• Journal of Microbiology and Biotechnology
• /
• 제19권5호
• /
• pp.474-481
• /
• 2009

A novel microorganism, designated as LG12, was isolated from soil based on its ability to use acrylic acid as the sole carbon source. An electron microscopic analysis of its morphological characteristics and phylogenetic classification by 16S rRNA homology showed that the LG12 strain belongs to Rhodococcus erythropolis. R. erythropolis LG12 was able to metabolize a high concentration of acrylic acid (up to 40 g/l). In addition, R. erythropolis LG12 exhibited the highest acrylic acid-degrading activity among the tested microorganisms, including R. rhodochrous, R. equi, R. rubber, Candida rugosa, and Bacillus cereus. The effect of the culture conditions of R. erythropo/is LG12 on the production of 3-hydroxypropionic acid (3HP) from acrylic acid was also examined. To enhance the production of 3HP, acrylic acid-assimilating activity was induced by adding 1 mM acrylic acid to the culture medium when the cell density reached an $OD_{600}$ of 5. Further cultivation of R. erythropo/is LG 12 with 40 g/l of acrylic acid resulted in the production of 17.5 g/l of 3HP with a molar conversion yield of 44% and productivity of 0.22 g/l/h at $30^{\circ}C$ after 72 h.

• 한국융합학회논문지
• /
• 제8권7호
• /
• pp.167-173
• /
• 2017

본 연구는 조리기구와 친환경 식문화콘텐츠의 접목형 융합상품으로 최근 소비자의 니즈를 반영한 단순한 전시용도가 아닌 새로운 상품으로서의 가능성을 제시하였다. 또한, 상품화를 위하여 본 과제에서 제작할 디자인이 반영되도록 3D Rendering, 3D Mock-up 등을 활용해 별도의 제작공정을 들여 프로토타입 형태로 제작하고, 이후 생산자와 협의 등을 통해 다양한 상품으로 확장 고려 될 수 있을 것으로 판단된다. 국내는 물론 국외에도 적용할 수 있는 조리 기구를 개발, 디자인함으로써 각자가 자신의 취향에 맞게 식재료를 가열하거나 고기 등을 구워 먹을 수 있도록 한 제품으로 기존에 출시된 그릴 팬에서는 찾을 수 없는 보다 앞서가는 기능과 현대의 변화된 식문화에 잘 부합하며, 감성적이며 형태적으로 교류할 수 있는 디자인을 더하여 참신하고 혁신적인 주방제품 트렌드를 리드할 수 있도록 하였다.

• Tissue Engineering and Regenerative Medicine
• /
• 제15권6호
• /
• pp.781-791
• /
• 2018

BACKGROUND: Glucosamine hydrochloride (GlcN HCl) has been shown to inhibit cell growth and matrix synthesis, but not with N-acetyl-glucosamine (GlcNAc) supplementation. This effect might be related to an inhibition of critical growth factors (GF), or to a different metabolization of the two glucosamine derivatives. The aim of the present study was to evaluate the synergy between GlcN HCl, GlcNAc, and GF on proliferation and cartilage matrix synthesis. METHOD: Bovine chondrocytes were cultivated in monolayers for 48 h and in three-dimensional (3D) chitosan scaffolds for 30 days in perfusion bioreactors. Serum-free (SF) medium was supplemented with either growth factors (GF) $TGF-{\beta}$ ($5ng\;mL^{-1}$) and IGF-I ($10ng\;mL^{-1}$), GlcN HCl or GlcNAc at 1mM each or both. Six groups were compared according to medium supplementation: (a) SF control; (b) SF + GlcN HCl; (c) SF + GlcNAc; (d) SF + GF; (e) SF + GF + GlcN HCl; and (f) SF + GF + GlcNAc. Cell proliferation, proteoglycan, collagen I (COL1), and collagen II (COL2) synthesis were evaluated. RESULTS: The two glucosamines showed opposite effects in monolayer culture: GlcN HCl significantly reduced proliferation and GlcNAc significantly augmented cellular metabolism. In the 30 days 3D culture, the GlcN HCl added to GF stimulated cell proliferation more than when compared to GF only, but the proteoglycan synthesis was smaller than GF. However, GlcNAc added to GF improved the cell proliferation and proteoglycan synthesis more than when compared to GF and GF/GlcN HCl. The synthesis of COL1 and COL2 was observed in all groups containing GF. CONCLUSION: GlcN HCl and GlcNAc increased cell growth and stimulated COL2 synthesis in long-time 3D culture. However, only GlcNAc added to GF improved proteoglycan synthesis.

• Journal of Microbiology and Biotechnology
• /
• 제32권4호
• /
• pp.531-540
• /
• 2022

Due to the high incidence of malignant melanoma, the establishment of in vitro models that recapitulate the tumor microenvironment is of great biological and clinical importance for tumor treatment and drug research. In this study, 3D printing technology was used to prepare GelMA/PEGDA composite scaffolds that mimic the microenvironment of human malignant melanoma cell (A375) growth and construct in vitro melanoma micro-models. The GelMA/PEGDA hybrid scaffold was tested by the mechanical property, cell live/dead assay, cell proliferation assay, cytoskeleton staining and drug loading assay. The growth of tumor cells in two- and three-dimensional culture systems and the anti-cancer effect of luteolin were evaluated using the live/dead staining method and the Cell Counting Kit-8 (CCK-8) method. The results showed a high aggregation of tumor cells on the 3D scaffold, which was suitable for long-term culture. Cytoskeleton staining and immunofluorescent protein staining were used to evaluate the degree of differentiation of tumor cells under 2D and 3D culture systems. The results indicated that 3D bioprinted scaffolds were more suitable for tumor cell expansion and differentiation, and the tumor cells were more aggressive. In addition, luteolin was time- and dose-dependent on tumor cells, and tumor cells in the 3D culture system were more resistant to the drug.

• 디지털산업정보학회논문지
• /
• 제5권3호
• /
• pp.147-154
• /
• 2009

There are not much digital contents researches that do to culture relics basically. Digital contents research is studied by various method such as Cyber Gallery, 3-D Graphics, Hologram from Scanning that is the simplest form. This research wishes to do with internal culture relics as the central figure that it is that 'Sosu Seowon'. Korean culture contents cyber gallery construction can see as childhood yet. Present result that investigator develops research and as presentation about research process and contents result, wish to argue about problem. Digital contents escapes in existent physical time factor and development to new culture code is possible. Go and come often carrying out and search of fast data or experience of cultural inheritance is available. Through research, wished to present possible metheod actually in digital contents manufacture of internal cultural inheritance. Through this, expect role that offer base in contents manufacture skill.

• 대한공간정보학회지
• /
• 제17권4호
• /
• pp.45-51
• /
• 2009

세계 여러 나라들은 전통문화를 바탕으로 국익증진과 경쟁력 향상을 위해 문화콘텐츠의 개발 및 홍보방식에 새로운 변화와 개혁을 추진하고 있다. 또한, 국민의 생활수준과 삶의 질이 높아짐에 따라 전통 문화의 향유를 위한 다양한 요구가 증가하고 있으며, 문화재 환경이 갈수록 복잡하고 다양해지면서 체계적인 관리와 보존 방안이 필요하다. 이러한 관점에서 웹을 이용한 지형공간정보 제공서비스는 위성사진이나 지형, 3D 뷰잉 등 다양한 형태의 서비스를 제공함으로써 사용자가 특정지역의 구체적 공간정보를 얻을 수 있도록 하고 있다. 특히, 3D 모델링을 이용한 지형공간정보는 구축비용이나 운용, 유지의 어려움 때문에 국한된 공간에서만 이루어지고 있다. 본 논문에서는 전통 건축물 및 문화관광 자원을 3D 객체 단순화를 통해 구글어스에 효과적으로 업로딩하기 위한 방법을 제시하고자 한다.

• 복식문화연구
• /
• 제23권2호
• /
• pp.294-309
• /
• 2015

This study aims to clarify the aesthetic values between emotion of human and expression of technology in contemporary fashion as it analyzes formative characteristics of related cases in fashion based on principles of 3D Printing technology and the viewpoint of mechanic aesthetics. The conclusions of this study are as follows. First, 3D Printing fashion is not only expressed diverse variations by its principles of formative methods, materials and properties, but also changes of silhouette by applying system of designers. Second, general characteristics of 3D Printing fashion is represented by various applications in SLS system, and it can be specifically explained application to a portion of clothing, decorative roles of clothing, complicated pattern making through crossing fabrics using 3D scanner and displaying a certain object changing fashion styles, and so forth. Third, the formative characteristics of 3D Printing fashion from the perspective of mechanic aesthetics is as follows. It can be analyzed as the integration of metaphysical values through compared symbolization of natural feature and technical evolution, partial dynamics and interactive velocity-based, formative combinations for abstract expression using architectural components, cosmos images and substantialized structures through images of organic space interacted human shapes. As the mention above, 3D Printing technology can creative a diverse area of fashion, and express images of new technological fashion through various works with continuous development of techniques.

• 대한화장품학회:학술대회논문집
• /
• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book II
• /
• pp.174-183
• /
• 2003

To obtain effective and safe topical depigmenting agents, we synthesized hydroxybenzoates, alkoxybenzoates, and 3,4,5-trimethoxycinnamate containing a thymol moiety and screened then for high-level inhibitory activity against melanin synthesis. Among them, 5-methyl-2-(methylethyl)phenyl (2Ε)-3-(3,4,5-trimethoxyphenyl)prop-2-enoate (Melasolv)$^{TM}$ 4h, showed the most potent depigmenting effect ($IC_{50}$/ = 10$\mu$M) with low cytotoxicity ($IC_{50}$/ = 200$\mu$M). To find the inhibition mechanism of our candidate, various in vitro tests were performed such as DPPH assay, tyrosinase activity in mushroom or in culture cell and expression of tyrosinase, TRP-l and TRP-2. The result of this study suggested that 4h inhibited melanin synthesis by reducing the expression of tyrosinase and TRP-l at the transcriptional level in melan-a melanocytes.s.

• Animal cells and systems
• /
• 제5권3호
• /
• pp.205-209
• /
• 2001

Mesenchymal cells from the leg buds of stage 24-chick embryos differentiated into chondrocytes when plated at high density. Treatment of high density micromass culture of chick mesenchymal cells with cytochalasin D(CD, 2 $\mu$M for 24 h) resulted in inhibition of chondrogenesis. CD treatment was found to affect the expression of the contractile protein $\alpha$-smooth muscle actin ($\alpha$-SM actin). In control cultures, $\alpha$-SM actin uniformly expressed from culture day 2, but the CD-treated cells induced expression of $\alpha$-SM actin from the first day of culture followed by a continuous increase. Expression of pan-cadherin (P-cadherin) decreased as chondrogenesis proceeded in the control culture, whereas the CD-treated cells showed sustained expression. These results propose a close connection of chondrogenic differentiation with expression of $\alpha$-SM actin and P-cadherin.

• 식물조직배양학회지
• /
• 제25권5호
• /
• pp.335-339
• /
• 1998

미숙배주조직 유래의 배발생캘러스를 이용하여 원형질체의 유리 및 배양에 미치는 요인을 조사하였다. 배발생캘러스로부터 원형질체를 유리시키는데 적당한 배양시간은 16시간이었고, 건전한 원형질체를 유리하는데 적당한 효소용액의 농도는 0.7M $\textrm{BH}_{3}$ 용액과 cellulase 1.0%, macerozyme 1.0%, pctolyase 0.2%가 혼합된 효소용액을 동량으로 조합하였을 때가 가장 효과적이었다. 유리된 원형질체는 MT기본배지에 0.1 mg/L 2,4-D를 첨가한 배지로 배양하면 캘러스 형성이 양호하였다. 유도된 캘러스는 고체배지에 계대배양하고 있으나 생육이 징약한 상태이다. 본 실험결과 배발생캘러스유래의 원형질체와 엽육세포유래의 원형질체 융합에 의한 배양도 가능할 것으로 생각되었다.