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http://dx.doi.org/10.5352/JLS.2018.28.6.726

The Preventive Effect of Bacillus polyfermenticus KJS-2 and Angelica gigas Nakai Extract on Triton WR-1339-induced Hyperlipidemia  

Kim, Kang Min (Department of Pharmaceutical Science and Technology, Kyungsung University)
Kim, Bo Seul (Department of Pharmacy, Kyungsung University)
Kang, Jae Seon (Department of Pharmacy, Kyungsung University)
Publication Information
Journal of Life Science / v.28, no.6, 2018 , pp. 726-732 More about this Journal
Abstract
The purpose of this study was to investigate the effects of Bacillus polyfermenticus KJS-2 (B. polyfermenticus KJS-2) and Angelica gigas Nakai extracts (AGNE) on hyperlipidermia. The purity of the major decursin and decursinol angelate (D/DA) in the AGNE were analyzed at 78%. Increased concentrations of AGNE (0.1-20 mg/ml) showed a higher 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase inhibition activity. Endospore-forming B. polyfermenticus KJS-2 ($1{\times}10^9CFU/ml$) exhibited good bile tolerance (0.3 mm) on an agar plate. An animal study was carried out using different groups, including a normal control, positive control (atorvastatin), negative control (triton WR-1339), AGNE group, B. polyfermenticus KJS-2 group, AGNE + B. polyfermenticus KJS-2 group, and Atorvastatin + AGNE + B. polyfermenticus KJS-2 group to determine the effect of hyperlipidemia. There were no significant changes in body weight, kidney weight, or liver weight except for the liver weight of the triton WR-1339-treated group. Groups with AGNE and B. polyfermenticus KJS-2 had increased HDL-cholesterol and decreased total cholesterol and triglycerides. The liver histopathological results also showed that all AGNE and B. polyfermenticus KJS-2-treated groups contained lower fat accumulation in the liver tissues. The findings of this study verified that AGNE and Endospore-forming B. polyfermenticus KJS-2 combination materials have a hyperlipidemic effect.
Keywords
Angelica gigas Nakai; Bacillus polyfermenticus KJS-2; decursin; decursinol angelate; hyperlipidermia;
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1 Choi, S. W., Bai, D. H., Yu, J. H. and Shin, C. S. 2003. Characteristics of the squalene synthase inhibitors produced by a Streptomyces species isolated from soils. Can. J. Microbiol. 49, 663-668.   DOI
2 Cho, M. S. 2010. Production optimization of spore and macrolatin A from Bacillus polyfermenticus KJS-2. Master. dissertation, Inje university, Kimhae, Korea.
3 Choi, S. M., Park, M. H., Jung, T. S., Moon, K. H., Kim, K. M. and Kang, J. S. 2011. Characterization of Bacillus mojavensis KJS-3 for industrial applications. Arch. Pharm. Res. 34, 289-298.   DOI
4 Dashkevicz, M. P. and Feighner, S. D. 1989. Development of a differential medium for bile salt hydrolase-active Lactobacillus spp. Appl. Environ. Microbiol. 55, 11-16.
5 Getachew, T. 2016. A review on effects of probiotic supplementation in poultry performance and cholesterol levels of egg and meat. J. World Poult. Res. 6, 31-36.
6 Ha, T. H. 2013. Effects of ephedra herba ethyl-acetate fraction (EEAF) on hyperlipidmicmice. Ph. D. dissertation, Dong-eui University, Busan, Korea.
7 Jun, K. D., Kim, H. J., Lee, K. H., Paik, H. D. and Kang, J. S. 2002. Characterization of Bacillus polyfermenticus SCD as a probiotic. Kor. J. Microbiol. Biotechnol. 30, 359-366.
8 Kang, J. S., Ji, Y. H., Lee, S. H., Kim, B. S., Ha, S. M., Kim, D. H. and Hong, Y. G. 2006. Pharmaceutical composition for treating hyperlipidaemia. Korea patent 10-1658350.
9 Kang, J. S., Jun, K. D., Kim, W. S., Jo, W. S., Kwon, J. Y. and Moon, K. H. 2004. Antibacterial Activities of B. polyfermenticus SCD against pathogenic bacteria and effects on animals and humans. Yakhak Hoeji 48, 70-74.
10 Kim, D. H., Kang, K. R., Kim, H. W., Yoon, S. Y., Kim, C. G., Yamaguchi, T., Kyung, S. J. and Kang, J. S. 2010. Structure determination of macrolactin compounds with antibacterial activities isolated from Bacillus polyfermenticus KJS-2. J. Life Sci. 20, 1792-1800.   DOI
11 Kim, D. H., Kim, H. K., Kim, K. M., Kim, C. K., Jeong, M. H., Ko, C. Y., Moon, K. H. and Kang, J. S. 2011. Antibacterial activities of macrolactin A and 7-O-succinyl macrolactin A from Bacillus polyfermenticus KJS-2 against vancomycin-resistant enterococci and methicillin-resistant Staphylococcus aureus. Arch. Pharm. Res. 34, 147-152.   DOI
12 Kim, G. S., Park, C. G., Jeong, T. S., Cha, S. W., Baek, N. I. and Song, K. S. 2009. ACAT (Acyl-CoA:cholesterol Acyltransferase) inhibitory effect and quantification of pyranocurmarin in different parts of Angelica gigas Nakai. J. Appl. Biol. Chem. 52, 188-194.
13 Kim, K. M., Jung, J. Y., Hwang, S. W., Kim, M. J. and Kang, J. S. 2009. Isolation and purification of decursin and decursinol angelate in Angelica gigas Nakai. J. Kor. Soc. Food Sci. Nutr. 38, 653-656.   DOI
14 Kim, K. M., Kim, M. J., Kim, D. H., Park, Y. S. and Kang, J. S. 2009. Characterization of Bacillus polyfermenticus KJS-2 as a probiotic. J. Microbiol. Biotechnol. 19, 1013-1018.   DOI
15 Kim, K. M., Kim, T. H., Park, Y. J., Kim, I. H. and Kang, J. S. 2009. Evaluation of the genotoxicity of decursin and decursinol angelate produced by Angelica gigas Nakai. Mol. Cell. Toxicol. 5, 83-87.
16 Kim, K. M., Lee, Y. J., Hong, Y. G. and Kang, J. S. 2009. Oral acute and subacute toxicity studies of decursin and decursinol angelate of Angelica gigas Nakai. Mol. Cell. Toxicol. 5, 153-159.
17 Kim, K. M., Seo, J. L. and Kang, J. S. 2014. Decursin and decursinol angelate affect spermatogenesis in the adult rat at oral administration. Mol. Cell. Toxicol. 10, 83-89.   DOI
18 Kim, M. K., Kwon, B. M., Bae, K. H., Choi, D. H. and Lee, H. J. 1999. Screening of Acyl-CoA:cholesterol acyltransferase (ACAT) inhibitors from natural sources. Kor. J. Pharmacogn. 30, 384-396.
19 Kim, S. H., Ahn, B. C., Joung, H. J. and Park, M. J. 2012. Lipid profiles and prevalence of dyslipidemia in Korean adolescents. Endocrinol. Metab. 27, 208-216.   DOI
20 Kim, W. T. 2012. Effect of Angelica gigas Nakai extract on hepatic damage in rats. Master. dissertation, Kyung-sung university, Busan, Korea.
21 Korea Institute for Health & Social Affairs. Korean Statistical Information Service. The statistics of DT_33109_A206, http://kosis.kr/statHtml/statHtml.do?orgId=331&tblId=DT_33109_A206&conn_path=I3
22 Ok, S., Lee, J. H., Kim, I. H. and Kang, J. S. 2011. Glucose transporters and AMP-activated protein kinase modulation effects of decursin and decursinol angelate on diabetic rats. Yakhak Hoeji 55, 301-308.
23 Ooi, L. G. and Liong, M. T. 2010. Cholesterol-lowering effects of probiotics and prebiotics: A review of in vivo and in vitro findings. Int. J. Mol. Sci. 11, 2499-2522.   DOI
24 Park, H. Y. 2000. Hyperlipidemia. Kor. J. Med. 59, 596-599.
25 Park, I. S., Ahn, S. H. and Kim, J. T. 1997. Accumulation of lipid including cholesterol in murine hepatocytes with hyperlipidemia induced by Triton WR-1339. Dongguk J. The Institute of Oriental Medicine 6, 107-115.
26 Song, H. G. and Lee, H. 2011. The Effects of persicae semen herbal-acupuncture at GB34 (Yangneungcheon) on a rat model of hyperlipidemia. J. Kor. Med. 32, 151-163.
27 Tsai, C. C., Lin, P. P., Hsieh, Y. M., Zhang, Z. Y., Wu, H. C. and Huang, C. C. 2014. Cholesterol-lowering potentials of lactic acid bacteria based on bile-Salt hydrolase activity and effect of potent strains on cholesterol metabolism in vitro and in vivo. Scientific World Journal 2014, 690752.
28 Virginia, A., Rachmawati, H., Riani, C. and Retnoningrum, D. S. 2016. Study of HMG-CoA reductase inhibition activity of the hydrolyzed product of snakehead fish (Channa striata) skin collagen with 50 kDa collagenase from Bacillus licheniformis F11.4. Sci. Pharm. 84, 81-88.   DOI
29 Xie, L., Li, J., Xie, L. and Bourne, P. E. 2009. Drug discovery using chemical systems biology: identification of the protein-ligand binding network to explain the side effects of CETP inhibitors. PLoS Comput. Biol. 5, e1000387.   DOI
30 Yu, B. Y., Kim, Y. S. and Yoo, B. W. 2012. Management of dyslipidemia in primary care. Kor. J. Fam. Pract. 2, 98-103.