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The Cytotoxic Activity of 3,4,5-Trihydroxybenzoic Acid Methylester and Related Compounds against Skin and Oral Cancer Cell Lines  

Lee, Jae-Sug (Department of Beauty Science, Kwangju Wonmen's University)
Han, Du-Seok (Department of Oral Anatomy, School of Dentistry, Wonkwang University)
Kang, Jeong-Il (Department of Physical Therapy, Daebul University Youngam)
Baek, Jong-Min (R&D Center, BRNScience Co. LTD., Seoul National University)
Baek, Seung-Hwa (Department of Herbal Resources, Professional School of Oriental Medicine, Wonkwang University)
Publication Information
YAKHAK HOEJI / v.54, no.2, 2010 , pp. 112-121 More about this Journal
Abstract
The cytotoxic activity of 33,4,5-trihydroxybenzoic acid methylester and related compounds on the growth of normal cell lines, human skin melanoma cells and human oral epithelioid cell line were evaluated by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and 2,3-bis-[2-methoxy-4-nitro-5-sulfo-phenyl]-2-H-tetrazolium-5-caboxanilide (XTT) methods. 3,4,5-Trihydroxybenzoic acid methylester decreased the cell viability of human skin melanoma cells and human oral epithelioid cells shown by the MTT method and the cell adhesion activity of human skin melanoma cells and human oral epithelioid cells shown by the XTT method. In light microscopy, 100 ${\mu}M$ 3,4,5-trihydroxybenzoic acid methylester showed the highest cytotoxic activity. These results suggest that 3,4,5-trihydroxybenzoic acid methylester has a potential anticancer activity.
Keywords
cytotoxic activity; NIH 3T3 fibroblasts; MTT and XTT methods; 3,4,5-trihydroxybenzoic acid methylester; human oral epithelioid cells; human skin melanoma cells;
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