• Title/Summary/Keyword: 3D culture system

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Factors Affecting Callus Culture and Plant Regeneration in Kentucky Bluegrass (켄터키 블루그래스에 있어서 캘러스 배양 및 식물체 재분화에 미치는 요인의 영향)

  • Lee, K.W.;Lee, S.H.;Lee, D.G.;Woo, H.S.;Kim, D.H.;Choi, M.S.;Won, S.H.;Seo, S.;Lee, B.H.
    • Journal of Animal Science and Technology
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    • v.47 no.6
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    • pp.1067-1074
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    • 2005
  • In order to optimize tissue culture conditions of Kentucky bluegrass(Poa pratensis L.), effects of culture medium supplements, media and cultivars on embryogenic callus induction and regeneration of plants were investigated. MS medium containing 3mg/L 2,4-D and 0.1mg/L BA was optimal for embryogenic callus induction from mature seeds. The highest plant regeneration frequency(57.7%) was observed when the embryogenic calli were cultured on N6 medium supplemented with 1mg/L 2,4-D and 3mg/L BA. Among several basic media, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. Genotype was an important factor in plant regenerability. ‘Newport’ showed to have higher regeneration frequency of 53.4%. Regenerated plants were grown normally when shoots transplanted to the soil. A short tissue culture period and high-frequency regeneration system would be beneficial for molecular breeding of Kentucky bluegrass through genetic transformation.

A Low Cost 3D Skin Wrinkle Reconstruction System Based on Stereo Semi-Dense Matching (반 밀집 정합에 기반한 저가형 3차원 주름 데이터 복원)

  • Zhang, Qian;WhangBo, Taeg-Keun
    • Journal of Internet Computing and Services
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    • v.10 no.4
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    • pp.25-33
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    • 2009
  • In the paper, we proposed a new system to retrieve 3D wrinkle data based on stereo images. Usually, 3D reconstruction based on stereo images or video is very popular and it is the research focus, which has been applied for culture heritage, building and other scene. The target is object measurement, the scene depth calculation and 3D data obtained. There are several challenges in our research. First, it is hard to take the full information wrinkle images by cameras because of light influence, skin with non-rigid object and camera performance. We design a particular computer vision system to take winkle images with a long length camera lens. Second, it is difficult to get the dense stereo data because of the hard skin texture image segmentation and corner detection. We focus on semi-dense stereo matching algorithm for the wrinkle depth. Compared with the 3D scanner, our system is much cheaper and compared with the physical modeling based method, our system is more flexible with high performance.

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A Study on the Official Uniform in the Early Years of the Balhae Era (발해 건국 초기 관복(官服)에 대한 소고(小考))

  • Jeon, Hyun-Sil;Kang, Soon-Che
    • Journal of the Korean Society of Costume
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    • v.57 no.2 s.111
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    • pp.59-68
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    • 2007
  • It seems that Balhae(渤海) had built up its national power through the international relationship from the early years of an establishment of a dynasty. And the envoys that were sent with the official duties dressed up the official uniform for a diplomatic courtesy. Accordingly, this study will be considered about the official uniform in the early years of the Balhae era. The system of the official uniform of Balhae was classified into two form as the starting from $739\sim742$ A.D. The official uniform of the early years of the Balhae($698\sim739$ A.D.) was based on the costume of the three Kingdoms[三國時代], that is, Yu, Go(待), Jo-u-gwan(鳥羽冠). These results were available owing to the reasons that the Balhaehad succeeded the culture of Go-gu-ryo(高句麗), which was the basis for the development of Parhae's own culture, and the most of the governing group and the ruling class in Balhaewere the drifting people from Go-gu-ryo. After that, which the 3th King Mun(文王), acrepted the Tang's culture positively, assumed the reins of government ascended the throne and the brisk exchange with Tang(唐) by the envoys and Suk-wui(宿衛) were dispatched to Tang, the official uniform of the Balhaewas transformed into the system of Tang. This system was similar to that of Tang in point of using a four colors and the items as Danryeong(團領), Bokdu.

Optimization of Culture Conditions for D-Ribose Production by Transketolase-Deficient Bacillus subtilis JY1

  • Park, Yong-Cheol;Seo, Jin-Ho
    • Journal of Microbiology and Biotechnology
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    • v.14 no.4
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    • pp.665-672
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    • 2004
  • D-Ribose is a five-carbon sugar used for the commercial synthesis of riboflavin, antiviral agents, and flavor enhancers. Batch fermentations with transketolase-deficient B. subtilis JY1 were carried out to optimize the production of D-ribose from xylose. The best results for the fermentation were obtained with a temperature of $37^{\circ}C$ and an initial pH of 7.0. Among various sugars and sugar alcohols tested, glucose and sucrose were found to be the most effective for both cell growth and D-ribose production. The addition of 15 g/l xylose and 15 g/l glucose improved the fermentation performance, presumably due to the adequate supply of ATP in the xylose metabolism from D-xylulose to D-xylulose-5-phosphate. A batch culture in a 3.7-1 jar fermentor with 14.9 g/l xylose and 13.1 g/l glucose resulted in 10.1 g/l D-ribose concentration with a yield of 0.62 g D-ribose/g sugar consumed, and 0.25 g/l-h of productivity. Furthermore, the sugar utilization profile, indicating the simultaneous consumption of xylose and glucose, and respiratory parameters for the glucose and sucrose media suggested that the transketolase-deficient B. subtilis JY1 lost the glucose-specific enzyme II of the phosphoenolpyruvate transferase system.

Enhanced Enzyme Activities of Inclusion Bodies of Recombinant ${\beta}$-Galactosidase via the Addition of Inducer Analog after L-Arabinose Induction in the araBAD Promoter System of Escherichia coli

  • Jung, Kyung-Hwan
    • Journal of Microbiology and Biotechnology
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    • v.18 no.3
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    • pp.434-442
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    • 2008
  • We observed that an inclusion body (IB) of recombinant ${\beta}$-galactosidase that was produced by the araBAD promoter system in Escherichia coli (E. coil) showed enzyme activity. In order to improve its activity, the lowering of the transcription rate of the ${\beta}$-galactosidase structural gene was attempted through competition between an inducer (L-arabinose) and an inducer analog (D-fucose). In the deep-well microtiter plate culture and lab-scale fermentor culture, it was demonstrated that the addition of D-fucose caused an improvement in specific ${\beta}$-galactosidase production, although ${\beta}$-galactosidase was produced as an IB. In particular, the addition of D-fucose after induction led to an increase in the specific activity of ${\beta}$-galactosidase IB. Finally, we confirmed that the addition of D-fucose after induction caused changes in the structure of ${\beta}$-galactosidase IB, with higher enzyme activity. Based on these results, we expect that an improved enzyme IB will be used as a biocatalyst of the enzyme bioprocess, because an enzyme IB can be purified easily and has physical durability.

Study on torso patterns for elderly obese women for vitalization of the silver clothing industry - Applying the CLO 3D program - (실버 의류산업 활성화를 위한 노년 비만여성의 토르소 원형 연구 - CLO 3D 가상착의 시스템 활용 -)

  • Seong, Ok jin;Ha, Hee Jung
    • The Research Journal of the Costume Culture
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    • v.25 no.4
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    • pp.476-487
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    • 2017
  • The purpose of this study was to suggest torso patterns that fit the three main body shapes of elderly obese women. To reduce time, costs, and also the trial and error needed to make patterns, the CLO program for 3D test wear was employed. Three virtual models for aged obese women were use, with the YUKA system used to produce torso patterns. 3D simulation of test wear and corrections was done to design optimal torso patterns. The results were as follows: First, for the three models of obese women's body shapes as realized by CLO 3D, Type 1 is lower-body obesity shapes, Type 2 is abdominal obesity shapes, and Type 3 is whole-body obesity shapes. Second, to design the study patterns, actual measurement values, back waist length and waist to hip length, were used. The armhole depth (B/4-1.5), front interscye (B/6+2.3), front neck width (B/12-0.5), front neck depth (B/12+0.5), front waist measurement (W/4+ 1.5+D), front hip measurement (H/4+2+0.5), and back hip measurement (H/4+3-0.5) were calculated using formulas. Third, according to the results of test-wearing the study patterns, reduced front neck width and depth improved the neck fit and reduced armhole depth bettered loose or plunging armhole girth and also reduced the sagging of bust c.. Also, tight sidesfrom aprotruded waist and abdomen improved with the increase of surpluses in the back waist and also back and front hip c. The exterior was enhanced by displacement of back and front darts, which distributed surpluses better.

Building up the foundation for the elderly apparel industry through the development on shirt sloper of elderly obese males - Applying CLO 3D program - (노년 비만남성의 셔츠원형 개발을 통한 실버 의류산업 활성화 기반 구축 - CLO 3D 가상착의 시스템 활용 -)

  • Seong, Ok jin;Kim, Sook jin
    • The Research Journal of the Costume Culture
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    • v.28 no.3
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    • pp.299-312
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    • 2020
  • The purpose of this study is to create a shirt sloper suitable for an elderly male body shape by producing virtual models using a 3D-virtualization program, making a torso prototype using the Yuka CAD system, and employing 3D simulation to virtualize and calibrate the model. First, the following three types of obese dummies are implemented through the CLO 3D program: Type 1 exhibits body fat in the lower body; Type 2 exhibits an obese abdomen; and Type 3 displays a balanced form of obesity. Second, for the design of the shirt pattern, the waist back length (measured value+1), back armhole depth (C/10+12+3+0.5~1.5), front armhole depth (back armhole depth 0~1), front interscye (2C/10-1+0.5-0.5), armscye depth (C/10+2+3.5+ 0.5), back interscye (2C/10-1+1), front chest C (C/4+2.5+1), back chest C (C/4+2.5-1), front hem C (C/4+2.5+1(+2)), back hem C (C/4+2.5-1(+2)), cap height (AH/3-5), and biceps width (Front AH-1, Back AH-1) are calculated. Third, the virtual attachment of the shirt pattern is resolved by increasing the front and back armhole depths, and the front and rear wrinkles are improved by adding a back armhole dart. The front hem lift and lateral pull caused by the protrusion of the abdomen are amended by increasing the margin of the chest, waist C, and hip C, with the appearance improved by balanced margin distribution in the front, back, and side panels. The improved retail pattern with an increase in the front armholes C was balanced on the torso plate.

Comparisons of Ion Balance, Fruit Yield, Water, and Fertilizer Use Efficiencies in Open and Closed Soilless Culture of Paprika (Capsicum annuum L.)

  • Ko, Myat Thaint;Ahn, Tae In;Son, Jung Eek
    • Horticultural Science & Technology
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    • v.31 no.4
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    • pp.423-428
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    • 2013
  • Although closed soilless culture is useful for saving water and fertilizers with minimizing environmental pollution, adequate management of nutrient solutions is still not stabilized in greenhouse cultivation. In order to investigate the problems occurred in closed soilless culture of Paprika (Capsicum annuum L., cv. Fiesta), we compared ion balance, fruit yield, and the water and fertilizer use efficiencies in the closed system with those in the open system. The plants were grown in rockwool culture with a nutrient solution of EC $2.5dS{\cdot}m^{-1}$. After 4 weeks of treatment, individual ratio of $NO{_3}^-$, $K^+$, $Ca^{2+}$, and $Mg^{2+}$ to total ion concentrations ($meq{\cdot}L^{-1}$) decreased from the initial value, especially the biggest decrement was observed in $K^+$, and on the other hand, $SO{_4}^{2-}$, $Cl^-$, and $Na^+$ were accumulated in the closed system. Yields after four-time harvests were 19% higher in the open system than in the closed system. Total volume of water used per unit area ($m^2$) in the open system was 20% higher, but the total water use per fruit was not significantly different between the two systems, while t total fertilizer use per fruit was 78% higher in the closed system. Amount of marketable fruits was not significantly different between the two systems. We concluded that the increase in $K^+$ supply and the replenishment of recycled nutrient solution every four weeks were required for preventing the imbalance or depletion of nutrients in the close soilless culture of paprika plants to get more balanced nutrient composition during whole cultivation period.

An Investigative Stucy on the Farmland Uses in Korea and Hokkaido of Japan (韓國과 日本 北海道에서의 農地利用에 관한 調査硏究)

  • Kim, Ki-Sung;Yasuharu, Uneda;Choi, Ye-Hwan
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.33 no.3
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    • pp.81-90
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    • 1991
  • The purpose of the this study was to investigate and analyze the various statistical data on the actual state of farmland uses given in Korea and Hokkaido of Japan. The results of this research are as follows ; 1. The rate of farmland uses was marked 118% and 99% in Korea and Hokkaido of Japan in 1988, respectively. That of Korea was higher than that of Hokkaido of Japan. The tendency of the value for Korea has been decreased since the value was shown peak in 1980, but that of the value for Hokkaido was found gradually the increased value. It was analyzed that the reason why the value was decreased in Korea was referred to avoid the crops culture, and the reason why the value was increased in Hokkaido was dueto increase the area of pasture. 2. The farmland use system according to region were d ue to be the mixing pattern of rice culture and dry-field crops in East region of korea and the type of mainly rice culture in West region. The pattern of farmland use system was used to be performed mainly the rice culture in Central region, the mixing type of rice culture and dry-field crops in South region, and the agriculture of mainly dry-field crops and dairy in East-North region in Hokkaido. 3. In the light of the rate of planting, the rate of rice culture and dry-field crops were 49% and 51% in Korea, respectively. As the values were 13% and 87% in Hokkaido of Japan, respectively, the dry-field crops attained the superiority. The main crop kinds was shown the rice culture in Korea and the feed plant in Hokkaido of Japan, respectively. 4. In considering the above results, there are much differences in agricultural style and industrialization processes in both country Korea and Japan. Especially much more differences were evident from farmland use system of Hokkaido Island where had unique agricultural style in Japan.

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