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A Functional MRI Study on the Brain Activation Associated with Mental Calculation (기능적 자기공명영상을 이용한 수리연산의 대뇌 활성화에 관한 연구)

  • Choi Dae Seob;Na Dong Gyu;Kim Sam Soo;Cho Jae Min;Park Eui Dong;Chung Sung Hoon;Ryoo Jae Wook
    • Investigative Magnetic Resonance Imaging
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    • v.9 no.1
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    • pp.16-23
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    • 2005
  • Purpose : In order to investigate the functional brain anatomy associated with mental calculation, functional magnetic resonance imaging was performed. Materials and Methods : In six normal right handed subjects, functional MR images were obtained using a 1.57 MR scanner and the EPI BOLD technique. The study included experiment I and experiment II. Each experiment consisted of five resting and four activation periods with each period of 30 seconds. During the activation period of both experiment I and II, calculation equations[an example: $(4+5)\times8=72$] were presented and the subjects were instructed to decide true or false of them. During the resting period of experiment I, the subjects were instructed to visually fixate on a crosshair. During the resting period of experiment II, two diagrams (an example: $(\bullet,\;\blacksquare)$)were presented and the subjects were instructed to decide they are same or not. For the post-processing of images, the SPM program was used, with the threshold of significance set at p<0.00001. The activated areas during the tasks were assessed. Results : In experiment 1, the inferior frontal gyrus, prefrontal cortex, promoter area, supplementary motor area, and intraparietal sulcus including superior parietal cortex were activated bilaterally. Although these areas were also activated in experiment II, the activated signals in the right frontal and parietal lobes were lessened. Conclusion : The left inferior frontal gyrus and prefrontal cortex and bilateral intraparietal sulci were activated during mental calculation. The right frontal and parietal lobes might be related to attention and decision making.

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Flora, Actual Vegetation Map, and Primary Production of the Vascular Hydrophytes and Hygrophytes in the Upo Wetland (우포늪에서 수생 및 습생 관속식물의 식물상, 현존식생도 및 1차 생산)

  • Kang, Min-jeong;Kim, Cheol-Soo;Oh, Kyung-hwan
    • Journal of Wetlands Research
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    • v.9 no.2
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    • pp.45-55
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    • 2007
  • Flora, actual vegetation map, distribution area by the life form, primary productivity and annual primary production by the vascular hydrophytes and hygrophytes were investigated in the Upo wetland, Changnyeong-gun, Gyeongsangnam-do, Korea from May 2005 to March 2006. The flora of Upo, Mokpo, Sajipo, Jokjibyeol, Topyeongcheon upstream, and Topyeongcheon downstream were composed of 263, 233, 244, 182, 190, and 178 taxa, respectively. The flora of total study area was 85 families, 224 genera, 287 species, 42 varieties, 4 form, or total 333 taxa. Among them, hydrophytes, hygrophytes, and others were 38, 108, and 187 taxa, respectively. The life form of the vascular hydrophytes was classified as 20 taxa of emergent plants, 6 taxa of floating-leaved plants, 5 taxa of free-floating plants, and 7 taxa of submersed plants, respectively. There were 27 plant communities including pure population, mixed population, and etc. It is also found that Trapa japonica-Ceratophyllum demersum community occupies 60.64 ha, the largest area, and Salvinia natans-Ceratophyllum demersum community 32.91 ha, Zizania latifolia community 30.05 ha, and that the area of free-floating plants was the largest as 172.6 ha(47.9%) on the basis of life form. Total annual primary production of the vascular hydrophytes and hygrophytes was 1,383.3ton. That of the emergent hydrophytes was the most as 564.1 ton(40.8%), and those of the free-floating, floating-leaved, and the submersed were 484.1 ton(34.9%), 146.7 ton(10.6%), and 1.3 ton(0.5%), respectively, and the hygrophytes was 182.1 ton(13.2%). Since most plant species are fairly adapted to the present marsh environment, bad influences and change of species composition are expected by the artificial influences on the wetland such as fragmentation, reclamation, and introduction of the exotic species. Therefore, schemes and counterplans for the conservation and preservation of the marsh are demanded.

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Lithium Extraction from Smectitic Clay Occurring in Lithium-bearing Boron Deposits in Turkey (터키 리튬 함유 붕소광상에서 산출하는 스멕타이틱-점토로부터의 리튬 추출)

  • Lee, Won-Jong;Yoon, Soh-joung;Chon, Chul-Min;Heo, Chul-Ho;Lee, Gill-Jae;Lee, Bum-Han;Cicek, Murat
    • Journal of the Mineralogical Society of Korea
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    • v.29 no.4
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    • pp.167-177
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    • 2016
  • Smectitic clays, occurring in Kırka and Bigadiç boron evaporite deposits formed in Miocene playa lake environment in Turkey, contain $LiO_2$ 0.02-0.21% and 0.16-0.30%, respectively, and boron tailings are also reported to contain $LiO_2$ 0.04-0.26%. Lithium in smectitic clays was identified to be retained in hectorite. The XRD results revealed that hectorite was contained in 25.7% and 79.7% of Kırka and Bigadiç deposit samples respectively. In this study, we selected a clay sample from each deposit with lithium content of ~0.18% and estimated extractable lithium by acid treatment and roasting method commercially applicable to lithium resources, such as lepidolite and hectorite. When 1 g of crushed clay (particle size less than $74{\mu}m$) was reacted with 200 mL of 0.25 M HCl solution, the amount of lithium dissolved increased with the increase of reaction time up to 10 hours for both samples. Reaction time longer than 10 hours did not significantly increased the amount of lithium dissolved. After 10 hours of reaction, 89% of lithium in the clay sample from the Kırka deposit was dissolved, while 71% of lithium was dissolved from the Bigadiç deposit tailing sample. 87% of lithium in the clay sample from the Kırka deposit was extracted and 82% of lithium was extracted from the Bigadiç deposit tailing sample by the roasting extraction method, where clays were leached after a thermal treatment at $1,100^{\circ}C$ for 2 hours with $CaCO_3$ and $CaSO_4$.

Synthesis and Structural Characterization of Benzene-sorbed Cd2+-Y(FAU) Zeolite (벤젠이 흡착된 Cd2+-Y(FAU) 제올라이트의 합성 및 구조연구)

  • Moon, Dae Jun;Suh, Jeong-Min;Park, Jong Sam;Choi, Sik Young;Lim, Woo Taik
    • Journal of the Mineralogical Society of Korea
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    • v.30 no.2
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    • pp.45-57
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    • 2017
  • Two single crystals of fully dehydrated $Cd^{2+}$-exchanged zeolites Y were prepared by the exchange of ${\mid}Na_{75}{\mid}[Si_{117}Al_{75}O_{384}]-FAU$ ($Na_{75}-Y$, Si/Al = 1.56) with aqueous $0.05M\;Cd(NO_3)_2$ (pH = 3.65) at 294 K, followed by vacuum dehydration at 723 K (crystal 1) and a second crystal, similarly prepared, was exposed to zeolitically dried benzene for 72 hours at 294 K and evacuated (crystal 2). Their structures were determined crystallographically using synchrotron X-rays and were refined to the final error indices using $F_o$>$4{\sigma}(F_o)$ of $R_1/wR_2=0.040/0.121$ and 0.052/0.168, respectively. In crystal $1({\mid}Cd_{36}H_3{\mid}[Si_{117}Al_{75}O_{384}]-FAU)$, $Cd^{2+}$ ions primarily occupy sites I and II, with additional $Cd^{2+}$ ions at sites I', II', and a second site II. In crystal $2({\mid}Cd_{35}(C_6H_6)_{24}H_5{\mid}[Si_{117}Al_{75}O_{384}]-FAU)$, $Cd^{2+}$ ions occupy five crystallographic sites. The 24 benzene molecules are found at two distinct positions within the supercages. The 17 benzene molecules are found on the 3-fold axes in the supercages where each interacts facially with one of site IIa $Cd^{2+}$ ions. The remaining 7 benzene molecules lie on the planes of the 12-rings where each is stabilized by multiple weak electrostatic and van der Waals interactions with framework oxygens.

Analysis of Functional Constituents of Mulberries (Morus alba L.) Cultivated in a Greenhouse and Open Field during Maturation (시설 및 노지 재배 오디의 숙기에 따른 기능성 성분 함량 분석)

  • Kim, Ha Yun;Lee, Ji Young;Hwang, In Guk;Han, Hye Min;Park, Bo Ram;Han, Gui Jung;Park, Jong Tae
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.10
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    • pp.1588-1593
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    • 2015
  • Contents of phenolic acids, flavonoids, and anthocyanins of mulberries (Morus alba L.) cultivated in a greenhouse (GH) and open field (OF) were evaluated by HPLC during maturation. In the case of phenolic acids, caffeic acid ($96.37{\sim}824.00{\mu}g/g$), coumaric acid ($19.45{\sim}68.23{\mu}g/g$), ferulic acid ($4.50{\sim}18.66{\mu}g/g$), and sinapic acid ($15.61{\sim}29.27{\mu}g/g$) were detected. The major phenolic acid was caffeic acid, and its content increased in the initial stage and decreased in the last stage. The phenolic acid content of OF mulberries was higher than that of GH mulberries. Contents of two major flavonoids, quercetin, and kaempferol, were $44.17{\sim}1,661.73{\mu}g/g$ and $108.67{\sim}360.20{\mu}g/g$, respectively. Quercetin content decreased with maturation stage, whereas kaempferol content remained constant in GH mulberries but increased in OF mulberries. In the case of cultivation methods, quercetin content of OF mulberries was higher than that of GH mulberries. Contents of cyanidin, delphinidin, malvidin, and pelargonidin were $30.43{\sim}6,443.88{\mu}g/g$, $0{\sim}52.10{\mu}g/g$, $1.06{\sim}1,650{\mu}g/g$, and $1.92{\sim}401.97{\mu}g/g$, respectively. Anthocyanin content increased with maturation stage, and anthocyanin content of OF mulberries was higher than that of GH mulberries. OF mulberries in the last stage of maturation had higher contents of functional substances than other conditions.

A Study on Results of Rehabilitation Therapy of Patients with Head Injury (두부손상 환자의 재활치료 결과에 관한 연구)

  • Kim, Su-Min;Lim, Hye-Hyeon
    • Journal of Korean Physical Therapy Science
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    • v.1 no.1
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    • pp.135-144
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    • 1994
  • The authors studied therapeutic effects and related clinical data retrospectively on a series of 48 consecutive patients with head injury who were referred to physical therapy and occupational therapy, Kosin University Medical Center during 1 year, from March, 1993. through March, 1994. The conclusions were as follows : 1. Average age of the subject patients was 50.6 years, their age remped from 14 to 72 years, and the incidence between male and femele was 1:1.1. 2. In respect to the educational level high school graduates showed the highest incidence 18 (39.1 %) of 46 cases, and in respect to economy level, middle class revealed the highest incidence, 31(64.6 %) in 48 cases. 3. The HBP was the most common cause of spontaneity injury, disclosing 19 cases in 35 patients(54.3%), whereas the main cause of traumatic injury was a traffic accident, 7 cases ( 53.8 %) in 13 patients. 4. As for the region of injury in the cases of spontaneity ICH was 14 persons (40%). In the cases of trauma, hematoma was seen in 6 cases(42.2 %) in 48 total cases ICH was seen in 17 cases (35.4 %) to be the most common region. 5. Among 35 persons, spontaneity CBR was the highest region of injury, 13 cases(37.1 %) ; the side of paralysis in extremities the right side showed higher incidence, 18 cas (1.4 %) as compared to the left, 16 cases(45.7%). In the case of traumatic injury, CBR was the highest region of injury, 4(30.8% )in 13 cases and as for the paralysis side, right side showed higher incidence 7 cases(53.8%) as compared to the left side 5 cases(38.5%). 6. In respect to recurrence, HBP was seen in 5 cases to be the most common cause, and as for the region of injury, CBR was the highest. 7. A period of rehabilitation therapy in the cases of physical therapy the highest term was 1-2 month, 14 persons(29.2%). As for occupational therapy within 2 week-term was the highest, 24 persons(50%). 8. Physical strenth grade M.T in the cases of the upper extrimities before therapy, the low mark(grade 0) was 30 cases(62.5%), compared to the high mark(above grade 3)seen in 1 case(2.1%), And after therapy, the lowest mark(grade 0)was seen in 5 cases(10.4%) where as the high mark(above grade 3)was seen in 29 cases(60.4%). In the case of the lower extremities before therapy, the mark(below grade 1)was 37 cases(77.1 %), while the high mark(above grade 3)was seen n 4 cases(8.4%). And after therapy the low mark(below grade 1)was seen in 5 cases (10.4 %) and the high mark (above grade 3)was seen in 29 cases(60.4 %). 9. Before therapy conigtive function-level evaluation utilized R.L.A.L, the low mark(below level 3)was seen in 9 cases(18.8%), while the high mark(above level 7)was seen in 18 cases(37.5%). And after therapy the low mark (below level 3)was 4 cases(8.3%) and high mark(above level 7)was seen in 38 cases(79%). 10. After rehabilitation therapy, patients who were able to walk independently were 29 persons(60.4%), among which 16 cases(55.2%) depended on cane. The ratio between the cases who were able to walk and unable to walk was 1.5:1.

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Hatching Rate of Eggs, and Growth of Larvae and Juveniles from Selected Olive Flounder, Paralichthys olivaceus (선발 육종된 넙치, Paralichthys olivaceus의 부화율 및 자치어 성장)

  • Min, Byung-Hwa;Lee, Jeong-Ho;Noh, Jae-Koo;Kim, Hyun-Chul;Park, Choul-Ji;Choi, Sang-Jun;Myeong, Jeong-In
    • Development and Reproduction
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    • v.13 no.4
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    • pp.239-247
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    • 2009
  • Hatching rate, larval deformation and growth rate of selected olive flounder (Paralichthys olivaceus) for rapid growth were compared to those of the unselected fish. Fish were spawned on the same day and cultured under the similar conditions. The selected fish had a significantly higher eggs hatching rate, and lower larval deformation. The selected fish grew significantly faster, and at the end of the experiment (8 weeks after hatching) averaged 50.49${\pm}$2.67 mm in total length, 16.30${\pm}$0.08 mm in body height, and 1.036${\pm}$0.118 g in weight, compared to 40.55${\pm}$3.13 mm, 13.50${\pm}$0.96 mm, and 0.557${\pm}$0.073 g for unselected fish, respectively. The selected fish had a significantly higher body shape index, however lower condition factor than the unselected fish. The results of the present study demonstrate that the selected fish of the olive flounder for rapid growth had superior growth and improved body shape.

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Analysis of dutasteride in human serum by LC-MS/MS (LC-MS/MS를 이용한 혈청 중 dutasteride 분석)

  • Nam, Hye-Seon;Nam, Kyong-Hee;Jung, Su-Hee;Lee, Jang-Woo;Kang, Jin-Yeong;Hong, Soon-Keun;Kim, Tae-Sung;Jung, Ki-Kyung;Kang, Tae-Seok;Yoon, Hae-Jung;Lee, Kwang-Ho;Rhee, Gyu-Seek
    • Analytical Science and Technology
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    • v.25 no.1
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    • pp.76-82
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    • 2012
  • The determination and confirmation of dutasteride in human serum was performed by a liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI/MS/MS). Beclomethasone as an internal standard (I.S.) was added to the serum and the mixed sample was pretreated by liquid-liquid extraction (LLE) with methyl tert-butyl ether (MTBE). The mass transitions of dutasteride and I.S. monitored in multiple reaction monitoring (MRM) were m/z 529.6${\rightarrow}$461.5 and m/z 409.3${\rightarrow}$391.2, respectively, and the retention times were 6.45 and 5.46 min, respectively. The calibration curve was linear in the concentration range of 0.5~30.0 ng/mL ($R^2$= 0.9999) and the limit of quantitation (LOQ) was found to be 0.5 ng/mL. The recovery of dutasteride was shown to be 66~72%. The intra-day assay precision and accuracy were in the range 3.5~5.5% and 85.7~89.9%, respectively, and the interday assay precision and accuracy were in the range 4.2~5.8% and 90.8~95.8%, respectively.

Short-term Follow up Study of Calcific Tendinitis Using Ultrasonography Guided Injection (석회화 건염에 대한 외래 초음파 유도 주사 치료요법의 단기추시결과)

  • Sim, Jung-Hyun;Kwon, Jae-Bum;Park, Chang-Min;Choi, Chang-Hyuk
    • The Journal of Korean Orthopaedic Ultrasound Society
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    • v.4 no.2
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    • pp.77-83
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    • 2011
  • Purpose: We compared with USG-guided steroid injection group and blind steroid injection group for the treatment of calcific tendinitis to evaluate the effectiveness of the treatment modalities. Materials and Methods: We reviewed two groups of calcific tendinitis treated with steroid injection and follow up upto 6 month after injection. Group I was blind injection for 88 patient with average age of 53years old and female gender in 77%. Group II was USG- guided injection for 102 patients with average age of 54years old and female gender in 85%. We compared follow up results according to age, gender, size of tendon involvement, initial VAS score, and phase of the calcific tendinitis between two groups. Results: At 6 month's follow up period, USG-guided injected group was more prevalent compared to blind injection group with 92%(94/102) and 72%(64/88) (P<0.05). In Group I, 77%(49/64) patients have improved symptom. And in Group II, 92%(86/94) patients have improved symptom in formative or resorptive phase. Despite of symptom improvement, calcium deposit is remained 47%(30/64) in Group I, and 20%(19/94) in Group II. Conclusion: Conservative treatment of Shoulder calcific tendinitis patients through USG-guided injection is more effective than Blind injection in pain relief & calcium decrease. The patients USG-guided injected group was more prevalent in follow up at outpatient clinic, throughout more accurate injection with improved confidence.

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Na, K-ATPase Activity in the Aged Erythrocytes of Hypertensive Rats (고혈압쥐 노화 적혈구에서의 Na, K-ATPase에 관한 연구)

  • Park, Chang-Kil;Hur, Gang-Min;Seok, Jung-Ho;Lee, Jae-Heun
    • The Korean Journal of Pharmacology
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    • v.27 no.1
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    • pp.33-43
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    • 1991
  • To study the age dependent change of Na, K-ATPase in the erythrocyte of hypertensive rat, 1-kidey 1-clip hypertensive rat was made by the removal of right kidney and partial ligation of left renal artery. After 4 weeks, aged erythrocyte fraction was separated by density gradient centrifugation, and Na, K-ATPase activity and $^3H-ouabain$ binding with ghost cell membrane and ouabain sensitive Rb-uptake with whole cell were measured. 1) In the hypertensive rats, blood pressure was significantly increased to 165.5/119.0 mmHg (systolic/diastolic). Mean corpuscular volume and membrane protein(mg) per $10^9RBC$ were decreased and hemoglobin content was increased in the aged erythrocyte. 2) Na, K-ATPase activity in the solution containing 110 mM NaCl and 10 mM KCI, was decreased in hypertensive rat, and decreased in aged erythrocyte of both group. 3) Ouabain sensitive Rb-uptake by low RbCl concentration(4 mM) was slightly decreased in aged erythrocyte compared to that in young erythrocyte of each group, but slightly increased in young erythrocyte in hypertensive rat compared to that in normotensive rat. 4) Ouabain sensitive Rb-uptake by high RbCl concentration(16 mM) was decreased about 30% to 50 % in aged erythrocyte in both group. And in hypertensive rat, especially in young erythrocyte it was significantly decreased compared to that in normotensive rats. 5) $^3H-ouabain$ binding at 0.13 or $1{\times}10^-6M$ ouabain concentration was slightly decreased in aged erythrocyte of normotensive rat, and significantly decreased in aged erythrocyte of hypertensive rats. 6) $^3H-ouabain$ binding at 6 or $64{\times}10^-6M$ ouabain concentration is slightly decreased in aged erythrocyte of both group, but significantly decreased in young and aged erythrocyte of hypertensive rats compared to that of normotensive rats. The present results suggest that (1) in the young erythrocyte of hypertensive rat, the alterations of Na-pump activity that slightly increased in weak stimulation and inhibited in strong stimulation, may be related to increased molecular activity and the decrease in the number of low affinity site without change in high affinity site, (2) in the aged erythrocyte of normotensive rat, inhibited Na-pump may be related to the change in molecular activity of pump. (3) And in the aged erythrocyte of hypertensive rat, it may be related to the decrease in the number of high and low affinity site as well as the change in molecular activity

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