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http://dx.doi.org/10.5187/JAST.2005.47.6.1067

Factors Affecting Callus Culture and Plant Regeneration in Kentucky Bluegrass  

Lee, K.W. (Division of Applied Life Science, Gyeongsang National University)
Lee, S.H. (Division of Applied Life Science. Gyeongsang National University)
Lee, D.G. (Division of Applied Life Science, Gyeongsang National University)
Woo, H.S. (Division of Applied Life Science, Gyeongsang National University)
Kim, D.H. (Division of Applied Life Science, Gyeongsang National University)
Choi, M.S. (Division of Forest Science, Gyeongsang National University)
Won, S.H. (Institute of Agri. Sci. & Tech., Kyungpook National University)
Seo, S. (National Livestock Research Institute, R.D.A.)
Lee, B.H. (Division of Applied Life Science, Gyeongsang National University)
Publication Information
Journal of Animal Science and Technology / v.47, no.6, 2005 , pp. 1067-1074 More about this Journal
Abstract
In order to optimize tissue culture conditions of Kentucky bluegrass(Poa pratensis L.), effects of culture medium supplements, media and cultivars on embryogenic callus induction and regeneration of plants were investigated. MS medium containing 3mg/L 2,4-D and 0.1mg/L BA was optimal for embryogenic callus induction from mature seeds. The highest plant regeneration frequency(57.7%) was observed when the embryogenic calli were cultured on N6 medium supplemented with 1mg/L 2,4-D and 3mg/L BA. Among several basic media, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. Genotype was an important factor in plant regenerability. ‘Newport’ showed to have higher regeneration frequency of 53.4%. Regenerated plants were grown normally when shoots transplanted to the soil. A short tissue culture period and high-frequency regeneration system would be beneficial for molecular breeding of Kentucky bluegrass through genetic transformation.
Keywords
Embryogenic callus; Forage crop; Plant tissue culture; Transformation;
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