• Title/Summary/Keyword: 2D, 3D의 공존

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Rec-assay and DNA-breaking Action on the Engymatic Browning Reaction Products -Influence of Cupric Ion Concentration- (효소적(酵素的) 갈변반응(褐變反應) 생성물(生咸物)에 대(對)한 Rec-assay 및 DNA 절단(切斷) 작용(作用) -동(銅) Ion 농도(濃度)의 영향(影響)-)

  • Ham, S.S.;Park, B.K.;Lee, S.Y.;Lee, J.H.;Kang, C.K.;Lee, D.S.;Omura, Hirohisa
    • Applied Biological Chemistry
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    • v.27 no.4
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    • pp.264-270
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    • 1984
  • In order to obtain mutagenic data of enzymatic browning reaction products, we investigated their mutagenicity. The rec-assay with Bacillus subtilis strains $H17(rec^+)$ and $M45(rec^-)$ were carried out with their spores. Detection of double strand breakage in calf-thymus DNA was investigated into sample solution with and without $Cu^{2+}$ by the agarose slab gel electrophoresis. In the rec-assay, catechol, pyrocatechol, DL-dopa, 3,4-dihydroxytoluene, hydroquinone, hydroxyhydroquinone with and without $Cu^{2+}$ in 0.05M ana 0.1M at the enzymatic browning reaction products stowed mutagenic action. And also browning solution of 0.05M hydroxyhydroquinone and catechol with $Cu^{2+}$, hydroquinone with and without $Cu^{2+}$ of 0.1M at the enzymatic browning reaction products were strong mutagenic action. The DNA breakability of the enzymatic browning reaction products of 0.1M pyrogallol was stronger than that of 0.05M pyrogallol browning solution with $Cu^{2+}$ and 3,4-dihydroxytoluene browning solution was stronger than that of 0.01M 3,4-dihydroxytoluene browning solution.

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Anaerobic Ammonium Oxidation(ANAMMOX) in a Granular Sludge Reactor and its Bio-molecular Characterization (입상 슬러지 반응조 내의 혐기성 암모늄 산화(ANAMMOX) 및 분자생태학적 특성 평가)

  • Han, Ji-Sun;Park, Hyun-A;Sung, Eun-Hae;Kim, Chang-Gyun;Yoon, Cho-Hee;Bae, Young-Shin
    • Journal of Korean Society of Environmental Engineers
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    • v.28 no.11
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    • pp.1213-1221
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    • 2006
  • In this study, granular sludge used in an anaerobic process treating brewery waste was inoculated in a laboratory scale of reactor to induce anaerobic ammonium oxidation(ANAMMOX). The reactor was operated with synthetic wastewater, which prepared at 1:1 ratio of $NH_4^+-N$ over $NO_2^--N$. Changes in nitrogen concentration, COD, alkalinity and gas production were analyzed. There are 3 phases of spanning in experimental period according to influent nitrogen concentration. In the Phase 1, each of the concentration of $NH_4^+-N$ and $NO_2^--N$ were increased from 1.91 $gN/m^3{\cdot}d$ to 14.29 $gN/m^3{\cdot}d$. Ammonium nitrogen loading(same as nitrite nitrogen) was 23.81 $gN/m^3{\cdot}d$ in the Phase 2 and 19.05 $gN/m^3{\cdot}d$ in the Phase 3, respectively $NO_2^--N$ has been removed up to 99% during whole period while the removal efficiency of $NH_4^+-N$ was significantly varied. In Phase 2, $NH_4^+-N$ was removed up to 75%. Microorganisms varied temporally through three phases were characterized by 16s rDNA analysis methods. ANAMMOX bacteria were dominantly found in phase 2 when the removal rate of $NO_2^--N$and $NH_4^+-N$ was the highest up to 99% and 75%, respectively. Due to erroneous exposed to air, the removal efficiency of $NH_4^+-N$ was unexpectedly lowered, but ANAMMOX bacteria still existed.

Establishment of Management Practices in Korean Turfgrass (Zoysia japonica Steud.) - 1. Survey of Major Weed Species Occurring in Korean Turfgrass and Their Control Methods (한국(韓國) 야생(野生) 잔디의 재배기술확립(栽培技術確立)을 위한 연구(硏究) - 제(第) 1 보(報) 주요잡초(主要雜草) 조사(調査) 및 방제법(方除法) 구명(究明))

  • Kim, Kil-Ung;Kim, Dal-Ung
    • Korean Journal of Weed Science
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    • v.1 no.1
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    • pp.78-83
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    • 1981
  • This study was attempted to determine the major weed species occurring in Korean turfgrass, Zoysia japonica Steud. and to evaluate the most effective means for controlling them. More or less 40 weed species were determined to occur in Korean turfgrass. In terms of quantity and frequency of occurrence, Trifolium repens L., Digitaria sanguinalis (L.) Scop., Draba nemorosa var. hebecarpa Lindbl, Erigeron canadensis L., and Cyperus amuricus Max were observed to be the major weeds. Simpson's index, 0.306 was obtained to be the major weeds. Simpson's index, 0.306 was obtained in the golf course, Jinryang, Gyungsan-gun, meaning that no specific weed species were dominant, but in Kyungpook National University campus, Trifolium repense L. was a single dominant species, indicating Simpson's index, 0.776. The most effective herbicides to control Trifolium repense L. and other broad leaf weeds were MCPP [2-(2-methyl-4-chlorophenoxy) propionic acid), followed by 2,4-D (2,4-dichlolophenoxy acetic acid). Increased rates of both herbicides increased markedly their effectivity against weeds, with very slight injury against turfgrass. MCPP at 2.8 kg(a.i.)/ha gave excellent control without any injury and 2,4-D at 1.0 kg(a.i.)/ha.

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Performance Comparison and Analysis between Keypoints Extraction Algorithms using Drone Images (드론 영상을 이용한 특징점 추출 알고리즘 간의 성능 비교)

  • Lee, Chung Ho;Kim, Eui Myoung
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.40 no.2
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    • pp.79-89
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    • 2022
  • Images taken using drones have been applied to fields that require rapid decision-making as they can quickly construct high-quality 3D spatial information for small regions. To construct spatial information based on drone images, it is necessary to determine the relationship between images by extracting keypoints between adjacent drone images and performing image matching. Therefore, in this study, three study regions photographed using a drone were selected: a region where parking lots and a lake coexisted, a downtown region with buildings, and a field region of natural terrain, and the performance of AKAZE (Accelerated-KAZE), BRISK (Binary Robust Invariant Scalable Keypoints), KAZE, ORB (Oriented FAST and Rotated BRIEF), SIFT (Scale Invariant Feature Transform), and SURF (Speeded Up Robust Features) algorithms were analyzed. The performance of the keypoints extraction algorithms was compared with the distribution of extracted keypoints, distribution of matched points, processing time, and matching accuracy. In the region where the parking lot and lake coexist, the processing speed of the BRISK algorithm was fast, and the SURF algorithm showed excellent performance in the distribution of keypoints and matched points and matching accuracy. In the downtown region with buildings, the processing speed of the AKAZE algorithm was fast and the SURF algorithm showed excellent performance in the distribution of keypoints and matched points and matching accuracy. In the field region of natural terrain, the keypoints and matched points of the SURF algorithm were evenly distributed throughout the image taken by drone, but the AKAZE algorithm showed the highest matching accuracy and processing speed.

Four-year Survey on Transitions of the Life Form of Plants after Developing Human-made Wetlands along Boknaecheon of Juam Lake (주암호 복내천 인공습지 조성 후 식물의 생활형에 대한 4년간의 변화 연구)

  • Kim, Chang-Hwan;Myung, Hyun
    • Korean Journal of Environment and Ecology
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    • v.23 no.1
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    • pp.30-40
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    • 2009
  • Employing the Numata-type life form, the paper analyzed changes of plants for 4 years at the Human-made Wetlands along Boknaecheon of Juam Lake since its creation. The number of the species planted at the Human-made Wetlands along Boknaecheon of Juam Lake, which was completed in December 2002, were 15 in total including, 10 families, 13 genera, 12 species and 3 varieties. As for the three-featured life forms of the planted species, there were 6 perennial hydatophytes, recording the biggest number of species in dormancy form; species each of $R_5$, $R_3$, $R_{2-3}$ respectively in radicoid from; 20 species of geomantic dissenminule form ($D_1$) in disseminule form and erecred type(e) existed the most in growth form. With regard to the 3 features of life form identified during the final year of the monitoring that lasted 5 years after the completion of the Wetlands, the number of species and individuals was found to have increased but there was no significant change of tendency as against the composition ration(%) of life form. There were 43 species of therophytes (Th) that covered 24.29% in dormancy form, while $R_5$ was prevalent in radicoid form and $D_4$, $D_1$, and $D_{1,4}$ comprosed 77.39% of the whole disseminule form. Growth form was surveyed in the order of erected type (e), bunch type (t), temporal rosette type (pr), branch type (b) and straight rosette type (ps) and these species comprised 64.97% of the whole flora. Consequently, in case of the artificial wetlands along the Boknaecheon of Juam Lake, it turned out that the vegetation type in which pioneer species of succession, or gradually stabilized perennial vegetation favoring Wetlands because the higher dormancy form has its perennial plants' composition ratio getting, the more its succession is progressing. Even though single grained plants ($R_5$) belonging to radicoid in breeding form, succession is predicted to take place considering the fact that they actually belong to ~ plants like Phragmites japonica that form a connection on the surface of the earth. In addition, it is judged that geomantic disseminule form ($D_1$) conveyed by water and gravitational disseminule form favored by the development of waterside woody plants ($D_4$) seem to be better fit to this area in desseminule form. As for growth form, bunch type (t) is judged to become prevalent on the Wetlands while a good variety of phanerophytes will coexist on the earth due to artificial as well as natural disturbances.

Case of Acute Hemolytic Transfusion Reaction due to Anti-Fya Alloantibody in a Patient with Autoimmune Hemolytic Anemia (자가면역용혈환자에서 항-Fya 동종항체에 의한 급성용혈성수혈반응 1예)

  • Choi, Seung Jun;Nah, Hyunjin;Kim, Yundeok;Kim, Sinyoung;Kim, Hyun Ok
    • The Korean Journal of Blood Transfusion
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    • v.29 no.3
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    • pp.320-327
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    • 2018
  • A 72-year-old man with general weakness visited the outpatient clinic of the hematology department. The patient had been treated under the diagnosis of autoimmune hemolytic anemia for 2 years. His hemoglobin level at the time of the visit was 6.3 g/dL, and a blood transfusion was requested to treat his anemia. The patient's blood type was A, RhD positive. Antibody screening and identification test showed agglutination in all reagent cells with a positive reaction to autologous red blood cells (RBCs). He had a prior transfusion history with three least incompatible RBCs. The patient returned home after receiving one unit of leukoreduced filtered RBC, which was the least incompatible blood in the crossmatching test. After approximately five hours, however, fever, chills, dyspnea, abdominal pain, and hematuria appeared and the patient returned to the emergency room next day after the transfusion. The $anti-Fy^a$ antibody, which was masked by the autoantibody, was identified after autoadsorption using polyethylene glycol. He was diagnosed with an acute hemolytic transfusion reaction due to $anti-Fy^a$ that had not been detected before the transfusion. In this setting, it is necessary to consider the identification of coexisting alloantibodies in patients with autoantibodies and to become more familiar with the method of autoantibody adsorption.

Development of Transgenic Plant (Codonopsis lanceolata Trautv.) Harboring a Bialaphos Resistance Gene, bar (Bialaphos 저항성 유전자 bar를 이용한 형질전환 더덕개발)

  • 조광수;장정은;류종석;권무식
    • Korean Journal of Plant Tissue Culture
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    • v.26 no.4
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    • pp.281-287
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    • 1999
  • Codonopsis lanceolata ("Deoduck" in Korea) is a perennial herb, and belongs to family, Campanulaceae. Its taproot is used a good source of a wild vegetable as well as an herbaceous medicine. In this study, to develop a bialaphos-resistant transgenic Codonopsis, seed germination mechanism and somatic embryogenesis of the plant were investigated, and Agrobacterium-mediated transformation with bar gene encoding phosphinothricin acetyltransferase (PAT) was performed. Attempt were made to regenerate plant from cells via somatic embryogenesis. When the cotyledons, nodes and leaf disks were cultured on MS medium containing 2,4-D and zeatin, embryogenic calli were induced. Upon transferring the somatic embryos to N6 solid medium without plant growth regulators, they developed into plantlets under continuous illumination. All plants were dead on MS basal medium containing 10 mg/L phosphinothricin (PPT) and Basta, respectively. The explants did not produce calli in the medium containing 200 mg/L kanamycin. The explants were cocultured with Agrobacterium tumefaciens for 2 days, and transformants were selected in MS basal medium containing 1.0 mg/L 2,4-D, 100 mg/L kanamycin and 500 mg/L carbenicillin. After the selection, embryogenic calli were induced and then somatic embryos were produced by subsequent subculturing. The somatic embryos were germiated on N6 basal medium containing 200 mg/L kanamycin and 500 mg/L carbenicillin. PCR analysis showed that nptII and bar genes were introduced in the Deoduck transformants. After the confirmation of bar gene expression in RNA and protein level, the transgenic Deoduck will be used to study the genetics of filial generation with the herbicide control gene, bar.gene, bar.

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A 3-D Numerical Study on Characteristics of Flood Flow at crossroad connected asymmetrically with four roads (3차원 수치모형을 이용한 유입량 및 접근각도에 따른 도로 교차로에서의 홍수흐름특성 분석에 관한 연구)

  • Ha, Kyu-Ho;Jung, Woo-Chang;Kim, Jong-Geol
    • Proceedings of the Korea Water Resources Association Conference
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    • 2012.05a
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    • pp.557-557
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    • 2012
  • 최근 기상변화로 인한 게릴라성 집중호우 등의 증가로 재해 발생이 빈번하게 발생하고 있으며, 특히 도시지역에서의 홍수피해로 막대한 인명과 재산피해가 발생하고 있는 실정이다. 도시지역 내로 유입되는 홍수는 매우 복잡한 흐름특성을 나타내는 것으로 알려져 있다. 홍수의 대부분은 도로와 합류부를 통해 흐르므로 도시지역 내에서 홍수로 인한 피해를 저감하기 위해서는 도로 교차로에서의 홍수파의 전파특성을 분석하는 것이 중요하다. 교차로에서 흐름은 유입되는 유량이 상대적으로 적고 도로의 경사가 비교적 완만한 경우 상류 특성을 나타내나 이와 반대일 경우에는 상류 및 사류가 공존하는 복잡한 수면형을 나타낸다. (Mignot, 2005) 현재까지 국내외적으로 홍수흐름 분석에 관한 대부분의 수치적 연구에서는 주로 수직방향으로의 홍수파 변화양상에 대해서는 무시하며, 물의 압력이 정수압이라는 2차원적 가정으로 수행되어져 왔다. 그리고 대부분의 연구는 도시지역을 구성하는 건물 등이 홍수흐름에 어떤 영향을 미치는가 및 직각 교차로에 대한 분석을 수행하였으나 사각 교차로에 대한 홍수흐름에 대한 연구는 아직 미비한 실정이다. 본 연구는 3차원 수직모형을 이용하여 사각 수로 합류부에서의 상류 및 사류에 대한 흐름특성을 수리모형실험 결과와 비교분석하였다.

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Effect of O2/Ar+O2 concentration on phase stability of transparent Mn doped SnO2 monolayer film (혼합기체 O2/Ar+O2 농도 변화가 Mn 도핑된 SnO2 투명전도막의 상 안정성에 미치는 영향)

  • Kim, Taekeun;Jang, Guneik
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.31 no.4
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    • pp.154-158
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    • 2021
  • The optical transmittance of Mn-doped SnO2 monolayer film increased gradually from 80.9 to 85.4 % at 550 nm wavelengths upon increasing the O2/Ar+O2 concentration rate from 0 to 7.9 % and the band gap energy changed from 3.0 to 3.6 eV. The resistivity tended to decrease from 3.21 Ω·cm to 0.03 Ω·cm, reaching a minimum at 2.7 %, and then gradually increased from 0.03 to 52.0 Ω·cm at higher O2/Ar+O2 gas concentration ratio. Based on XPS spectra analysis, the Sn 3d5/2 peak of Mn-doped SnO2 single layer shifted slightly from 486.40 to 486.58 and O1s peak also shifted from 530.20 to 530.33 eV with increase the O2/Ar+O2 concentration ratio. Therefore, the XPS spectra results indicate that a multiphase with SnO and SnO2 coexisted in the sputtered Mn-doped SnO2 monolayer film.

Optimization of the Community Energy Supply System for D-Cube City, Multi Purpose Building (복합건물(D-Cube City) 지역에너지 공급체계 최적화)

  • Lee, Tae-Won;Kim, Yong-Ki;Lee, Kun-Woo;Lee, Ki-Bong;Cho, Dong-Ho
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.36 no.6
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    • pp.669-674
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    • 2012
  • D-Cube City is a recently completed multi purpose building consisting of four types of facilities; offices, a department store, a hotel, and congregation spaces. A community energy supply system(CES) has been installed to supply this building with electricity, steam, heat, and cold water. The BEMS, building energy management system, is currently being designed to reduce building energy consumption through the efficient operation of the various pieces of building service equipment. In this study the optimal methods for operating the CES of D-Cube City were considered. This system includes three combined heat and power systems, seven steam boilers, two hot water boilers, two absorption chillers, and four turbo chillers, and various other pieces of equipment. In result, the optimal methods of operating the CES for various energy demand levels were obtained along with the seasonal effects on the economic efficiency of the operation. The effect of the amount of energy demanded by the various facility areas on the total energy consumption was also analyzed.