• Title/Summary/Keyword: 2D plate

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Isolation and Characterization of a Protease-Producing Bacterium, Bacillus amyloliquefaciens P27 from Meju as a Probiotic Starter for Fermented Meat Products

  • Lee, Mi-Sun;Lee, Na-Kyoung;Chang, Kyung-Hoon;Choi, Shin-Yang;Song, Chi-Kwang;Paik, Hyun-Dong
    • Food Science of Animal Resources
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    • v.30 no.5
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    • pp.804-810
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    • 2010
  • This study was performed to select protease-producing Bacillus sp. as a potential probiotic starter for fermented meat products. In order to isolate protease-producing bacterium from meju, measured the diameter of the clear zone on agar plate (TSA, 1% (w/v) skim milk) and analyzed for intracellular protease activity, then 10 Bacillus-like strains were isolated. Three Bacillus-like strains (P19, P27, and P33) among 10 strains were able to tolerate in acidic condition (TSB, pH 2.5, 2 h incubation). These 3 strains were showed antimicrobial activity against food-borne pathogenic bacteria. These vegetative cells of 3 strains were showed a survival rate of 0.04% to 0.08% under the artificial gastric acidic condition (TSB, pH 2.5 with 1% (w/v) pepsin), but spore-forming cells were 56.29% to 84.77%. Vegetative cells of 3 strains were the least bile-resistant, while spore-forming cells of 3 strains showed higher survival rate more than 76% under artificial bile condition (TSB, 0.1% (w/v) oxgall bile). In these strains, P27 strain was finally selected as a good probiotic strain. P27 strain was tentatively identified as Bacillus amyloliquefaciens by API CHB kit and 16S rDNA sequence analysis. The results of this study suggest that B. amyloliquefaciens P27 can be used as a potential probiotic starter for fermented meat product.

Multiobjective optimization strategy based on kriging metamodel and its application to design of axial piston pumps (크리깅 메타모델에 기반한 다목적최적설계 전략과 액셜 피스톤 펌프 설계에의 응용)

  • Jeong, Jong Hyun;Baek, Seok Heum;Suh, Yong Kweon
    • Journal of Advanced Marine Engineering and Technology
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    • v.37 no.8
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    • pp.893-904
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    • 2013
  • In this paper, a Kriging metamodel-based multi-objective optimization strategy in conjunction with an NSGA-II(non-dominated sorted genetic algorithm-II) has been employed to optimize the valve-plate shape of the axial piston pump utilizing 3D CFD simulations. The optimization process for minimum pressure ripple and maximum pump efficiency is composed of two steps; (1) CFD simulation of the piston pump operation with various combination of six parameters selected based on the optimization principle, and (2) applying a multi-objective optimization approach based on the NSGA-II using the CFD data set to evaluate the Pareto front. Our exploration shows that we can choose an optimal trade-off solution combination to reach a target efficiency of the axial piston pump with minimum pressure ripple.

A Study on the Preocessing of high Runctional Composites and the Evaluation of Its Characteristics (고기능성 복합재료의 제조와 그 특성평가에 관한 연구)

  • 김윤해
    • Journal of Advanced Marine Engineering and Technology
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    • v.22 no.2
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    • pp.139-145
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    • 1998
  • Filament winding method is widely used for composite fabrications using low viscosity liquid for-mation and processing asymmetrical structures of pressure vessel pipe rocket motor case etc. The filament winding method is affected by several parameters such as pot life of process time viscosi-ty of resin filament winding temperature and schedules curing condition and post curing condi-tion of resin. To develope high functional composite materials the rotation(5, 15, 20, 30rpm) of the winding machine was controlled by D.C motor. And the wiper to give proper tension was equipped between strand and resin bath. The resin is hooked by the design wiper. The adequate cure schedule was found by DSC. NOL ring test is carried out to investigate the basic physical properties such as design technology. The void contents in filament winding is generally higher than that of the prepreg laminated plate. These high contents of void can make a crack in resin in spite of low deformation. These problem was solved by giving tension in processing. To improve the characteristics of fiber volume fraction void contents resin/fiber bonding the winding speedc is changed under constant tension. It was found that resin impregnation was not different from in fiber contents void contents at the range of 0.5~1kg tension but it was found that resin was not impregnated at the above of 1.5kg tension. In burst test a pure PE liner was failed at a nozzle part under the $14kg/\textrm{cm}^2$ pressure but a pressure vessel of CNG was failed at a cylinder part under the $200kg/\textrm{cm}^2$ pressure.

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Surface and Percentage Depth Doses for Multileaf Collimator Conjunction with Conventional Block (다엽 콜리메이터와 제작차폐물의 동시 사용시 표면선량 변화)

  • 양광모;서현숙
    • Progress in Medical Physics
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    • v.13 no.2
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    • pp.62-68
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    • 2002
  • A muiltileaf collimator (MLC) is used as a replacement for conventional blocks. The MLC, however may not be appropriate for a fine field shaping. For the fine field shaping, conventional block can be added under the MLC. But it may significantly affect on the dosimetric characteristics such as surface dose of skin, buildup region and percent depth doses. We performed the study to evaluate the surface dose and the maximum depth dose using MLC conjunction with conventional blocks for various field sizes and energies. We confirmed the surface dose was increased by using the additional conventional block under the MLC ranging from 10 to 35.6% according to various field sizes and radiation beam energies. The surface dose was effectively reduced by application of 2 or 3 m thickness of lead plate as electron filter.

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A STUDY ON SURFACE ROUGHNESS OF COMPOSITE RESINS AFTER FINISHING AND POLISHING -an Atomic Force Microscope study (연마방법에 따른 복합레진의 활택도에 관한 연군 -Atomic Force Microscope를 이용한 연구)

  • Kim, Hyeong-Seob;Woo, Yi-Hyung
    • The Journal of Korean Academy of Prosthodontics
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    • v.35 no.4
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    • pp.719-741
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    • 1997
  • This study was undertaken to compare by Atomic Force Microscope the effects of various finishing and polishing instruments on surface roughness of filling and veneering composite resins. Seven composite resins were studied : Silux Plus (3M Dental Products, U.S.A.), Charisma (Heraeus Kulzer, Germany), Prisma THP (L.D.Caulk, Dentsply, U.S.A.), Photoclearfil (Kuraray, Japan), Cesead (Kuraray, Japan), Thermoresin LC (GC, Japan), Artglass (Heraeus Kulzer, Germany). Samples were placed and polymerized in holes (2mm thick and 8.5mm in diameter) machined in Teflon mold under glass plate, ensuring excess of material and moulded to shape with polyester matrix strip. Except control group (Polyester matrix strip), all experimental groups were finished and polishied under manufacturer's instructions. The finishing and polishing procedure were : carbide bur (E.T carbide set 4159, Komet, Germany), diamond bur (composite resin polishing bur set, GC, Japan), aluminum-oxide disc (Sof-Lex Pop-On, 3M Dental Products, U.S.A.), diamond-particle disc (Dia-Finish, Renfert Germany), white stone bur & rubber point( composite finishing kit, EDENTA, Swiss), respectively. Each specimens were evaluated for the surface roughness with Atomic Force Microscope (AutoProbe CP, Park Scientific Instruments, U.S.A.) under contact mode and constant height mode. The results as follows : 1. Except Thermoresin LC, all experimental composite resin groups showed more rougher than control group after finishing and polishing(p<0.1). 2. A surface as smooth as control group was obtained by $Al_{2}O_{3}$ disc all filling composite resin groups except Charisma and all veneering composite resin groups except Thermoresin LC(p<0.05). 3. In case of Thermoresin LC, there were no statistically significant differences before and after finishing and polishing(p>0.1). 4. Carbide bur, diamond bur showed rough surfaces in all composite resin groups, so these were inappropriate for the final polishing instruments.

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Development of a Golf Putting Result Recording System Using USB Camera (USB 카메라를 이용한 골프 퍼팅 결과 기록 장치의 개발)

  • Kim, Hyung-Sik;Choi, Jin-Seung;Tack, Gye-Rae;Lim, Young-Tae;Yi, Jeong-Han
    • Korean Journal of Applied Biomechanics
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    • v.20 no.2
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    • pp.239-243
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    • 2010
  • The putting stroke accounts for 40~50% of total stroke for a golf rounding and most golfers have difficulties on the puting. Studies for the putting stroke have been conducted by analyzing various factors such as kinematics, kinetics, psychologic and physiologic parameters. A lot of devices were developed to support the studies. However there was no appropriate method to measure the position of the ball quantitatively. In this study, we developed a new measurement system to measure and evaluate the putting result. The developed system uses a USB camera to take the 2-dimensional image of the surface including the hole cup at the center of the image and the ball. The position of the ball is extracted as a set of distance and angle in polar coordinate system. We evaluated the new system with an indoor set-up for putting experiments and the system provided accurate measurement results. The proposed system can be combined with the other measurement systems such as 3D motion capture system and force plate without any restriction.

Polymer Film-Based Screening and Isolation of Polylactic Acid (PLA)-Degrading Microorganisms

  • Kim, Mi Yeon;Kim, Changman;Moon, Jungheun;Heo, Jinhee;Jung, Sokhee P.;Kim, Jung Rae
    • Journal of Microbiology and Biotechnology
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    • v.27 no.2
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    • pp.342-349
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    • 2017
  • Polylactic acid (PLA) has been highlighted as an alternative renewable polymer for the replacement of petroleum-based plastic materials, and is considered to be biodegradable. On the other hand, the biodegradation of PLA by terminal degraders, such as microorganisms, requires a lengthy period in the natural environment, and its mechanism is not completely understood. PLA biodegradation studies have been conducted using mainly undefined mixed cultures, but only a few bacterial strains have been isolated and examined. For further characterization of PLA biodegradation, in this study, the PLA-degrading bacteria from digester sludge were isolated and identified using a polymer film-based screening method. The enrichment of sludge on PLA granules was conducted with the serial transference of a subculture into fresh media for 40 days, and the attached biofilm was inoculated on a PLA film on an agar plate. 3D optical microscopy showed that the isolates physically degraded the PLA film due to bacterial degradation. 16S rRNA gene sequencing identified the microbial colonies to be Pseudomonas sp. MYK1 and Bacillus sp. MYK2. The two isolates exhibited significantly higher specific gas production rates from PLA biodegradation compared with that of the initial sludge inoculum.

A Compaction Control Procedure of Railway Trackbed Fills Using Elastic Waves (탄성파를 이용한 철도노반의 다짐관리 방안)

  • Park, Chul-Soo;Park, In-Beom;Oh, Sang-Hoon;Kim, Hak-Sung;Mok, Young-Jin
    • Proceedings of the Korean Geotechical Society Conference
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    • 2008.10a
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    • pp.1427-1439
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    • 2008
  • The quality of track-bed fills of railways has been controlled by field measurements of density (${\gamma}_d$) and the results of plate-load tests. The control measures are compatible with the design procedures whose design parameter is $k_{30}$ for both ordinary-speed railways and high-speed railways. However, one of fatal flaws of the design procedures is that there are no simple laboratory measurement procedures for the design parameters ($k_{30}$ or, $E_{v2}$ and $E_{v2}/E_{v1}$) in design stage. A new quality control procedure, in parallel with the advent of the new design procedure, is being proposed. This procedure is based upon P-wave velocity involving consistently the evaluation of design parameters in design stage and the field measurements during construction. The key concept of the procedure is that the target value for field compaction control is the P-wave velocity determined at OMC using modified compaction test, and direct-arrival method is used for the field measurements during construction. The procedure was verified at a test site and the p-wave velocity turned out to be an excellent control measure. The specifications for the control also include field compaction water content of $OMC{\pm}2%$ as well as the p-wave velocity.

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Double-Mode SAW Filter for Mobile Communication System (이중 모드 결합에 의한 이동 통신 기기용 SAW 필터)

  • 정영지;진익수;황금찬
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.18 no.4
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    • pp.468-480
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    • 1993
  • In this paper, at first, the characteristics of double mode SAW (DMS) resonator are analysed by applying the wave guide model and coupled mode theory to 1-port resonator. The DMS resonators (2-pole), which are arranged in a close, parallel configuration of two identical 1-port resonators on a single plate, and 4-pole DMS filters are designed and fabricated at the center frequency of 150.15 MHz with 3-dB bandwidth of 80 KHz. The empirical design characteristics are obtained from the comparison of experimental and theoretical values resulted from several fabrications, and the narrow bandpass filters are implemented on the basis of the above empirical results, which can be used to mobile communication systems. A ST-cut quartz substrate is selected for the stable temperature-frequence characteristics, and high resolution photolithography is applied to the fabrication of filter to get the fine electrode patterns.

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Effects of a Water Exercise on the Lower Extremities Coordination during Obstacle Gait in the Female Elderly - Focusing on Training and Detraining Effects - (수중운동이 여성노인 장애물보행 시 하지 협응에 미치는 영향 - 훈련 및 훈련잔여효과 중심으로 -)

  • Yoon, Sukhoon;Chang, Jae-Kwan;Kim, Joonyeon
    • Korean Journal of Applied Biomechanics
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    • v.24 no.2
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    • pp.95-101
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    • 2014
  • The purpose of this study was to investigate the training and detraining effects of a 8-week water exercise on lower extremities coordination during obstacle gait in the female elderly. Eight elderly participants (age: $76.58{\pm}4.97$ yrs, height: $148.88{\pm}7.19$ cm, body mass: $56.62{\pm}6.82$ kg, and leg length: $82.36{\pm}2.98$ cm), who stayed at the Seoul K welfare center, were recruited for this study. All participants had no history of orthopedic abnormality within the past 1 year and completed the aquatic exercise program which lasted for 8 weeks. To identify the training and detraining effect of 8 weeks of water exercise, a 3-D motion analysis with 7 infrared cameras and one force plate sampling frequency set at 100 Hz and 1,000 Hz, respectively, was performed. A two-way ANOVA was performed to find training and detraining effects among diferent obstacle heights. In this study significant level was set at .05. Significant training effects of LTS (lead foot thigh and shank) coordination in all obstacle height were found (p<.05). It is also found that the training effect of LTS remained 37%, 58%, and 25% in obstacle height of 30%, 40%, and 50%, respectively. Lead foot showed the greater detraining effect of coordination compared with trail foot, and SF (shank and foot) coordination revealed better detraining effects of coordination compare with TS (thigh and shank) in both feet. Based on the findings, a 8 week water exercise give an positive effects to the elderly in terms of segment cooperation which potentially helps reducing their accident falls. The magnitude of detraining may also help the elderly to find the retraining moment.