• 한국산업정보학회논문지
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• 제19권3호
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• pp.17-23
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• 2014

Recently, E-Commerce demands an integrated solution for online and off-line provided to customers on wired / wireless network and various mobile devices. Our E-commerce platform can use anytime, anywhere, for all consumers. And, it can take convenient and secure E-commerce service in mobile environment. So, we will implement a software platform base on the mobile environment that independent of the mobile OS. Particularly, our E-commerce platform is based on 3D GIS information for 3D electronic map. So, all users (customers) have got the more visible convenience. And, by using the e-catalog DB cloud server, off-line store operators can be built the online home-page easily. From now on our E-commerce platform based on 3D GIS can be usable at various E-commerce areas in the ubiquitous environment.

• 한국응용곤충학회지
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• 제47권2호
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• pp.141-147
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• 2008

톱다리개미허리노린재(Riptortus clavatus Thunberg) (Heteroptera: Alydidae) 수컷 성충은 (E)-2-hexenyl (Z)-3-hexenoate (E2HZ3H), (E)-2-hexenyl (E)-2-hexenoate (E2HE2H) 및 tetradecyl isobutyrate(TI)의 세 성분으로 조성되는 집합페로몬을 분비하고, 그 페로몬에 동종의 암수 성충과 약충을 유인되며, 난기생봉인 Ooencyrtus nezarae (Hymenoptera: Encyrtidae)는 이 페로몬을 기주탐색에 이용한다. 본 연구에서는 한국의 진주와 익산, 일본의 Tsuhba와 Kumamoto지역에서 채집한 톱다리개미허리노린재의 집합페로몬 성분의 조성비를 조사하고, 각 성분의 배합비율별 유인력을 진주와 Kumamoto에서 조사하였다. 진주와 익산계통의 집합페로몬 조성 비율(E2HZ3H:E2HE2H:TI)은 각각 1:1.4:0.2, 1:0.8:0.2였으며, Tsuhba와 Kumamoto계통은 각각 1:2.8:0.2, 1:1.5:0.1이었다. 야외 유인력 검정에서, 1:1:1 (E2HZ3H:E2HE2H:TI=16.7:16.7:16.7mg/고무격막) 또는 1:1:0.5 (E2HZ3H:E2HE2H:TI=20:20:10mg/고무격막)로 배합한 페로몬이 1:5:1 (E2HZ3H:E2HE2H:TI = 7.1:35.7:7.1mg/고무격막)로 배합한 것보다 유인력이 높았다.

• Journal of Microbiology and Biotechnology
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• 제11권4호
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• pp.592-597
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• 2001

The pyruvate dehydrogenase complex (PDC) catalyzes the oxidative decarboxylation of pyruvate with the formation of $CO_2$, acetyl-CoA, NADH, and H+. This complex contains multiple copies of three catalytic components including pyruvate dehydrogenase(E1), dihydrolipoamide acetyltransferase(E2), and dihydrolipoamide dehydrogenase (E3). Two regulatory components (E1-kinase and phospho-E1 phosphatase) and functionally less-understood protein (protein X, E3BP) are also involved in the formation of the complex. In this study, cloning and characterization of a gene for human E3BP have been carried out. A cDNA encoding the human E3BP was isolated by database search and cDNA library screening. The primary structure of E3BP has some similar characteristics with that of E2 in the lipoyl domain and the carboxyl-terminal domain, based on the nucleotide sequence and the deduced amino acid sequence. However, the conserved amino acid moiety including the histidine residue for acetyltransferase activity in E2 is not conserved in the case of human E3BP. The human E3BP was expressed and purified in E. coli. The molecular weight of the protein, excluding the mitochondrial target sequence, was about 50 kDa as determined by SDS-PAGE. Cloning of human E3BP and expression of the recombinant E3BP will facilitate the understanding of the role(s) of E3BP in mammalian PDC.

• Journal of Microbiology and Biotechnology
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• 제20권9호
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• pp.1283-1287
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• 2010

In eukaryotes, eRF3 participates in translation termination and belongs to the superfamily of GTPases. In this work, the dissociation constants for nucleosides bound to Euplotes octocarinatus eRF3 in the presence and absence of eRF1a were determined using fluorescence spectra methods. Furthermore, a GTP hydrolyzing assay of eRF3 was carried out using an HPLC method, and the kinetic parameters for GTP hydrolysis by eRF3 were determined. Consistent with data from humans, the results showed that eRF1a promoted the binding of GTP to eRF3 and the GTP hydrolyzing activity of eRF3. However, in contrast to the lack of GTP binding in the absence of eRF1 in human eRF3, the E. octocarinatus eRF3 was able to bind GTP by itself. The nucleotide binding affinity of the E. octocarinatus eRF3 also differed from the human data. A structure model and amino acid sequence alignment of potential G domains indicated that these differences may be due to valine 317 and glutamate 452 displacing the conserved glycine and lysine involved in GTP binding.

• 한국정보통신학회논문지
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• 제7권4호
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• pp.638-645
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• 2003

인터넷상에서 서비스되고 있는 E-Mail 서비스는 POP3 또는 IMAP4 통신 규약을 기반으로 하고 있으며, 최근에는 이미지와 Hypertext를 포함한 메일 내용을 전송하고, 수신하는 기능이 보편화되고 있다. 무선 인터넷을 이용한 모바일 전자우편 서비스를 위해서는, POP3의 경우 전용 모바일 E-Mail 서버를 이용하거나 E-Mail 내용을 모바일에 적합하도록 변환하여야만 하고, Web-Mail로 이용되는 IMAP4 E-Mail의 경우에는 원격 파일 서버와 같이 서버 측에 E-Mail이 보관되므로 IMAP4 규약이 없는 모바일 클라이언트로는 모바일 E-Mail 서비스의 이용이 곤란하다. 특히, 이미지나 Hypertext를 포함하는 경우 이들이 문자형태로 전송되므로 모바일에서 정보로 인식하기 매우 곤란하다는 문제점이 있다. 본 논문에서는 최근 보편화되고 있는 IMAP4 기반의 Web-Mail을 모바일에서 이용할 수 있도록 하는 모바일용 E-Mail 추출 엔진을 구현함으로써, POP3 E-Mail 서비스뿐만 아니라 IMAP4를 기반으로 하는 Web-Mail을 모바일 환경에서 이용할 수 있도록 하였으며, 이미지 변환 기능을 설계하여 기존 E-Mail 서비스에서 제공하지 않았던 E-Mail에 포함된 이미지 정보를 사용자에게 제공하도록 하였다. 이와 같이 E-Mail 추출엔진과 이미지 변환 엔진을 설계 및 구현함으로써 모바일 환경에서 최적의 E-Mail서비스를 받을 수 있도록 하였다.

• 한국수정란이식학회지
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• 제28권3호
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• pp.215-222
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• 2013

Bovine coat color is decided by the melanocortin receptor 1 (MC1R) genotype mutation and melanogenesis. Specially, in the various cattle breeds, dominant black coat color is expressed by dominant genotype of $E^D$, red or brown is expressed in the frame shift mutation of recessive homozygous e by base pair deletion and wild type of $E^+$ is expressed in various coat colors. However, not very well known about the effected of MC1R genotype mutation on the coat color through family lines in KBC. Therefore, this study were to investigate effect of MC1R genotype mutation on the coat color, and to suggest mating breed system in accordance with of MC1R genotype for increased on brindle coat color appearance. Parents (sire 2 heads and dam 3 heads) and offspring (total : 54 heads) from crossbreeding in KBC family line with the MC1R genotype and phenotype records were selected as experimental animals. The relationship between melanocortin 1 receptor (MC1R) genotypes expression verified by PCR-RFLP, and brindle coat color appearance to the family line of the cross mating breed from MC1R genotype pattern was determined. As a result, 4MC1R genetic variations, $E^+/E^+$ (sire 1), $E^+/e$ (sire 2 and dam 3), $E^+/e$ with 4 bands of 174, 207 and 328 bp (dam 1) and $E^+/e$ with 3 bands of 174, 207, 328 and 535 bp (dam 2) from parents (sire and dam) of KBC. However, 3 genetic variations, e/e (24%), $E^+/E^+$ (22%) and $E^+/e$ (56%) were identified in offspring. Also, brindle coat color expressrated was the e/e with the 0%, $E^+/E^+$ with 67% and $E^+/e$ with 77% from MC1R genotype in offspring on the cross mating of KBC. Furthermore, when the sire had $E^+/e$ genotype and the dam had $E^+/E^+$ with the 3 bands or $E^+/e$ genotype, and both had whole body-brindle coat color, 62% of the offspring had whole body-brindle coat color. Therefore, the seresults, the mating system from MC1R genotype patterns of the sires ($E^+/e$) and dams ($E^+/E^+$ with the 3 bands or $E^+/e$) with brindle coat color may have the highest whole body-brindle coat color expression in their offspring.

• 한국멀티미디어학회:학술대회논문집
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• 한국멀티미디어학회 2003년도 춘계학술발표대회논문집
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• pp.628-634
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• 2003

인터넷상에서 서비스되고 있는 E-Mail 서비스는 POP3 또는 IMAP4 통신 규약을 기반으로 하고 있으며, 최근에는 이미지와 Hypertext를 포함한 메일 내용을 전송하고, 수신하는 기능이 보편화되고 있다. 무선 인터넷을 이용한 모바일 전자우편 서비스를 위해서는, POP3의 경우 전용 모바일 E-Mail 서버를 이용하거나 E-Mail 내용을 모바일에 적합하도록 변환하여야만 하고, Web-Mail로 이용되는 IMAP4 E-Mail의 경우에는 원격 파일 서버와 같이 서버 측에 E-Mail이 보관되므로 IMAP4 규약이 없는 모바일 클라이언트로는 모바일 E-Mail 서비스의 이용이 곤란하다 특히, 이미지나 Hypertext를 포함하는 경우 이들이 문자형태로 전송되므로 모바일에서 정보로 인식하기 매우 곤란하다는 문제점이 있다. 본 논문에서는 최근 보편화되고 있는 IMAP4 기반의 Web-Mail을 모바일에서 이용할 수 있도록 하는 모바일용 E-Mail 추출 엔진을 구현함으로써, POP3 E-Mail 서비스뿐만 아니라 IMAP4를 기반으로 하는 Web-Mail을 모바일 환경에서 이용할 수 있도록 하였으며, 이미지 변환 기능을 설계하여 기존 E-Mail 서비스에서 제공하지 않았던 E-Mail에 포함된 이미지 정보를 사용자에게 제공하도록 하였다. 이와 같이 E-Mail 추출엔진과 이미지 변환 엔진을 설계 및 구현함으로써 모바일 환경에서 최적의 E-Mail 서비스를 받을 수 있도록 하였다.

• 대한바이러스학회지
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• 제27권1호
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• pp.9-17
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• 1997

Hepatitis C virus (HCV) E2 protein is known to be one of putative envelope proteins. To develop a sensitive detection method for HCV infected tissues and cells, monoclonal antibodys (MAbs) to the E2 protein of HCV were prepared from mice immunized with recombinant baculovirus-expressing E2 protein (Bac-E2). Several hybridoma clones secreting various levels of MAb were isolated and isotypes of these MAb were determined. One clone (L.2.3.3) was used for ascites production and the E2-MAb was purified and characterized. The L.2.3.3 reacted well with both Bac-E2 and E. coli expressed glutathione-S-transferase-E2 (GST-E2) fusion proteins. Using HCV patient sera, E2 envelope protein was found to be localized in the cell membrane boundary both in CHO cells and insect cells which express HCV E2 protein. Similar result was obtained when same cells were treated with the MAb L.2.3.3. These results demonstrated that Bac-E2 protein is capable of eliciting high titer antibody production in mice.

• Fisheries and Aquatic Sciences
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• 제13권2호
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• pp.118-126
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• 2010

A feeding trial was conducted to investigate the effects of different levels of dietary n-3 highly unsaturated fatty acids (HUFA) (1.1-5.6%) and vitamin E (70 and 400 mg/kg) on the growth and body composition of juvenile rockfish. Six isonitrogenous (45% crude protein) and isolipidic (17% crude lipid) diets were formulated to contain graded levels of n-3 HUFA and vitamin E. Diets 1, 2 and 3 consist of 400 mg vitamin E/kg diet with graded levels of 1.1, 3.0, and 5.6% n-3 HUFA, respectively. Graded levels of n-3 HUFA (1.1, 3.0, and 4.0%) were added in diets 4, 5 and 6, respectively, containing 70 mg vitamin E/kg diet each. At the end of feeding trial, growth performance of rockfish was affected by neither dietary n-3 HUFA nor vitamin E levels. Feed efficiency and hepatosomatic index were slightly decreased (P<0.05) with increment of dietary n-3 HUFA at each dietary vitamin E level. Dietary vitamin E and n-3 HUFA levels did not affect proximate composition and vitamin E concentration in the dorsal muscle of rockfish. Liver moisture and crude protein contents positively related to dietary n-3 HUFA levels. Liver lipid content and hematocrit value were significantly decreased (P<0.05) by increasing dietary n-3 HUFA levels. Eicosapentaenoic acid (20:5n-3; EPA) and docosahexaenoic acid (22:6n-3; DHA) concentrations in the dorsal muscle significantly correlated to dietary n-3 HUFA levels, except for fish fed the diet 6 containing 4% n-3 HUFA and 70 mg vitamin E/kg diet. EPA concentration in the dorsal muscle of fish fed the diet 6 was significantly lower than that of fish fed the diets 2, 3 and 5. The present findings suggest that feeding of diets containing excessive n-3 HUFA level with varying addition of vitamin E may alter fatty acid composition in the dorsal muscle, but do not affect growth of juvenile rockfish.

• 대한수학회논문집
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• 제27권3호
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• pp.497-503
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• 2012

In this paper, we study elliptic curves E over $\mathbb{Q}$ such that the 3-torsion subgroup E[3] is split as ${\mu}_3{\oplus}\mathbb{Z}/3{\mathbb{Z}}$. For a non-zero intege $m$, let $C_m$ denote the curve $x^3+y^3=m$. We consider the relation between the set of integral points of $C_m$ and the elliptic curves E with $E[3]{\simeq}{\mu}_3{\oplus}\mathbb{Z}/3{\mathbb{Z}}$.