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Effect of Helianthus tuberosus Juice Mixed with Dried Bitter Melon Juice on Hypoglycemic Function in Streptozotocin-Induced Diabetic Rats (당뇨유발 흰쥐에서 돼지감자 여주 혼합즙 음용에 의한 혈당저하 효과)

  • Yang, Yang;Kim, Yonghoon;Hwang, Eunhee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.46 no.8
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    • pp.903-909
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    • 2017
  • This study was conducted to develop Helianthus tuberosus (HT) juice mixed with dried bitter melon juice and assess its hypoglycemic effect in streptozotocin (STZ)-induced diabetic rats. HT juice mixed with 5.0% dried bitter melon juice was used in this study. Male Sprague-Dawley rats were divided into four groups (eight rats per group) and drunk each sample for 4 weeks: normal water [normal control (NC) group], STZ+normal water (STZ group), STZ+HT juice (HT group), STZ+HT juice mixed with 2.5% bitter melon juice (HT2.5 group), and STZ+HT juice mixed with 5.0% bitter melon juice (HT5.0 group). HT juice was diluted to 25% in distilled water and supplied to rats. Food intake, body weight gain, and food efficiency ratio were lower in the STZ group than in the NC group. HT, HT2.5, and HT5.0 groups showed higher parameters than the STZ groups. Water intakes were higher in the STZ group than in the NC group. After 3 weeks, HT, HT2.5, and HT5.0 groups showed lower parameters than the STZ group. After 1 week, blood glucose level of the STZ group ($476.7{\pm}22.8mg/dL$) was significantly higher than those of the HT group ($376.3{\pm}25.8mg/dL$), HT2.5 group ($405.2{\pm}35.1mg/dL$), and HT5.0 group ($342.8{\pm}29.7mg/dL$). After 4 weeks, blood glucose level of the STZ group were significantly higher than those of the HT, HT2.5, and HT5.0 group. Serum insulin levels of the HT group ($3.13{\pm}0.32ng/mL$), HT2.5 group ($3.40{\pm}0.23ng/mL$), and HT5.0 group ($3.48{\pm}0.43ng/mL$) were higher than that of the STZ group ($2.72{\pm}0.53ng/mL$). These results indicate that H. tuberosus juice mixed with dried bitter melon juice helps prevent or attenuate progression of diabetes in rats with STZ-induced diabetes.

Effects of Supplementation of Mixed Methanogens and Rumen Cellulolytic Bacteria on Biochemical Methane Potential with Pig Slurry (양돈슬러리를 이용한 혐기소화에서 미생물 첨가가 메탄발생에 미치는 영향)

  • Kim, Ji-Ae;Yoon, Young-Man;Jeong, Kwang-Hwa;Kim, Chang-Hyun
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.6
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    • pp.1049-1057
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    • 2012
  • The study investigated the biochemical methane potential (BMP) assay of pig slurry supplemented with mixed methanogens and cellulolytic bacteria to improve anaerobic digestion for methane production. For the BMP assay, 7 different microbial supplementation groups consisted of the cultures of mixed methanogens (M), Fibrobacter succinogenes (FS), Ruminococcus flavefaciensn (RF), R. albus (RA), RA+FS, M+RA+FS, and control. The cultures were added in the batch reactors with the increasing dose levels of 1% (0.5 mL), 3% (1.5 mL) and 5% (2.5 mL). Incubation for the BMP assay was carried out for 60 days at $38^{\circ}C$ using anaerobic digestate obtained from an anaerobic digester with pig slurry as inoculum. In results, 5% RF and RA+FS increased total biogas up to 8.1 and 8.4%, respectively, compared with that of control (p<0.05). All 5% microbial culture supplements significantly increased methane production up to 12.1~17.9% compared with that of control (p<0.05). Total solid (TS) and volatile solid (VS) digestion efficiencies showed no relationship to the increased supplementation levels of microbial cultures. After incubation, pH values in all treatment groups ranged between 7.527 and 7.657 indicating that methanogensis was not inhibited during the incubation. In conclusion, the results indicated that both hydrolysis and methanogenesis stages for methane production in anaerobic batch reactors were influenced by the supplemented microorganisms due to the chemical characteristics of pig slurry, but only the 5% supplementation level of all microbial culture supplements used in the experiment affected methane production.

Effect of Prenatal Fluoride on Bone Compositions of Rat (태생 전 불소투여로 인한 태생직후 백서 골조성 변화)

  • Kim, Hye-Young;Kwun, Hyun-Sook;Song, Keun-Bae;Hong, Suk-Jin
    • Journal of Korean society of Dental Hygiene
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    • v.2 no.2
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    • pp.175-186
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    • 2002
  • Fluoride has been one of the most widely studied caries-preventive agents. But the effect of prenatal administration had been controversies for many years. The results showed that there were no influence on reproductive rate of rats with administration of fluoride from 0 to 20 ppm during pregnancy(p>0.05). There was a trend towards slightly increased the mean ash weight in the 1, 5 and 20 ppm groups, as compared with the control group. However, there was no significant differences among groups (p>0.05). The contents of calcium, magnesium and phosphorus in the total bone were increased with the administrated fluoride concentration were increased, but there were no statistically significant differences among groups(p>0.05). The mean fluoride level of 1 ppm group was significantly higher than that of control group, but the concentrations of fluoride in total carcass pups of 5 and 20 ppm groups were significantly less than that of 1 ppm group(p>0.05). The results of this study indicate that the amount of fluoride transferred to the offspring, which may produce anticariogenic effects in the primary teeth of their effects in the primary teeth of their offspring.

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Anti-Inflammatory Effects of Volatile Flavor Extract from Herbal Medicinal Prescriptions Including Cnidium officinale Makino and Angelica gigas Nakai (천궁 및 당귀를 함유한 한방처방제 휘발성 향기추출물의 항염증 효과)

  • Leem, Hyun-Hee;Kim, Eun-Ok;Seo, Mi-Jae;Choi, Sang-Won
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.37 no.3
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    • pp.199-210
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    • 2011
  • This study was conducted to develop functional sources of herbal cosmetics for treatment of skin aging and inflammatory disorders using volatile flavor extracts of four different herbal medicinal prescriptions including Cnidium officinale Makino (COM), Angelica gigas Nakai (AGN), Mentha arvense L. (MAL), Artemisiae argyi Folium (AAF), Paeonia lactiflora Pall (PLP), Rehmanniae Radix Preparata (RRP), Scutellaria baicalensis Georgi (SBG), Panax ginseng C.A. Meyer (PGM), Glycyrrhiza uralensis Fisch (GUF). The volatile flavor extracts of four different herbal medicinal prescriptions (HH-1: COM, AGN, PLP, RRP, HH-2: COM, AGN, PLP, RRP, SBG, PGM, GUF, HH-3: COM, AGN, MAL, AAF, HH-4: COM, AGN, MAL, AAF, SBG, PGM, GUF) were extracted using SDE and their antioxidant and anti-inflammatory effects were measured by using DPPH radical and SLO, respectively. As a result, HH-2 showed moderate DPPH radical scavenging activity (68.24 %) and the strongest SLO inhibitory activity (83.96 %) at 100 ${\mu}g$/mL. Moreover, HH-2 of four different prescriptions significantly inhibited NO production on LPS-stimulated RAW 264.7 cells in a dose-dependent manner without considerable cell cytotoxicity at range of 2.0 ~ 50 ${\mu}g$/mL. Additionally, HH-2 also effectively suppressed the production of $PGE_2$ and IL-6, which are responsible for promoting the inflammatory process. Major volatile components of HH-2 were identified as eugenol, paeonol, butyl phthalide, ${\beta}$-eudesmol and butylidene dihydrophthalide by GC-MS analysis. Thus, these results suggest that HH-2 may be useful as a potential source of anti-inflammatory agents in herbal medicinal cosmetics.

Study for the Geochemical Reaction of Feldspar with Supercritical $CO_2$ in the Brine Aquifer for $CO_2$ Sequestration (이산화탄소의 지중저장 대염수층에서 과임계이산화탄소에 의한 장석의 지화학적 변화 규명)

  • Choi, Won-Woo;Kang, Hyun-Min;Kim, Jae-Jung;Lee, Ji-Young;Lee, Min-Hee
    • Economic and Environmental Geology
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    • v.42 no.5
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    • pp.403-412
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    • 2009
  • The objective of this study is to investigate the geochemical change of feldspar minerals by supercritical $CO_2$, which exists at $CO_2$ sequestration sites. High pressurized cell system (100 bar and $50^{\circ}C$) was designed to create supercritical $CO_2$ in the cell and the surface change and the dissolution of plagioclase and orthoclase were observed when the mineral surface reacted with supercritical $CO_2$ and water (or without water) for 30 days. The polished slab surface of feldspar was contacted with supercritical $CO_2$ and an artificial brine water (pH 8) in the experiments. The experiments for the reaction of feldspar with only supercritical $CO_2$ (without brine water) were also conducted. Results from the first experiment showed that the average roughness value of the plagioclase surface was 0.118 nm before the reaction, but it considerably increased to 2.493 nm after 30 days. For the orthoclase, the average roughness increased from 0.246 nm to 1.916 nm, suggesting that the dissolution of feldspar occurs in active when the feldspars contact with supercritical $CO_2$ and brine water at $CO_2$ sequestration site. The dissolution of $Ca^{2+}$ and $Na^+$ from the plagioclase occurred and a certain part of them precipitated inside of the high pressurized cell as the form of amorphous silicate mineral. For the orthoclase, $Al^{3+}$, $K^+$, and $Si^{+4}$ were dissolved in order and the kaolinite was precipitated. In the experiments without water, the change of the average roughness value and the dissolution of feldspar scarcely occurred, suggesting that the geochemical reaction of feldspars contacted with supercritical $CO_2$ at the environment without the brine water is not active.

Effects of acid-treatment conditions on the surface properties of the RBM treated titanium implants (산-처리 조건이 RBM처리한 티타늄 임플란트의 표면 특성에 주는 영향)

  • Lee, Han-Ah;Seok, Soohwang;Lee, Sang-Hyeok;Lim, Bum-Soon
    • Korean Journal of Dental Materials
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    • v.45 no.4
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    • pp.257-274
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    • 2018
  • The purpose of this study was to evaluate the effect of acid-treatment conditions on the surface properties of the RBM (Resorbable Blast Media) treated titanium. Disk typed cp-titanium specimens were prepared and RBM treatments was performed with calcium phosphate ceramic powder. Acid solution was mixed using HCl, $H_2SO_4$ and deionized water with 4 different volume fraction. The RBM treated titanium was acid treated with different acid solutions at 3 different temperatures and for 3 different periods. After acid-treatments, samples were cleaned with 1 % Solujet solution for 30 min and deionized water for 30 min using ultrasonic cleanser, then dried in the electrical oven ($37^{\circ}C$). Weight of samples before and after acid-treatment were measured using electric balance. Surface roughness was estimated using a confocal laser scanning microscopy, crystal phase in the surface of sample was analyzed using X-ray diffractometer. Surface morphology and components were evaluated using Scanning Electron Microscope (SEM) with Energy Dispersive X-ray spectroscopy (EDX) and X-ray Photoemission Spectroscopy (XPS). Values of the weight changes and surface roughness were statistically analyzed using Tukey-multiple comparison test (p=0.05). Weight change after acid treatments were significantly increased with increasing the concentration of $H_2SO_4$ and temperature of acid-solution. Acid-treatment conditions (concentration of $H_2SO_4$, temperature and time) did not produce consistent effects on the surface roughness, it showed the scattered results. From XRD analysis, formation of titanium hydrides in the titanium surface were observed in all specimens treated with acid-solutions. From XPS analysis, thin titanium oxide layer in the acid-treated specimens could be evaluated. Acid solution with $90^{\circ}C$ showed the strong effect on the titanium surface, it should be treated with caution to avoid the over-etching process.

Furrow Covering Effects with Rice Straw on Nutrient Discharge from Upland Soil Used for Red Pepper Cultivation (고추밭 고랑 볏짚피복에 의한 양분유출 특성)

  • Hong, Seung-Chang;Kim, Min-Kyeong;Jung, Goo-Buk;So, Kyu-Ho
    • Journal of the Korea Organic Resources Recycling Association
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    • v.24 no.1
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    • pp.11-19
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    • 2016
  • Excessive application of nutrient supplement on the upland soil may increase the amount of discharge to surrounding water systems. The chemical fertilizer (CF), cow manure compost (CMC), and pig manure compost (PMC) are used as a nutrient supplement for cultivation of red pepper. Rice straws are widely used as a soil covering material in order to reduce weed occurrence, to protect soil moisture, and to supply organic matter in upland soil. This study was conducted to evaluate the furrow covering effect with rice straw on nutrient discharge in upland soil used for red pepper cultivation. The experimental plots of nutrient supplement were consisted of CF, CMC, and PMC and the amount of nutrient application were as recommended amount after soil test for red pepper cultivation. Each nutrient supplement treatment plot has no furrow covering (CFC) as a control and furrow covering with rice straw (FCS), respectively. Furrow covering with rice straw (FCS) of CF treatment and CMC treatment reduced the amount of T-N(total nitrogen) discharge by $1.4kg\;ha^{-1}$, $2.1kg\;ha^{-1}$, respectively, compared to control. While the amount of T-P(total phosphorus) discharge of the furrow covering with rice straw of CF, CMC, and PMC increased by $2.1kg\;ha^{-1}$, $2.1kg\;ha^{-1}$, and $0.2kg\;ha^{-1}$, respectively, compared to control. The phosphorus and nitrogen content of straw were 0.4 % and 0.3 % respectively. In addition, in three week the phosphorus was eluted from the straw which soaked in distilled water. Thus, it was assumed that T-P discharging originated from rice straw which applied as a furrow covering material. The furrow covering with rice straw reduced weed occurrence compared to control. But production of fresh red pepper was not influenced significantly by furrow covering with rice straw. In conclusion, excessive furrow covering with rice straw could induce T-P discharge from upland soil used for red pepper cultivation. Further studies are needed to evaluate the appropriate amount of rice straw as a furrow covering material.

Characteristics of Ginseng Extract and Its Effects on Sensory Properties of Pork Sausage (인삼 추출물의 사포닌 조성 특성과 추출물 첨가가 포크소시지 관능 특성에 미치는 영향)

  • Park, Kyoung-Mi;Hwang, In-Ho
    • Food Science of Animal Resources
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    • v.26 no.4
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    • pp.418-425
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    • 2006
  • This study was conducted to evaluate extraction properties of crude saponin and ginsenosides, and their effects on sensory properties of emulsified pork sausage. Non-dried ginseng root was boiled in 0 (e.g., 100% distilled water), 20, 40, 60, 80 or 100% ethanol, and powdered by a freezing dry method. Weight of dried powder for the 0% ethanol extraction was 20% of initial non-dried ginseng weight, while $20{\sim}80%$ and 100% ethanol extractions resulted in approximately 15 and 10% of their initial weights, respectively. On the other hand, crude saponin content in the dried powder was linearly increased for a higher ethanol content where 100% ethanol extraction resulted in 123.52 mg/g. LC/MS analysis of crude saponin for quantifying ginsenosides showed that Rb1, Rb2 and Rc were significantly (p<0.05) higher levels for both 80 and 100% ethanol extractions. In the case of Rg1 ginsenoside, 60, 80 and 100% ethanol extractions resulted in significantly (p<0.05) higher levels. Emulsified pork sausages containing 0, 1 or 2% ginseng extracts were smoked or non-smoked and their sensory characteristics and preference were evaluated. Smoking process significantly (p<0.05) decreased juiciness and tenderness, but the treatment significantly (p<0.05) improved flavor and consumer preference. It was particularly noticed that a 2% addition of ginseng extract prevented the adverse effects of smoking process on juiciness and tenderness while the 2% addition significantly (p<0.05) improved consumer preference. The current results implied that addition of ginseng extract in emulsified pork sausage could improve sensory quality.

Preparation of Functional Healthy Drinks by Acanthopanax senticosus Extracts (가시오가피를 이용한 기능성 건강음료의 제조)

  • Sung, Mi-Sun;Jung, Hoe-Yune;Choi, Jun-Hyeok;Lee, Sung-Cheol;Choi, Bo-Hwa;Park, Sung Sun
    • Journal of Life Science
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    • v.24 no.9
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    • pp.959-966
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    • 2014
  • This study was carried out to develop a functional healthy drink using 60% ethanol of dried Acanthopanax senticosus stem extract (ASE). The preparation, physical activity, anti-oxidant activity, and sensory properties of ASE were investigated. The moisture, crude protein, crude lipid, and ash contents of dried ASE were $6.50{\pm}0.12%$, $5.89{\pm}0.16%$, $1.18{\pm}0.11%$, and $3.03{\pm}0.40%$ respectively. The 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was $87.42{\pm}1.63%$ at 1/10 folds diluted ASE. In total, 40 male ICR mice were divided into five groups including the control (PBS), positive control (Red ginseng 200 mg/kg/day), and ASE-treated groups at doses of 35, 70, and 140 mg/kg/day for five weeks, respectively. ASE was administrated orally one time per day for five weeks before treadmill exercises, and normal and positive controls were fed PBS and red ginseng extract. In the treadmill test, ASE-treated mice (140 mg/kg/day) could run 1.4 times longer than the control mice. Healthy drinks were prepared with the addition of ASE at levels of 0.97% or 0.49% (A, B, and C type). Among the healthy drinks, the B type (ASE, 0.97%) was revealed to have the highest level of taste and overall acceptability through a sensory evaluation. The brix and pH of the ASE health drink (B type) were 14.9 and 4.51, respectively. These results indicated that the dried stem of Acanthopanax senticosus could be used as a functional material in the health drink industry.

A Study on Fuel Quality Characteristics of F-T Diesel for Production of BTL Diesel (BTL 디젤 생산을 위한 F-T 디젤의 연료적 특성 연구)

  • Kim, Jae-Kon;Jeon, Cheol-Hwan;Yim, Eui-Soon;Jung, Choong-Sub;Lee, Sang-Bong;Lee, Yun-Je;Kang, Myung-Jin
    • Journal of the Korean Applied Science and Technology
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    • v.29 no.3
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    • pp.450-458
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    • 2012
  • In order to reduce the effects of greenhouse gas (GHG) emissions, the South Korean government has announced a special platform of technologies as part of an effort to minimize global climate change. To further this effort, the Korean government has pledged to increase low-carbon and carbon neutral resources for biofuel derived from biomass to replace fossil and to decrease levels of carbon dioxide. In general, second generation biofuel produced form woody biomass is expected to be an effective avenue for reducing fossil fuel consumption and greenhouse gas (GHG) emissions in road transport. It is important that under the new Korean initiative, pilot scale studies evolve practices to produce biomass-to-liquid (BTL) fuel. This study reports the quality characteristics of F-T(Fischer-Tropsch) diesel for production of BTL fuel. Synthetic F-Tdiesel fuel can be used in automotive diesel engines, pure or blended with automotive diesel, due to its similar physical properties to diesel. F-T diesel fuel was synthesized by Fischer-Tropsch (F-T) process with syngas($H_2$/CO), Fe basedcatalyst in low temperature condition($240^{\circ}C$). Synthetic F-T diesel with diesel compositions after distillation process is consisted of $C_{12}{\sim}C_{23+}$ mixture as a kerosine, diesel compositions of n-paraffin and iso-paraffin compounds. Synthetic F-T diesel investigated a very high cetane number, low aromatic composition and sulfur free level compared to automotive diesel. Synthetic F-T diesel also show The wear scar of synthetic F-T diesel show poor lubricity due to low content of sulfur and aromatic compounds compared to automotive diesel.