• 대한방사선기술학회지:방사선기술과학
• /
• 제43권1호
• /
• pp.9-14
• /
• 2020

Commercial plate bolus is generally used for treatment of surface tumor and required surface dose. We fabricated 3D-printed bolus by using 3D printing technology and usability of 3D-printed bolus was evaluated. RT-structure of contoured plate bolus in the TPS was exported to DICOM files and converted to STL file by using converting program. The 3D-printed bolus was manufactured with rubber-like translucent materials using a 3D printer. The dose distribution calculated in the TPS and compared the characteristics of the plate bolus and the 3D printed bolus. The absolute dose was measured inserting an ion chamber to the depth of 5 cm and 10 cm from the surface of the blue water phantom. HU and ED were measured to compare the material characteristics. 100% dose was distributed at Dmax of 1.5 cm below the surface when was applied without bolus. When the plate bolus and 3D-plate bolus were applied, dose distributed at 0.9 cm and 0.8 cm below the surface of the bolus. After the comparative analysis of the radiation dose at the reference depth, differences in radiation dose of 0.1 ~ 0.3% were found, but there was no difference dose. The usability of the 3D-printed bolus was thus confirmed and it is considered that the 3D-printed bolus can be applied in radiation therapy.

• 대한한의학회지
• /
• 제22권4호
• /
• pp.29-36
• /
• 2001

Objectives: This study attempted to investigate the allergic histories and the distribution of offending allergens in the general public and further to help their diagnosis and treatment with Oriental Medicine. Methods: Allergic skin tests (AST) were performed and allergic histories were taken of 359 members of the general public who visited the International Exhibition on Oriental Medicine from Sept. 1 to Sept. 5, 2000. The allergen reagents for AST were three (House dust, D. farinae, Dog hair) and the control reagent was histamine solution. Results: 1.50.1 % of the subjects (n=359) were positive to AST. The ratio between males' positivity and females' was 1.06：1. 2. The younger the subjects were, the higher the positivity was. 3. The positive subjects' (n=180) positivity to three allergens was as follows: D. farinae 98.9%, House dust 30.0%, Dog hair 1.7%. 4. The younger the positive subjects were, the higher the positivity to House dust was. In contrast, the positivity to D. farinae was high in all age groups. 5.71.1 % of the positive subjects reacted positively to monotype allergen and 28.9% reacted positively to multiple allergens (2.46：1). The most common monotype allergen was D. farinae (98.4%) and the most common combination of multiple allergens was House dust and D. farinae (94.2%). 6.52.8% of the positive subjects (n=180) and 51.4% of the negative subjects (n=179) represented the history of allergic diseases. There was no relationship between allergic skin test and allergic diseases. Conclusion: To help in diagnosis and treatment with oriental medicine, research to analyze the relationship between allergic skin test and allergic diseases should be continued on the basis of Oriental medical theories.

• Biomolecules & Therapeutics
• /
• 제31권6호
• /
• pp.682-691
• /
• 2023

Cell transformation induced by epidermal growth factor (EGF) and 12-O-tetradecanoylphorbol-13-acetate (TPA) is a critical event in cancer initiation and progression, and understanding the underlying mechanisms is essential for the development of new therapeutic strategies. Licorice extract contains various bioactive compounds, which have been reported to have anticancer and anti-inflammatory effects. This study investigated the cancer preventive efficacy of licochalcone D (LicoD), a chalcone derivative in licorice extract, in EGF and TPA-induced transformed skin keratinocyte cells. LicoD effectively suppressed EGF-induced cell proliferation and anchorage-independent colony growth. EGF and TPA promoted the S phase of cell cycle, while LicoD treatment caused G1 phase arrest and down-regulated cyclin D1 and up-regulated p21 expression associated with the G1 phase. LicoD also induced apoptosis and increased apoptosis-related proteins such as cleaved-caspase-3, cleaved-caspase-7, and Bax (Bcl2-associated X protein). We further investigated the effect of LicoD on the AKT signaling pathway involved in various cellular processes and found decreased p-AKT, p-GSK3β, and p-NFκB expression. Treatment with MK-2206, an AKT pharmacological inhibitor, suppressed EGF-induced cell proliferation and transformed colony growth. In conclusion, this study demonstrated the potential of LicoD as a preventive agent for skin carcinogenesis.

• 약학회지
• /
• 제54권2호
• /
• pp.112-121
• /
• 2010

The cytotoxic activity of 33,4,5-trihydroxybenzoic acid methylester and related compounds on the growth of normal cell lines, human skin melanoma cells and human oral epithelioid cell line were evaluated by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and 2,3-bis-[2-methoxy-4-nitro-5-sulfo-phenyl]-2-H-tetrazolium-5-caboxanilide (XTT) methods. 3,4,5-Trihydroxybenzoic acid methylester decreased the cell viability of human skin melanoma cells and human oral epithelioid cells shown by the MTT method and the cell adhesion activity of human skin melanoma cells and human oral epithelioid cells shown by the XTT method. In light microscopy, 100 ${\mu}M$ 3,4,5-trihydroxybenzoic acid methylester showed the highest cytotoxic activity. These results suggest that 3,4,5-trihydroxybenzoic acid methylester has a potential anticancer activity.

• 폴리머
• /
• 제33권3호
• /
• pp.237-242
• /
• 2009

알렌드로네이트의 생체이용률을 높이고 경구복용시 발생하는 부작용을 해소하고자 경피약물전달시스템에 전기이온영동법, microneedle 등을 적용하여 in-vitro 시험 후 약물전달량을 HPLC-Flu를 이용하여 조사하였다. 전기이온영동시험을 위해 사용된 약물 패취는 UV중합법으로 합성하였으며, 이때 패취에 함유된 알렌드로네이트의 량은 $5.0\;mg/cm^3$이었다. 0.25, $0.50\;mA/cm^2$의 전류를 인가한 경우, 약물전달량은 각각 $0.80{\pm}0.03$와 $2.00{\pm}0.02{\mu}g$이었다. microneedle로 전처리 후의 전달량은 각각 $70.65{\pm}0.37$와 $162.23{\pm}0.40{\mu}g$으로 증가했다. 경피약물전달용 알렌드로네이트 패취의 생체적 합성 평가는 ISO 10993에 따라 시험하였다.

• Toxicological Research
• /
• 제35권4호
• /
• pp.353-359
• /
• 2019

The purpose of this study was to investigate the effect of $7-MEGA^{TM}$ 500 on the improvement of skin aging in an UVB-induced photo-aging model of hairless mice. The dorsal skin of hairless mice was exposed to UVB three times a week for 12 weeks to induce skin wrinkle. After inducing the wrinkle, $7-MEGA^{TM}$ 500 was orally administered once a day for 4 weeks. Skin thickness, skin barrier function, and wrinkle indicators were improved by treatment with $7-MEGA^{TM}$ 500. Both gene and protein expression levels of MMP-3 and c-Jun in skin were significantly decreased by $7-MEGA^{TM}$ 500. Therefore, the intake of $7-MEGA^{TM}$ 500 is thought to have a positive effect on the improvement of skin aging, although further studies are needed.

• 대한본초학회지
• /
• 제24권2호
• /
• pp.1-5
• /
• 2009

Objectives : HRHDT has been known as a useful prescription with antibiotic, anti-inflammatory, antioxidative and immunosuppressive activity. To evaluate anti-inflammatory effect of HRHDT, we treated HRHDT-skin in Balb/c mice model induced contact hypersensitivity. Methods : Contact hypersensitivity, a local inflammatory respinse of skin, was induced by spreading the back skin of Balb/c mice with 1% DNCB. HRHDT-skin was prepared by dissolving 3% 1,3-butylene glycol extract of HRHDT in solution and treated 2 weeks on the back skin. Results: HRHDT-skin significantly reduced TEWL and erythema by 0.4-1% of DNCB treatment compared with control group. HRHDT-skin reduced IgE on serum obtained from blood of DNCB-treated Balb/c mice. Histopathological examination showed that thickening of the epidermis, hyperkeratosis and the infiltration of inflammatory cells were found in Balb/c mice under conventional circumstances. Conclusions : These results showed that HRHDT-skin could be used as a pharmaceutical material with antiinflammatory effects by reducing IgE in contact hypersensitivity dermatitis Balb/c mice by DNCB.

• 한국정밀공학회:학술대회논문집
• /
• 한국정밀공학회 2010년도 춘계학술대회 논문집
• /
• pp.995-996
• /
• 2010

• 대한화장품학회지
• /
• 제40권2호
• /
• pp.195-201
• /
• 2014

생체수는 링거액, 인공관절액, 세포 배양 등 다양한 분야에서 연구되어 왔다. 이는 생체수가 체온을 조절하는데 결정적인 역할을 하고, 생체 내 다양한 대사 과정에서 용매로 사용되며, 삼투압이나 능동적 섭취를 통한 혈액 또는 림프액을 통해 세포에서 세포로 전달되는 미네랄, 에너지원, 호르몬, 시그널과 약물의 전달 물질로 이용되기 때문이다. 세포외 지질과 자연보습성분(natural moisturizing factor, NMF)을 함유하는 각질층은 외부의 수분을 끌어들여 내부 수화에 이용하며, 이러한 과정들은 피부 장벽 기능과의 연관성이 높다. 본 연구에서는 피부 장벽 기능을 강화하는 아미노산, 펩타이드, 단당류를 포함한 생체모사수(Cell Bio Fluid SyncTM)를 인체 피부에 처리하여 세포활성을 관찰하고, 생체 모사수를 함유한 제품을 피부에 도포하여 인체피부 개선 효과를 연구 하였다. 피부 세포 활성을 보기 위해 각질세포인 HaCaT 세포를 이용하였고, 에너지 고갈상태로 만들기 위해 3시간 동안 PBS에 전 처리한 후 이후 세포 배양액인 DMEM 및 등장액인 PBS, 생체 모사수(Cell Bio Fluid $Sync^{TM}$)에 각각 3시간 처리하였다. 이후 MTT assay와 이미지 분석을 수행하였다. 인체의 피부 개선 효능을 위한 임상 연구에 21명의 여성이 참여하였다. 생체 모사수를 함유한 제품을 1주일간 안면에 도포하였고, 수분량, 피부결, 밝기 그리고 피부 균일도를 측정하였다. 모든 측정은 피험자가 세안 후 항온항습조건($22{\pm}2^{\circ}C$, $50{\pm}5%$)에서 20분 간 대기하고 수행하였다. 모든 데이터는 SPSS ver. 21을 이용하여 분석하였다. 그 결과, 생체 모사수(Cell Bio Fluid $Sync^{TM}$)가 세포 활성을 높이고 인체의 피부에서 수분, 거칠기, 밝기, 균일도, 투명도 등의 개선 효과가 있음을 확인할 수 있었다.

• 대한화장품학회지
• /
• 제31권1호
• /
• pp.97-102
• /
• 2005

수분 보유력이 우수한 hyaluronan (HA)은 피부 보습에 관여하는 여러 물질들 중 하나로 피부의 extracellular matrix를 구성하는 주요 성분 중 하나이다. Glycosaminoglycans (GAGs)의 구성 성분의 하나로 과거에는 진피에서 유래하는 것으로 알려져 왔으나 최근 연구들을 통해 표피에서 합성되는 것이 확인되었다. Polyphenolic compound의 일종인 kaempferol과 quercetin은 채소류 같은 식물성 음식에 많이 존재하는 것으로 알려져 있으며, kaempferol은 인체 표피세포에서 glutathione 합성을 증가시키고 quercetin은 lipoxygenase inhibitor로 PPAR (peroxisome proliferator activated receptor) - mediated 표피세포 분화를 억제하는 것으로 알려져 있다. 본 연구에서 우리는 표피 세포주에서 이들 flavonoids -kaempferol, quercetin -의 HA 합성에 미치는 영향을 알아보고자 하였다. 물질 처리에 따른 HA 합성 효소인 hyaluronan synthase 1, 2, 3 (HAS1, 2, 3) 유전자 발현의 변화를 semi-quantitative RT-PCR을 통해 살펴보았다. 이들 flavonoid들에 의해 24 h 후 HAS2, 3 mRNA 발현이 증가되는 것을 발견하였다. 또한 HA 합성량의 변화를 알아보기 위해 ELISA를 수행하였다. 24 h 물질 처리 후 배지를 수거하여 HA 합성량을 살펴본 결과 이들 물질에 의해 합성이 유의하게 증가함을 알 수 있었다. 비록 합성 촉진에서의 효과가 retinoic acid에는 못 미치지만 kaempferol과 quercetin은 표피 세포주에서 농도 의존적으로 HA 합성을 증가시켰다. 위의 결과를 통해 flavonoid류인 kaempferol과 quercetin이 피부에서 HA 생산을 촉진시킴을 알 수 있었고 이를 통해 피부 보습과 잔주름 개선에 효과를 볼 수 있을 것으로 생각된다.