• Journal of Sasang Constitutional Medicine
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• v.4 no.1
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• pp.193-212
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• 1992

Comparative hormones quantities on the blood analysis was carried out to investigate the hormones specific characters on the blood in four types of physical constitution. Thyroid-stimulating (TSH), triiodothyronine($T_3$), triiodothyronine uptake($T_3$ uptake), free triiodothyronine(free $T_3$), thyroxine($T_4$), free thyroxine (free $T_4$), thyroxine-binding globulin(TBG), adrenocorticotropic hormone(ACTH), luteinizing hormone(LH), testosterone were measured. The results obtained were summarized as follows: 1. The value of thyroid-stimulating hormone(TSH) on the constitution didn't shown significant difference, while the value of So-EUM-IN was increased and SO-YANG-IN was decreased. 2. The value of triiodothyronine($T_3$) on the constitution didn't shown significant difference, while the value of TAE-EUM-IN was decreased. 3. The value of triiodothyronine uptake($T_3$ uptake) on the constitution didn't shown significant difference, but between the value of TAE-EUM-IN and SO-EUM-IN, TAE-EUM-In and SO-YANG-IN showed significant difference. 4. The value of free triiodothyronine(free $T_3$) on the constitution didn't shown signifcant difference, but between the value of TAE-EUM-IN and SO-EUM-In, TAE-EUM-In and SO-YANG-IN showed significant difference. 5. The value of thyroxine($T_4$) on the constitution didn't shown significant difference, while the value of TAE-EUM-IN and SO-YANG-IN showed difference. 6. The value of free thyroxine(free $T_4$) on the constitution didn't shown significant difference, but between the value of TAE-EUM-IN and SO-YANG-IN showed significant difference. 7. The value of thyroxine-binding globulin(TBG) on the constitution didn't shown significant difference. 8. The value of adrenocorticotropic hormone(ACTH) on the constitution didn't shown significant difference. 9. The value of luteinizing hormone(LH) on the constitution didn't shown significant difference, but between the value of TAE-EUM-IN and SO-EUM-IN, TAE-EUM-IN and SO-YANG-IN showed significant difference. 10. The value of testosterone on the constitution didn't shown significant difference.

• Journal of Chest Surgery
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• v.25 no.11
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• pp.1209-1217
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• 1992

Triiodothyronine[T3] is an important regulator of the tissue metabolism, and may have potential use as an inotropic agent. The change of serum T3 level was studied in the pediatric age patients after cardiopulmonary by pass. Thyroid function was tested pre-operatively in 33 patients and total triiodothyronine[TT3] levels were serially measured during and after cardiopulmonary bypass[CPB]. After correction of dilutional effect, effects of various factors on the TT3 levels were analyzed. Abrupt fall of TT3 level was demonstrated at 15 minutes after CPB[80.1$\pm$5.9ng/dL] from the initial level of 133.6$\pm$5.3ng/dL, with some recovery at 6 hours[115.4$\pm$6.7ng/dL]. After then, gradual decrease occured reaching to the level of 77.2$\pm$4.2ng /dL at 24 hours. These falls of the TT3 after CPB were statistically significant. [p<0.01 ANOVA] Statistically significant correlation were found between the degree of hemodilution and TT3 concentration at 15 and 30 minutes after CPB[p<0.05]. But, other factors were analyzed to have no effect on TT3 levels. As the degree of the hemodilution increases, TT3 decreased less. This observation probably supports the fact that decrease of TT3 during CPB may be a result of sequestration of T3 into peripheral tissue. Although it was not statistically significant[p=0.08], the fall of TT3 was greater in the group to which plasmanate was added, than those not added. This finding seemed to be due to the increase of albumin and other thyroid-hormone-binding-proteins in the serum. Increase of these binding proteins might potentiate the sequestration of T3 into the liver and the kidney from serum, and as a consequence, decrese the serum TT3 level further.

• Nuclear Engineering and Technology
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• v.7 no.2
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• pp.127-133
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• 1975

For isotopic exchange labelling of 3,5,3'-triiodothyronine (T$_3$) and thyroxine (T$_4$) with radioiodide in the presence of molecular iodine, T$_3$:I$_2$ or T$_4$:I$_2$ molar ratios, pH, and reaction time are. considered to be important factors. A modified labelling and separation method is proposed in present paper, by which T$_3$-$^{125}$ I and T$_4$-$^{125}$ I can be obtained with the mean labelling yields of 45%, and 50%, respectively. The whole reaction products can be separated by adoption of thin-layer chromatography technique using silica gel plate and the solvent system composd of chloroform, methanol and ammonia.

• YAKHAK HOEJI
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• v.27 no.2
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• pp.117-124
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• 1983

For development of $EMIT-T_{3}$ assay, the conjugation of 3, 5, 3'-triiodothyroformic acid NHS ester to G6PDH was attempted in various reaction conditions. Up to now, the best conjugation condition was the ratio of $T_{3}$-NHS:G6PDH=100 in 25% carbitol-Tris buffer at pH 9, $0^{\circ}C$ during overnight. The obtained $T_{3}$-G6PDH conjugates usually had 20% residual enzyme activity which was inhibited by 40-70% with various $anti-T_{3}$ antibodies. Utilizing the conjugate I and an antibody (S2633G), a useful standard curve for $T_{3}$ assay was obtained in the range of 0 to 5ng/ml with 499 EMIT units of separation.

• Development and Reproduction
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• v.13 no.4
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• pp.313-320
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• 2009

The aim of this study was to test if 3,5,3'-triiodothyronine (T3) are involved in the osmoregulatory actions in euryhaline starry flounder Platichthys stellatus. We investigated osmoregulatory parameters ($Na^+,\;Cl^-$ and osmolality), blood cortisol and glucose in starry flounder acclimated to seawater (SW, 33 psu) and that were transferred and allowed to acclimate to freshwater (FW, 0 psu). Fish in SW were injected with T3 (5, 10, and $15{\mu}g$/g body weight) or vehicle (0.9% NaCl), and then transferred to FW. They were sampled 3 days after the transfer. With T3 at $10{\mu}g$/g, levels of plasma $Na^+$ and $Cl^-$ were significantly higher than in sham (only saline) and control fish (without hormone and saline). Osmolality was significantly higher after injection with T3 at 10 and $15{\mu}g$/g than in the control. However, T3 at $5{\mu}g$/g had no effect on hyper-osmoregulation. In this study, all dose of T3 induced a significant increases in plasma cortisol without glucose. These results suggest a positive hyper-osmoregulatory role of T3 in starry flounder to hypoosmotic environment, maybe a positive interaction of T3 with cortisol for maintenance of hyper-osmoregulatory ability.

• Journal of Pharmacopuncture
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• v.1 no.1
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• pp.53-76
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• 1997

In order to study the effect of aqua-acupuncture with Radix Angelicae gigantis extract solution by acuncture point. Radix Angelicae gigantis aqua-acupuncture was carried on every day for 3 days and 5 days 7 days respectively on the corresponding loci of Chondol(CV22) and non-acupuncture points (loci at the root of tail), after inducing hypothyroidism by thiourea administration and thereafter the contents of serum triiodothyronine$(T_3)$, thyroxine$(T_4)$, triiodothyronine uptake, free $T_3$, free $T_4$, total protein, albumin, creatinine, creatine, BUN, cholesterol, triglyceride, and total lipid were measured in hypothyroidism rats. The following results have been obtained : 1. $T_3$ was increased with statistical significance by Radix Angelicae gigantis aquq-acupuncture for 3 days, 5 days and 7 days Chondol(CV22) and non-acupuncture points as compared with the control group. 2. $T_3$ uptake was increased with statistical significance by Radix Angelicae gigantis aquq-acupuncture for 3 days, 5 days and 7 days to Chondol(CV22) as compared with the control group. 3. Free $T_4$ was increased with statistical significance by Radix angelicae gigantis aqua-acupuncture for 3 days, 5 days and 7 days to Chondol(CV22), and non-acupuncture points respectively as compared with the control group. 4. Albumin was decreased with statistical significance by Radix Angelicae gigantis aqua-acupuncture for 3 days, and 5 days and 7 days to Chondol(CV22), and non-acupuncture points respectively as compared with the control group. 5. Creatine was decreased with statistical significance by Radix Angelicae gigantis aqua-acupuncture for 3 days, and 5 days and 7 days to Chondol(CV22), and non-acupuncture points respectively as compared with the control group. 6. Cholesterol was decreased with statistical significance by Radix Angelicae gigantis aqua-acupuncture for 3 days, and 5 days and 7 days to Chondol(CV22) and non-acupuncture points respectively as compared with the control group. 7. Triglyceride was decreased with statistical significance by Radix Angelicae gigantis aqua-acupuncture for 3 days, and 5 days and 7 days to Chondol(CV22) and non-acupuncture points respectively as compared with the control group. 8. Total lipid was decreased with statistical significance by Radix Angelicae gigantis aqua-acupuncture for 3 days, and 5 days and 7 days to Chondol(CV22) and non-acupuncture points respectively as compared with the control group.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.6
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• pp.551-556
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• 2002

We have experimentally evaluated the content of thyroid hormones (THs), the growth and activity of larval rockfish (Sebastes schiegeli) born from parturient mother fish injected with 20 $\mu$g/g BW of 3,5,3-triiodo-L-thyronine ($T_{3}$). There was no difference of L-thyroxine ($T_{4}$) levels between controls (sham control and control) and $T_{3}$ groups in yolk-sac larvae just born from broodstock, while $T_{3}$level of $T_{3}$ group was significantly increased compared with controls. In addition, the both larval $T_{3}$ and $T_{4}$ levels in $T_{3}$ group were always higher than those of control during experimental period. Also, there were significant differences in the development of larvae from the two groups. The larval growth in total length and body weight according to the elapsed days after parturition showed the linear and curve equations, respectively, and the slopes of $T_{3}$ group were significantly higher than those of control. The survival rate of larvae in $T_{3}$ group was higher than that of control. Although the survival rate in $T_{3}$ group under the condition of starvation was significantly depressed compared with the control, larval swimming index in $T_{3}$ group was higher than control. Finally, these findings suggest that the exogenous $T_{3}$ could be transferred into hatched larvae in parturient rockfish by maternal injection, and subsequently the exogenous hormone could play some roles on physiological metabolism of larvae after parturition and may confer a distinct advantage to the fragile larvae during the early developmental stage.

• Journal of radiological science and technology
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• v.5 no.1
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• pp.55-62
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• 1982

Measurement of triiodothyronine($T_3$) concentration is useful for the diagnosic and treatment of thyroid gland diseases. Fundermental studies of measurement of $T_3$ concentration by radioimmunoassay were performed and values determined by commercially available kit, Coat-A-Count, Diagnostic Products Corporation. The optimal utilization of the radioimmunoassay in measuring $T_3$ concentrations is dependant not only on high quality performance of the assay, but also on their appropriate application to the clinical situation. These are several aspects that must be considered in every individual case. These include factors such as accurate pippeting in reagent preparation in the assay and through decanting to remove all visible moisture after incubation steps and so forth. In attempt to assess quality control of the radioimmunoassay of serum $T_3$, serum pools with high, medium, low $T_3$ concentrations were assayed for each of 5 samples. The results obtained with this study were as follow: 1. The coefficient of variation(C. V.) for the standard curve ranged between $0.2{\sim}3.5%$. 2. It was necessary that both incubation time and temperature should correctly be maintained all the in the assay performance. 3. The precison with the $T_3$ RIA procedure was good. 4. The measured values of serially diluted serum $T_3$ concentration with Ong/dl standard solution was proportional to the predicted values. However dilution curve of distilled water was not strait. 5. Calculated $T_3$ values of patient serum in normal group was $107.8{\pm}25.90ng/dl$ in male patient and $127.29{\pm}24.08ng/dl$ in female patient.

• The Korean Journal of Nuclear Medicine
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• v.9 no.1
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• pp.31-43
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• 1975

In an attempt to establish the diagnostic value of serum triiodothyronine and to correlate it with pathophysiologic mechanisms of thyroid hormones in various thyroid disorders, the author measured the serum triiodothyronine levels by means of radioimmunoassay and compared them with other thyroid function tests. This study was carried out in 152 cases with various thyroid functions; 28 cases as control, 51 cases of hyperthyroidism, 50 cases of euthyroidism and 23 cases of hypothyroidism. The results obtained were as follows: 1. The serum $T_3$ level in normal control group ranged between $131{\pm}34ng/dl$. 2. The serum $T_3$ levels ranged between $306{\pm}97ng/dl$ in hyperthyroidism $138{\pm}32ng/dl$ in euthyroidism and $60{\pm}27ng/dl$ in hypothyroidism. The significant differences between these groups were noted in this study. 3. In 5(9.9%) out of 51 cases with hyperthyoidism and 9(39.1%) of 23 cases with hypothyroidism, the serum $T_3$ were measured to be in normal range. Accordingly, the diagnostic value of the measurement of serum $T_3$ with hyperthyroidism was justifiable, but with hypothyroidism, it was less creditable than that of serum thyrotropin. 4. There was little significant difference between the diagnostic value of serum thyroxine and triiodthyronine levels in various thyroid disorders. However, $T_4/T_3$ ratio was decreased in patients with untreated hyperthyroidism because of more elevation of $T_3\;than\;T_4$. Consequently, the serum $T_3$ was more sensitive than $T_3$ in some thyroid disorders. 5. The serum $T_3$ level was much more sensitive and showed prompt shift in its level during the course of treatment on the patient with various thyroid disorders. And the measurement of serum $T_3$ was a good index for the evaluation of the thyroid function. From these results obtained, the measurement of serum $T_3$ by means of radioimmunoassay is a good way to understand the status of thyroid function with various thyroid disorders and evaluate the effects of the treatment given on these patients.

• The Korean journal of sports medicine
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• v.36 no.4
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• pp.214-220
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• 2018

Purpose: The purpose of this research is to study changes in pituitary hormone in anterior lobe and thyroid hormone before, after, and during recovery time in severe 100 km ultramarathon. Methods: Healthy middle-aged runners (age, $52.0{\pm}4.8$ years) participated in the test. Grade exercise test is done, and then blood is taken from those participants before and after completing 100 km ultramarathon at the intervals of 24 hours (1 day), 72 hours (3 days), and 120 hours (5 days) to analyze their luteinizing hormone (LH), follicle-stimulating hormone (FSH), thyroid stimulating hormone (TSH), triiodothyronine (T3), thyroxine (T4), and free thyroxine (Free T4). Results: For LH, it decreased more significantly at 100 km than pre-race. However, after 1 day result increased more than that of 100 km. At 3 days, it was significantly higher than pre-race and 100 km, recovering at 5 days. In terms of FSH, it decreased at 100 km, 1 day, and 3 days more than pre-race but recovered at 5 days. TSH was higher at 1 day and 5 days compared to pre-race. T3 was only higher at 100 km than pre-race. T4 was higher till 5 days at 100 km than pre-race. Free T4 increased more significantly at 100 km than pre-race. Conclusion: In terms of severe long distance running, LH and FSH which belong to hormone from anterior lobe as well as T3, T4, and Free T4 which belong to thyroid hormone showed their variation within the standard range. However, TSH showed abnormal increase from enhanced concentration of blood after marathon becoming hyper-activation even during the recovery period.