the distal phalanges of the thumb and index finger. The variables of the correlation between the functional evaluation model and the FMA scale were analyzed. Multiple regression (stepwise) was used to investigate the effect of the kinematic variables. Results: A significant negative correlation was found between the movement time (p < 0.05), movement unit (p < 0.05), and trunk displacement values (p < 0.05) in the FMA total scores, while a positive correlation was found between the peak velocity (p < 0.05) and maximum grip aperture values (p < 0.05). As a result of the multiple regression analysis, the most significant factor was the movement unit, followed by the general movement assessment and trunk displacement. The explained FMA total score value was 62%. Conclusion: This study presents a new functional evaluation model for assessing the reaching and grasping ability of stroke patients. The factors of the proposed functional evaluation model showed significant correlations with the FMA scale scores and confirmed that the new functional evaluation model explained the FMA by 67%. This suggests a new functional evaluation model for reaching and grasping stroke patients.

• 한국전자통신학회논문지
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• 제6권2호
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• pp.157-162
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• 2011

국제전기통신연합(ITU)은 ITU-R을 통해 현재 많은 서비스가 이루어지고 있는 방송 및 통신 분야에서 다양한 전파 환경에 적용할 수 있는 각각의 예측 모델을 권고하고 있다. 각 전파예측모델은 예상되는 많은 채널환경의 차이를 보완하기 위한 절차를 통해 수정되며, 전파예측 판단을 위한 평가 기준으로 준비된다. 본 연구에서는 방송 환경에 적합한 점대 영역(Point-to-Area) 모델, ITU-R Rec. P.1546-3에서, 송신국 스펙이 고려되어 있지 않은 파라미터를 보충함으로써, 각 송신국에서의 요소를 고려한 실질적인 전파 예측 모델의 구현이 가능하도록 하였다.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2011년도 추계학술발표회
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• pp.31-45
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• 2011

The amyloid ${\beta}$-peptide ($A{\beta}$), which originates from the proteolytic cleavage of amyloid precursor protein (APP), plays a central role in the pathogenesis of Alzheimer's disease (AD). Mounting evidence indicates that different species of $A{\beta}$, such as $A{\beta}$ oligomers and fibrils, may contribute to AD pathogenesis via distinct mechanisms at different stages of the disease. Importantly, elevation and accumulation of soluble $A{\beta}$ oligomers closely correlate with cognitive decline and/or disease progression in animal models of AD. In agreement with these studies, oligomers of $A{\beta}$ have been shown to directly affect synaptic plasticity, a neuronal process that is known to be essential for memory formation. Our previous studies showed that $A{\beta}$ induces the breakdown of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), a phospholipid that regulates key aspects of neuronal function. PI(4,5)P2 breakdown was found to be a key step toward synaptic and memory dysfunction in a mouse model of AD. To this end, we seek to identify small molecules that could elevate the levels of PI(4,5)P2 and subsequently block $A{\beta}$ oligomer-induced breakdown of PI(4,5)P2 and synaptic dysfunction.. We found that (20S)Rg3, an active triterpene glycoside from heat-processed ginseng, serves as an agonist for phosphatidylinositol 4-kinase IIalpha (PI4KIIalpha), which is a lipid kinase that mediates a rate-limiting step in PI(4,5)P2 synthesis. Consequently, (20S)Rg3 stimulates PI(4,5)P2 synthesis by directly stimulating the activity of PI4KIIalpha. Interestingly, treatment of a mouse model of AD with (20S)Rg3 leads to reversal of memory deficits. Our data suggest that the PI(4,5)P2-promoting effects of (20S)Rg3 may help mitigate the cognitive symptoms associated with AD.

• 한국수산과학회지
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• 제34권5호
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• pp.521-525
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• 2001

국내에서 다량 생산되는 갈조류인 미역포자엽, 다시마, 톳, 모자반으로부터 fucoidan을 추출, 분획하여 Pb와 Cd의 흡착 특성을 조사하였다. 4종의 갈조류에서 추출한 fucoidan의 분획물 모두 Pb의 흡착량이 Cd보다 높았으며, 시료간에는 미역포자엽 분획물 (Fr-3.0)이 Pb와 Cd 모두 흡착력이 상대적으로 우수하였다. pH와 농도 변화에 따른 홉착력의 변화는 $C_f$ (잔류농도)가 50mg/L까지는 pH 간 차이가 없었으나, 농로가 증가함에 따라 pH 5.5 처리구가 다른 처리구에 비하여 흡착량이 증가하였다. 실험을 통하여 얻은 Pb 와 Cd의 최대 흡착량은 pH 5.5에서 각각 94mg/g ($C_f$164mg/L), 64mg/g ($C_f$197mg/L)였으며 Langmuir sorption model을 통해 구한 최대 흡착량은 pH 5.5에서 각각 178mg/g, 122mg/g이었다. Cd 공존시 Pb 흘착량은 $C_f$가 낮을 범위에서는 변화가 없었으나 $C_f$가 증가함에 따라 공존 이온의 흡착 방해로 인하여 감소하였다.

• Biomolecules & Therapeutics
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• 제24권5호
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• pp.489-494
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• 2016

Neuropathic pain (NPP) is the main culprit among chronic pains affecting the normal life of patients. Procaine is a frequently-used local anesthesia with multiple efficacies in various diseases. However, its role in modulating NPP has not been reported yet. This study aims at uncovering the role of procaine in NPP. Rats were pretreated with procaine by intrathecal injection. Then NPP rat model was induced by sciatic nerve chronic compression injury (CCI) and behavior tests were performed to analyze the pain behaviors upon mechanical, thermal and cold stimulations. Spinal expression of Janus kinase 2 (JAK2) and signal transducer and activator of transcription 3 (STAT3) was detected by qRT-PCR and western blot. JAK2 was also overexpressed in procaine treated model rats for behavior tests. Results showed that procaine pretreatment improved the pain behaviors of model rats upon mechanical, thermal and cold stimulations, with the best effect occurring on the $15^{th}$ day post model construction (p<0.05). Procaine also inhibited JAK2 and STAT3 expression in both mRNA (p<0.05) and protein levels. Overexpression of JAK2 increased STAT3 level and reversed the improvement effects of procaine in pain behaviors (p<0.01). These findings indicate that procaine is capable of attenuating NPP, suggesting procaine is a potential therapeutic strategy for treating NPP. Its role may be associated with the inhibition on JAK2/STAT3 signaling.