Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Procaine Attenuates Pain Behaviors of Neuropathic Pain Model Rats Possibly via Inhibiting JAK2/STAT3

  • Li, Donghua (Department of Pain, Jinan Central Hospital Affiliated to Shandong University) ;
  • Yan, Yurong (Department of Anesthesiology, Binzhou Medical University Hospital) ;
  • Yu, Lingzhi (Department of Pain, Jinan Central Hospital Affiliated to Shandong University) ;
  • Duan, Yong (Department of State-owned Assets Management, Binzhou Medical University Hospital)
  • Received : 2016.01.07
  • Accepted : 2016.05.11
  • Published : 2016.09.01
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Abstract

Neuropathic pain (NPP) is the main culprit among chronic pains affecting the normal life of patients. Procaine is a frequently-used local anesthesia with multiple efficacies in various diseases. However, its role in modulating NPP has not been reported yet. This study aims at uncovering the role of procaine in NPP. Rats were pretreated with procaine by intrathecal injection. Then NPP rat model was induced by sciatic nerve chronic compression injury (CCI) and behavior tests were performed to analyze the pain behaviors upon mechanical, thermal and cold stimulations. Spinal expression of Janus kinase 2 (JAK2) and signal transducer and activator of transcription 3 (STAT3) was detected by qRT-PCR and western blot. JAK2 was also overexpressed in procaine treated model rats for behavior tests. Results showed that procaine pretreatment improved the pain behaviors of model rats upon mechanical, thermal and cold stimulations, with the best effect occurring on the $15^{th}$ day post model construction (p<0.05). Procaine also inhibited JAK2 and STAT3 expression in both mRNA (p<0.05) and protein levels. Overexpression of JAK2 increased STAT3 level and reversed the improvement effects of procaine in pain behaviors (p<0.01). These findings indicate that procaine is capable of attenuating NPP, suggesting procaine is a potential therapeutic strategy for treating NPP. Its role may be associated with the inhibition on JAK2/STAT3 signaling.

Keywords

References

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