• Title/Summary/Keyword: 2PL

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Genetic Polvmorphism of Protease Inhibitor (Pl) in Korean Population (한국인 집단에서 Protease inhibitor(PI)의 유전적 다형)

  • 김현섭;강신성
    • The Korean Journal of Zoology
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    • v.38 no.2
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    • pp.294-298
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    • 1995
  • The genetic polymorphism of Protease inhibitor (Pl) in Korean population was investigated by using isoelectric focusing (IEF) in an ultra-narrow pH range,4.2-4.9, and immunoblottins. Three common alleles (Pl * Ml, Pl*2, Pl * M3) were observed and the frequencies for the alleles were Pl * M1=0.7843, Pl * M2=0.1613, Pl * M3=0.0323. In addition to the three common alleles, rare alleles (Pl *5, Pl * Z, Pl* H were detected at low-level frequency. Two unknovlm variants, which were not reported on previous studies in Korean population, were also found.

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알칼리 내성 Bacillus sp. YA-14 유래의 중복 Promotor를 이용한 재조합 Plasmid로부터의 Pectate Iyase의 발현

  • Park, Hee-Kyoung;Hahm, Byoung-Kwon;Yu, Ju-Hyun;Bai, Dong-Hoon
    • Microbiology and Biotechnology Letters
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    • v.25 no.6
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    • pp.571-579
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    • 1997
  • For the overproduction of pectate lyase (PL), the recombinant plasmid pl2BS fl which has strong promoter from alkali-tolerent Bacillus sp. YA-14 was used. In order to overexpress the pectate lyase by the action of overlapping strong promoter in pl2BS$\Delta$fl, 1.6 kb of PL gene was inserted into pl2BS$\Delta$fl to form pl2BS$\delta$f1-PL and the enzyme was expressed. But decreased expression efficiency of the PL gene was observed and it was due to the presence of the transcription terminator region on the upstream of the PL gene. The transcription terminator of the PL gene in pl2BS$\delta$f1-PL was removed and the resulting plasmid p12BS$\Delta$fl$\Delta$PL was formed. Bacillus subtilis 207-25 harboring the recombinant plasmid, p12BS$\Delta$fl$\Delta$PL, revealed increased expression efficiency with chloramphenicol induction when cat-86 was used as a reporter gene.

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Zur Valenz deutscher verbaler Somatismen mit der Komponente ${\lceil}hand{\rfloor}$ (독일어의 신체부위 "손" 관련 관용구의 결합가 연구)

  • Kim Soo-Nam
    • Koreanishche Zeitschrift fur Deutsche Sprachwissenschaft
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    • v.4
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    • pp.1-27
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    • 2001
  • 이 글의 목적은 독일어 신체어휘 관련 관용구들 가운데 ${\lceil}$Duden Band 11${\rfloor}$에 수록된 108개의 $\lceil$$\rfloor$ 관련 관용구를 대상으로 이들의 형태$\cdot$통사구조를 파악하고, 그들을 모형화하는 것이다. 우리는 연구 대상을 문장에서 결합가 보유어로서 술어의 기능을 하는 관용구에 한정했다. 우리는 $\lceil$$\rfloor$ 관련 관용구를 보충어의 수와 형태에 따라 크게 세 가지 부류, 즉 1가, 2가, 3가의 관용구로 구분하였다 보충어의 형태는 명사구(Sn, Sd, Sa)와 전치사구(pS)에 한정했으며 문장형태의 보충어, 예를 들어 부문장(NS)과 부정사문(Inf) 형태는 고려하지 않았다. 이들이 보충어로 간주될 수 있는지의 여부는 아직 더 많은 연구를 필요로 하기 때문에 다음 과제로 남겨두었다. 일차적으로 외적 결합가($\"{a}u{\beta}ere\;Valenz)$에 따라, 이차적으로는 내적 결합가(innere Valenz)에 따라 108개의 $\lceil$$\rfloor$ 관련 관용구를 분석한 결과 우리는 다음과 같은 형태$\cdot$통사적 문형을 얻을 수 있었다. $\cdot$ 1가 동사 관용구: 1) PL-Sn : (1) PL[VPL - Sa] - Sn (2) PL(VPL - pS) - Sn (3) PL[VPL - Sa - pS] - Sn (4) PL[VPL - pS - pS] - Sn Sondergruppen: PL[VPL - Sa - Inf] - Sn PL[VPL - pS - Inf] - Sn 2) PL - Sd: (1) PL[VPL - Sn] - Sd (2) PL[VPL - Sn(es) - pS] - Sd $\cdot$ 2가 동사 관용구1) PL - Sn - Sd: (1) PL[VPL - Sa] - Sn - Sd (2) PL[VPL - pS] - Sn - Sd (3) PL[VPL - Sa - pS) - Sn - Sd 2) PL - Sn - pS: (1) PL[VPL - Sa] - Sn - pS (2) PL[VPL - pS] - Sn - pS (3) PL(VPL - Sa - pS) - Sn - pS 3) PL[VPL - pS) - Sn -Sa $\cdot$ 3가 동사 관용구: (1) PL[VPL - pS] - Sn - Sd - Sa (2) PL[VPL - pS] - Sn - Sa - pS (3) PL[VPL - Sa] - Sn - Sd - pS 이러한 분류가 보여주듯이, 독일어에는 1가, 2가, 3가의 관용구가 있으며, 구조 외적으로 동일한 통사적 결합가를 갖는다 하더라도 구조 내적 성분구조가 다르다는 것을 알 수 있다. 우리는 이 글이 외국어로서의 독일어를 배우는 이들에게 독일어의 관용구를 보다 올바르게 이해할 수 있는 방법론적인 토대를 제공함은 물론, (관용어) 사전에서 외국인 학습자를 고려하여 관용구를 알기 쉽게 기술하는 데 도움을 줄 수 있기를 바란다.

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Inhibitory Effect of Persimmon Leaves on Melanin Synthesis and its Action Mechanism in B16F10 cells (시엽(枾葉)의 멜라닌 생성 억제와 작용기전에 관한 연구)

  • Jang, Du-Hyun;Yoo, Dong-Youl
    • The Journal of Korean Obstetrics and Gynecology
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    • v.22 no.2
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    • pp.43-59
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    • 2009
  • Purpose: This study was performed to determine the inhibitory effect of Persimmon Leaves extract (PL) on melanin synthesis in B16F10 melanoma cells B16F10. Methods: The inhibitory effects of PL on melanin synthesis were determined by in vitro assay. To elucidate inhibitory effects of PL on melanin synthesis, we determined the melanin release and melanin production in B16F10. And to investigate the action mechanism, we assessed the gene expression of tyrosinase, TRP-1, TRP-2, PKA, PKC${\beta}$, ERK-1, ERK-2, AKT-1, MITF in B16F10. Results: 1. PL inhibited melanin release, melanin production in B16F10. 2. PL inhibited tyrosinase activity in vitro and in B16F10. 3. PL suppressed the expression of tyrosinase, TRP-1, TRP-2 in B16F10. 4. PL suppressed the expression of PKA, PKC${\beta}$ in B16F10. 5. PL increased the expression of ERK-1, ERK-2, AKT-1 in B16F10. 6. PL suppressed the expression of MITF in B16F10. Conclusion: From these results, it may be concluded that PL is possesed of the antimelanogenetic effects.

Studies on the Biological Activities and Physicochemical Characteristics of Pigments Extracted from Korean Purple-Fleshed Potato (한국산 유색감자 색소의 이화학적 특성 및 생리 활성에 관한 연구)

  • Jeon, Tae-Woog;Cho, Yong-Sik;Lee, Sung-Hyeon;Cho, Soo-Muk;Cho, Hyeon-Mook;Chang, Kyu-Seob;Park, Hong-Ju
    • Korean Journal of Food Science and Technology
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    • v.37 no.2
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    • pp.247-254
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    • 2005
  • General composition, total anthocyan content, and antioxidative and antimicrobial activities of Korean purple. fleshed potatoes (varieties A-D based on coloring degree of cross sections) were investigated. Slight differences in composition content were observed among varieties. Color intensity analyzed by sensory evaluation test decreased in order of PL-14, 31, 28, 3, 17, and 6, Total anthocyanin contents differed significantly among varieties from 3 to 29 mg Per 100 g, and decreased in order of PL-28, 31, 14, 12, 5, and 3. PL-28, 31, and Jasim potatoes showed slightly higher antioxidant activities than ${\alpha}$-tocopherol. PL-28, 31, 6, and Jasim showed antimicrobial activities against three species each of Gram-positive and-negative bacteria, with highest activities observed against Bacillus subtilis and relatively high activities against E. coli.

Effect of Pyroligneous Liquor on Oxygen Radicals and Their Scavenger Enzymes in Liver of CD Rats (흰쥐 간조직의 활성산소 및 제거효소에 미치는 목초액의 영향)

  • Choi, Jin-Ho;Cho, Weon-Ki
    • Journal of Nutrition and Health
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    • v.40 no.2
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    • pp.111-117
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    • 2007
  • This study was designed to investigate the effects of pyroligneous liquor on oxygen radicals and their scavenger enzymes in the liver of Cri/Bgi CD rats (7 rats per group). Male rats were fed a basic diet prepared in our Lab., PL-0 (Control), PL-1, PL-25, PL-50 and PL-75 groups were Prepared to be 0%, 1%, 25%, 50% and 75%with distilled water using pyroligneous liquor (35% of Choa Co. Ltd.), and were administrated orally for 8 weeks. Superoxide radical contents in liver mitochondria and microsomes were significantly decreased to 12-14%, 11-15%, respectively, in these PL-25 and PL-50groups compared with the control group. Hydroxyl radical content in mitochondria and microsomes were markedly decreased to 12-20% and 17%, respectively, in these PL-25 and PL-50% groups compared with the control group. Hydrogen peroxide content in mitochondria and microsomes were significantly decreased about 15-12% and 22-20% in liver of PL-25 and PL-50 groups compared with the control group. Mn-SOD and Cu/Zn-SOD activities in liver of PL-25 and PL-50 groups were remarkably increased to 15-25%, 11-16%, respectively, compared with the control group. GPx activities in mitochondria and microsomes were significantly increased in the liver of PL-25 and PL-50 groups compared with the control group. CAT activities in mitochondria and cytosol were significantly increased to 12-14%, 15-27%, respectively, in the liver of PL-25 and PL-50 groups compared with the control group. These results suggest that long term administration orally of 25 and 50% pyroligneous liquor may effectively inhibit the formation of oxygen free radicals, and also scavenger enzyme activities significantly increase through the administration orally.

Time-resolved Photoluminescence Study of Seven-stacked InAs/InAlGaAs Quantum Dots

  • O, Jae-Won;Gwon, Se-Ra;Ryu, Mi-Lee;Jo, Byeong-Gu;Kim, Jin-Su
    • Proceedings of the Korean Vacuum Society Conference
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    • 2011.08a
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    • pp.265-265
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    • 2011
  • 자발형성법으로 InP (001) 기판에 성장한 InAs/InAlGaAs 양자점(QDs: quantum dots)의 광학적 특성을 PL (photoluminescence)와 TRPL (time-resolved PL)을 이용하여 분석하였다. InAs QDs 시료는 single layer InAs QDs (QD1)과 7-stacked InAs QDs (QD2)를 사용하였다. 두 시료 모두 저온 (10 K)에서 1,320 nm에서 PL 피크가 나타나고, 온도가 증가함에 따라 PL 피크는 적색편이 (red-shift)를 보였다. 양자점의 온도를 10 K에서 300 K까지 증가하였을 때 QD1은 178 nm 적색편이 하였으며, PL 스펙트럼 폭은 온도가 증가함에 따라 증가하였다. 그러나 QD2는 264 nm 적색편이를 보였으며 PL 스펙트럼의 폭은 QD1 시료와 반대로 온도가 증가함에 따라 감소하였다. QD2의 아주 넓은 PL 스펙트럼 폭과 매우 큰 적색편이는 InAs 양자점 크기의 변화가 QD1에 비해 훨씬 크기 때문이다. QD2의 경우 InAs 층수(layer number)가 증가함에 따라 InAs QD의 크기가 점차 증가하므로 QD 크기의 변화가 single layer인 QD1 시료보다 훨씬 크다. QD1의 PL 소멸은 파장이 증가함에 따라 점차 느려지다가 PL 피크 근처에서 가장 느린 소멸 곡선을 보이고, 파장이 더 증가하였을 때 PL 소멸은 점차 빠르게 소멸하였다. 그러나 QD2의 PL 소멸곡선은 파장이 증가함에 따라 점차 빠르게 소멸하였다. 이것은 QD2는 양자점 크기의 변화가 매우 크기 때문에 (lateral size=18~29 nm, height=2.8~5.9 nm) 방출파장이 증가함에 따라 양자점 사이의 파동함수의 겹침이 증가하여 캐리어의 이완이 증가하기 때문으로 설명된다. 온도에 따른 TRPL 결과는 두 시료 모두 10 K에서 150 K 까지는 소멸시간이 증가하였고, 150 K 이후부터는 소멸시간이 감소하였다. 온도가 증가함에 따라 소멸시간이 증가하는 것은 양자점에서 장벽과 WL (wetting layer)로 운반자(carrier)의 이동, 양자점들 사이에 열에 의해 유도된 운반자의 재분배 등으로 인한 발광 재결합으로 설명할 수 있다. 150 K 이상에서 소멸시간이 감소하는 것은 열적효과에 의한 비발광 재결합 과정에 의한 운반자의 소멸이 증가하기 때문이다. 온도에 따른 TRPL 결과는 두 시료 모두 150 K까지는 발광재결합이 우세하고, 150 K 이상에서 비발광재겹합이 우세하게 나타났다.

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Influence of InGaAs Capping Layers on the Properties of InAs/GaAs Quantum Dots (InAs/GaAs 양자점의 발광특성에 대한 InGaAs 캡층의 영향)

  • Kwon, Se Ra;Ryu, Mee-Yi;Song, Jin Dong
    • Journal of the Korean Vacuum Society
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    • v.21 no.6
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    • pp.342-347
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    • 2012
  • The optical properties of InAs quantum dots (QDs) grown on a GaAs substrates by migration enhanced molecular beam epitaxy method have been investigated by using photoluminescence (PL) and time-resolved PL measurements. The luminescence properties of InAs/GaAs QDs have been studied as functions of temperature, excitation laser power, and emission wavelength. The PL peak of InAs QDs capped with $In_{0.15}Ga_{0.85}As$ layer (QD2) measured at 10 K is redshifted about 80 nm compared with that of InAs QDs with no InGaAs layer (QD1). This redshift of QD2 is attributed to the increase in dot size due to the diffusion of In from the InGaAs capping layer. The PL decay times of QD1 and QD2 at 10 K are 1.12 and 1.00 ns taken at the PL peak of 1,117 and 1,197 nm, respectively. The reduced decay time of QD2 can be explained by the improved carrier confinement and enhanced wave function overlap due to increased QD size. The PL decay times for both QD1 and QD2 are independent on the emission wavelength, indicating the uniformity of dot size.

Temperature Dependence of Photoluminescence in $SiO_2$ (실리콘산화막의 광루미니센스 온도의존성에 관한 연구)

  • 이재희
    • Journal of the Korean Vacuum Society
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    • v.10 no.2
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    • pp.247-251
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    • 2001
  • Photoluminescence(PL) were observed from room temperature to 8K on $Si^+$-implanted silicon-oxide films. The PL intensities are increased from room temperature to 50~80K and decreased below 50K. The blue-shift occurs during the increasing of PL intensity. Also, temperature-dependent PL were measured at peak wavelengths. The first peak is the most sensitive to the measuring temperature. The experimental results are explained by quantum size effect of O rich defects or(and) Si rich defects rather than nanocrystal silicon.

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Optical Properties of InAs Quantum Dots Grown by Changing Arsenic Interruption Time (As 차단 시간 변화에 의한 InAs 양자점의 광학적 특성)

  • Choi, Yoon Ho;Ryu, Mee-Yi;Jo, Byounggu;Kim, Jin Soo
    • Journal of the Korean Vacuum Society
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    • v.22 no.2
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    • pp.86-91
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    • 2013
  • The optical properties of InAs quantum dots (QDs) grown on GaAs substrates grown by molecular beam epitaxy have been studied using photoluminescence (PL) and time-resolved PL measurements. InAs QDs were grown using an arsenic interruption growth (AIG) technique, in which the As flux was periodically interrupted by a closed As shutter during InAs QDs growth. In this study, the shutter of As source was periodically opened and closed for 1 (S1), 2 (S2), or 3 s (S3). For comparison, an InAs QD sample (S0) without As interruption was grown in a pure GaAs matrix for 20 s. The PL intensity of InAs QD samples grown by AIG technique is stronger than that of the reference sample (S0). While the PL peaks of S1 and S2 are redshifted compared to that of S0, the PL peak of S3 is blueshifted from that of S0. The increase of the PL intensity for the InAs QDs grown by AIG technique can be explained by the reduced InAs clusters, the increased QD density, the improved QD uniformity, and the improved aspect ratio (height/length). The redshift (blueshift) of the PL peak for S1 (S3) compared with that for S0 is attributed to the increase (decrease) in the QD average length compared to the average length of S0. The PL intensity, PL peak position, and PL decay time have been investigated as functions of temperature and emission wavelength. S2 shows no InAs clusters, the increased InAs QD density, the improved QD uniformity, and the improved QD aspect ratio. S2 also shows the strongest PL intensity and the longest PL decay time. These results indicate that the size (shape), density, and uniformity of InAs QDs can be controlled by using AIG technique. Therefore the emission wavelength and luminescence properties of InAs/GaAs QDs can also be controlled.