• 한국응용약물학회:학술대회논문집
• /
• 한국응용약물학회 1994년도 춘계학술대회 and 제3회 신약개발 연구발표회
• /
• pp.245-245
• /
• 1994

From the active fraction, cytotoxic constituents 1-5 were isolated and identified to be 2(S)-5,7-dihydroxy-8,2'-dimethoxyflavanone (1), wogonin (5,7-dihydroxy-8-methoxyflavone, 2), 5,7-dihydruxy-8,2'-dimethoxyflavone (3), 2(5) -5,7,2'-trihydroxy-8-methoxyflavanone (4), 2(S) - 5,2',5'-trihydroxy-7,8-dimethoxyflavanone (5), respectively, with those of specimens$\^$1-2)/

• Natural Product Sciences
• /
• 제12권4호
• /
• pp.205-209
• /
• 2006

The structures of flavonoids, 2(S)-5,7-dihydroxy-8,2'-dimethoxyflavanone (1), wogonin (2), 2(S)-5,7, 2'-trihydroxy-8-methoxyflavanone (3), and 2(S)-5,2',5'-trihydroxy-7,8-dimethoxyflavanone (4), isolated from Scutellaria indica were revised on the basis of 2D NMR spectroscopy, including to gCOSY, gHSQC, and gHMBC. Compounds 1-4 were tested in vitro protein tyrosine phosphatase 1B (PTP1B) inhibitory activity. Compounds 2 and 4 exhibited weak PTP1B inhibitory activity with $IC_{50}$ values of 208 and $337{\mu}M$, respectively.

• 한국약용작물학회지
• /
• 제14권4호
• /
• pp.212-216
• /
• 2006

황금 (Scutellaria baicalensis Gergi)에 함유된 항산화 억제활성 물질을 분리하고 항산화 효과에 대하여 조사한 결과는 다음과 같다. 1. 황금 500g을 100% MeOH로 추출하여 150g의 수율을 얻었으며, 다시 분획하여 Ethyl ether 13.2g과 n-BuOH 10.3g, Water 126.5g을 얻었다. 2. Ethyl ether 분획을 Column chromatography, TLC 및 HPLC를 이용하여 xanthine oxidase 억제활성을 가진 compound I을 분리하였다. 3. $^1H-NMR,\;^{13}C-NMR$, MS spectrometer 등 분광학적 방법을 이용하여 Compound I의 구조를 규명한 결과, flavonoid 물질로서 compound I은 구조식이 3,5,7-trihydroxy-2'-methoxy-flavanone로 확인되었다.

• Biomolecules & Therapeutics
• /
• 제21권2호
• /
• pp.121-125
• /
• 2013

Hesperetin (3',5,7-trihydroxy 4'-methoxyflavanone) and its glycoside hesperidin (hesperetin 7-rhamnoglucoside) in oranges have been reported to possess pharmacological effects related to anti-obesity. However, hesperetin and hesperidin have not been studied on suppressive effects on appetite. This study examined that hesperetin and hesperidin can stimulate the release of cholecystokinin (CCK), one of appetite-regulating hormones, from the enteroendocrine STC-1 cells, and then examined the mechanisms involved in the CCK release. Hesperetin significantly and dose-dependently stimulated CCK secretion with an $EC_{50}$ of 0.050 mM and increased the intracellular $Ca^{2+}$ concentrations ($[Ca^{2+}]_i$) compared to the untreated control. The stimulatory effect by hesperetin was mediated via the entry of extracellular $Ca^{2+}$ and the activation of TRP channels including TRPA1. These results suggest that hesperetin can be a candidate biomolecule for the suppression of appetite and eventually for the therapeutics of obesity.