• 무역상무연구
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• 제23권
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• pp.3-38
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• 2004

On-line game business has emerged as the most lucrative entertainment industry, with over 4 million players in China, with more than 45% of the market dominated by Korean on-line games. While the interactive entertainment market continues to expand, with many new Korean on-line game publishers entering the market, relatively little is known about which factors influence online game players' behavioral intentions (i.e., intention to play, brand-identity, word of mouth, flow, etc.) in this area. It appears that in this extremely attractive and growing services segment, academics, as well as practitioners, would benefit from investigating the relationships among the players' intention to play, social norms, word of mouth, flow, and the impacts of several other key game-related constructs. A conceptual framework is proposed and a simultaneous equation model is developed. This paper develops the exploratory LISREL model for identifying the factors affecting the players' intention to play for on-line games. Based on data collected from on-line questionnaire survey in China, the validity of the model has been tested and interesting conclusions have been developed concerning the relationships between the game-related constructs. It is hoped that this result might provide the useful guidelines for developing the successful on-line game contents. With better understanding of the players' behavioral intentions, Korean on-line game developers should be able to penetrate the China market with sustainable advantage over their competition.

• BMB Reports
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• 제46권12호
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• pp.594-599
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• 2013

The anti-inflammatory activity of eriodictyol and its mode of action were investigated. Eriodictyol suppressed tumor necrosis factor (mTNF)-${\alpha}$, inducible nitric oxide synthase (miNOS), interleukin (mIL)-6, macrophage inflammatory protein (mMIP)-1, and mMIP-2 cytokine release in LPS-stimulated macrophages. We found that the anti-inflammatory cascade of eriodictyol is mediated through the Toll-like Receptor (TLR)4/CD14, p38 mitogen-activated protein kinases (MAPK), extracellular-signal-regulated kinase (ERK), Jun-N terminal kinase (JNK), and cyclooxygenase (COX)-2 pathway. Fluorescence quenching and saturation-transfer difference (STD) NMR experiments showed that eriodictyol exhibits good binding affinity to JNK, $8.79{\times}10^5M^{-1}$. Based on a docking study, we propose a model of eriodictyol and JNK binding, in which eriodictyol forms 3 hydrogen bonds with the side chains of Lys55, Met111, and Asp169 in JNK, and in which the hydroxyl groups of the B ring play key roles in binding interactions with JNK. Therefore, eriodictyol may be a potent anti-inflammatory inhibitor of JNK.

• 대한화장품학회지
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• 제38권3호
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• pp.201-207
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• 2012

Oil-in-ethanol (O/E) 마이크로에멀젼을 물에 희석하여 얻은 O/W 나노에멀젼의 성질에 대하여 다른 희석 과정의 영향을 연구하였다. 물/에탄올/비이온성계면활성제/실리콘 오일 계를 모델 계로 선택하였다. 희석과정은 물(또는 마이크로에멀젼)을 마이크로에멀젼(또는 물)에 단계별로 첨가하는 방법으로 구성되었다. O/E 마이크로에멀젼을 물에 첨가하여 혼합하면 30 nm 정도의 입경을 가진 나노에멀젼을 얻을 수 있었다. 반면에 물을 O/E 마이크로에멀젼에 첨가하면 400 nm의 입경을 가진 에멀젼을 얻을 수 있다. 희석 방법이 얻어지는 에멀젼의 성질에 중요한 역할을 하였다. 시간에 따른 나노에멀젼의 입자 변화는 관찰되지 않았으나 입자가 큰 에멀젼은 시간에 따라 입경이 증가하였으며 불안정화 기작은 오스트왈드 라이퍼닝으로 추정되었다.

• Reproductive and Developmental Biology
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• 제29권2호
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• pp.101-108
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• 2005

Tumor necrosis factor-related apoptosis inducing ligand (TRAIL) is a well-known inducer of apoptotic cell death in many tumor cells. 1RAIL is expressed in human placenta, and cytotrophoblast cells express 1RAIL receptors. However, the role of TRAIL in human placentas and cytotrophoblast cells is not. well understood. In this study a trophoblast cell line, JEG-3, was used as a model system to examine the effect of TRAIL. on key intracellular signaling pathways involved in the control of trophoblastic cell apoptosis and survival JEG-3 cells expressed receptors for 1RAIL, death receptor (DR) 4, DR5, decoy receptor (OcR) 1 and DeR2. Recombinant human TRAIL (rhTRAIL) did not have a cytotoxic effect determined by MIT assay and did not induce apoptotic cell death determined by poly-(ADP-ribose) polymerase cleavage assay. rhTRAIL induced a rapid and transient nuclear translocation of nuclear $factor-{\kappa}B(NF-{\kappa}B)$ determined by immunoblotting using nuclear protein extracts. rhTRAIL rapidly activated extracellular signal-regulated protein kinase (ERK) 1/2 as determined by immnoblotting for phospho-ERK1/2. However, c-Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinase (p38MAPK) and Akt (protein kinase B) were not activated by rhTRAIL. The ability of 1RAIL to induce $NF-{\kappa}B$ and ERK1/2 suggests that interaction between TRAIL and its receptors may play an important role in trophoblast cell function during pregnancy.

• 한국통신학회논문지
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• 제38B권4호
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• pp.247-256
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• 2013

2011년 9월 IEEE 802.15.7 워킹그룹(Working group)을 통해 LED와 PD를 이용하는 가시광 무선 통신 기술에 대한 물리 계층과 MAC 계층에 대한 표준 규격이 완성됨에 따라 가시광 무선 통신 기술을 이용한 다양한 응용 서비스 기술 개발의 토대가 마련되었다. 이에 따라 가시광 무선 통신 기술은 상용화를 위한 응용 연구 단계로 진입하였으나 대부분의 가시광 무선 통신 환경 개발 현황이 PtP(Peer to peer) 환경을 고려하고 있다. 따라서 보다 다양한 응용 서비스의 개발을 위해서는 PtMP(Point to multi-point) 환경에 대한 연구가 요구된다. 이에 따라 본 논문에서는 IEEE 802.15.7 표준에서 정의한 물리계층과 MAC 계층의 특성을 반영한 스타 토폴로지 기반의 VPAN(Visible personal area network) 시스템 시뮬레이터를 구현하였고, 이를 기반으로 MAC 프로토콜에 영향을 끼치는 주요 파라미터들에 대한 네트워크 성능을 분석하였다.

• Journal of Biosystems Engineering
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• 제38권4호
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• pp.327-334
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• 2013

Purpose: The purpose of this study was to analyze for resonant frequency, vibration transmissibility and damping ratio of multi-layered corrugated structures using a random vibration test. Methods: The random vibration test was performed by the ASTM D4728 specifications using two paperboards (S120, K180) and two types of flutes (A/F, B/F). Damping ratio of the multi-layered corrugated structures was estimated using a theoretical equation derived from the measured resonant frequency and transmissibility. Results: The resonant frequency and vibration transmissibility of the multi-layered corrugated structures of K180 and B-flute were higher than those of S120 and A-flute, respectively; however, the damping ratio of each sample had the opposite tendency. The resonant frequency was inversely proportional to the sample thickness and static stress; vibration transmissibility and damping ratio were not correlated with sample thickness and static stress. In addition, we developed a mathematical model of the resonant frequency with variables of sample thickness and static stress. Conclusions: Results of this study can be useful for environment-friendly and optimal packaging design since vibration has been a key factor in cushioning packaging design.

• 산업경영시스템학회지
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• 제38권3호
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• pp.78-86
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• 2015

Up-to-date business environment for manufacturers is very complex and rapidly changing. In other words, companies are facing a variety of changes, such as diversifying customer requirements, shortening product life cycles, and switching to small quantity batch production. In this situation, the companies are introducing the concept of JIT (just-in-time) to solve the problem of on-time production and on-time delivery for survival. Though many companies have introduced ERP (enterprise resource planning) systems and MRP (material requirement planning) systems, the performance of these systems seems to fall short of expectations. In this paper, the case study on introducing an APS (advanced planning and scheduling) system based on dispatching rules to a machining company and on finding a method to establish an efficient production schedule is presented. The case company has trouble creating an effective production plan and schedule, even though it is equipped with an MRP-based ERP system. The APS system is applied to CNC (computer numerical control) machines, which are key machines of the case company. The overall progress of this research is as follows. First, we collect and analyze the master data on individual products and processes of the case company in order to build a production scheduling model. Second, we perform a pre-allocation simulation based on dispatching rules in order to calculate the priority of each order. Third, we perform a set of production simulations applying the priority value in order to evaluate production lead time and tardiness of pre-defined dispatching rules. Finally, we select the optimal dispatching rule suitable for work situation of the case company. As a result, an improved production schedule leads to an increase in production and reduced production lead time.

• Journal of Pharmaceutical Investigation
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• 제38권2호
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• pp.99-104
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• 2008

PLGA micro/nano particles encapsulating ranitidine as a hydrophilic model drug were prepared by the double-emulsion solvent evaporation method. Surface morphology investigation by scanning electron microscope (SEM) showed that the emulsification by sonication could produce nanoparticles, whereas microparticles were prepared using high speed homogenizer. Moreover, while nanohalf-shell structure instead of spherical nanoparticle could be produced by adding poloxamer into oil phase (MC) with PLGA 504H, the addition of poloxamer didn't change particle shape in case of PLGA 502H. On the other hand, microparticle with poloxamer had more surface pores than those without poloxamer. The size and polydispersity (PDI) of particles were determined by particle size analyzer. Effective diameters of particles were in the range of $400{\sim}800\;nm$ and $1200{\sim}3300\;nm$ in case of nanoparticles and microparticles, respectively. Encapsulation efficiencies were in the range of $1.2{\sim}2.9%$. The addition of poloxamer produced the particles with higher encapsulation efficiency. In vitro release study in phosphate buffer (pH 7.4) at $37^{\circ}C$ showed common large initial burst release. However, the relative slower release profile could be observed in case of microparticles. Poloxamer addition increased the release rate, which was thought to be related to the increased surface area of particles.

• 대한기계학회논문집B
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• 제38권12호
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• pp.1027-1034
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• 2014

회전원판 위 표면에 작용하는 유동 전단응력은 표면마모를 발생시키는 주원인이다. 유체는 원심력에 의해 가장자리까지 자유표면을 갖는 박막유동으로 전달된다. 표면마모는 받음각 또는 곡률에 따른 전단응력 정도에 비례하여 차이를 보이게 된다. 전산해석을 통한 속도분포 기울기로 받음각에 기준한 곡률의 전단응력비를 비교하였다. 곡률반경 변화에 따른 전단유동의 응력감소를 모델링하여 표면마모를 효율적으로 줄일 수 있는 최적구간을 결정하는 것이 본 연구의 주제이며, 이 연구결과는 회전무화나 박막코팅과 같은 박막유동을 활용하는 기구의 최적설계에 적용될 수 있다.

• IMMUNE NETWORK
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• 제21권3호
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• pp.23.1-23.16
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• 2021

Chemokines are key factors that influence the migration and maintenance of relevant immune cells into an infected tissue or a tumor microenvironment. Therefore, it is believed that the controlled administration of chemokines in the tumor microenvironment may be an effective immunotherapy against cancer. Previous studies have shown that CCL3, also known as macrophage inflammatory protein 1-alpha, facilitates the recruitment of dendritic cells (DCs) for the presentation of tumor Ags and promotes T cell activation. Here, we investigated the role of CCL3 in regulating the tumor microenvironment using a syngeneic mouse tumor model. We observed that MC38 tumors overexpressing CCL3 (CCL3-OE) showed rapid regression compared with the wild type MC38 tumors. Additionally, these CCL3-OE tumors showed an increase in the proliferative and functional tumor-infiltrating T cells. Furthermore, PD-1 immune checkpoint blockade accelerated tumor regression in the CCL3-OE tumor microenvironment. Next, we generated a modified CCL3 protein for pre-clinical use by fusing recombinant CCL3 (rCCL3) with a non-cytolytic hybrid Fc (HyFc). Administering a controlled dose of rCCL3-HyFc via subcutaneous injections near tumors was effective in tumor regression and improved survival along with activated myeloid cells and augmented T cell responses. Furthermore, combination therapy of rCCL3-HyFc with PD-1 blockade exhibited prominent effect to tumor regression. Collectively, our findings demonstrate that appropriate concentrations of CCL3 in the tumor microenvironment would be an effective adjuvant to promote anti-tumor immune responses, and suggest that administering a long-lasting form of CCL3 in combination with PD-1 blockers can have clinical applications in cancer immunotherapy.