• 한국축산식품학회지
• /
• 제30권5호
• /
• pp.804-810
• /
• 2010

This study was performed to select protease-producing Bacillus sp. as a potential probiotic starter for fermented meat products. In order to isolate protease-producing bacterium from meju, measured the diameter of the clear zone on agar plate (TSA, 1% (w/v) skim milk) and analyzed for intracellular protease activity, then 10 Bacillus-like strains were isolated. Three Bacillus-like strains (P19, P27, and P33) among 10 strains were able to tolerate in acidic condition (TSB, pH 2.5, 2 h incubation). These 3 strains were showed antimicrobial activity against food-borne pathogenic bacteria. These vegetative cells of 3 strains were showed a survival rate of 0.04% to 0.08% under the artificial gastric acidic condition (TSB, pH 2.5 with 1% (w/v) pepsin), but spore-forming cells were 56.29% to 84.77%. Vegetative cells of 3 strains were the least bile-resistant, while spore-forming cells of 3 strains showed higher survival rate more than 76% under artificial bile condition (TSB, 0.1% (w/v) oxgall bile). In these strains, P27 strain was finally selected as a good probiotic strain. P27 strain was tentatively identified as Bacillus amyloliquefaciens by API CHB kit and 16S rDNA sequence analysis. The results of this study suggest that B. amyloliquefaciens P27 can be used as a potential probiotic starter for fermented meat product.

• Journal of Advanced Marine Engineering and Technology
• /
• 제37권8호
• /
• pp.893-904
• /
• 2013

NSGA-II와 함께 크리깅 메타모델기반 다목적최적설계 전략을 3차원 CFD 시뮬레이션을 통해 액셜 피스톤 펌프의 밸브 플레이트 형상을 최적화하는데 적용하였다. 펌프의 압력 변동을 저감하고 수력 효율을 최대화하기 위한 최적설계 과정은 두 단계, 즉 (1) 밸브 플레이트 상의 6개 형상 설계 변수를 선정하고 각 설계변수의 변화에 따른 CFD 해석을 수행하며, (2) CFD 데이터를 이용한 NSGA-II에 기반한 다목적최적설계 접근방식으로 최소 맥동 압력과 펌프 효율 설계에 대해 파레토 프론트를 평가하는 것으로 구성된다. 이들 결과로부터 최소 맥동 압력을 가지며 액셜 피스톤 펌프의 목표 효율에 도달하는 최적 절충해를 선택할 수 있었다.

• Journal of Advanced Marine Engineering and Technology
• /
• 제22권2호
• /
• pp.139-145
• /
• 1998

Filament winding method is widely used for composite fabrications using low viscosity liquid for-mation and processing asymmetrical structures of pressure vessel pipe rocket motor case etc. The filament winding method is affected by several parameters such as pot life of process time viscosi-ty of resin filament winding temperature and schedules curing condition and post curing condi-tion of resin. To develope high functional composite materials the rotation(5, 15, 20, 30rpm) of the winding machine was controlled by D.C motor. And the wiper to give proper tension was equipped between strand and resin bath. The resin is hooked by the design wiper. The adequate cure schedule was found by DSC. NOL ring test is carried out to investigate the basic physical properties such as design technology. The void contents in filament winding is generally higher than that of the prepreg laminated plate. These high contents of void can make a crack in resin in spite of low deformation. These problem was solved by giving tension in processing. To improve the characteristics of fiber volume fraction void contents resin/fiber bonding the winding speedc is changed under constant tension. It was found that resin impregnation was not different from in fiber contents void contents at the range of 0.5~1kg tension but it was found that resin was not impregnated at the above of 1.5kg tension. In burst test a pure PE liner was failed at a nozzle part under the $14kg/\textrm{cm}^2$ pressure but a pressure vessel of CNG was failed at a cylinder part under the $200kg/\textrm{cm}^2$ pressure.

• 한국의학물리학회지:의학물리
• /
• 제13권2호
• /
• pp.62-68
• /
• 2002

최근 설치된 선형가속기는 다엽 콜리메이터를 장착하고 있다. 기본적으로 다엽 콜리메이트의 사용은 기존의 차폐블럭을 대체하는 역할을 한다. 그러나 다엽 콜리메이터는 폭 1 cm의 콜리메이터가 각각 움직여서 방사선 조사 범위를 만들어 정교한 조사범위를 만들기 어려운 경우가 있을 수 있다. 따라서 조사야를 보다 정밀하게 만들기 위해 다엽 콜리메이터 아래 추가 차폐물을 사용하게 된다. 다엽 콜리메이터 아래 추가 차폐물을 사용할 경우 차폐물과 환자의 피부표면과의 거리가 짧아져 피부표면 선량이 증가하게 되며 최대 선량점(D$_{max}$)이 변할 수 있다. 이와 같은 변화는 조사야의 크기와 방사선의 에너지에 따라 영향을 받을 수 있다. 따라서 본 연구는 다엽 콜리메이터 아래 추가 차폐물을 사용할 때 조사야와 방사선에너지에 따라 표면선량과 최대 선량점의 변화를 측정하여 이들 값이 증가함을 확인하였고 다엽 콜리메이터 아래 추가 차폐물을 사용함으로써 증가한 표면선량은 전자 흡수판으로 2-3 mm 두께의 납판을 사용하여 효과적으로 감소시킬 수 있음을 확인하였다.

• 대한치과보철학회지
• /
• 제35권4호
• /
• pp.719-741
• /
• 1997

This study was undertaken to compare by Atomic Force Microscope the effects of various finishing and polishing instruments on surface roughness of filling and veneering composite resins. Seven composite resins were studied : Silux Plus (3M Dental Products, U.S.A.), Charisma (Heraeus Kulzer, Germany), Prisma THP (L.D.Caulk, Dentsply, U.S.A.), Photoclearfil (Kuraray, Japan), Cesead (Kuraray, Japan), Thermoresin LC (GC, Japan), Artglass (Heraeus Kulzer, Germany). Samples were placed and polymerized in holes (2mm thick and 8.5mm in diameter) machined in Teflon mold under glass plate, ensuring excess of material and moulded to shape with polyester matrix strip. Except control group (Polyester matrix strip), all experimental groups were finished and polishied under manufacturer's instructions. The finishing and polishing procedure were : carbide bur (E.T carbide set 4159, Komet, Germany), diamond bur (composite resin polishing bur set, GC, Japan), aluminum-oxide disc (Sof-Lex Pop-On, 3M Dental Products, U.S.A.), diamond-particle disc (Dia-Finish, Renfert Germany), white stone bur & rubber point( composite finishing kit, EDENTA, Swiss), respectively. Each specimens were evaluated for the surface roughness with Atomic Force Microscope (AutoProbe CP, Park Scientific Instruments, U.S.A.) under contact mode and constant height mode. The results as follows : 1. Except Thermoresin LC, all experimental composite resin groups showed more rougher than control group after finishing and polishing(p<0.1). 2. A surface as smooth as control group was obtained by $Al_{2}O_{3}$ disc all filling composite resin groups except Charisma and all veneering composite resin groups except Thermoresin LC(p<0.05). 3. In case of Thermoresin LC, there were no statistically significant differences before and after finishing and polishing(p>0.1). 4. Carbide bur, diamond bur showed rough surfaces in all composite resin groups, so these were inappropriate for the final polishing instruments.

• 한국운동역학회지
• /
• 제20권2호
• /
• pp.239-243
• /
• 2010

The putting stroke accounts for 40~50% of total stroke for a golf rounding and most golfers have difficulties on the puting. Studies for the putting stroke have been conducted by analyzing various factors such as kinematics, kinetics, psychologic and physiologic parameters. A lot of devices were developed to support the studies. However there was no appropriate method to measure the position of the ball quantitatively. In this study, we developed a new measurement system to measure and evaluate the putting result. The developed system uses a USB camera to take the 2-dimensional image of the surface including the hole cup at the center of the image and the ball. The position of the ball is extracted as a set of distance and angle in polar coordinate system. We evaluated the new system with an indoor set-up for putting experiments and the system provided accurate measurement results. The proposed system can be combined with the other measurement systems such as 3D motion capture system and force plate without any restriction.

• Journal of Microbiology and Biotechnology
• /
• 제27권2호
• /
• pp.342-349
• /
• 2017

Polylactic acid (PLA) has been highlighted as an alternative renewable polymer for the replacement of petroleum-based plastic materials, and is considered to be biodegradable. On the other hand, the biodegradation of PLA by terminal degraders, such as microorganisms, requires a lengthy period in the natural environment, and its mechanism is not completely understood. PLA biodegradation studies have been conducted using mainly undefined mixed cultures, but only a few bacterial strains have been isolated and examined. For further characterization of PLA biodegradation, in this study, the PLA-degrading bacteria from digester sludge were isolated and identified using a polymer film-based screening method. The enrichment of sludge on PLA granules was conducted with the serial transference of a subculture into fresh media for 40 days, and the attached biofilm was inoculated on a PLA film on an agar plate. 3D optical microscopy showed that the isolates physically degraded the PLA film due to bacterial degradation. 16S rRNA gene sequencing identified the microbial colonies to be Pseudomonas sp. MYK1 and Bacillus sp. MYK2. The two isolates exhibited significantly higher specific gas production rates from PLA biodegradation compared with that of the initial sludge inoculum.

• 한국지반공학회:학술대회논문집
• /
• 한국지반공학회 2008년도 추계 학술발표회
• /
• pp.1427-1439
• /
• 2008

The quality of track-bed fills of railways has been controlled by field measurements of density (${\gamma}_d$) and the results of plate-load tests. The control measures are compatible with the design procedures whose design parameter is $k_{30}$ for both ordinary-speed railways and high-speed railways. However, one of fatal flaws of the design procedures is that there are no simple laboratory measurement procedures for the design parameters ($k_{30}$ or, $E_{v2}$ and $E_{v2}/E_{v1}$) in design stage. A new quality control procedure, in parallel with the advent of the new design procedure, is being proposed. This procedure is based upon P-wave velocity involving consistently the evaluation of design parameters in design stage and the field measurements during construction. The key concept of the procedure is that the target value for field compaction control is the P-wave velocity determined at OMC using modified compaction test, and direct-arrival method is used for the field measurements during construction. The procedure was verified at a test site and the p-wave velocity turned out to be an excellent control measure. The specifications for the control also include field compaction water content of $OMC{\pm}2%$ as well as the p-wave velocity.

• 한국통신학회논문지
• /
• 제18권4호
• /
• pp.468-480
• /
• 1993

본 논문에서는 2개의 동일한 1-Port 형 공진기를 병렬로 근접배치시킨 이중모드 SAW 공진기의 설계를 위하여, 우선, 결합 모드이론에 의한 1-Port 형 공진기의 특성을 기초로 하여, 도파로 모델을 적용한 이중모드 SAW 공진기의 특성을 분석하였으며, 이를 이용해서 중심 주파수가 150.15MHz이고 대역폭이 80KHz인 2-Pole 및 4-Pole 협대역 필터를 설계.제작 하였다. 이중모드 SAW 필터를 설계변수를 달리하여 여러 번 제작.실험하여 실험치와 이론치를 비교함으로써, 제품 설계에 사용 가능한 실험적 설계 특성을 얻었으며, 이동통신기기에서 사용될 수 있는 헙대역 통과 필터를 구현하였다. DMS 공진기를 구성한 압전 기판으로는 높은 주파수에서도 온도변화에 의한 주파수 이동 및 물성의 변화가 적은 ST-cut quartz(수정)를 선택하였으며, 필터의 제작은 정확한 전극구조를 얻기 위하여 고해상도 사진 식각법과 전극의 수직식각특성이 우수한 이온반응 식각법(Reactive Ion Etching)을 적용하였다.

• 한국운동역학회지
• /
• 제24권2호
• /
• pp.95-101
• /
• 2014

The purpose of this study was to investigate the training and detraining effects of a 8-week water exercise on lower extremities coordination during obstacle gait in the female elderly. Eight elderly participants (age: $76.58{\pm}4.97$ yrs, height: $148.88{\pm}7.19$ cm, body mass: $56.62{\pm}6.82$ kg, and leg length: $82.36{\pm}2.98$ cm), who stayed at the Seoul K welfare center, were recruited for this study. All participants had no history of orthopedic abnormality within the past 1 year and completed the aquatic exercise program which lasted for 8 weeks. To identify the training and detraining effect of 8 weeks of water exercise, a 3-D motion analysis with 7 infrared cameras and one force plate sampling frequency set at 100 Hz and 1,000 Hz, respectively, was performed. A two-way ANOVA was performed to find training and detraining effects among diferent obstacle heights. In this study significant level was set at .05. Significant training effects of LTS (lead foot thigh and shank) coordination in all obstacle height were found (p<.05). It is also found that the training effect of LTS remained 37%, 58%, and 25% in obstacle height of 30%, 40%, and 50%, respectively. Lead foot showed the greater detraining effect of coordination compared with trail foot, and SF (shank and foot) coordination revealed better detraining effects of coordination compare with TS (thigh and shank) in both feet. Based on the findings, a 8 week water exercise give an positive effects to the elderly in terms of segment cooperation which potentially helps reducing their accident falls. The magnitude of detraining may also help the elderly to find the retraining moment.