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점망둑 (Chasmichthys dolichognathus)의 생식 스테로이드 호르몬 분석

  • 박명희;백혜자;유명숙
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2002.10a
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    • pp.253-254
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    • 2002
  • 경골어류의 난소 성숙 과정은 여포층에서 생성ㆍ분비되는 성 스테로이드 호르몬에 의해 조절된다. 성 스테로이드 호르몬 중에서도 C2l-스테로이드가 난소 성숙 과정에 중요한 역할을 하는 것으로 알려져 있으며, 특히 20$\alpha$와 20$\beta$-hydroxy group을 가지고 있는 스테로이드, 17 $\alpha$-hyoxy, 20 $\alpha$-dihydroprogesterone (17 $\alpha$ 20 $\alpha$ OHP)과 17$\alpha$-hyoxy, 20$\beta$-dihydroprogesterone (17$\alpha$20$\beta$OHP)가 성숙유도에 가장 효과적인 것으로 보고하고 있다 (Canario and Scott, 1989; 1990). (중략)

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The Theory of Tokyo in the 20th Century as a Garden City

  • Naito, Keita
    • Journal of East-Asian Urban History
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    • v.1
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    • pp.99-134
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    • 2019
  • In the Edo period, there were many samurai residences with gardens in Edo. In the 20th century, some of these gardens were inherited and new gardens were also cultivated in Tokyo. Because of this, Tokyo in the 20th century has been a garden city since the Edo period. This study shows the characteristics of gardens inherited today from the 20th century and the succession process of these gardens in the 20th century.

Biosynthesis of $17\alpha-hydroxy$, $20\alpha-dihydroprogesterone$ by Ovaries of the Spotted Flounder (Verasper variegatus)

  • Baek Hea-Ja
    • Fisheries and Aquatic Sciences
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    • v.4 no.2
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    • pp.70-74
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    • 2001
  • To examine the production of steroids with potential oocyte maturation-inducing activity in the spotted flounder, Verasper variegatus, we have incubated post-vitellogenic oocytes (0.82­0.95mm in diameters) with radiolabeled pregnenolone and $17\alpha-hydroxyprogesterone$. The resulting metabolites were analyzed by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). The two main metabolites (progestogens) found in both incubations co-migrated with $17\alpha-hydroxy$, $20\alpha-dihydroprogesterone$ $(17\alpha, 20\alpha OHP)$ and $17\alpha-hydroxy,\;$20\beta-dihydroprogesterone$ (17 a20{30HP). Additional chromatography by HPLC and TLC confirmed the presence of radioactive $17\alpha, 20\alpha OHP$ and a large amount of unknown metabolite. The present study did not reveal in vitro formation of $l7\alpha 20\beta OHP$. Although 1$l7\alpha 20\beta OHP$ was found in a small amount, the synthesis of this steroid suggests that it may play a role in regulating the oocyte maturation process in the spotted flounder.

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Complete Assignment of $^1H$- and $^{13}C-NMR$ in (20R)-panaxadiol and (20R)-panaxatriol ((20R)-파낙사디올과 (20R)-파낙사트리올에 대한 $^1H$- 및 $^{13}C-NMR$의 완전동정)

  • Kim, Dong-Seon;Baek, Nam-In;Park, Jong-Dae;Lee, You-Hui;Kim, Shin-Il
    • YAKHAK HOEJI
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    • v.40 no.3
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    • pp.293-299
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    • 1996
  • The $^1H$- and $^{13}C$-NMR signals of (20R)-panaxadiol and (20R)-panaxatriol were completely assigned by the extensive application of modern 2D-NMR techniques, $^1H-^1H$ COSY, HMQC and HMBC.

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Bioinformatics Analysis of Hsp20 Sequences in Proteobacteria

  • Heine, Michelle;Chandra, Sathees B.C.
    • Genomics & Informatics
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    • v.7 no.1
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    • pp.26-31
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    • 2009
  • Heat shock proteins are a class of molecular chaperones that can be found in nearly all organisms from Bacteria, Archaea and Eukarya domains. Heat shock proteins experience increased transcription during periods of heat induced osmotic stress and are involved in protein disaggregation and refolding as part of a cell's danger signaling cascade. Heat shock protein, Hsp20 is a small molecular chaperone that is approximately 20kDa in weight and is hypothesized to prevent aggregation and denaturation. Hsp20 can be found in several strains of Proteobacteria, which comprises the largest phyla of the Bacteria domain and also contains several medically significant bacterial strains. Genomic analyses were performed to determine a common evolutionary pattern among Hsp20 sequences in Proteobacteria. It was found that Hsp20 shared a common ancestor within and among the five subclasses of Proteobacteria. This is readily apparent from the amount of sequence similarities within and between Hsp20 protein sequences as well as phylogenetic analysis of sequences from proteobacterial and non-proteobacterial species.

Dielectric Properties of PZT(20/80)/PZT(80/20) Heterolayered Thick Films (PZT(20/80)/PZT(80/20) 이종층 후막의 유전특성)

  • Lee, Sung-Gap;Lee, Young-Hie;Bae, Seon-Gi
    • Proceedings of the KIEE Conference
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    • 2005.07c
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    • pp.1918-1920
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    • 2005
  • PZT(20/80) and PZT(80/20) powders, prepared by the sol-gel method, were mixed with an organic vehicle and the PZT(20/80)/PZT(80/20) heterolayered thick films were fabricated by the screen-printing method on $Pt/Al_2O_3$ substrates. The structural properties such as DTA, X-ray diffraction and microstructure, were examined as a function of the sintering temperature. In the DTA analysis, the formation of the polycrystalline perovskite phase was observed at around $880^{\circ}C$. The average thickness of the PZT heterolayered thick films was approximately $80-90{\mu}m.$

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The Reduction Effect of Yeast Hydrolysate SCP-20 on Premenstrual Syndrome (효모 가수분해물 SCP-20의 월경전 증후군 감소효과)

  • 유광원;오성훈;최윤석;황원준;서형주
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.5
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    • pp.1000-1003
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    • 2001
  • A yeast hydrolysate SCP-20 with anti-stress and mitigative effects for premenstrual syndromes (PMS) was composed with crude protein (57.5%) and carbohydrates (28.1%). For the investigation of anti-stress effect in the immobilization stressed rats, the weights of spleen, thyroid and kidney in the group of SCP-20 sdministration were significantly different from those in the group not given SCP-20. Total power (TP), suppression indicator of stress, was obtained from frequency of heart rate variability. TP was changed by the administration of SCP-20 suggesting that SCP-20 has the autonomous nervous control effect. In premenstrual assessment form(PAF), the administration of SCP-20 reduced the intensity of somatic symptoms, emotional symptoms, and behavioral symptoms.

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Effects of Protected Conjugated Linoleic Acid Supplementation on Milk Fatty Acid in Dairy Cows

  • Piamphon, N.;Wachirapakorn, Chalong;Wanapat, M.;Navanukraw, C.
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.1
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    • pp.49-56
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    • 2009
  • The objective was to determine the effects of supplementation of protected conjugated linoleic acid (CLA), CLA-20 comprising 10% each of cis-9, trans-11 and trans-10, cis-12, on milk production and fatty acid profiles in plasma and milk in lactating dairy cows. Five mid-lactation, multiparous crossbred Holstein Friesian cows with average 402${\pm}$20 kg BW were used in a 5${\times}$5 Latin square design for 21-d periods. Cows were given a total mixed ration (TMR) and supplemented with CLA-20 at 0, 20, 40, 80 and 160 g/d. The results showed that dry matter intake depression occurred in cows supplemented with CLA-20 at 160 g/d. Milk production slightly increased when CLA-20 supplementation was at 20, 40 and 80 g/d. However, 3.5% fat-corrected milk (FCM) was not affected by CLA-20 supplementation. Increased levels of CLA-20 supplementation resulted in a significantly decreased percentage of milk fat. Plasma concentrations of fatty acid were not altered by the amounts of CLA-20 supplementation except for the concentration of trans-10, cis-12 CLA. For all dietary treatments, percentages of fatty acids (C4:0, C6:0, C8:0, C13:0, C14:0 C14:1 C15:0 C15:1 C16:0, C16:1, C18:1n9t, C18:2n6t, C18:2n6c, C20:0, C18:3n6, C18:3n3, C20:1 and C20:3n6) in milk fat were similar. Concentrations of C10:0, C11:0, C12:0 and C18:1n9c were decreased cubically and C18:0 was elevated linearly (p<0.01) according to the increased amounts of CLA-20 supplemented. The linear increase was observed for cis-9, trans-11 CLA (0.62, 1.17, 1.94, 1.87 and 1.82% of total fatty acid), trans-10, cis-12 CLA (0.01, 0.63, 0.67, 0.93 and 0.95% of total fatty acid) and total CLA (0.80, 2.25, 3.16, 3.97 and 3.94% of total fatty acid) in milk fat from 0 to 160 g/d of CLA-20 supplement. In conclusion, concentration of cis-9, trans-11 CLA in milk fat was concomitantly elevated at an increasing rate with the increased amounts of CLA-20. Based on the results in this study, supplementation of CLA-20 at 80 g/d optimally enhanced total CLA in milk fat.

Nitrogen Split Application of Direct-seeded Rice in Tillage and No-tillage Systems (경운과 무경운 조건에서 벼 건답휴립직파재배의 질소분시비율)

  • 이석순;홍승범;백준호
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.5
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    • pp.405-412
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    • 1992
  • Growth characters of dry seeded paddy rice was studied at different levels of nitrogen(N) split application (basal : immediately after irrigation : 2 weeks after irrigation: 25 days before heading: heading stage with 10 : 20 : 30 : 20 : 20%, 20 : 30 : 20 : 20 : 10%, 30 : 40 : 0 : 20 : 10%, and 100% basal of slow release urea) in tillage and no-tillage systems. On May 17 in 1990, 6kg /10a of dry seeds of a breeding line of Milyang 95 was broadcasted and covered with a power tiller. Between tillage and no-tillage systems yield and its components, lodging related characteristics, and contents of cellulose, hemicellulose and lignin of culm base were similar. Leaf area index at heading stage was higher in tillage, but lodging index was lower compared with those in no-tillage plot. Culm length, effective tiller ratio, culm base weight, 1000-grain weight, harvest index in slow release N applied plot were higher compared with those in other N treatments. Grain yield of rice among levels of N split applications was similar in tillage, but it was higher in slow release N applied plot in no-tillage if 1kg /10a of additional N was applied as urea at panicle formation stage. At early growth stage N concentration and N uptake were lower in the slow release N applied plot, but higher after heading stage compared with other N treatments. The number of tillers was lowest in slow release N applied plot during the tillering stage, but the number of panicles per hill was similar to other N treatments.

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Production of $C_{21}$-Steroids in Longchin Goby, Chasmichthys dolichognathus during Oocyte Maturation (점망둑(Chasmichthys dolichognathus)의 성숙기 난모세포에서의 $C_{21}$-스테로이드 생성)

  • Baek, Hea-Ja
    • Development and Reproduction
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    • v.12 no.1
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    • pp.107-112
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    • 2008
  • To investigate the $C_{21}$-steroids produced from maturating oocytes in the longchin goby, Chasmichthys dolichognathus, the oocytes ($0.74{\sim}0.97\;mm$) were incubated with radiolabeled $17{\alpha}$-hydroxyprogesterone ($^3H-17{\alpha}OHP$) for 24 hours. The resulting metabolites were analyzed by thin layer chromatography and identified by gas chromatography-mass spectrometry. Two $C_{21}$-steroids, $17{\alpha}$-hydroxy, $20{\alpha}$-dihydroprogesterone ($17{\alpha}20{\alpha}P$) and $17{\alpha}$-hydroxy, $20{\beta}$-dihydroprogesterone ($17{\alpha}20{\beta}P$), were converted from $^3H-17{\alpha}OHP$ in the maturing oocytes. These two main metabolites were detected at 0.80 mm diameter oocytes or greater. In addition, the effects of these metabolites on in vitro germinal vesicle breakdown (GVBD) were tested. The sensitivity of oocytes to the induction of GVBD was greater at $17{\alpha}20{\beta}P$ than $17{\alpha}20{\alpha}P$. This result showed that $17{\alpha}20{\beta}P$ is a major maturation inducing steroid (MIS) in longchin goby, suggesting $17{\alpha}20{\alpha}P$ may play a role in regulating the oocyte maturation process.

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