• Microbiology and Biotechnology Letters
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• v.52 no.1
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• pp.1-14
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• 2024

This study focused on isolating and identifying strains from the gut of Epinephelus akaara cultivated in aquaculture facilities on Jeju Island. The aim was to evaluate the potential of utilizing these strains as probiotics for industrial applications. A total of 129 strains were isolated from the gut of E. akaara and screened based on their ability to create a clear zone of 10 mm or more in a preliminary antimicrobial activity test. Twelve strains were selected for further analysis, including bile resistance, acid tolerance at different pH levels, antioxidant activity, antibiotic susceptibility, and biochemical characteristics using the API kit. Through these characteristic experiments, eight strains (G1, G3, G15, G21, B1, B2, B3, B5) were identified as having potential as probiotics. Among these, the B group strains (B1, B2, B3, B5) exhibited significantly higher activity compared to the G group strains (G1, G3, G15, G21). Based on the phylogenetic analysis of the 16S rRNA gene sequences of the selected microorganisms, the strains were named as follows: B1 strain as Lactobacillus paracasei B1, B2 strain as Lactococcus lactis B2, B3 strain as Lactobacillus plantarum B3, B5 strain as Lactococcus lactis subsp. hordniae B5, G1 strain as Bacillus licheniformis G1, G3 strain as Bacillus velezensis G3, G15 strain as Brevibacterium frigoritolerans G15, and G21 strain as Bacillus pumilus G21.

• Microbiology and Biotechnology Letters
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• v.10 no.2
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• pp.123-132
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• 1982

These experiments were conducted to investigate the enzymatic characteristics of the purified $\alpha$-amylase (F-2A) of Bacillus circulans F-2 and the digestion rate of various starches. 1. The molecular weight was estimated to be 93000 by SDS-polyacrylamide disc gel electrophoresis. The isoelectric point was about pH 5.0. The optimum pH for the enzyme action was 6.0-6.5 and the stable pH ranged pH 5.5-12.0. The optimum temperature was 6$0^{\circ}C$, and the purified $\alpha$-amylase was stable below 4$0^{\circ}C$. 2. The purified $\alpha$-amylase was activated by Mn$^{＋＋}$ and Co$^{＋＋}$, whereas it was inhibited by Ag$^{＋}$, HT$^{＋＋}$, Cu$^{＋＋}$ and Pb$^{＋＋}$. 3. The purified $\alpha$-amylase is considered to have no sulfhydryl residue essential for its catalytic activity. 4. Michaelis constant (Km) was 1.704 mg/$m\ell$. Activation energy between 25-4$0^{\circ}C$ was 12.297 Kcal/mole, and between 40-6$0^{\circ}C$, it was 7.831 Kcal/mole. 5. The hydrolysis product from soluble starch, amylose and amylopectin in the early stage of hydrolysis was G$_{6}$, and as hydrolysis proceeds, G$_4$and G$_2$appeared. 6. Products from each oligosaccarides are as follows: G$_4$longrightarrow G$_2$＋ G$_2$,G$_3$ ＋G$_1$,G$_{5}$longrightarrow G$_4$＋G$_1$,G$_{6}$longrightarrowG$_4$＋ G$_2$,G$_{7}$ G$_4$,G$_{8}$longrightarrow G$_4$＋G$_4$, 7. On raw potato starch, raw sago starch and raw yam starch, the purified enzyme exhibited a remarkably high digestion rate than Porcine pancreatic amylase and Streptococcus bovis amylase.

• Journal of the Korea Society of Computer and Information
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• v.24 no.7
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• pp.61-69
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• 2019

In this paper, a classification method of random graph models is proposed and it is based on centralities of the random graphs. Similarity between two random graphs is measured for the classification of random graph models. The similarity between two random graph models $G^{R_1}$ and $G^{R_2}$ is defined by the distance of $G^{R_1}$ and $G^{R_2}$, where $G^{R_2}$ is a set of random graph $G^{R_2}=\{G_1^{R_2},...,G_p^{R_2}\}$ that have the same number of nodes and edges as random graph $G^{R_1}$. The distance($G^{R_1},G^{R_2}$) is obtained by comparing centralities of $G^{R_1}$ and $G^{R_2}$. Through the computational experiments, we show that it is possible to compare random graph models regardless of the number of vertices or edges of the random graphs. Also, it is possible to identify and classify the properties of the random graph models by measuring and comparing similarities between random graph models.

• Journal of the Chungcheong Mathematical Society
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• v.22 no.2
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• pp.211-216
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• 2009

Let G be a Denjoy domain and let G' a Denjoy proper subdomain of G and homeomorphic to G. We consider conformal re-imbeddings of G' into G. Let G and G' are N-connected. We know that if N = 2, there is a re-imbedding f of G' into G such that G - cl(f(G')) has an interior point. In this note, we obtain the following theorem. If $N{\geq}3$, G has a Denjoy proper subdomain G' such that, for any re-imbeddings f of G' into G, G - cl(f(G') has no interior point.

• Journal of Radiation Industry
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• v.8 no.1
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• pp.35-42
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• 2014

This study was conducted to analyze the protective capacity of cyanidin-3-glucoside (C3G), which is rich in mulberry and blackberry as an anthocyanin pigment. In this study, we found that treatment with C3G significantly reduced ROS production in hydrogen peroxide $(H_2O_2)-treated$ HepG2 cells in a dose-dependent manner. In addition, treatment with C3G significantly increased the cell viability in a dose-dependent manner in $H_2O_2-treated$ HepG2 cells. Moreover, treatment with C3G dose-dependently decreased the release of LDH and activation of caspase-3 in HepG2 cells treated with $H_2O_2$. Furthermore, the DNA damage in $H_2O_2-treated$ HepG2 cells was decreased by C3G treatment when compared with the control group in a dose-dependent manner. Additionally, treatment with C3G recovered the activity of antioxidant enzymes such as superoxide dismutase and catalase in $H_2O_2-treated$ HepG2 cells. To summarize, these results suggest that C3G protects cells from $H_2O_2-induced$ oxidative damage by activating antioxidant enzymes.

• Honam Mathematical Journal
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• v.39 no.2
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• pp.247-258
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• 2017

For a quadrilateral P, we consider the centroid $G_0$ of the vertices of P, the perimeter centroid $G_1$ of the edges of P and the centroid $G_2$ of the interior of P, respectively. It is well known that P satisfies $G_0=G_1$ or $G_0=G_2$ if and only if it is a parallelogram. In this note, we investigate various quadrilaterals satisfying $G_1=G_2$. As a result, for example, we show that among circumscribed quadrilaterals kites are the only ones satisfying $G_1=G_2$. Furthermore, such kites are completely classified.

• The Transactions of the Korea Information Processing Society
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• v.6 no.8
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• pp.2124-2132
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• 1999

Given a graph G=(V,E), Ld(2,1)-labeling of G is a function f : V(G)$\longrightarrow$[0,$\infty$) such that, if v1,v2$\in$V are adjacent, $\mid$ f(x)-f(y) $\mid$$\geq$2d, and, if the distance between and is two, $\mid$ f(x)-f(y) $\mid$$\geq$d, where dG(,v2) is shortest distance between v1 and in G. The L(2,1)-labeling number (G) is the smallest number m such that G has an L(2,1)-labeling f with maximum m of f(v) for v$\in$V. This problem has been studied by Griggs, Yeh and Sakai for the various classes of graphs. In this paper, we discuss the upper-bound of ${\lambda}$ (G) for a chordal graph G and that of ${\lambda}$(G') for a permutation graph G'.

• Korean Journal of Applied Biomechanics
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• v.16 no.2
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• pp.45-53
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• 2006

The purpose of this study was to analyze exercise related mechanical variables according to performance levels and Kumi-kata types in elite judo players (G1) and non-elite judo players, namely university players (G2). To achieve this purpose, three players in G1 whose main special skill was Morote-Seoinage and three university judo players(G2) was selected as comparative group. Then they were examined for distinguishing A and B types of Kumi-kata. Analyzed variables were the time required to show skills, knee degree, elbow degree. After analysing this study, conclusions were derived as follows. 1. In total necessary time of showing skills according to group of Kumi-kata type, G2 was longer than G1 in both A type (20.9%) and B type (23.7%). In necessary time of phase, in only 3P, G1 was shorter than G2 in A type (50%) and B type (75%). There was no difference in time required of 1P and 2P according to Kumi-kata type of group and in only 3P, B type was shorter than A type in both B type (75%) of G1 and B type (50%) of G2 2 There was no difference in elbow degree of offensive arm according to group of Kumi-kata type, however in A and B types, G1 could use skills by extending in kake phase, but G2 could use skills by bending. Elbow degree of offensive arm according to Kumi-kata type of group showed difference in E1. and F2 of G1. A and B types of G1 extended elbow degree in Kake phase, but G2 bent elbow degree so exercise program which could movable range extensively in Kake phase is needed.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.22 no.1
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• pp.127-134
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• 2022

A modified folded monopole slot antenna for 3G WCDMA (1.91 ~ 2.17 GHz), 4G LTE (2.17 ~ 2.67 GHz), 3.5 GHz 5G (3.42 ~ 3.7 GHz) and Wi-Fi dual band (2.4 ~ 2.484 GHz / 5.15 ~ 5.825 GHz) was proposed for the first time. The proposed antenna is designed and fabricated on a FR-4 substrate with dielectric constant 4.3, thickness of 1.6 mm, and size of 35 × 60 mm². The measured impedance bandwidth of the proposed antenna is 2910 MHz(1.84 ~ 4.75 GHz) and 930 MHz(5.11 ~ 6.04 GHz), antenna gain in each frequency band is from 1.811 to 3.450 dBi. In particular, it was possible to obtain a commercially suitable omni-directional radiation pattern in all frequency bands of interest.

• Microbiology and Biotechnology Letters
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• v.23 no.6
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• pp.647-651
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• 1995

A tylosin-hyperproductive mutant of S. fradiae MNU20 was isolated among 3500 strains obtaincd from either MNNG- or UV-treated Streptomyces fradiae NRRL2702. The composition of optimal medium for tylosin production was formulated as followed as: 4 g soluble starch, 1 g glucose, 1 g corn steep liquor, 7.5 ml soy bean oil, 0.2 g KH$_{2}$PO$_{4}$, 1 g Na$_{2}$S$_{2}$O$_{3}$$\cdot $5H$_{2}$O, 2 g CaCO$_{3}$, 2 g NaCl, 0.001 g CoCl$_{2}$$\cdot $6H$_{2}$O in 1 liter of distilled water. With the optimal medium, S. fradiae MNU20 was able to produce 159 mg tylosin (g biomass)$^{-1}$, indicating that tylosin productivity of Streptomyces fradiae NRRL2702 was increased 14 times higher by mutation. When the effect of valine, succinate, and natural zeolite on tylosin production was investigated by using the optimal medium, these substances essentially enhanced tylosin production and their addition time during culture period appeared to be critical for the increase of tylosin production.