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FACTORS INFLUENCING THE FORMATION OF INSOLUBLE GLUCAN BY STREPTOCOCCUS SOBRINUS (Streptococcus sobrinus의 비수용성 글루캔 합성에 영향을 미치는 인자)

  • Chung, Jin;Kim, Shin
    • Journal of the korean academy of Pediatric Dentistry
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    • v.27 no.1
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    • pp.90-97
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    • 2000
  • There are various kinds of factors associated with the formation of dental plaque in oral cavity such as nutrient molecules and chemical agents. The factors influencing the formation of insoluble glucan by Streptococcus sobrinus and its replication were examined on orthodontic wires. The results were as follows: 1. Insoluble glucan was well produced in the media initially adjusted at pH 7.0 than pH 5.5 or pH 8.5 like bacterial replication. 2. The synthesis of insoluble glucan and bacterial replication were significantly increased in the media containing 2.5% yeast extract. The formation of insoluble glucan was inhibited by 10 folds in the media containing 20% of sucrose than 1.25%, but the replication of bacteria was increased by 20 folds. 3. Insoluble glucan was significantly formed at a concentration of 1.0mM of calcium chloride, 40mM of potassium chloride, 0.1mM of magnesium chloride, while the replication of bacteria was little influenced by them regardless their concentration. 4. The formation of insoluble glucan and bacterial replication were significant in the media containing 10mM of sodium bicarbonate, but both were completely inhibited at 100mM or above. The production of insoluble glucan and the bacterial replication were largely decreased at 10mM of Tris while insoluble glucan was formed in abundance at 100mM of Tris. 5. The synthesis of insoluble glucan and the bacterial replication were inhibited at 10mM or above of sodium phosphate and potassium phosphate.

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Effect of Fly Ash Application on the Saturated Hydraulic Conductivity of Soils with Different Soil Texture (석탄회(Fly ash) 처리가 토성(土性)이 다른 토양의 포화수리전도도(飽和水理傳導度)에 미치는 영향)

  • Kim, Jai-Joung;Hong, Soon-Dal
    • Korean Journal of Soil Science and Fertilizer
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    • v.32 no.3
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    • pp.279-284
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    • 1999
  • Fly ash is the fine ash particles that are flying out of chimney of the thermoelectric power plant where coals are used as fuel. There are two kinds of fly ashes from anthracite and bituminous coal. By scanning electron microscope(SEM) morphological feature of fly ash was confirmed to the exact spherical particles with the diameter variation from the fine to the largest about $50{\mu}m$(mainly silty particle). Surface of anthracite ash particle was very smooth but that of bituminous was somewhat coarse. To find the utilization of fly ash for improving soil permeability, soils of 4 kinds of different texture, clay, clay loam, sandy clay loam and sand mere applied with 7 levels of fly ash: 0, 10, 20, 40, 60, 80, 100%(w/w) and their saturated hydraulic conductivity(Ks) were determined at each application by constant head method. In clay soil with low water permeability, Ks value was increased about 10 times from $10^{-8}$ to $10^{-7}m\;s^{-1}$ level with application of 10% fly ash and it was slightly increased with increasing fly ash application from 40 to 80%. In clay loam Ks value was about $10^{-7}m\;s^{-1}$ level and its value was not influenced by the fly ash application. In sandy clay loam with relatively high permeability, Ks value was decreased about 10 times from $10^{-5}$ to $10^{-6}m\;s^{-1}$ level with application of 10% fly ash and also decreased about 50 times from $10^{-5}$ to $5.0{\times}10^{-7}m\;s^{-1}$ with application of more than 20% fly ash. In sand with very high permeability, Ks value was decreased about 10 times from $10^{-4}$ to $10^{-5}m\;s^{-1}$ level with application of 10% fly ash and also decreased about 100 times from $10^{-4}$ to $10^{-6}m\;s^{-1}$ level with application of 20% fly ash and continuously decreased about 500 times from $10^{-4}$ to $5.0{\times}10^{-7}m\;s^{-1}$ level with application of more than 40% fly ash. In conclusion by fly ash application saturated hydraulic conductivity was increased in clay soil, on the contrary it was decreased in sandy soils. Fly ash may be used as a material for amelioration of soil permeability.

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Effect of Nicotinic Acid on Sperm Characteristic and Oocyte Development after In Vitro Fertilization using Cryopreserved Boar Semen

  • Kim, Yu-Jin;Lee, Sang-Hee;Lee, Yeon-Ju;Oh, Hae-In;Cheong, Hee-Tae;Yang, Boo-Keun;Lee, Seunghyung;Park, Choon-Keun
    • Journal of Embryo Transfer
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    • v.30 no.1
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    • pp.7-15
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    • 2015
  • The objective of this study was to investigate the efficiency of nicotinic acid on sperm cryosurvival and fertilization ability in frozen-thawed boar semen. Boar semen was collected by glove-hand method and was frozen using freezing solution treated to 0, 5, 10 and 20 mM of nicotinic acid. The frozen sperm for sperm characteristic analysis was thawed such as viability, acrosome reaction, and mitochondrial integrity. The frozen-thawed sperm was estimated by SYBR14/PI double staining for viability, FITC-PNA/PI double staining for acrosome reaction and Rhodamine123/PI double staining for mitochondrial integrity using a flow cytometry. The embryo was estimated in vitro development and DCFDA staining for reactive oxygen species assessment. As results, frozen-thawed sperm viability was significantly higher in 5 and 10 mM ($61.1{\pm}1.5%$,$64.7{\pm}2.0%$) of nicotinic acid than other groups (0 mM, $52.1{\pm}2.3%$; 20 mM, $47.8{\pm}5.1%$, P<0.05). The live sperm with acrosome reaction was significantly higher in 5 and 10 mM of nicotinic acid ($26.1{\pm}1.8%$, $24.9{\pm}1.5%$) than other groups (0 mM, $35.3{\pm}0.8%$; 20 mM, $36.5{\pm}1.9%$, P<0.05). The live sperm with mitochondrial integrity was significantly higher in 5 and 10 mM ($84.2{\pm}3.6%$, $88.4{\pm}2.3%$) of nicotinic acid than other groups (0 mM, $77.3{\pm}4.4%$; 20 mM, $73.3{\pm}3.6%$, P<0.05). Blastocyst rate of in vitro development was significantly higher in 10 mM ($17.0{\pm}1.3%$) of nicotinic acid than other groups (0 mM, $9.4{\pm}0.5%$; 5mM, $12.6{\pm}0.8%$; 20 mM, $5.0{\pm}1.0%$, P<0.05). Moreover, total cell number was higher in 5 and 10 mM ($53.6{\pm}2.9%$, $57.9{\pm}2.8%$) of nicotinic acid than other groups (0 mM, $41.0{\pm}1.4%$; 20 mM, $23.2{\pm}2.8%$, P<0.05). Hydrogen peroxide in embryos was lower in 5 mM nicotinic acid ($0.7{\pm}0.1%$) than other groups (0 mM, $1.0{\pm}0.1%$; 10mM, $0.9{\pm}0.0%$; 20 mM, $1.4{\pm}1.0%$, P<0.05). In conclusion, nicotinic acid-treated semen improves cryosurvival and quality of spermatozoa. Also, the fertilized oocytes with nicotinic acid improve quality of embryo and blastocyst formation.

Changes in Salinity, Hydraulic Conductivity and Penetration Resistance of a Silt Loam Soil in a Reclaimed Tidal Land (미사질 양토인 간척지 토양에서의 염류도와 수리전도도 및 관입 저항의 변화)

  • Jung, Yeong-Sang;Yoo, Sun-Ho;An, Yeol;Joo, Jin-Ho;Yu, Il-Ho
    • Korean Journal of Soil Science and Fertilizer
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    • v.35 no.4
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    • pp.207-215
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    • 2002
  • Changes in salinity, hydraulic conductivity and penetration resistance in a reclaimed tidal land reclaimed in 1986 were studied. The salinity monitoring based on electrical conductivity of saturated extract, ECe, was conducted from 1994, when the land use for experimental crop production started after tile drainage. The site was abandoned since 1999. The hydraulic conductivity was measured by a sand fill auger hole method, and the resistance was measured with a dynamic penetrometer in situ. The averaged ECe in 1994 was $33.7dS\;m^{-1}$ ranging from 25.5 to $44.8dS\;m^{-1}$, and was decreased to $25.7dS\;m^{-1}$ with large range from 0.8 to $70.3dS\;m^{-1}$ before experiment was $1.89{\times}10^{-7}m\;s^{-1}$. It increased to $1.32{\times}10^6m\;s^{-1}$ in the top 20-cm soil with large variability, while it showed $3.44{\times}10^7m\;s^{-1}$ beneath the 20-cm soil depth with less variability. The penetration resistance of the soil ranged from 0.05 to 9.99MPa. The vertical distribution of penetration resistance indicated the hardened layer was developed at the depth of 20~40 cm where the hydraulic conductivity was sharply decreased.

The study of high chloride in the coastal area of Cheju island (제주도 해안지역 고염분 현상에 대한 고찰)

  • 한규언;신희섭
    • The Journal of Engineering Geology
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    • v.10 no.2
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    • pp.150-171
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    • 2000
  • In the Cheju Island, there are high salinity water yield in the coastal area. There are various reason; the main reason is low-groundwater level by the structure of geology, and over-yielding etc. This study analysis water quality, the distribution of high salinity wells. 16 sample well logged the electronic geology survey. As result of distribution of Cl$^-$ is the East Area is high than other areas. Water quality test data divides 6 group by sea level of well bottom: over Om, Om~-1Om, -10~-20m, -20~-30m, -30~-40m, under -40m. According collect data of Kriging, and logged the electronic geology survey, and other survey are related chlorine(Cl$^-$). The map of Cl$^-$ distribution was made. Bukcheju Gun, Hallim Up, there are 2 wells prevent high salinity water. Both of well are effective grout sill salinity water intrusion aquifer.

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A Study on the Influence Area of Excavation around Railroads (철로 주변의 지하굴착 영향권에 관한 연구)

  • Park, Jong-Su;Jang, Jeong-Wook;Park, Choon-Sik
    • Proceedings of the Korean Geotechical Society Conference
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    • 2006.03a
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    • pp.1032-1037
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    • 2006
  • This thesis studied effects of the excavation around railroads on the deformation of the lateral ground and neighboring railroads. The conclusions of the study are as follows. 1. When the depth of excavationis 10m, the influential area should be 35m for soft clay, 20m for normal clay, 15m for hard clay, 15m for loose sand, 12m for slightly dense sand, and 8m for dense sand. 2. When the influential area is 10m, the allowable excavation depth should be 2.5m for soft clay, 4.8m for normal clay, 7.5m for hard clay, 7.2m for loose sand, 8.8m for slightly dense sand, and 10m for dense sand. 3. When the influential area is 20m, the allowable excavation depth should be 4.5m for soft clay, and up to 10m for the other five kinds of soil. 4. When the influential area is 30m, the allowable excavation depth should be 7.5m for soft clay, and up to 10m for the other five kinds of soil. 5. When the influential area is 35m, the allowable excavation depth should be up to 10m for all kinds of soil.

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OPTICAL PROPERTIES Of SEA WATER IN THE NORTHWEST PACIFIC (북서태평양에서의 해수의 광학적 성질)

  • YANG Yong Rhim
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.10 no.4
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    • pp.237-241
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    • 1977
  • Optical properties were studied in the Northwest Pacific near Kamchatka Peninsula based on ten oceanographic stations from September 20 to 24, 1976. Submarine light intensity was measured by usins a submarine illuminometer (RIGO, Type: 2501-A) ; equipped with a red filter (RIGO, Type: V-R-60, wave length: 600-620 nm). Light intensity in the upper 40 m depth layer was measured at 1 m depth intervals. The absorption coefficient for red color in the area ranged from 0. 178 to 0.376 (mean 0.278) : the Secchidisc depth in the area ranged from 9 to 12 meters (mean 10.6 meters). The relationship between absorption coefficient (m) and transparency depth (D) was m=5.347/D. The rates of light penetration for red color at three different depths are computed with reference to the surface light intensity. The mean rates of light penetration were $16.36\%\;(6.45\~23.5\%),\;3.65\%\;(1.38\~7.31\%)\;and\;0.276\%(0.048\~0.647\%) $ at the depths of s m, 10 m, and 20 m, respectively.

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The Study on The Skil Development of Optical CT Using Faraday Effects (Faraday효과를 이용한 광CT 기술 개발 연구)

  • Jeon, Jea-Il;Ahn, Mi-Kyoung;Park, Won-Zoo;Lee, Kwang-Sik;Kim, Jung-Bae;Kim, Min-Soo
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.18 no.3
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    • pp.109-113
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    • 2004
  • In this paper, we took the basic experiment in order to explore the optical CT(optical current transformer) for measuring high current in a superhigh voltage condition. It is based on the faraday effect that changes the polarization characteristic of optical signals by the magnetic field. We used 1310[nm] laser diode for a light source and comprised a sensing part with the optional fiber of 5[m], 10[m], 20[m] length each. The range of current was from 400[A] to 1300[A]. We observed the characteristic of output that changes according to the length of the optical fiber composing the sensing part. The optical fiber of 5[m] length used for the sensing part had -11.1% of the maximum ratio of error and 10[m] and 20[m] length had -3.5% and 1.7% each. This means that the power and linearity of output increase in proportion to the length of optical fiber and applied current in the same experimental condition.

Extraction of Individual Trees and Tree Heights for Pinus rigida Forests Using UAV Images (드론 영상을 이용한 리기다소나무림의 개체목 및 수고 추출)

  • Song, Chan;Kim, Sung Yong;Lee, Sun Joo;Jang, Yong Hwan;Lee, Young Jin
    • Korean Journal of Remote Sensing
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    • v.37 no.6_1
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    • pp.1731-1738
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    • 2021
  • The objective of this study was to extract individual trees and tree heights using UAV drone images. The study site was Gongju national university experiment forest, located in Yesan-gun, Chungcheongnam-do. The thinning intensity study sites consisted of 40% thinning, 20% thinning, 10% thinning and control. The image was filmed by using the "Mavic Pro 2" model of DJI company, and the altitude of the photo shoot was set at 80% of the overlay between 180m pictures. In order to prevent image distortion, a ground reference point was installed and the end lap and side lap were set to 80%. Tree heights were extracted using Digital Surface Model (DSM) and Digital Terrain Model (DTM), and individual trees were split and extracted using object-based analysis. As a result of individual tree extraction, thinning 40% stands showed the highest extraction rate of 109.1%, while thinning 20% showed 87.1%, thinning 10% showed 63.5%, and control sites showed 56.0% of accuracy. As a result of tree height extraction, thinning 40% showed 1.43m error compared with field survey data, while thinning 20% showed 1.73 m, thinning 10% showed 1.88 m, and control sites showed the largest error of 2.22 m.

Foliar Colonization and Growth Promotion of Red Pepper (Capsicum annuum L.) by Methylobacterium oryzae CBMB20

  • Lee, Min-Kyoung;Chauhan, Puneet Singh;Yim, Woo-Jong;Lee, Gyeong-Ja;Kim, Young-Sang;Park, Kee-Woong;Sa, Tong-Min
    • Journal of Applied Biological Chemistry
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    • v.54 no.2
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    • pp.120-125
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    • 2011
  • In order to exploit Methylobacterium oryzae CBMB20 as of plant growth promoting agent, different inoculation methods have been evaluated. The present study aimed to evaluate soil, foliar, and soil+foliar inoculations of M. oryzae CBMB20 to improve the growth, fruit yield, and nutrient uptake of red pepper (Capsicum annuum L.) under greenhouse conditions. The population range of green fluorescent protein (gfp)-tagged M. oryzae CBMB20 using the three inoculation methods was 2.5-2.9 ${\log}_{10}$ cfu/g in the rhizosphere and 4.5-6.0 ${\log}_{10}$ cfu/g in the phyllosphere of red pepper plants. Confocal laser scanning microscopy results confirmed the colonization of M. oryzae CBMB20 endophytically on leaf surface. Plant height, fruit dry weight, and total biomass were significantly higher ($p{\leq}0.05$) in all M. oryzae CBMB20 inoculation methods as compared to non-inoculated control. Furthermore, uptake of mineral nutrients such as N, P, K, Ca, and Mg in red pepper plants in all M. oryzae CBMB20 inoculation methods was higher than in non-inoculated control. Comparative results of inoculation methods clearly demonstrated that soil+foliar inoculation of M. oryzae CBMB20 lead to the highest biomass accumulation and nutrient uptake which may be due to its efficient colonization in the red pepper rhizosphere and phyllosphere.