Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Inhibition of Chondrogenesis by Cytochalasin D in High Density Micromass Culture of Chick Mesenchymal Cells: Its Effects on Expression of $\alpha$-Smooth Muscle Actin and P-cadherin

  • Yoo, Jeong-Ah (Department of Biology, College of Natural Sciences, Kyungpook National University) ;
  • Park, Su-Jung (Department of Biology, College of Natural Sciences, Kyungpook National University) ;
  • Kang, Shin-Sung (Department of Biology, College of Natural Sciences, Kyungpook National University) ;
  • Park, Tae-Kyu (Department of Biology, College of Natural Sciences, Kyungpook National University)
  • Published : 2001.09.01
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Abstract

Mesenchymal cells from the leg buds of stage 24-chick embryos differentiated into chondrocytes when plated at high density. Treatment of high density micromass culture of chick mesenchymal cells with cytochalasin D(CD, 2 $\mu$M for 24 h) resulted in inhibition of chondrogenesis. CD treatment was found to affect the expression of the contractile protein $\alpha$-smooth muscle actin ($\alpha$-SM actin). In control cultures, $\alpha$-SM actin uniformly expressed from culture day 2, but the CD-treated cells induced expression of $\alpha$-SM actin from the first day of culture followed by a continuous increase. Expression of pan-cadherin (P-cadherin) decreased as chondrogenesis proceeded in the control culture, whereas the CD-treated cells showed sustained expression. These results propose a close connection of chondrogenic differentiation with expression of $\alpha$-SM actin and P-cadherin.

Keywords

References

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