• Current Research on Agriculture and Life Sciences
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• v.31 no.1
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• pp.30-37
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• 2013

Bacillus subtilis and Bacillus amyloliquefaciens are closely related species that share a similar genomic background, and are both known to secrete large amounts of proteins directly into a medium. The extracellular proteomes of two strains of Bacillus subtilis and two strains of Bacillus amyloliquefaciens were compared by 2-D gel electrophoresis during the late exponential growth phase. The relative abundance of some minor protein spots varied among the four strains of Bacillus. Over 123 spots of extracellular proteins were visualized on the gel for B. subtilis CH 97, 68 spots for B. subtilis 3-5, 230 spots for B. amyloliquefaciens CH 51, and 60 spotsfor B. amyloliquefaciens 86-1. 2D gel electrophoresis images of the four Bacillus strains showed significantly different protein profiles. Consistent with the 2D gel electrophoretic analysis, most of the B. subtilis proteins differed from the proteases secreted by the B. amyloliquefaciensstrains. Among the proteins identified from B. subtilis, approximately 50% were cytoplasmic and 30% were canonically extracellular proteins. The secreted protein profiles for B. subtilis CH 97 and B. subtilis 3-5 were quite different, as were the profiles for B. amyloliquefaciens CH 51 and 86-1. The four proteomes also differed in the major protein composition. The B. subtilis CH 97 and B. amyloliquefaciens CH 51 proteomes both contained large amounts of secreted hydrolytic enzymes. Among the four strains, B. subtilis 3-5 secreted the least number of proteins. Therefore, even closely related bacteria in terms of genomic sequences can still have significant differences in their physiology and proteome layout.

• Proceedings of the IEEK Conference
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• 2006.06a
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• pp.475-476
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• 2006

Recently, the demand for high density MOSFET arrays are increasing. In implementing 3-D devices to this end, it is inevitable to ion-implant vertically in order to avoid screening effects caused by high silicon fins. In this study, the dependency of drain current characteristics on doping profiles is investigated by 3-D numerical analysis. The position of concentration peak (PCP) and the doping gradient are varied to look into the effects on primary current characteristics. Through these analyses, criteria of ion-implantation for 3-D devices are established.

• Journal of the Korean Institute of Gas
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• v.26 no.5
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• pp.58-65
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• 2022

Gas explosion accidents could cause a catastrophe. we need specialized and systematic accident investigation techniques to shed light on the cause and prevent similar accidents. In this study, we had performed LPG explosion simulation using AUTODYN which is the commercial explosion program and predicted the damage characteristics of the structures by LNG explosive power. In the first step, we could get LPG's physical and chemical explosion properties by calculation using TNT equivalency method. And then, by applying TNT equivalency value about the explosion limit concentration of LPG on the 2D-AUTODYN simulation, we could get the explosion pressure wave profiles (explosion pressure, explosion velocity, etc.). In the last step, we performed LPG explosion simulation by applying to the explosion pressure wave profiles as the input data on the 3D-AUTODYN simulation. As a result, we had performed analyzing of the explosion characteristics of LPG in accordance with concentration through the 3D-AUTODYN simulation in terms of the explosion pressure behavior and structure destruction and damage behavior. The analyses showed that the generated stresses of the structures were lower than the compressive strengths in cases 1(two lane) and 2(four lane), while the generated stress in case 3(six lane) was 8.68e3 kPa, which exceeded the compressive strength of 5.89e3 kPa.

• The Bulletin of The Korean Astronomical Society
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• v.42 no.2
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• pp.61.3-61.3
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• 2017

We have studied the high-resolution $H{\alpha}$ and Ca II $8542{\AA}$ line profiles of Ellerman Bombs (EBs) obtained with the Fast Imaging Solar Spectrograph (FISS) installed on the 1.6m Goode Solar Telescope (GST) in Big Bear Solar Observatory (BBSO). The FISS spectra of EBs are compared with synthetic profiles computed using RH non-LTE radiative transfer code and a set of 1D atmospheric models with local transient heating, the latter of which is modeled by varying local temperature enhancement in magnitude and height. We could reproduce each of the observed $H{\alpha}$ and Ca II line profiles separately with different atmospheric models, but not with a single atmospheric model. To fit the observed $H{\alpha}$ lines we often need much higher temperature enhancements than those needed for fitting Ca II lines. Possible causes for this temperature mismatch are briefly discussed.

• Journal of Korean Vacuum Science & Technology
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• v.6 no.1
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• pp.5-7
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• 2002

For quantitative N depth profiling, N profiles were measured in a～3 m Si oxynitride by low energy O$\sub$2+/sputtering and the result was calibrated with MEIS analysis of the N thickness and areal density. The quantitative depth profile of nitrogen showed the pileup of nitrogen atoms at the interface of ultrathin oxynitride films.

• Journal of The Korean Astronomical Society
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• v.3 no.1
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• pp.1-5
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• 1970

The earlier findings on the radiative heating through the umbral walls in large sunspots are further investigated. No significant evidence for the umbra! heating has been found in small-sized sunspot umbrae.

• Journal of Institute of Control, Robotics and Systems
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• v.19 no.11
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• pp.968-973
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• 2013

In the transportation industry, especially in the shipbuilding process, 3D surface measurement of large-scale hull pieces is needed for fabrication and assembly. We suggest an efficient method for checking the shape of curved plates under the forming operation with short time by measuring 3D profiles along the multi lines of the target surface. For accurate profile reconstruction, 2D camera calibration and 3D calibration using gauge blocks were performed. The evaluation test shows that the measurement accuracy is within the boundary of tolerance required in the shipbuilding process.

• Journal of Nutrition and Health
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• v.49 no.1
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• pp.28-35
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• 2016

Purpose: Vitamin D status is associated with several chronic diseases related to obesity. In this study, we evaluate the nutritional status of vitamin D and its relation to obesity indices in Korean women. Methods: A total of 156 healthy women participated. Vitamin D status (serum $25-OH-vitamin\;D_3$ level) and obesity indices (body mass index, body fat mass, waisthip ratio, and body fat percentage etc.) and serum lipid profiles and serum adipokine (leptin and adiponectin) levels were analyzed. Results: The $25(OH)D_3$ level showed an extremely skewed distribution from 4.1 ng/ml to 24.4 ng/ml and mean $25(OH)D_3$ level was $9.0{\pm}4.0ng/ml$. With cut-off level for vitamin D deficiency (< 12.0 ng/ml), insufficiency (12-19.9 ng/ml) and sufficiency (${\geq}20ng/ml$), 77.6%, 19.2%, and 3.2% of subjects showed vitamin D deficiency, insufficiency, and sufficiency status, respectively. The $25(OH)D_3$ level showed positive correlation with weight (r = 0.2461, p < 0.01), body mass index (r = 0.2913, p < 0.001), body fat contents (r = 0.1691, p < 0.05), fat free mass (r = 0.2330, p < 0.01), and waist hip ratio (r = 0.1749, p < 0.05) after adjusted by age. The $25(OH)D_3$ level showed no significant correlation with serum lipid profiles and adipokine levels. Conclusion: Most subjects (76.6%) in this study, who had a vitamin D deficient status and serum $25(OH)D_3$ level, showed positive correlation with several obesity indices, however further research based on a large Korean population is needed to confirm the relationship.

• Structural Engineering and Mechanics
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• v.52 no.4
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• pp.663-686
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• 2014

This paper deals with free vibration analysis of bidirectional functionally graded annular plates resting on a two-parameter elastic foundation. The formulations are based on the three-dimensional elasticity theory. This study presents a novel 2-D six-parameter power-law distribution for ceramic volume fraction of 2-D functionally graded materials that gives designers a powerful tool for flexible designing of structures under multi-functional requirements. Various material profiles along the thickness and in the in-plane directions are illustrated by using the 2-D power-law distribution. The effective material properties at a point are determined in terms of the local volume fractions and the material properties by the Mori-Tanaka scheme. The 2-D differential quadrature method as an efficient and accurate numerical tool is used to discretize the governing equations and to implement the boundary conditions. The fast rate of convergence of the method is shown and the results are compared against existing results in literature. Some new results for natural frequencies of the plates are prepared, which include the effects of elastic coefficients of foundation, boundary conditions, material and geometrical parameters. The interesting results indicate that a graded ceramic volume fraction in two directions has a higher capability to reduce the natural frequency than conventional 1-D functionally graded materials.

• Journal of the Korean Association of Geographic Information Studies
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• v.15 no.4
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• pp.162-174
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• 2012

To visualize the characteristics of vertical seawater temperature data, in the ocean having 3D spatial characteristics, 2D thematic maps like horizontal seawater temperature distribution map at each depth layer and 3D volume model using 3D spatial interpolation are used. Although these methods are useful to understand oceanographic phenomena visually, there is a limit to analyze the spatial pattern of vertical temperature distribution or the relationship between vertical temperature characteristics and other oceanic factors (seawater chemistry, marine organism, climate change, etc). Therefore, this study aims to determine the spatial distribution characteristics of vertical temperature profiles in the South Sea of Jeju by quantifying the characteristics of vertical temperature profiles by using an algorithm that can extract the thermocline parameters, such as mixed layer depth, maximum temperature gradient and thermocline thickness. For this purpose spatial autocorrelation index (Moran's I) was calculated including mapping of spatial distribution for three parameters representing the vertical temperature profiles. Also, after grouping study area as four regions by using cluster analysis with three parameters, the characteristics of vertical temperature profiles were defined for each region.