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Anaerobic Ammonium Oxidation(ANAMMOX) in a Granular Sludge Reactor and its Bio-molecular Characterization  

Han, Ji-Sun (Department of Environmental Engineering, Inha University)
Park, Hyun-A (Department of Environmental Engineering, Inha University)
Sung, Eun-Hae (Department of Environmental Engineering, Inha University)
Kim, Chang-Gyun (Department of Environmental Engineering, Inha University)
Yoon, Cho-Hee (Department of Environmental Engineering, Kyungnam University)
Bae, Young-Shin (SUDOKWON Landfill Site Management Corp.)
Publication Information
Journal of Korean Society of Environmental Engineers / v.28, no.11, 2006 , pp. 1213-1221 More about this Journal
Abstract
In this study, granular sludge used in an anaerobic process treating brewery waste was inoculated in a laboratory scale of reactor to induce anaerobic ammonium oxidation(ANAMMOX). The reactor was operated with synthetic wastewater, which prepared at 1:1 ratio of $NH_4^+-N$ over $NO_2^--N$. Changes in nitrogen concentration, COD, alkalinity and gas production were analyzed. There are 3 phases of spanning in experimental period according to influent nitrogen concentration. In the Phase 1, each of the concentration of $NH_4^+-N$ and $NO_2^--N$ were increased from 1.91 $gN/m^3{\cdot}d$ to 14.29 $gN/m^3{\cdot}d$. Ammonium nitrogen loading(same as nitrite nitrogen) was 23.81 $gN/m^3{\cdot}d$ in the Phase 2 and 19.05 $gN/m^3{\cdot}d$ in the Phase 3, respectively $NO_2^--N$ has been removed up to 99% during whole period while the removal efficiency of $NH_4^+-N$ was significantly varied. In Phase 2, $NH_4^+-N$ was removed up to 75%. Microorganisms varied temporally through three phases were characterized by 16s rDNA analysis methods. ANAMMOX bacteria were dominantly found in phase 2 when the removal rate of $NO_2^--N$and $NH_4^+-N$ was the highest up to 99% and 75%, respectively. Due to erroneous exposed to air, the removal efficiency of $NH_4^+-N$ was unexpectedly lowered, but ANAMMOX bacteria still existed.
Keywords
ANAMMOX; Granular Sludge; 16s rDNA; Nitrogen Removal;
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  • Reference
1 van Dongen, L. G. J. M., Jetten, M. S. M., and van Loosdecht, M. C. M., 'The Combined Sharon/Anammox Process,' STOWA Report, IWA Publishing(2001)
2 Van de Graaf, A. A., de Bruijn. P., Robertson, L. A., Jetten, M. S. M., Robertson, L. A., and Kuenen, J. G., 'Autotrophic growth anaerobic ammonium-oxidizing micro-organism in a fluidized bed reactor,' Microbiology, 142, 2178 - 2196(1996)
3 Schmidt. I. and Bock. E., 'Anaerobic ammonia oxidation with nitrogen dioxide by Nitrosomonas eutropha,' Arch. Microbiol., 167, 106-111(1997)   DOI
4 박현아, 한지선, 김창균, '분자생물학적 방법을 이용한 매립지 미생물 군집 분석 및 안정화 지표 개발,' 대한환경공학희, 춘계학술연구발표희 논문집, 611-616(2005)
5 Mulder, A., van de Graaf, A. A., Robertson, L. A., and Kuenen, J. G., 'Anaerobic ammonium oxidation discovered in a denitrifying fluidized bed reactor,' FEMS. Microbiol. Ecol., 16, 177-184(1995)   DOI
6 황인수, 안영호, 민경석, '혐기성 슬러지상 반응조를 이용한 돈사폐수의 암모늄 제거,' 대한토목학회, 22, 615-621(2002)
7 Ficker, M., Krastel, K., Orlicky, S., and Edwards, E. 'Molecular characterization of a toluene-degrading methanogenic consortium,' Appl. Environ. Microbiol., 65(12), 5576 - 5585(1999)
8 Hovanec, T. A., Taylor, L. T., Blakis, A., Delong, E. F., 'Nitrospira-like bacteria associated with nitrite oxidation in freshwater aquaria,' Appl. Environ. Microbiol., 64, 258 - 264(1998)
9 Strous, M., Fuerst, J. A., Kramer, E. H. M., Logemann, S., Muyzer. G., van de Pas-Schoonen, K. T., Kuenen, J. G., Jetten, M. S. M., 'Missing lithotroph identified as new planctomycete,' Nature, 400, 446-449(1999)   DOI   ScienceOn
10 XU, J., TRIMBLE, J. J., and LOGAN, B. E., 'Perchlorate reduction and denitrification pathways are separate in perchlorate reducing bacteria,' In The 102nd general meeting of American Society for Microbiology, Salt Lake City, Utah.(2002)
11 Stackebrandt, E., Goebel, B. M., 'A place for DNADNA reassociation and 16S rRNA sequence analysis inthe present species definition in bacteriology,' Int. J Syst. Bacteriol., 44, 846-849(1994)   DOI   ScienceOn
12 Sabine, B., Emile, S., Georg, F., 'Genes Involved in Anaerobic Metabolism of Phenol in the Bacterium Thauera arornatica,' J. Bacterialogy, 10, 5849 - 5863(2000)
13 Robert, T. A., Helen, A. V., Irene, O-B., Charles, T. R., Philip, E. L., Richard, D., Ken, K., Sam, M., Donald, R. M., Aaron, P., David, C. W., Mary, L., Derek, R. L., 'Stimulating the In Situ Activity of Geobacter Species To Remove Uranium from the Groundwater of a Uranium-Contaminated Aquifer,' Appl. Environ. Microbiol., 10, 5884 - 5891(2003)
14 Van de Graaf, A. A., Mulder, A., de Bruijn. P., Jetten, M. S. M., Robertson, L. A., and Kuenen, J. G., 'Anaerobic oxidation of ammonium is a biologically mediated process,' Appl. Environ. Microbiol., 61, 1246-1251(1995)
15 Ahn, Y. H., Kim, H. C., Hwang, I. S., 'Nutrient removal and microbial granulation in anaerobic process treating inorganic and organic nitrogenous wastewater,' ICWNR, 04, 26-29(2004)
16 Lovley, D. R., Phillips, E. J. P., 'Bioremediation of uranium contamination with enzymatic uranium reduction,' Environ. Sci. Technol., 26, 2228 - 2234(1992)   DOI
17 Strous, M., Heijnen, J. J., Kuenen, J. G., and Jetten, M. S. M., 'The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammoniumoxidizing microorganisms,' Appl. Microbiol. Biotechnol., 50, 589 - 596(1998)   DOI
18 APHA, Standard Methods for the Examination of Water and Wastewater, 20th ed., Washington D.C., USA(1988)
19 Strous, M., Kuenen, J. G., and Jetten, M. S. M., 'Key physiology of anaerobic ammonium oxidation,' Appl. Environ. Microbiol., 65, 3248 - 3250(1999)
20 김동진, 한동우, 윤호준, 이수철, 이현용, 차기철, 유익근, 박완철, 'SBR을 이용한AMMOX 미생물 배양 및 Fluorescence in situ Hybridization에 의한 미생물 군집 분석,' 대한환경공학회지, 24, 655-663(2002)
21 de Bruijn, P., Van de Graaf, A. A., Jetten, M. S. M., Robertson, L. A., and Kuenen, J. G., 'Growth of Nitrosomonas europaea on hydroxylamine,' FEMS. Microbiol. Lett., 125, 179-184(1995)
22 Broda, E., 'Two kinds of lithotrophs missing in nature,' Z. Allg. Mikrobiol., 17, 491-493(1977)   DOI
23 Nielsen, M., Bollmann, A., Sliekers, 0., Jetten, M., Schmidt, I., Larsen, L. H., Nielsen, L. P., Revsbech, N. P., 'Kinetics, diffusional limitation and microscale distribution of chemistry and organi는 in a CANON reactor,' FEMS. Microbiol. Ecol., 51, 247 -256(2005)   DOI   ScienceOn
24 Jianlong, W., Jing, K., 'The characteristics of anaerobic ammonium oxidation(ANAMMOX) by granular sludge from an EGSB reactor,' Process Biochemistry, 40, 1973-1978( 2005)   DOI   ScienceOn
25 Helmer, C., Tromm, C., Hippen, A., Rosenwinkel, K. H., Seyfried, C. F., and Kunst, S., 'Single stage biological nitrogen removal by nitritation and anaerobic ammonium oxidation in biofilm systems,' Water Sci. Technol., 43, 311-320(2001)
26 이명숙, 박지현, '암모니아 산화세균의 분리와 그 특성,' 한수지, 31(5), 760-766(1998)