• Journal of Microbiology and Biotechnology
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• 제11권2호
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• pp.173-178
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• 2001

A monoclonal antibody (mAb) to the glucoprotein D (gD) of Herpes simplex virus type 2 (HSV-2) was successfully generated by hybridoma technology and characterized. The mAb, SKS2v, recognized a gD antigen with an apparent molecular mass of 60kDa in a Western blot analysis. The isotype was determined by a sandwich ELISA to be IgG2a. HSV-2 exhibited major antigens of 36, 43, 46, 47, 60, 69, 81, 96, 109, 112, 159, and 227 kDa among 25 protein profiles in SDS-PAGE, and among these antigens, those of 60, 112, 125, and 227 kDa were immunodominant in a Western blot analysis using antisera, thereby indicating that they play a role in inducing neutralizing antibodies in HSV-2 infection. When reacted with Vero cells infected with HSV-1 and HSV-2 SKSv2 showed a reactivity to the surface of the infected cells, and a gD antigen of 60 kDa appeared to be expressed in both types of HSV.

• 대한전기학회논문지:전기기기및에너지변환시스템부문B
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• 제49권2호
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• pp.93-101
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• 2000

This paper presents a novel method for the transient of eddy currents in the dampers of a super-conducting synchronous generator(SCG). The method proposes a 2-D corrected model which takes into account the influence of leakage fluxes of the field winding ends by increasing the effective air gap in order to consider the high precision of the analysis for the conventional 2-D model. The electromagnetic fields for the corrected model are analyzed by the time-stepping finite element method, thus the eddy currents in the dampers and electro-motive forces(EMF) in the stator windings are calculated. As the results, it is proved the presented method is comparatively accurate by comparing measured phase EMF values and the simulation ones, where about 6.4% error at the maximum value of EMF is occurred between them.

• 한국방재학회 논문집
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• 제8권2호
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• pp.119-127
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• 2008

본 연구에서는 하천의 지형이 복잡하고 지류 합류부 및 여러 수리구조물이 위치한 공주대교 상 하류구간에 대하여 흐름특성과 하상변동 양상을 분석하였다. 대상구간의 흐름특성 모의를 위해 1차원 HEC-RAS와 2차원 RMA2를 적용하였으며, 모의 결과 대상구간의 종방향 수위는 1차원과 2차원 모형의 결과가 양호하게 일치하였다. 그리고 하상변동 예측을 위해 2차원 SED2D를 적용하였으며, 모의 결과 단면의 급변 구간과 교량구간, 지류 합류구간, 만곡부 구간 등 주요 단면에서 횡방향 하상변동의 정량적인 분석이 가능하였다. 또한, 주요 단면에서의 하상변동 분포는 유속과 밀접한 관계를 가지는 것으로 나타났다. SED2D 모형의 민감도 분석을 실시한 결과 부유사 농도, 모래층의 두께, 이송가능한 모래크기는 하상변동에 민감한 영향을 미치는 것으로 나타났으며, 이러한 결과는 향후 하상변동 모형을 적용함에 있어 활용될 수 있을 것으로 사료된다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2005년도 제36회 하계학술대회 논문집 B
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• pp.987-989
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• 2005

Axi-periodic analysis using magnetic vector potential is formulated in time harmonic field and applied to the field analysis for the end region of large turbo generator in this paper. By using axi-periodic analysis, the effect of flux shield, one of the structure placed in the end region of large turbo generator to prevent stator end from thermal damage, is studied, and eddy current loss in the flux shield is estimated for operation conditions. 3D FEA is used for the verification of presented analysis method. Because 3D flux distribution can be calculated with 2D modeling, magnetic field showing 3D distribution can be effectively calculated by axi-periodic analysis comparing with 3D FEA.

• 지구물리와물리탐사
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• 제7권1호
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• pp.7-13
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• 2004

In this paper, we demonstrate that Common Mid-Point (CMP) cross-correlation gathers of multi-channel and multi-shot surface waves give accurate phase-velocity curves, and enable us to reconstruct two-dimensional (2D) velocity structures with high resolution. Data acquisition for CMP cross-correlation analysis is similar to acquisition for a 2D seismic reflection survey. Data processing seems similar to Common Depth-Point (CDP) analysis of 2D seismic reflection survey data, but differs in that the cross-correlation of the original waveform is calculated before making CMP gathers. Data processing in CMP cross-correlation analysis consists of the following four steps: First, cross-correlations are calculated for every pair of traces in each shot gather. Second, correlation traces having a common mid-point are gathered, and those traces that have equal spacing are stacked in the time domain. The resultant cross-correlation gathers resemble shot gathers and are referred to as CMP cross-correlation gathers. Third, a multi-channel analysis is applied to the CMP cross-correlation gathers for calculating phase velocities of surface waves. Finally, a 2D S-wave velocity profile is reconstructed through non-linear least squares inversion. Analyses of waveform data from numerical modelling and field observations indicate that the new method could greatly improve the accuracy and resolution of subsurface S-velocity structure, compared with conventional surface-wave methods.

• Steel and Composite Structures
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• 제35권2호
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• pp.199-213
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• 2020

This paper proposes a Global-Local Analysis Method (GLAM) to assess the progressive collapse of steel framed structures under fire-induced column failure. GLAM obtains the overall structural response by combining dynamic analysis of the heated column (local) with static analysis of the overall structure (global). Test results of two steel frames which explicitly consider the dynamic effect during fire-induced column failure were employed to validate the proposed GLAM. Results show that GLAM gives reasonable predictions to the test frames in terms of both whether to collapse and the displacement verse temperature curves. Besides, several case studies of a two-dimensional (2D) steel frame and a three-dimensional (3D) steel frame with concrete slabs were conducted by using GLAM. Results show that GLAM gives the same collapse predictions to the studied cases with nonlinear dynamic analysis of the whole structure model. Compared with nonlinear dynamic analysis of the whole structure model, GLAM saves approximately 70% and 99% CPU time for the cases of 2D and 3D steel frame, respectively. Results also show that the load level of a structure has notable effects on the restraint condition of a heated column in the structure.

• International Journal of Industrial Entomology and Biomaterials
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• 제3권2호
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• pp.121-126
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• 2001

A cDNA encoding a cathepsin D homologue was cloned from a cDNA library of the mulberry longicorn beetle, Apriona germari. Sequence analysis of the cDNA encoding the cathepsin D homologue of A. germari revealed that the 1,158 bp cDNA has an open reading frame of 386 amino acid residues. The deduced protein sequence of the A. germari cathepsin D homologue shows high homology with cathepsin D in insects, Aedes aegypti (68.2% amino acid similarity) and Drosophila melanogaster (67.2% amino acid similarity). Two aspartic residues and six cystein residues in the A. germari cathepsin D homologue are present at identical locations in all of the other catepsins D. Unlike cathepsins D in two insect species, A. gemari cathepsin D homologue appears to have two putative glycosylation sites, rather than one. Phylogenetic analysis revealed the A. germari cathepsin D homologue is more closely related to insect cathepsins D than to the other animal cathepsins D. Northern blot analysis suggests that A. germari cathepsin D homologue gene is expressed in most if not all, body tissues.

• 한국지반공학회지:지반
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• 제14권5호
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• pp.5-16
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• 1998

공사중 터널은 막장부의 종방향 및 횡방향 아치거동에 의하여 주변 지반은 3차원적 변형거동을 보이나, 터널 안정성 해석에는 전산효율 등의 이유로 2차원 수치해석법이 일반적으로 적용되고 있다. 하중분배율은 터널의 굴진효과를 고려하기 위하여 2차원 해석에 도입된 것으로 지반변위, 숏크 리트 및 록볼트와 같은 1차 지보재의 하중 등과 같은 해석결과에 큰 영향을 미친다. 또한, 3차원 해석을 이용한 기존의 연구에 의하면, 지반의 변형특성, 터널크기, 굴진장 등이 하중분배율에 주요한 영향을 미친다는 사실이 입증되었다. 그러나, 설계조건에 대한 하중분배율의 정량적 산정법의 부재 로 인하여 실제 해석시에는 설계조건을 무시한 하중분배율이 적용되고 있는 실정이다. 이에, 본 논문에서는 기존 연구(정 대열, 1993)에서 제시한 72개의 3차원 해석결과에 대한 회귀분석을 통하여 하중분배율을 정량적으로 산정할 수 있는 방법을 제시하였다. 또한. ring-cut공법은 막장의 자립성 이 매우 불량한 조건에 적용되는 공법임에도 불구하고 기존의 2차원 해석 법으로는 막장코아의 진 지효과를 고려하지 못하는 문제점이 있었다. 따라서, 2차원 및 3차원 수치해석을 통하여 ring-cut공 법에 대한 기존 해석법의 문제점을 검토하였으며 본 공법에 대한 하중분배율의 보정치를 제시하였 다.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2000년도 봄 학술발표회 논문집
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• pp.467-474
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• 2000

The application of Terzaghi's theory of consolidation for analysing the settlement of multi-layered soils is not strictly valid because the theory involves an assumption that the soil is homogeneous. The settlement of stratified soils with confined aquifer can be analysed using numerical techniques whereby the governing differential equation is replaced by 2-dimensional finite difference approximations. The problems of discontinuous layer interface are very important in the algorithm and programming for the analysis of multi-layered consolidation using a numerical analysis, finite difference method(F.D.M.). Better results can be obtained by the process for discontinuous layer interface, since it can help consolidation analysis to model the actual ground The purpose of this paper provides an efficient computer algorithm based on numerical analysis using finite difference method(F.D.M) which account for multi-layered soils with confined aquifer to determine the degree of consolidation and excess pore pressures relative to time and positions more realistically.

• 경영과학
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• 제34권2호
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• pp.67-83
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• 2017

This paper proposes a R&D project performance measurement model for private firms combining balanced scorecard (BSC) and data envelopment analysis (DEA). The efficiency of R&D projects is measured in terms of each of the three perspectives of BSC by using DEA : the internal process perspective (DEA-P), the customer perspective (DEA-C), and the financial perspective (DEA-F). The performance indicators of the three perspectives of BSC are considered as outputs of the corresponding DEA models. To provide strategic implications for R&D planning, we also propose the R&D project performance matrices composed of two different types of efficiency dimensions. The proposed model is expected to be fruitfully utilized for R&D performance measurement of private firms.