• Title/Summary/Keyword: 2D plate

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Electrophoretic Patterns of Sarcoplasmic Proteins in Mid-West Korean Sea Fishes by Thin Layer Polyacrylamide Gel Isoelectrofocusing (등전점 전기영동에 의한 서해산 해산어의 근형단백질의 전기영동에 관한 연구)

  • 김종배;최선남;이근우;정용현
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.20 no.5
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    • pp.455-460
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    • 1991
  • In order to identify hard distinct 12 fish species(shiba shrimp Metapenaeus joyneri, fleshy shrimp Penaeus orientalis, ridgetail prawn Palaemon carinicauda, yellow croaker Pseudosciaena manchurica, croaker Niber albiflora, Colichthyes fragilis, brown sole Limanda herzensteini, frog flounder Pleuronichthys cornutrs, Areliscus rhomaleus, stone flounder Kareius bicoloratus, harvest fish Pampus argenteus, flag fish Goniistius zonatus) by seeing with naked eye in Kunsan coastal area, sarcoplasmic protein in the supernatant was used for isoelectric focusing. For getting supernatant, fish muscle tissue was blended with two times deionized water and centrifuged (at $4^{\circ}C$, 12,000rpm for 15min). Isoelectric focusing of sarcoploasmic protein carried out on a LKB Multiphor II using polyacrylamide gel plate (2mm thickness, pH $3.5~10^{\circ}C$, pH 5~8 gradient, at $10^{\circ}C$ for 1.5, 3 hours). In case of uncertain protein pattern, pH gradient was modified to narrow pH gradiet, and excuted 2-D electrophoresis using conventional polyacrylamide gel electrophoresis. Most of fishes except yellow croaker and Collichthyes fragilis were distingushed by isoelectric focusing. The protein maps of 2-D electrophoresis for analyzing two protein bands at aimilar positions(pH 5, 6) between the two fish species showed the diffeences of the estimated molecular weights, 11,700(pH5.0) and 87,000(pH6.0)

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Cryogenic milling for the fabrication of high Jc MgB2 bulk superconductors

  • Kim, D.N.;Kang, M.O.;Jun, B.H.;Kim, C.J.;Park, H.W.
    • Progress in Superconductivity and Cryogenics
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    • v.19 no.2
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    • pp.19-24
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    • 2017
  • Cryogenic milling which is a combined process of low-temperature treatment and mechanical milling was applied to fabricate high critical current density $(J_c)MgB_2$ bulk superconductors. Liquid nitrogen was used as a coolant, and no solvent or lubricant was used. Spherical Mg ($6-12{\mu}m$, 99.9 % purity) and plate-like B powder (${\sim}1{\mu}m$, 97 % purity) were milled simultaneously for various time periods (0, 2, 4, 6 h) at a rotating speed of 500 rpm using $ZrO_2$ balls. The (Mg+2B) powders milled were pressed into pellets and heat-treated at $700^{\circ}C$ for 1 h in flowing argon. The use of cryomilled powders as raw materials promoted the formation reaction of superconducting $MgB_2$, reduced the grain size of $MgB_2$, and suppressed the formation of impurity MgO. The superconducting critical temperature ($T_c$) of $MgB_2$ was not influenced as the milling time (t) increased up to 6 h. Meanwhile, the critical current density ($J_c$) of $MgB_2$ increased significantly when t increased to 4 h. When t increased further to 6 h, however, $J_c$ decreased. The $J_c$ enhancement of $MgB_2$ by cryogenic milling is attributed to the formation of the fine grain $MgB_2$ and a suppression of the MgO formation.

Effect of Gamma Irradiation on Shelf-life Extension and Sensory Characteristics of Dak-galbi (Marinated Diced Chicken) during Accelerated Storage

  • Yoon, Yo-Han;Cho, Won-Jun;Park, Jin-Gyu;Park, Jae-Nam;Song, Beom-Seok;Kim, Jae-Hun;Byun, Myung-Woo;Kim, Cheon-Jei;Sharma, Arun K.;Lee, Ju-Woon
    • Food Science of Animal Resources
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    • v.29 no.5
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    • pp.573-578
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    • 2009
  • This study examined the effect of gamma irradiation on shelf-life extension and sensory characteristics of dak-galbi. Commercial dak-galbi sauce was gamma-irradiated at 0, 5, 10, 15, 20, 25, and 30 kGy. The dak-galbi sauce (200 g) was then added to diced chicken (800 g) for cooking, and the cooked dak-galbi samples in vacuum bags were stored at $35^{\circ}C$ for 5 d. Dak-galbi samples were analyzed on d 0, 1, 2, 3, 4, and 5 for microbial analysis (plate count agar), thiobarbituric acid (TBARS) and volatile basic nitrogen (VBN) measurements, and on d 0 for sensory evaluation. On d 0, total bacterial populations were below detection limit after dak-galbi marinated with the gamma-irradiated (${\geq}15\;kGy$) sauces were cooked, and the samples marinated with higher dose irradiated dak-galbi sauce had lower (p<0.05) bacterial populations during storage. TBARS values of the dak-galbi samples marinated with non-irradiated sauce were not different ($p{\geq}0.05$) with those marinated with irradiated sauces on d 0, and the TBARS values increased (p<0.05) during storage at $35^{\circ}C$, regardless of irradiation dose. In the VBN analysis, there was no difference ($p{\geq}0.05$) in VBN values among irradiation doses on d 0, but VBN values decreased (p<0.05) as irradiation dose increased during storage. Moreover, there were no significant differences ($p{\geq}0.05$) in sensory characteristics among irradiation doses. These results indicate that use of gamma irradiation on dak-galbi sauce may be useful in shelf-life extension without compromising the sensory characteristics of dak-galbi.

Hot Corrosion Behavior of Inconel Alloys and Incoloy 800H in Molten LiCl-Li2O Salt (LiCl-Li2O 용융염에서 Inconel 합금 및 Incoloy 800H의 고온 부식거동)

  • Lim, Jong-Ho;Choi, Jeong-Mook
    • Korean Journal of Materials Research
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    • v.23 no.2
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    • pp.128-134
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    • 2013
  • A study on the corrosion behavior of Inconel alloys and Incoloy 800H in molten salt of LiCl-$Li_2O$ was investigated at $650^{\circ}C$ for 24-312 hours in an oxidation atmosphere. The order of the corrosion rate was Inconel 600 < Inconel 601 < Incoloy 800H < Inconel 690. Inconel 600 showed the best performance suggesting that the content of Fe, Cr and Ni are the important factor for corrosion resistance in hot molten salt oxidation conditions. The corrosion products of Inconel 600 and Inconel 601 were $Cr_2O_3$ and $NiFe_2O_4$, In case of Inconel 690, a single layer of $Cr_2O_3$ was formed in the early stage of corrosion and an outer layer of $NiFe_2O_4$ and inner layer of $Cr_2O_3$ were formed with an increase of corrosion time. In the case of Incoloy 800H, $Cr_2O_3$ and $FeCr_2O_4$ were observed. Most of the outer scale of the alloys was observed to be spalled from the results of the SEM analysis and the unspalled scale which adhered to the substrate was composed of three layers. The outer layer, the middle one, and the inner one were Fe, Cr, and Ni-rich, respectively. Inconel 600 showed localized corrosion behavior and Inconel 601, 690 and Incoloy 800H showed uniform corrosion behavior. Ni improves the corrosion resistance and too much Cr and/or Fe content deteriorates the corrosion resistance.

Mixed Mode Analysis using Two-step Extension Based VCCT in an Inclined Center Crack Repaired by Composite Patching (복합재료 팻칭에 의한 중앙경사균열에서 2단계 확장 가상균열닫힘법을 사용한 혼합모우드해석)

  • Ahn, Jae-Seok;Woo, Kwang-Sung
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.32 no.1A
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    • pp.11-18
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    • 2012
  • This paper deals with the numerical determination of the stress intensity factors of cracked aluminum plates under the mixed mode of $K_I$ and $K_{II}$ in glass-epoxy fiber reinforced composites. For the stress intensity factors, two different models are reviewed such as VCCT and two-step extension method. The p-convergent partial layerwise model is adopted to determine the fracture parameters in terms of energy release rates and stress intensity factors. The p-convergent approach is based on the concept of subparametric element. In assumed displacement field, strain-displacement relations and 3-D constitutive equations of a layer are obtained by combination of 2-D and 1-D higher-order shape functions. In the elements, Lobatto shape functions and Gauss-Lobatto technique are employed to interpolate displacement fields and to implement numerical quadrature. Using the models and techniques considered, effects of composite laminate configuration according to inclined angles and adhesive properties on the performance of bonded composite patch are investigated. In addition to these, the out-of-plane bending effect has been investigated across the thickness of patch repaired laminate plates due to the change of neutral axis. The present model provides accuracy and simplicity in terms of stress intensity factors, stress distribution, number of degrees of freedom, and energy release rates as compared with previous works in literatures.

Bactericidal Efficacy of a Fumigation Disinfectant Containing Paraformaldehyde Against Salmonella Typhimurium

  • Cha, Chun-Nam;Son, Song-Ee;Yoo, Chang-Yeul;Park, Eun-Kee;Jung, Ji-Youn;Kim, Suk;Lee, Hu-Jang
    • Journal of Food Hygiene and Safety
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    • v.31 no.3
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    • pp.216-221
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    • 2016
  • This study was performed to evaluate the bactericidal efficacy of a fumigation disinfectant containing 35% paraformaldehyde against Salmonella Typhimurium (S. Typhimurium). In this study, the efficacy test of a fumigant against S. Typhimurium was carried out according to French standard NF T 72-281. The S. Typhimurium working culture suspension number (N value), all bacteria numbers on the carriers exposed to the fumigant (n1, n2, and n3), the number of bacterial suspensions by the pour plate method (N1), the number of bacterial suspensions by the filter membrane method (N2), and the mean number of bacteria recovered on the control carriers (T value), were obtained from the preliminary test. In addition, the reduction number of S. Typhimurium exposed to the fumigant (d value) was calculated using the T value, the mean number of bacteria in the recovery solution (n'1) and the mean number of bacteria on carriers plated in agar (n'2). The N value was $5.5{\times}10^8$ colony forming units (CFU)/mL, and n1, n2, and n3 were higher than 0.5N1, 0.5N2 and 0.5N1, respectively. Additionally, the T value was $3.5{\times}10^6CFU/carrier$. In terms of the bactericidal effect of the fumigant, the d value was 5.25. According to the French standard for fumigants, the d value for an effective bactericidal fumigant should be greater than 5. The results indicated that the fumigant containing 35% paraformaldehyde had an efficient bactericidal activity against S. Typhimurium, and, therefore, can be used to disinfect food materials and kitchen appliances contaminated with foodborne bacteria.

Corrosion Behavior of Pyro-Carbon in Hot Lithium Molten Salt Under an Oxidation Atmosphere (산화성 고온 리튬용융염계 분위기에서 Pyro-Carbon의 부식거동)

  • Lim, Jong-Ho;Choi, Jeong-Mook
    • Korean Journal of Materials Research
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    • v.23 no.2
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    • pp.123-127
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    • 2013
  • The electrolytic reduction of a spent oxide fuel involves liberation of the oxygen in a molten LiCl electrolyte, which is a chemically aggressive environment that is too crosive for typical structural materials. Therefore, it is essential to choose the optimum material for the process equipment for handling a molten salt. In this study, the corrosion behavior of pyro-carbon made by CVD was investigated in a molten LiCl-$Li_2O$ salt under an oxidation atmosphere at $650^{\circ}C$ and $750^{\circ}C$ for 72 hours. Pyro-carbon showed no chemical reactions with the molten salt because of its low wettability between pyro-carbon and the molten salt. As a result of XRD analysis, pyro-carbon exposed to the molten salt showed pure graphite after corrosion tests. As a result of TGA, whereas the coated layer by CVD showed high anti-oxidation, the non-coated layer showed relatively low anti-oxidation. The stable phases in the reactions were $C_{(S)}$, $Li_2CO_{3(S)}$, $LiCl_{(l)}$, $Li_2O$ at $650^{\circ}C$ and $C_{(S)}$, $LiCl_{(l)}$, $Li_2O_{(S)}$ at $750^{\circ}C$. $Li_2CO_{(S)}$ was decomposed at $750^{\circ}C$ into $Li_2O_{(S)}$ and $CO_{2(g)}$.

Characteristics for Gait of the Induced Equinus in Normal Subjects (정상인에서 유도된 첨족에 따른 신체 보행의 특성)

  • Woo, Byung-Hoon
    • Korean Journal of Applied Biomechanics
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    • v.24 no.4
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    • pp.435-443
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    • 2014
  • The purposes of this study was to investigate the physical compensation for gait on induced equinus in normal subjects. Ten subjects were participate in the experiment (age: $23.8{\pm}2.8yrs$, height: $177.3{\pm}4.3cm$, weight: $70.8{\pm}4.6kg$). The study method adopted 3D analysis with six cameras and ground reaction force with two force-plate. Induced equinus were classify as gait pattern on unilateral and bilateral equinus. The results were as follows; In displacement of COM, medio-lateral and anterior-posterior COM were no significant, but in vertical COM, unilateral equinus gait was higher than bilateral equinus gait. In displacement hip joint, left hip joint was more extended in FC1 and FC2 during unilateral equinus gait. In displacement knee joint, left knee joint was more extended in FC2, right knee joint was more extended in all event during unilateral equinus gait. In trunk tilt, unilateral equinus gait was more forward tilt in TO1 and TO2. ROM of each joint was no significant. In Displacement of pelvic tilt angle, X axis of unilateral equinus gait was more increase than bilateral equinus gait at FC2, TO2 and MS2. Y axis of unilateral equinus gait was more increase than bilateral equinus gait at MS1, FC2 and MS2. Z axis was no significant in both equinus gait. In GRF, right Fx and Fy were no significant in both equinus gait, Fz was more bigger vertical force in bilateral equinus gait. Left Fx was more bigger internal force in unilateral equinus gait, Fy and Fz were no significant in both equinus gait.

The Preventive Effect of Bacillus polyfermenticus KJS-2 and Angelica gigas Nakai Extract on Triton WR-1339-induced Hyperlipidemia (Bacillus polyfermenticus KJS-2와 참당귀 추출물의 triton WR-1339 유발 고지혈증에 대한 예방효과)

  • Kim, Kang Min;Kim, Bo Seul;Kang, Jae Seon
    • Journal of Life Science
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    • v.28 no.6
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    • pp.726-732
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    • 2018
  • The purpose of this study was to investigate the effects of Bacillus polyfermenticus KJS-2 (B. polyfermenticus KJS-2) and Angelica gigas Nakai extracts (AGNE) on hyperlipidermia. The purity of the major decursin and decursinol angelate (D/DA) in the AGNE were analyzed at 78%. Increased concentrations of AGNE (0.1-20 mg/ml) showed a higher 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase inhibition activity. Endospore-forming B. polyfermenticus KJS-2 ($1{\times}10^9CFU/ml$) exhibited good bile tolerance (0.3 mm) on an agar plate. An animal study was carried out using different groups, including a normal control, positive control (atorvastatin), negative control (triton WR-1339), AGNE group, B. polyfermenticus KJS-2 group, AGNE + B. polyfermenticus KJS-2 group, and Atorvastatin + AGNE + B. polyfermenticus KJS-2 group to determine the effect of hyperlipidemia. There were no significant changes in body weight, kidney weight, or liver weight except for the liver weight of the triton WR-1339-treated group. Groups with AGNE and B. polyfermenticus KJS-2 had increased HDL-cholesterol and decreased total cholesterol and triglycerides. The liver histopathological results also showed that all AGNE and B. polyfermenticus KJS-2-treated groups contained lower fat accumulation in the liver tissues. The findings of this study verified that AGNE and Endospore-forming B. polyfermenticus KJS-2 combination materials have a hyperlipidemic effect.

Development of a Control System for Automated Line Heating Process by an Object-Oriented Approach

  • Shin, Jong-Gye;Ryu, Cheol-Ho;Choe, Sung-Won
    • Journal of Ship and Ocean Technology
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    • v.6 no.4
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    • pp.1-12
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    • 2002
  • A control system for an automated line heating process is developed by use of object-oriented methodology. The main function of the control system is to provide real-time heating information to technicians or automated machines. The information includes heating location, torch speed, heating order, and others. The system development is achieved by following the five steps in the object-oriented procedure. First, requirements are specified and corresponding objects are determined. Then, the analysis, design, and implementation of the proposed system are sequentially carried out. The system consists of six subsystems, or modules. These are (1) the inference module with an artificial neural network algorithm, (2) the analysis module with the Finite Element Method and kinematics analysis, (3) the data access module to store and retrieve the forming information, (4) the communication module, (5) the display module, and (6) the measurement module. The system is useful, irrespective of the heating sources, i.e. flame/gas, laser, or high frequency induction heating. A newly developed automated line heating machine is connected to the proposed system. Experiments and discussions follow.