• Journal of Microbiology and Biotechnology
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• v.11 no.6
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• pp.915-921
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• 2001

Phytase improves the bioavailability of phytate phosphorus in plant foods to humans and animals, and reduces the phosphorus pollution of animal waste. In order to express a high level of fungal phytase in Saccharomyces cerevisiae, various expression vectors were constructed with different combinations of promoters, translation enhancers, signal peptides, and terminator. Three different promoters fused to the phytase gene (phyA) from Aspergillus niger were tested: a galactokinase (GAL1) promoter, glyceraldehyde-3-phosphate dehydrogenase (GPD) promoter, and yeast hybrid ADH2-GPD promoter consisting of alcohol dehydrogenase II (ADH2) and a GPD promoter. The signal peptides of phytase, glucose oxidase (GO), and rice amylase 1A(RAmy1A) were included. Plus, the translation enhancers of the ${\Omega}$ sequence and UTR70 from the tobacco mosaic virus (TMV) and spinach, respectively, were also tested. Among the recombinant vectors, pGphyA06 containing the GPD promoter, the ${\Omega}$ sequence, RAmy1A, and GAL7 terminator expressed the highest phytase activity in a culture filtrate, which was estimated at 20 IU/ml. An intracellular localization of the expressed phytase activity in a culture filtrate, which was estimated at 20 IU/ml. An intracellular localization of the expressed phytase was also performed by inserting an endoplasmic reticulum (ER) retention signal, KDEL sequence, into the C-terminus of the phytase within the vector pHphyA-6. It appeared that the KDEL sequence directed most of the early expression of phytase into the intracellular compartment yet more than $60\%$ of the total phytase activity was still retained within the cell even after the prolonged (>3 days) incubation of the transformant. However, the intracellular enzyme activity of the transformant without a KDEL sequence was as high as that of the extracellular one, thereby strongly suggesting that the secretion of phytase in S. cerevisiae appeared to be the rate-limiting step for the expression of a large amount of extracellular recombinant phytase, when compared with other yeasts.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.30 no.12A
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• pp.1153-1160
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• 2005

OFDM/OQAM(Offset QAM)-IOTA system requires the IOTA(Isotropic Orthogonal Transform Algorithm) function that has superior localization property in time and frequency domain instead of guard interval used for conventional OFDM/QAM system to be robust to multipath channel. Therefore, OFDM/OQAM-IOTA system has more spectral efficiency than conventional OFDM/QAM system. But, when channel estimation scheme for conventional OFDM/QAM system is applied straightforwardly to OFDM/OQAM-IOTA system, an intrinsic Inter- symbol-Interference is observed. So suitable preamble structure for the channel estimation scheme of OFDM/OQAM-IOTA system is required. In this paper, we propose a new preamble structure that is appropriate to OPDM/OQAM-IOTA system and then perform ideal channel estimation and practical channel estimation in low-to-medium mobile speed and compare them with conventional OFDM/QAM system. Simulation results show that OFDM/OQAM-IOTA system with proposed preamble structure has 1.5 dB Eb/NO gain on Target BER $10^{-3}$ and about $25\%$ transmission rate gain against the conventional OFDM/QAM system considering quarter of FFT size as guard interval size.

• Journal of Broadcast Engineering
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• v.2 no.1
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• pp.65-73
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• 1997

This paper propose a new triangular mesh approximation method using wavelet coefficients for large terrain data. Using spatio-freguency localization characteristics of wavelet coefficients, we determine the complexity of terrain data and approximate the data according to the complexity. This proposed algorithm is simple and requires low computational cost due to its top-down approach. Because of the similarity between the mesh approximation and data compression procedures based on wavelet transform, we combine the mesh approximation scheme with the Embedded Zerotree Wavelet (EZW) coding scheme for the effective management of large terrain data. Computer simulation results demonstrate that the proposed algorithm is very prospective for the 3-D visualization of terrain data.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.18 no.8
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• pp.98-103
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• 2019

A more accurate method of feature point extraction and matching for three-dimensional reconstruction using low-resolution images of crops is proposed herein. This method is important in basic computer vision. In addition to three-dimensional reconstruction from exact matching, map-making and camera location information such as simultaneous localization and mapping can be calculated. The results of this study suggest applicable methods for low-resolution images that produce accurate results. This is expected to contribute to a system that measures crop growth condition.

• Investigative Magnetic Resonance Imaging
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• v.25 no.3
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• pp.156-163
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• 2021

Recently, neuromelanin and nigrosome imaging techniques have been developed to evaluate the substantia nigra in Parkinson's disease. Previous studies have shown potential benefits of quantitative analysis of neuromelanin and nigrosome images in the substantia nigra, although visual assessments have been performed to evaluate structures in most studies. In this study, we investigate the potential of using deep learning based automatic region segmentation techniques for quantitative analysis of the substantia nigra. The deep convolutional neural network was trained to automatically segment substantia nigra regions on 3D nigrosome and neuromelanin sensitive MR images obtained from 30 subjects. With a 5-fold cross-validation, the mean calculated dice similarity coefficient between manual and deep learning was 0.70 ± 0.11. Although calculated dice similarity coefficients were relatively low due to empirically drawn margins, selected slices were overlapped for more than two slices of all subjects. Our results demonstrate that deep convolutional neural network-based method could provide reliable localization of substantia nigra regions on neuromelanin and nigrosome sensitive MR images.

• Progress in Superconductivity
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• v.3 no.1
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• pp.56-59
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• 2001

We have constructed a multi-channel SQUID magnetometer system for localization and classification of magnetic targets. Ten SQUID magnetometers were arranged to measure 5 independent components of 3 $\times$ 3 magnetic field gradient tensor. To get gradient from the difference of magnetic field measurements, we carefully balanced magnetometers. SQUIDs with slotted washer were used for operation in an unshielded laboratory environment, and noise characteristic in the laboratory was measured. With the multi-channel SQUID magnetometer system, we have successfully traced the motion of a bar magnet moving around it at a distance of about 1 m. In the urban environment, the drift of uniform magnetic field due to the irregular motion of a large magnetic body at distance and earth field causes an error in the position calculation, and this results in the distortion of the calculated trajectory. In this paper, we present the architecture and the performance of the system.

• Transactions on Control, Automation and Systems Engineering
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• v.3 no.4
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• pp.223-228
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• 2001

We have been developed an intelligent mobile robot with an artificial olfactory function to recognize odours and to track odour source location. This mobile robot also has ben installed an engine for speech recognition and synthesis and is controlled by wireless communication. An artificial olfactory system based on array of 7 gas sensors has been installed in the mobile robot for odour recognition, and 11 gas sensors also are located in the obttom of robot to track odour sources. 3 optical sensors are also in cluded in the intelligent mobile robot, which is driven by 2 D. C. motors, for clash avoidance in a way of direction toward an odour source. Throughout the experimental trails, it is confirmed that the intelligent mobile robot is capable of not only the odour recognition using artificial neural network algorithm, but also the tracking odour source using the step-by-step approach method. The preliminary results are promising that intelligent mobile robot, which has been developed, is applicable to service robot system for environmental monitoring, localization of odour source, odour tracking of hazardous areas etc.

• Journal of the Korean Society for Heat Treatment
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• v.9 no.3
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• pp.212-218
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• 1996

To clarify the influence of precipitation microstructure and inclusion on the monotonic tensile fracture behaviors in 2090 alloy aged at $180^{\circ}C$, the detailed measurement of hardness, tensile strength, elongation and the observation of scanning electron micrography, transmision electron micrography have been carried out. The transgranular shear ductile fracture has been observed in specimen quenched after solution treatment at $500^{\circ}C$ for 45min. While the under-aged specimen was fractured in both transgranular shear ductile and intergranular fracture mode, the fracture mode of peak-aged and over-aged alloy was predominantly intergranular fracture. The fracture behavior of each ageing condition was influenced by the change of precipitation microstructural features. In the case of peak-aged and over-aged alloys, the coarse and heterogeneous slip band caused by both shearable nature of the ${\delta}^{\prime}(Al_3Li)$ precipitates and PFZ along the high angle grain boundary aid the localization of deformation, resulting in low energy intergranular fracture. It was also estimated that the fractured T-type intermetallic phases (inclusion) and the equilibrium ${\delta}$(AlLi) phases which were formed at grain boundaries palyed an important role in promoting intergranular fracture mode.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.16 no.4 s.109
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• pp.428-436
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• 2006

The assessment of an urban site depends on the way whether it responds to multiple needs such as functionality, aesthetic and complex comfort of acoustic, thermal, lighting and air quality etc. This study aims to investigate the assessment of various urban soundscapes in the sense of acoustic amenity by the questionnaires. As a result, acoustic amenity assessment was influenced by the non-acoustic factors such as environment assessment of visual, thermal, air quality etc. In the sense of sound quality, urban environmental sound was interpreted as 3 factors of strength, evolution of time, spacial localization. So these factors would be considered in the new assessment method for acoustic amenity. And it was shown that the subjects tend to perceive the noise level less than $3{\sim}5dB\;L_{eqA}$ according to the urban landscapes under the similar noise exposure level.

• Journal of Power Electronics
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• v.16 no.3
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• pp.1163-1175
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• 2016

In recent years, microgrids have been the focus of considerable attention in distributed energy distribution. Microgrids contain a large number of power electronic devices that can potentially cause negative impedance instability. Harmonic impedance is an important tool to analyze stability and power quality of microgrids. Harmonic impedance can also be used in harmonic source localization. Precise measurement of microgrid impedance and analysis of system stability with impedances are essential to increase stability. In this study, we introduce a new square wave current injection method for impedance measurement and stability analysis. First, three stability criteria based on impedance parameters are presented. Then, we present a new impedance measurement method for microgrids based on square wave current injection. By injecting an unbalanced line-to-line current between two lines of the AC system, the method determines all impedance information in the traditional synchronous reference frame d-q model. Finally, the microgrid impedances of each part and the overall microgrid are calculated to verify the measurement results. In the experiments, a simulation model of a three-phase AC microgrid is developed using PSCAD, and the AC system harmonic impedance measuring device is developed.