• 인지과학
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• 제14권4호
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• pp.33-42
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• 2003

본 논문은 내적상태의 차원모형을 기반으로 한 얼굴 표정인식을 위한 새로운 시스템을 제시한다. 얼굴표정 정보는 3단계로 추출된다. 1단계에서는 Gabor 웨이브렛 표상이 얼굴 요소들의 경계선을 추출한다. 2단계에서는 중립얼굴상에서 얼굴표정의 성긴 특징들이 FCM 군집화 알고리즘을 사용하여 추출된다. 3단계에서는 표정영상에서 동적인 모델을 사용하여 성긴 특징들이 추출된다. 마지막으로 다층 퍼셉트론을 사용하여 내적상태의 차원모델에 기반한 얼굴표정 인식을 보인다. 정서의 이차원 구조는 기본 정서와 관련된 얼굴표정의 인식 뿐만 아니라 다양한 정서의 표정들로 인식할 수 있음을 제시한다.

• 한국컴퓨터산업학회논문지
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• 제8권3호
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• pp.187-196
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• 2007

사람들은 의식적이든 무의식적이든 표정을 통해 감정을 표현하며 살아간다. 이러한 표정을 인식하려는 시도가 몇 몇 심리학자에 의해 시작되어 과거 10여년동안 컴퓨터 과학자들에게도 관심분야가 되었다. 표정인식은 인간과 컴퓨터의 인터페이스를 기반으로 하는 여러 분야에 응용할 수있는 미래가치가 높은 분야이다. 그러나 많은 연구에도 불구하고 조명변화, 해상도, 고차원의 정보 처리 등의 어려움으로 실용화된 시스템을 찾아보기 힘들다. 본 논문에서는 표정 분석을 위한 기본 프레임워크를 기술하고 각 단계의 필요성과 국외의 연구동향을 기술하였으며 국내의 표정에 관한 연구사례를 분석하였다. 이를 통해 국내에서 표정분석에 기여하고자 하는 연구자들에게 도움이 되기를 기대한다.

• Korean Chemical Engineering Research
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• 제56권6호
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• pp.826-831
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• 2018

본 연구는 다중 형광이 표지된 이중 분획 입자의 제조에 관한 것이다. 입자 내에서 형광 발현을 분획화하기 위하여, 형광의 여기 및 방출 스펙트럼의 중첩이 적은 두가지의 형광 염료를 선정한다. 또한, 형광 안정성을 확보하기 위하여 선정된 형광 염료는 입자를 구성하는 소재와 함께 가교될 수 있도록 분자 내에 아크릴레이트(acrylate) 작용기를 포함한다. 공초점 현미경 촬영을 통하여 선정된 형광 물질을 이용하여 제조된 입자에서 강한 형광 발현 및 형광의 분획화를 확인하였다. 더 나아가 4주 동안 형광 발현 및 세기를 측정하여 장기간의 형광 안정성을 검증하였다. 본 연구에서 제조된 다중 형광 표지된 이중 분획 입자는 다중 표적형 약물 전달 체계, 3차원 브라운 운동의 해석 연구, 3차원의 복잡한 자기 조립체 형상의 규명 연구 등에 널리 활용될 수 있으리라 기대한다.

• 대한공간정보학회지
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• 제18권4호
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• pp.141-149
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• 2010

3차원 공간정보는 효율적 국토이용 및 관리, 지자체의 도시계획수립, 도시관리 등 도시 활동의 입체적인 표현과 분석을 위한 중요한 정보로써 공공분야뿐만 아니라 공간정보 서비스 산업 활성화로 민간분야에서도 다양하게 이용되고 있다. 고품질 3차원 공간정보의 생성을 위해서는 원시영상 및 3차원 지형모델의 품질 뿐만 아니라 LoD 수준, Texturing과 같은 가시화 수준이 중요한 요소가 된다. 하지만 기존 3차원 국토공간정보는 구축 공정이 복잡하고, 기 제작된 수치지도를 이용하여 자료의 최신성이 부족하다. 또한 일반정사영상의 이용으로 영상의 기복변위가 존재하여 가시성이 낮고, LoD 수준이 2～3급 정도로 인공지물의 3차원 모델이 단순화 되어 현실감이 다소 부족하다는 단점이 있다. 이에 본 논문에서는 기존의 대축척 디지털항공사진카메라와 다방향 촬영 디지털카메라로 촬영된 디지털항공사진영상을 이용하여 수치사진측량기법을 적용한 3차원 모델링 기법으로 제작된 3차원 공간정보의 품질 분석을 수행하였다. 3차원 모델의 가시화 정보의 정확도 분석결과 별도의 가시화 정보의 획득 없이 원영상만으로 84% 이상의 정확도를 확보할 수 있었다. 촬영시기와 동일한 3차원 공간정보 구축이 가능하여 자료의 최신성 확보가 용이 하였고, 작업공정의 실감정사영상의 위치정확도 분석결과 1:1,000 수치지도의 수평위치 허용정확도보다 양호한 결과를 나타냈다.

• 패션비즈니스
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• 제17권1호
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• pp.30-41
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• 2013

Augmented reality techniques have been increasingly employed in the textile and fashion industry as well as computer graphics sectors. Three-dimensional virtual clothing CAD systems have also been widely used in the textile industries and academic institutes. Motion tracking techniques are grafted together in the 3D and augmented reality techniques in order to develop the virtual three-dimensional clothing and fitting systems in the fashion and textile industry sectors. In this study, three-dimensional virtual clothing sample has been prepared using a 3D virtual clothing CAD along with a 3D scanning and reconstruction system. Motion of the user has been captured through an RGB-D sensor system, and the virtual clothing fitted on the user's body is allowed to move along with the captured motion flow of the user. Acutal fabric specimens are selected for the material characterization. This study is a primary step toward building a comprehensive system for the user to experience interactively virtual clothing under real environment.

• 패션비즈니스
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• 제24권5호
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• pp.106-124
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• 2020

The purpose of this study was to analyze the three-dimensional (3D) characteristics and reproducibility of the effective expression of North American smocking pleats in the process of making clothes using a 3D virtual clothing system (CLO) and present a method of expression according to the types of North American smocking. In this study, lattice, lozenge, and flower smocking were produced as real smocking and 3D virtual content, and actual muslin properties were measured using a Fabric Kit and reflected using an emulator. The results of this study confirmed that a dense puckered design such as North American smocking could be expressed depending upon the internal line, fold angle, and reinforcement setting for 3D smocking. To partially apply pleats to flat fabrics, it was necessary to set fold lines. The fold line setting could be expressed by designing the internal line in horizontal, vertical, and diagonal directions according to the North American smocking design, and then setting the fold angle for each internal line. By setting fold angles of 0 degrees and 360 degrees according to the folding direction of the set internal line, the fabric was clearly folded and stable pleats were created. This study will contribute to the vitalization of the 3D virtual fashion content industry by analyzing and presenting the optimal expression method of sophisticated and complex pleats generated according to the North American smocking design pattern.

• 방사선산업학회지
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• 제5권1호
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• pp.1-5
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• 2011

Since proteomics can be employed to compare changes in the expression levels of many proteins under particular genetic and environmental conditions, using mass spectrometry to establish radiation stimulon, we performed two-dimensional gel electrophoresis and identified E. coli proteins whose expressions are affected by high dose of ionizing radiation. After exposure to 3 kGy, it was found that 6 proteins involved in carbon and energy metabolism were reduced. Although 4 of 7 protein spots showing a significant increase in expression level were neither identified nor classified, uridine phosphorylase (Udp), superoxide dismutase (SodB), and thioredoxin-dependent thiol peroxidase (Bcp) were proven to be up-regulated after irradiation. This suggests that E. coli subjected to high doses of radiation (3 kGy) may operate a defense system that is able to detoxify reactive oxygen species and stimulate the salvage pathway of nucleotide synthesis to replenish damaged DNA.

• 한국수정란이식학회지
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• 제29권3호
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• pp.207-219
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• 2014

Three-dimensional (3D) culture system is useful technique for study of in vivo environment and it was used various experiments. This study was investigated to establish of embryo co-culture system and changes of PAs activity in 3D cultured endometrial cells of pigs. In results, growth of stromal cells into gel matrix were detected only with endometrial and myometrial cells. The most rapid growth of stromal cells were confirmed in $2.5{\times}10^5cells/ml$ and gel matrix containing 15% FBS. Expression of urokinase-PA (uPA) after treatment of hCG (0.5, 1.0, 1.5 and 2.0 IU/ml) were higher than without hCG, but, there are not significant difference among the treatment. On the other hand, expression of uPA after treatment of $IL-1{\beta}$ (0.1, 1, 10 and 100 ng/ml) were higher than without $IL-1{\beta}$, but, there are not significant difference. Expression of uPA after treatment of estrogen (0.2, 2, 20 and 200 ng/ml) were not difference, but PA activity was significantly decreased (p<0.05). Blastocyst was producing in PZM-3 medium containing FBS and endometrial cells were grown in PZM-3 medium. When embryos development with cultured endometrial cells, cleavage rates were not significant difference and blastocyst were not produced in co-culture with stromal cells and 3D culture system. 3D culture system had similar activity to in vivo tissue and these features are very useful for study of in vivo physiology. Nevertheless 3D culture system was not proper in embryo co-culture system. Therefore, we suggest that 3D culture system with embryo co-culture need continuous research.

• BMB Reports
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• 제56권7호
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• pp.398-403
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• 2023

Natural killer (NK) cells are an essential part of the innate immune system that helps control infections and tumors. Recent studies have shown that Vorinostat, a histone deacetylase (HDAC) inhibitor, can cause significant changes in gene expression and signaling pathways in NK cells. Since gene expression in eukaryotic cells is closely linked to the complex three-dimensional (3D) chromatin architecture, an integrative analysis of the transcriptome, histone profiling, chromatin accessibility, and 3D genome organization is needed to gain a more comprehensive understanding of how Vorinostat impacts transcription regulation of NK cells from a chromatin-based perspective. The results demonstrate that Vorinostat treatment reprograms the enhancer landscapes of the human NK-92 NK cell line while overall 3D genome organization remains largely stable. Moreover, we identified that the Vorinostat-induced RUNX3 acetylation is linked to the increased enhancer activity, leading to elevated expression of immune response-related genes via long-range enhancer-promoter chromatin interactions. In summary, these findings have important implications in the development of new therapies for cancer and immune-related diseases by shedding light on the mechanisms underlying Vorinostat's impact on transcriptional regulation in NK cells within the context of 3D enhancer network.

• Annals of dermatology
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• 제30권6호
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• pp.694-700
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• 2018

Background: Kaempferol (3,4',5,7-tetrahydroxyflavone) is a flavonoid known to have a wide range of pharmacological activities. The 3-OH group in flavonoids has been reported to determine antioxidant activities. Objective: We tested whether kaempferol can affect the expression of integrins and the stem cell fate of interfollicular epidermal stem cells. Methods: Skin equivalent (SE) models were constructed, and the expression levels of stem cell markers and basement membrane-related antigens were tested. The immunohistochemical staining patterns of integrins, p63, and proliferating cell nuclear antigen (PCNA) were compared between kaempferol- and apigenin-treated SE models. Reverse transcription-polymerase chain reaction (RT-PCR) was used to evaluate the mRNA expression of integrins. Results: Kaempferol increased the thickness of the epidermis when added to prepare SEs. In addition, the basal cells of kaempferol-treated SEs appeared more columnar. In the immunohistological study, the expression of integrins ${\alpha}6$ and ${\beta}1$ and the numbers of p63- and PCNA-positive cells were markedly higher in the kaempferol-treated model. However, apigenin showed no effects on the formation of three-dimensional skin models. RT-PCR analysis also confirmed that kaempferol increased the expression of integrin ${\alpha}6$ and integrin ${\beta}1$. Conclusion: Our findings indicated that kaempferol can increase the proliferative potential of basal epidermal cells by modulating the basement membrane. In other words, kaempferol can affect the fate of interfollicular epidermal stem cells by increasing the expression of both integrins ${\alpha}6$ and ${\beta}1$. These effects, in particular, might be ascribed to the 3-OH group of kaempferol.