• Applied Biological Chemistry
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• v.38 no.1
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• pp.63-66
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• 1995

Soybean nuclear extracts were prepared to detect SEF3(soybean embryo factor 3), which is presumed to be a trans-acting factor for the expression of the soybean ${\beta}-conglycinin\;{\alpha}'$ subunit gene. To increase the specific activity of DNA probe during labeling with $[{\alpha}-^{32}P]$dATP, dATP was added to a final concentration of 1.1 mM during the chase reaction. It results in approximately four-fold increase of specific activity of the DNA probe. Effects of several modifications in preparation of soybean nuclear extracts were examined. It was found that glycerol is effective to stabilize SEF3 during the preparation of nuclear extracts and polyethylenimine could be used to increase the specific activity of SEF3 in nuclear extracts.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.27 no.5
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• pp.416-423
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• 2016

In this paper, a conformal type $1{\times}4$ microstrip patch array antenna with tilted antenna beam is presented for UWB sensor applications. Each antenna element comprises E-shaped patch with L-probe feeding to increase the bandwidth. The tilted antenna beam of 20 degree can be achieved with 42 ps microstrip delay lines at the series feeding network with T-junctions. The measured reflection coefficient is >9 dB at 3.5~4.5 GHz. The measured antenna gain and HPBW are >10 dBi and <30 degree at 3.75~4.25 GHz.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.41 no.3
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• pp.139-146
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• 2004

A 2D/3D complex optical system and its vision inspection algerian is proposed and implemented as a single probe system for high speed, precise vision inspection of the solder pastes. One pass un length labeling algorithm is proposed instead of the conventional two pass labeling algorithm for fast extraction of the 2D shape of the solder paste image from the recent line-scan camera as well as the conventional area-scan camera, and the optical probe path generation is also proposed for the efficient 2D/3D inspection. The Moire interferometry-based phase shift algerian and its optical system implementation is introduced, instead of the conventional laser slit-beam method, for the high precision 3D vision inspection. All of the time-critical algorithms are MMX SIMD parallel-coded for further speedup. The proposed system is implemented for simultaneous 2D/3D inspection of 10mm${\times}$10mm FOV with resolutions of 10 ${\mu}{\textrm}{m}$ for both x, y axis and 1 ${\mu}{\textrm}{m}$ for z axis. Experiments conducted on several nBs show that the 2D/3D inspection of an FOV, excluding an image capturing, results in high speed of about 0.011sec/0.01sec, respectively, after image capturing, with $\pm$1${\mu}{\textrm}{m}$ height accuracy.

• Proceedings of the KIEE Conference
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• 2000.07d
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• pp.2849-2850
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• 2000

We developed an automatic 3D inspection system. The system consists of two parts one includes hardwares such as actuators. linear scales and a probe. etc. the other involves softwares for management and control of the system. Compared with existing 3D measurement systems. this system achieved automatically the inspection. This automatic inspection makes the system have some advantages to reduce a measurement time and to be easily used by operators.

• Proceedings of the KIEE Conference
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• 2001.07d
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• pp.2628-2630
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• 2001

This paper developed a 3D inspection system. The system consists of two parts : one includes hardwares such as actuators, linear scales and a probe, etc. the other involves softwares for management and control of the system. Compared with existing 3D measurement systems, this system achieved automatically the inspection. This automatic inspection makes the system have some advantages to reduce a measurement time and to be easily used by operators.

• Proceedings of the KIEE Conference
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• 1999.07g
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• pp.3070-3072
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• 1999

We developed an automatic 3D inspection system. The system consists of two parts : one includes hardwares such as actuators, linear scales and a probe, etc. the other involves softwares for management and control of the system. Compared with existing 3D measurement systems, this system achieved automatically the inspection. This automatic inspection makes the system have some advantages to reduce a measurement time and to be easily used by operators.

• Journal of the Korean Society for Precision Engineering
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• v.15 no.3
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• pp.99-106
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• 1998

Recently, scientists introduced a new type of microscope capable of investigating nonconducting surfaces in an atomic scale, which is called AFM (Atomic Force Microscope). It was an innovative attempt to overcome the limitation of STM (Scanning Tunnelling Microscope) which has been able to obtain the image of conducting surfaces. Surfaces of samples are imaged with atomic resolution. The AFM is an imaging tool or a profiler with unprecedented 3-D resolution for various surface types. The AFM technology, however, leaves a lot of room for improvement due to its delicate and fragile probing mechanism. One of the room for improvements is gap control between probe tip and sample surface. Distance between probe tip and sample surface must be kept in below one Angtrom in order to measure the sample surface in Angstrom resolution. In this paper, AFM system modeling, experimental system identification and control scheme based on system identification are performed and finally sample surface is measured by home-built AFM with such a control scheme.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.11 no.2
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• pp.217-227
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• 2000

A rectangular ceramic dielectric antenna, placed on the top of a ground plane and fed by coaxial probe, is analyzed by using the finite element method. To verify the proposed code, a rectangular ceramic dielectric antenna was fabricated and the characteristics of antenna were measured. The numerical data obtained is in good agreements with experimental result. Thus we checked the validity of our FEM code, and it can be possible to extend the analysis of the arbitrary 3-D antenna on a ground plane fed by coaxial probe.

• Journal of Ocean Engineering and Technology
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• v.26 no.3
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• pp.61-65
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• 2012

Air-water flow measurements are of importance for the coastal and ocean engineering fields. Although kinematic investigations of the multi-phase flows have been conducted for long time, velocity measurements still are concerned with many researchers and engineers in coastal and ocean areas. In the present study, an imaging technique using shadowgraphy and fiber optic probe for velocity measurements of air bubbles is introduced. The shadow graphy image technique is modified from the typical image velocimetry methods, and optical fibers are used for the well-known intrusive coupled phase-detection probe system. Since the imaging technique is a non-intrusive optical method from the air, it is usually applied for 2D flows. On the other hand, the double fiber optic probes touch flows regardless of flow patterns. The results of the flow measurements by both methods are compared and discussed. The methods are also applied to the measurements of overtopping flows by a breaking wave over the structure fixed on the free surface.

• Journal of Plant Biotechnology
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• v.31 no.4
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• pp.319-323
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• 2004

Soluble latex protein fraction excreted from Euporbia lathyris laticifer was resolved by 10% SDS-polyacrylamide gel electrophoresis to identify distinctively displayed latex major protein bands including ELp65, ELp55, ELp43, ELp32 and ELp23. Among them, ELp65 was purified by ammonium sulfate precipitation, gel permeation chromatography and ion exchange chromatography. Its N-terminal amino acid sequencing revealed its homology to the leading region of mature peptide of tomato p69a subtilisin-like protease, suggesting a certain role involved in plant defense system. In the analysis of Southern blot hybridization using PCR-amplified tomato p69a probe DNA, E. lathyris genome was suggested to have a gene family consisting of 3-5 gene members putatively encoding subtilisin-like proteases.