• 제목/요약/키워드: 20(S)-$Rg_3$

검색결과 119건 처리시간 0.023초

입자크기에 따른 SnO2:Ni 가스센서의 감응 특성 (Effect of the Particle Size of SnO2:Ni on Gas Sensing Properties)

  • 이지영;유일
    • 한국재료학회지
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    • 제21권4호
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    • pp.207-211
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    • 2011
  • Ni 8 wt.%-doped tin oxide ($SnO_2$) thick films were fabricated into gas sensors by the method of screen printing onto alumina substrates. The particle size of $SnO_2$ was controlled by changing the ball-mill time between 0~120 h. The structural and morphological properties of these thick films were investigated using X-ray diffraction and scanning electron microscopy. The structural properties of $SnO_2$ powders showed a tetragonal phase with (110) dominant orientation. The particle size of the $SnO_2$:Ni powders after ball-mill of 120 h was about 0.05 ${\mu}m$. The gas sensitivity (S = Rg/Ra) to 5 ppm $CH_4$ gas and $CH_3CH_2CH_3$ gas was measured at room temperature by comparing the resistance in air (Ra) with that of the target gases (Rg). The sensitivity of the $SnO_2$ gas sensors was enhanced by increasing the ball-mill time. There was an association between the sensitivity of both the $CH_4$ gas and the $CH_3CH_2CH_3$ gas and the particle size of the $SnO_2$. $SnO_2$ gas sensors prepared by 72 h ball-mill showed a sensitivity of about 13 to 5 ppm $CH_4$ gas and $CH_3CH_2CH_3$ gas. The response time of the $SnO_2$:Ni gas sensors to the $CH_4$ gas was about 20 seconds.

Ginsenoside Rk1 suppresses platelet mediated thrombus formation by downregulation of granule release and αIIbβ3 activation

  • Shin, Jung-Hae;Kwon, Hyuk-Woo;Irfan, Muhammad;Rhee, Man Hee;Lee, Dong-Ha
    • Journal of Ginseng Research
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    • 제45권4호
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    • pp.490-497
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    • 2021
  • Background and objective: Synthetic ginsenoside compounds G-Rp (1,3, and 4) and natural ginsenosides in Panax ginseng 20(S)-Rg3, Rg6, F4 and Ro have inhibitory actions on human platelets. However, the inhibitory mechanism of ginsenoside Rk1 (G-Rk1) is still unclear thus, we initiated investigation of the anti-platelet mechanism by G-Rk1 from Panax ginseng. Methodology: Our study focused to investigate the action of G-Rk1 on agonist-stimulated human platelet aggregation, inhibition of platelet signaling molecules such as fibrinogen binding with integrin αIIbβ3 using flow cytometry, intracellular calcium mobilization, fibronectin adhesion, dense granule secretion, and thromboxane B2 secretion. Thrombin-induced clot retraction was also observed in human platelets. Key Results: Collagen, thrombin, and U46619-stimulated human platelet aggregation were dose-dependently inhibited by G-Rk1, while it demonstrated a more effective suppression on collagen-stimulated platelet aggregation using human platelets. Moreover, G-Rk1 suppressed collagen-induced elevation of Ca2+ release from endoplasmic reticulum, granule release, and αIIbβ3 activity without any cytotoxicity. Conclusions and implications: These results indicate that G-Rk1 possess strong anti-platelet effect, proposing a new drug candidate for treatment and prevention of platelet-mediated thrombosis in cardiovascular disease.

Gypenoside V로부터 minor ginsenosides의 생산 (Production of Minor Gisenosides from Gypenoside V)

  • 손나리;민진우;장미;김효연;전지나;양덕춘
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2010년도 정기총회 및 추계학술발표회
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    • pp.20-20
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    • 2010
  • Panax ginseng C.A Meyer is frequently taken orally as a traditional herbal medicine in Asian countries. The major components of ginseng are ginsenoside, which are pharmaceutical activity. The six major ginsenosides, including Rb1, Rb2, Rc, Rd, Re and Rg1 account for 90% of total ginsenosides. Even though the minor ginsenosides, including Rg3, Rh2 and compound K has high pharmacetical activities, the price of minor ginsenosides is too high. Therefore we isolated the gypenoside V and made it converted to minor ginsenosides. In the plant Gynostemma pentaphyllum Makino, gypenosdie V was presented as dominant saponin (content about 2.4%), and was similar to protopanaxadol type ginsenosides such as ginsenoside Rb1. In this study, we confirmed that the coversion of gypenoside V to minor ginsenosides after using the various treatment such as heating, acid treatment, commercial edible enzyme, and lactobacillus. Consequently, we optimizied the transformation of gypenoside V to minor ginsenoside using Thin Layer Chromatography (TLC), High Performance Liquid Chromatography (HPLC), Time-of-flight Mass Spectrometry (LC/TOF/MS).

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흑삼의 제조 및 항암효과 (Preparation of Black Ginseng and its Antitumor Activity)

  • 이지현;신귀남;김의검;신현중;명창선;오한진;김동희;노성수;조원;서영배;박용진;강철우;송규용
    • 동의생리병리학회지
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    • 제20권4호
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    • pp.951-956
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    • 2006
  • The purpose of this study is to prepare black ginseng and evaluate its antitumor activity. In order to achieve such aim, 5 year fresh ginsenges were steamed at 95'E for 3 hr in pottery apparatus and dried at $60^{\circ}C$ for 12-36 hr. This process was repeated again nine times in same condition. Among the ginseng saponins in black ginseng, the amount of Ginsenoside $Rg_3$ was examined by HPLC. 10.05 mE of Ginsenoside $Rg_3$ was obtained from 1 g of dried black ginseng prepared. The extract of black ginseng exhibited stronger cytotoxic activity against MCF-1, HT-1080 and Hepa 1C1C7 tumor cell lines in vitro than the extract of red ginseng. Also, the extract of black ginseng exhibited stronger antitumor activity(33%) in BDFl mice bearing Lewis lung carcinoma cells(LLC) than the extract of red ginseng(23%). From these results, it was concluded that Black ginseng had antitumor activity suggesting its application for the prevention and treatment of cancer.

A Structure-Function Relationship Exists for Ginsenosides in Reducing Cell Proliferation and Inducing Apoptosis in THP-1 Cells

  • Popovich David G.;Kitts David D.
    • 고려인삼학회:학술대회논문집
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    • 고려인삼학회 2002년도 학술대회지
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    • pp.545-555
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    • 2002
  • Ginsenosides of 20(S)-protopanaxadiol and 20(S)-protopanaxatriol classification including the aglycones, PD, PI and ginsenosides Rh2, Rhl were shown to posses characteristic effects on proliferation of THP-l human leukaemia cells. A similar result was not apparent for ginsenoside Rg3 or dexamathasone. The concentration to inhibit $50\%$ of cells $(LC_{50})$ for PD, Rh2, PI and Rhl were 13 ${\mu}g/mL,\;15{\mu}g/mL,\;19{\mu}g/mL\;and\;210\;{\mu}g/mL$ respectively. Cell cycle analysis showed apoptosis with PD and PI treatment of THP-1 cells resulting in a build up of sub-G1 cells after 24, 48 and 72 hours of treatment. Rh2, and dexamathasone treatments also increased apoptotic cells after 24 hours, where as Rhl did not. After 48 and 72 hours Rh2, Rhl and dexamathasone similarly increased apoptosis, but these effects were significantly (P<0.05) lower than observed for both PD and PI treatments. Furthermore, treatments that produced the largest build up of apoptotic cells were also found to have the largest release of lactate dehydrogenase (LDH). It can be concluded from these studies that the presence of sugars to PD and PI aglycone structure reduces the potency to induce apoptosis, and alternately alter membrane integrity. These cytotoxic effects to THP-l cells were different from dexamethasone.

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Comparative Study of White and Steamed Black Panax ginseng, P. quinquefolium, and P. notoginseng on Cholinesterase Inhibitory and Antioxidative Activity

  • Lee, Mi-Ra;Yun, Beom-Sik;Sung, Chang-Keun
    • Journal of Ginseng Research
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    • 제36권1호
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    • pp.93-101
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    • 2012
  • This study evaluated the anti-cholinesterases (ChEs) and antioxidant activities of white ginseng (WG) and black ginseng (BG) roots of Panax ginseng (PG), P. quinquefolium (PQ), and P. notoginseng (PN). Ginsenosides $Rg_1$, Re, Rf, $Rb_1$, Rc, $Rb_2$, and Rd were found in white PG, whereas Rf was not found in white PQ and Rf, Rc, and $Rb_2$ were not detected in white PN. The major ginsenoside content in steamed BG including $RK_3$, $Rh_4$, and 20(S)/(R)-$Rg_3$ was equivalent to approximately 70% of the total ginsenoside content. The WG and BG inhibited acetylcholinesteras (AChE) and butyrylcholinesterase (BChE) in a dose dependent manner. The efficacy of BG roots of PG, PQ, and PN on AChE and BChE inhibition was greater than that of the respective WG roots. The total phenolic contents and 2, 2-diphenyl-1-picryl-hydrazyl (DPPH) scavenging activity were increased by heat treatment. Among the three WG and BG, white PG and steamed black PQ have significantly higher contents of phenolic compounds. The best results for the DPPH scavenging activity were obtained with the WG and BG from PG. These results demonstrate that the steamed BG roots of the three studied ginseng species have both high ChEs inhibition capacity and antioxidant activity.

THEORETICAL STUDY ON OBSERVED COLOR-MAGNITUDE DIAGRAMS

  • Lee, See-Woo
    • 천문학회지
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    • 제12권1호
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    • pp.41-70
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    • 1979
  • From $B\ddot{o}hm$-Vitense's atmospheric model calculations, the relations, [$T_e$, (B-V)] and [B.C, (B-V)] with respect to heavy element abundance were obtained. Using these relations and evolutionary model calculations of Rood, and Sweigart and Gross, analytic expressions for some physical parameters relating to the C-M diagrams of globular clusters were derived, and they were applied to 21 globular clusters with observed transition periods of RR Lyrae variables. More than 20 different parameters were examined for each globular cluster. The derived ranges of some basic parameters are as follows; $Y=0.21{\sim}0.33,\;Z=1.5{\times}10^{-4}{\sim}4.5{\times}10^{-3},\;age,\;t=9.5{\sim}19{\times}10^9$ years, mass for red giants, $m_{RG}=0.74m_{\odot}{\sim}0.91m_{\odot}$, mass for RR Lyrae stars, $m_{RR}=0.59m_{\odot}{\sim}0.75m_{\odot}$, the visual magnitude difference between the turnoff point and the horizontal branch (HB), ${\Delta}V_{to}=3.1{\sim}3.4(<{\Delta}V_{to}>=3.32)$, the color of the blue edge of RR Lyrae gap, $(B-V)_{BE}=0.17{\sim}0.21=(<(B-V)_{BE}>=0.18),\;[\frac{m}{L}]_{RR}=-1.7{\sim}-1.9$, mass difference of $m_{RR}$ relative to $m_{RG},(m_{RG}-m_{RR})/m_{RG}=0.0{\sim}0.39$. It was found that the ranges of derived parameters agree reasonably well with the observed ones and those estimated by others. Some important results obtained herein can be summarized as follows; (i) There are considerable variations in the initial helium abundance and in age of globular clusters. (ii) The radial gradient of heavy element abundance does exist for globular clusters as shown by Janes for field stars and open clusters. (iii) The helium abundance seems to have been increased with age by massive star evolution after a considerable amount (Y>0.2) of helium had been attained by the Big-Bang nucleosynthesis, but there is not seen a radial gradient of helium abundance. (iv) A considerable amount of heavy elements ($Z{\sim}10{-3}$) might have been formed in the inner halo ($r_{GC}$<10 kpc) from the earliest galactic co1lapse, and then the heavy element abundance has been slowly enriched towards the galactic center and disk, establishing the radial gradient of heavy element abundance. (v) The final galactic disk formation might have taken much longer by about a half of the galactic age than the halo formation, supporting a slow, inhomogeneous co1lapse model of Larson. (vi) Of the three principal parameters controlling the morphology of C-M diagrams, it was found that the first parameter is heavy clement abundance, the second age and the third helium abundance. (vii) The globular clusters can be divided into three different groups, AI, BI and CII according to Z, Y an d age as well as Dickens' HB types. BI group clusters of HB types 4 and 5 like M 3 and NGC 7006 are the oldest and have the lowest helium abundance of the three groups. And also they appear in the inner halo. On the other hand, the youngest AI clusters have the highest Z and Y, and appear in the innermost halo region and in the disk. (viii) From the result of the clean separations of the clusters into three groups, a three dimensional classification with three parameters, Z, Y and age is prsented. (ix) The anomalous C-M diagrams can be expalined in terms of the three principal parameters. That is, the anomaly of NGC 362 and NGC 7006 is accounted for by the smaller age of the order of $1{\sim}2{\times}10^9$ years rather than by the helium abundance difference, compared with M 3. (x) The difference in two Oosterhoff types I and II can be explained in terms of the mean mass difference of RR Lyrae variables rather than in terms of the helium abundance difference as suggested by Stobie. The mean mass of the variables in Oosterhoff type I clusters is smaller by $0.074m_{\odot}$ which is exactly consistent with Rood's estimate. Since it was found that the mean mass of RR Lyrae stars increases with decreasing Z, the two Oosterhoff types can be explained substantially by the metal abundance difference; the type II has Z<$3.4{\times}10^{-4}$, and the type I has higher Z than the type II.

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Ginsenosides의 처리온도(處理溫度) 및 시간(時閭)에 따른 반응속도론적(反應速度論的) 연구(硏究) (Kinetic Studies on the Thermal Degradation of Ginsenosides in Ginseng Extract)

  • 최진호;김두하;성현순;김우정;오성기
    • 한국식품과학회지
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    • 제14권3호
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    • pp.197-202
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    • 1982
  • 인삼제품제조용(人蔘製品製造用)엑기스의 숙성중(熟成中)에 일어나는 ginsenosides의 분해(分解)에 미치는 온도(溫度)의 영향(影響)을 구명(究明)하기 위하여 숙성온도(熟成溫度) 및 시간(時間)에 따른 ginsenosides의 함량변화(含量變化)로써 분해속도상수(分解速度常數) 및 반감기(牛減期)를 구(求)하였고 분해속도상수(分解速度常數)와 온도(溫度)에 대(對)한 Arrhenius plot에 의하여 활성화(活性化)에너지 및 $Q_{10}$ value를 구(求)하여 ginsenosides의 분해속도상수(分解速度常數)의 온도의존성(溫度依存性)에 대(對)한 관계식(關係式)을 설정(設定)하였다. 가. ginsenosides의 분해반응(分解反應)은 1차반응(次反應)을 나타냈으며 분해시(分解時)의 반감기(半減期)가 $100^{\circ}C$에서 34시간(時間), $90^{\circ}C$에서 70시간(時間), $80^{\circ}C$에서는 131시간(時間)이므로 ginsenosides의 함량변화(含量變化)만을 고려(考慮)한다면 $80^{\circ}C$이하(以下)의$70^{\circ}C$ 부근에서 숙성(熟成)함이 바람직하다. 나. 숙성중(熟成中)에 ginsenoside-Re가 감소(減少)하는 대신 $ginsenoside-Rg_2$가 증가(增加)하고 $ginsenoside-Rg_1$이 감소(減少)하는 대신 $ginsenoside-Rh_1$이 증가(增加)하므로 ginsenosides의 상호변환관계(相互變換關係)가 인정(認定)되었다. 다. ginsenosides의 분해시(分解時)의 온도상화(速度常數)가 $80^{\circ}C$에서 $5.30{\times}10^{-3}\;hr^{-1}$, $90^{\circ}C$에서 $9.90{\times}10^{-3}\;hr^{-1}$, 100"C에서는 $20.50{\times}10^{-3}\;hr^{-1}$으로서 숙성온도(熟成溫度)가 $10^{\circ}C$높아질 때마다 분해속도상수(分解速度常數)가 약(約) 2배(培) 증가(增加)하였고 또 $Q_{10}$ value도 $2.01{\sim}3.49$로서 숙성온도(熟成溫度)가 높아질수록 ginsenosides는 상대적(相對的)으로 불안정(不安定)하였다. 라. ginsenosides분해시(分解時)의 활성화(活性化)에너지 ($E_a$)는 $16.8{\sim}30.1$ kcal/mole의 범위 안에 있으며 ginsenoside-Re 및 $-Rg_1$$ginsenoside-Rb_1,\;-Rb_2$, -Rc 및 -Rd 보다 훨씬 높으므로 troil saponin이 diol saponin보다 온도(溫度)의 영향(影響)을 더 많이 받고 있었다. 마. total ginsenosides의 분해반응시(分解反應時)의 활성화(活性化)에너지($E_a$)는 17.7kcal/mole이었고 분해속도상수(分解速度常數)의 온도의존성(溫度依存性)은 $k=4.574{\times}10^8{\exp}(-8898.8/T)$의 관계식(關係式)으로 표시(表示)할 수 있다

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APR1400 증기발생기 습분분리기 진동 특성에 관한 연구 (A Study on Vibration Characteristics of Moisture Separator for APR1400 Steam Generator)

  • 조민기;박태정;하창훈;박누가
    • 한국소음진동공학회:학술대회논문집
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    • 한국소음진동공학회 2014년도 추계학술대회 논문집
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    • pp.99-101
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    • 2014
  • A Comprehensive Vibration Assessment Program (CVAP) for steam generator internals (SGI) of Advanced Power Reactor 1400 (APR1400) is being performed in accordance with the United States Nuclear Regulatory Commission (U.S. NRC) Regulatory Guide 1.20 (RG 1.20) revision 3. This paper studies the vibration characteristics of moisture separator assembly as part of the vibration and stress analysis program for APR1400 SGI CVAP. The natural frequencies, mode shapes, and structural behavior of moisture separator assembly were investigated through modal analysis using finite element method and experimental measurement. Since the moisture separator consists of several items with complicated shape, an idealized shell model was used in the finite element analysis. Group of local modes caused by moisture separators and significant modes of shroud and separator support plate were identified. The results of this paper are to be utilized in the structural response analysis of moisture separator assembly.

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전통장류 유래 GB-07균주에 의해 생산된 Compound K 함유 발효인삼의 항산화 및 항염증 활성 연구 (Study on antioxidant and anti-inflammatory activity of compound K extract produced by Saccharomyces servazzii(GB-07) strain derived from traditional soy)

  • 신동규;서정훈;조상민;최학주
    • 한국산학기술학회논문지
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    • 제18권9호
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    • pp.127-135
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    • 2017
  • 인삼은 약용으로 수 천년 동안 소중하게 사용되어 왔으며 근대에 들어와 단일 성분의 분리와 각 성분에 대한 효능의 과학적인 연구가 활발히 이어져 오고 있다. 최근에는 인삼 성분 중에 Rg3, compound K 등이 주목 받고 있으며 기능성을 갖는 다양한 소재들이 연구 개발되어 제품화 되고 있다. 본 연구에서는 전통 장류에서 유래한 효모균 (Saccharomyces servazzii, GB-07)을 이용하여 인삼 추출물을 발효하여 compound K를 생산하고 기능성을 확인하여 상업적 이용 가능성을 확인하였다. Compound K 함유 ($20{\mu}g/g$ 함유) 인삼 발효 추출물을 이용하여 항산화 및 항염증 활성을 측정하였다. 발효 인삼추출물은 자유 라디칼 DPPH (di(phenyl) -(2,4,6-trinitrophenyl)를 농도 의존적으로 소거하였으며. macrophage(RAW 264.7 cell)에서 ROS의 생성을 농도 의존적으로 억제하였다. 또한 LPS에 의해 유도된 염증 사이토카인 $IL-1{\beta}$와 IL-6 그리고 $TNF-{\alpha}$의 생성을 억제하는 것으로 나타났다. 본 연구 결과는 향후 공정개발과 다양한 효능 시험을 통해 compound K를 함유한 제품의 개발을 통한 산업화의 가능성 시사하고 있다.