• 제목/요약/키워드: 3-D culture

검색결과 2,087건 처리시간 0.03초

광도측정을 통한 3D 복원 (Photometric Stereo from single view image)

  • 방정원
    • 한국컴퓨터정보학회:학술대회논문집
    • /
    • 한국컴퓨터정보학회 2016년도 제54차 하계학술대회논문집 24권2호
    • /
    • pp.285-286
    • /
    • 2016
  • 본 논문에서는 3D 콘테츠와 이를 가공하기 위한 기술로, 한 물체에 여러 방향으로 빛이 주어진 사진들을 이용하여 해당 물체의 3D shape를 재현해 낼 수 있는 Photometric Stereo에 대해 구현 및 테스트해 보고, 1개의 카메라를 사용하여 3D 콘텐츠를 구현해 낼수 있는 방안들에 대하여 살펴 본다.

  • PDF

From Visualization to Computer Animation Approaches in Mathematics Learning: the Legacy throughout History of Human Endeavours for Better Understanding

  • Rahim, Medhat H.
    • 한국수학교육학회지시리즈D:수학교육연구
    • /
    • 제17권4호
    • /
    • pp.279-290
    • /
    • 2013
  • Presently, there has been growing interests in using mathematics' history in teaching mathematics [Katz, V. & Tzanakis, C. (Eds.) (2011). Recent Developments on Introducing a Historical Dimension in Mathematics Education. Washington, DC: Mathematical Association of America]. Thus, this article introduces some work of scholars from ancient East Indian culture like Bhaskara (AD 1114-1185) and Arabic culture such as Ibn Qurrah (AD 9th c) that are related to Pythagoras Theorem. In addition, some Babylonian creative works related to Pythagorean triples found in a tablet known as 'Plimpton 322', and an application of the Pythagorean Theorem found in another tablet named 'Yale Tablet' are presented. Applications of computer animation of dissection Motion Operations concept in 2D and 3D using dynamic software like Geometer's-Sketchpad and Cabri-II-and-3D. Nowadays, creative minds are attracted by the recent stampede in the advances of technological applications in visual literacy; consequently, innovative environments that would help young students, gifted or not, acquiring meaningful conceptual understanding would immerge.

Development of a Three-dimensional Hydrogel System for the Maintenance of Porcine Spermatogonial Stem Cell Self-renewal

  • Park, Ji Eun;Park, Min Hee;Kim, Min Seong;Yun, Jung Im;Choi, Jung Hoon;Lee, Eunsong;Lee, Seung Tae
    • 한국수정란이식학회지
    • /
    • 제32권4호
    • /
    • pp.343-351
    • /
    • 2017
  • Porcine spermatogonial stem cells (SSCs) prefer three-dimensional (3D) culture systems to 2D ones for the maintenance of self-renewal. Of the many 3D culture systems, agar-based hydrogels are candidates for supporting porcine SSC self-renewal, and there are various types of agar powder that can be used. In this study, we sought to identify an agar-based 3D hydrogel system that exhibited strong efficacy in the maintenance of porcine SSC self-renewal. First, 3D hydrogels with different mechanics were prepared with various concentrations of Bacto agar, lysogeny broth (LB) agar, and agarose powder, and the 3D hydrogel with the strongest alkaline phosphatase (AP) activity and greatest increase in colony size was identified for the different types of agar powder. Second, among the porcine SSCs cultured in the different 3D hydrogels, we analyzed the colony formation, morphology, and size; AP activity; and transcription and translation of porcine SSC-related genes, and these were compared to determine the optimal 3D hydrogel system for the maintenance of porcine SSC self-renewal. We found that 0.6% (w/v) Bacto agar-, 1% (w/v) LB agar-, and 0.2% (w/v) agarose-based 3D hydrogels showed the strongest maintenance of AP activity and the most pronounced increase in colony size in the culture of porcine SSCs. Moreover, among these hydrogels, the strongest transcription and translation of porcine SSC-related genes and largest colony size were detected in porcine SSCs cultured in the 0.2% (w/v) agarose-based 3D hydrogel, whereas there were no significant differences in colony formation and morphology. These results demonstrate that the 0.2% (w/v) agarose-based 3D hydrogel can be effectively used for the maintenance of porcine SSC self-renewal.

고추 약배양을 통한 고빈도 배발생 및 식물체 재분화 (High Frequency Embryogenesis and Plant Regeneration in Anther Cultures of Pepper)

  • 안민영;김용권;민성란;정원중;인동수;유장렬
    • 식물조직배양학회지
    • /
    • 제25권5호
    • /
    • pp.319-322
    • /
    • 1998
  • 여러 계통의 고추(Capsicm annuum L.)의 약을 이용하여 식물체 재분화 조건을 확립하였다. 꽃받침 위로 꽃잎이 2mm 올라온 미성숙 화뢰에서 상부로부터 절반 이하로 착색된 약을 채취하여 0.1mg/L 2,4-D와 0.1mg/L kinetin이 첨가된 Dumas 고체배지에 치상하였다. 배양 4주후부터 배가 발생하였으며, 8주후의 배발생 빈도는 최고 58.3%였다. 배는 MS 기본배지로 옮겨주었을 때 95이상이 발아하여 식물체로 재분화되었다.

  • PDF

Neuroprotective Effect of Acanthopanax sessiliflorus against Toxicity Induced by N-Methyl-D-Aspartate in Rat Organotypic Hippocampal Slice Culture

  • Lee, Pyeong-Jae;Lee, Sang-Hyun;Choi, Sang-Yoon;Son, Dong-Wook
    • Natural Product Sciences
    • /
    • 제11권3호
    • /
    • pp.179-182
    • /
    • 2005
  • We investigated that water extract of Acanthopanax sessiliflorus roots rescued the N-methyl-D-aspartate (NMDA), agonist of glutamate receptor, -induced toxicity in rat organotypic hippocampal slice culture. When the cell death in NMDA only-treated hippocampal slices was set 100%, A. sessiliflorus decreased the cell death to 75.4, 51.6, 48.9, and 40.6% at 1, 10, 50, and $100\;{\mu}g/ml$ treatment, respectively. On the basis of these results, the water extract of A. sessiliflorus roots may be a preventive agent against NMDA-induced cytotoxicity.

폴리스티렌 나노섬유상에서의 간세포의 3차원 배양 (3-D Rat Hepatocytes' Culture on Polystyrene Nanofibrous Scaffold)

  • 김영진;안창현;오환희;김영진;윤관한;강인규;권오형
    • 폴리머
    • /
    • 제32권2호
    • /
    • pp.131-137
    • /
    • 2008
  • 전기방사법에 의해 폴리스테렌 나노섬유 부직포를 제조하였고, 간세포의 접착을 유도하기 위해 간세포접착 리간드인 Poly[N-p-vinylbenzyl-O-$\beta$-D-galactopyranocyl-($1{\rightarrow}4$)-D-gluconamide](PVLA)를 나노섬유의 표면에 코팅하였다. 얻어진 나노섬유는 평균직경이 500 nm이었고 분광학적인 방법을 통해 PS 나노섬유 표면의 개질 여부를 확인하였다. 장기간 배양시의 간세포 생존율을 MTT법으로 조사한 결과 기존의 세포배양접시에서는 급격하게 세포생존율이 감소함에 비해 PVLA가 코팅된 나노섬유상에서는 생존율이 서서히 감소하였으며 약 3주간에 걸쳐 간세포가 생존율이 유지되었다. 간세포의 대표적인 기능인 알부민분비 및 요소분해능을 측정한 결과 기존의 세포배양접시에서는 급격하게 간세포기능이 저하되었지만, PVLA가 코팅된 나노섬유상에서는 약 3주정도 초기의 간세포기능을 유지하는 것이 밝혀졌다.

A Study on the Effective Production of Game Weapons Using ZBrush

  • YunChao Yang;Xinyi Shan;Jeanhun Chung
    • International Journal of Advanced Culture Technology
    • /
    • 제11권2호
    • /
    • pp.397-402
    • /
    • 2023
  • With the rapid adoption of 5G, the gaming industry has undergone significant innovation, with the quality of game content and player experience becoming the focal point of attention. ZBrush, as a professional digital sculpting software, plays a crucial role in the production of 3D game models. In this paper, we explore the application methods and techniques of ZBrush in game weapons production through specific case analyses. We provide a detailed analysis of two game weapon models, discussing the design and modeling process, lowto-high poly conversion, UV unwrapping and texture baking, material texture creation and optimization, and final rendering. By comparing the production process and analyzing the advantages and disadvantages of ZBrush, we establish a theoretical foundation for further design research and provide reference materials for game industry professionals, aiming to achieve higher quality and efficiency in 3D game model production.

맥각균(麥角菌)의 배양(培養)에 관한 연구(硏究)(IV) -한국산(韓國産) 맥각균(麥角菌)의 배양(培養)- (Studies on the Culture of Ergot Fungus(IV) -Culture of Korean Ergot Fungus-)

  • 김병각;심미자;최응칠;박영인
    • 생약학회지
    • /
    • 제6권1호
    • /
    • pp.9-13
    • /
    • 1975
  • To isolate ergot strains which are capable of growing and producing alkaloids in submerged culture, strains were isolated from the sclerotia parasitizing the Graminae plants in Korea and the experiments of submerged culture of these strains yielded the following results: 1) The mycelia which were respectively isolated from the sclerotia parasitizing Agropyron semicostatum $N_{EES}$, Arundinella hirta $T_{ANAKA}$ var. ciliata $K_{OIDSUMI}$, Ischaemum anthephoroides $M_{IQ}$. var. eriostachyum $H_{ONDA}$, and Phleum pretense L. grew in Medium D by submerged culture. 2) When the strain of the ergot of Agropyron semicostatum was inoculated into six different nutrient solutions for submerged culture, its mycelium grew well in Media C, D and F, but produced alkaloids only in Medium C, indicating that Medium C is relatively suitable for the strain. 3) The extraction of the alkaloids from the culture broth by ether and T.L.C. analysis of the extract showed that it contained at least two types of alkaloids.

  • PDF

Study on the Seed Production of Olive Flounder Paralichthys olivaceus in a Pilot Scale Recirculating Aquaculture System

  • Park, Jeong-Hwan;Jo, Jae-Yoon;Kim, Pyong-Kih
    • 한국양식학회지
    • /
    • 제21권3호
    • /
    • pp.125-132
    • /
    • 2008
  • Seed production of olive flounder Paralichthys olivaceus was performed in a pilot RAS. The growth of juvenile olive flounder and changes in water quality were monitored for the entire production period. The pilot RAS consisted of 8 circular culture tanks($4.0mD{\times}1.0mH$), 2 trickling biofilters($1.7mD{\times}2.0mH$), 2 protein skimmers ($0.8mD{\times}2.5mH$), and 4 sedimentation chambers($0.7mD{\times}1.5mH$). The culture surface area was about $100.5m^2$ and the actual working volume was about $106.9m^3$. As many as 300,000 fertilized olive flounder eggs were initially distributed into 2 culture tanks with the water temperature at $19.0^{\circ}C$. Live feeds such as rotifers and Artemia nauplii were fed until the 32nd day after hatching, and a commercial diet was fed from the 19th day to the end of the experiment. After 70 days, 150,256 juveniles with a body length of $65.8{\pm}3.9mm$ were produced in the RAS, with a daily growth rate for body length of 4.7%/day. At this time, the final culture density was 1,495 individuals $m^{-2}$, and 13.6 L of makeup water, 0.071 kW of electricity and 0.025 L of diesel fuel were used to produce a juvenile olive flounder. During metamorphosis of the larvae, the TAN concentration increased to 0.99 mg/L, which made the larvae sensitive to result in some mortality. However no more massive mortality occurred at the juvenile stage after metamorphosis even at a TAN concentration of 4.25 mg/L and a ${NO_2}^{-}-N$ concentration of 2.45 mg/L.