Browse > Article

Preparation of Black Ginseng and its Antitumor Activity  

Lee, Jee-Hyun (College of Pharmacy, Chungnam National University)
Shen, Gui-Nan (College of Pharmacy, Chungnam National UniversityCollege of Pharmacy, Chungnam National University)
Kim, Eui-Keom (College of Pharmacy, Chungnam National University)
Shin, Hyun-Joong (Geum-San Chun-Yun Red Ginseng Co. Ltd.)
Myung, Chang-Seon (College of Pharmacy, Chungnam National University)
Oh, Han-Jin (Cheil General Hospital & Women's Health Care Center, Sungkyunkwan University)
Kim, Dong-Hee (Department of Pathology, College of Oriental Medicine, Daejon University)
Roh, Seong-Soo (Department of Herbalogy, College of Oriental Medicine, Daejon University)
Cho, Won (Department of Herbalogy, College of Oriental Medicine, Daejon University)
Seo, Young-Bae (Department of Herbalogy, College of Oriental Medicine, Daejon University)
Park, Yong-Jin (Baeknam Oriental Medical Hospital)
Kang, Cheol-Woo (Baeknam Oriental Medical Hospital)
Song, Gyu-Yong (College of Pharmacy, Chungnam National University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.4, 2006 , pp. 951-956 More about this Journal
Abstract
The purpose of this study is to prepare black ginseng and evaluate its antitumor activity. In order to achieve such aim, 5 year fresh ginsenges were steamed at 95'E for 3 hr in pottery apparatus and dried at $60^{\circ}C$ for 12-36 hr. This process was repeated again nine times in same condition. Among the ginseng saponins in black ginseng, the amount of Ginsenoside $Rg_3$ was examined by HPLC. 10.05 mE of Ginsenoside $Rg_3$ was obtained from 1 g of dried black ginseng prepared. The extract of black ginseng exhibited stronger cytotoxic activity against MCF-1, HT-1080 and Hepa 1C1C7 tumor cell lines in vitro than the extract of red ginseng. Also, the extract of black ginseng exhibited stronger antitumor activity(33%) in BDFl mice bearing Lewis lung carcinoma cells(LLC) than the extract of red ginseng(23%). From these results, it was concluded that Black ginseng had antitumor activity suggesting its application for the prevention and treatment of cancer.
Keywords
Black ginseng; Ginsenoside $Rg_3$; antitumor activity;
Citations & Related Records
연도 인용수 순위
  • Reference
1 이시진, 본초강목, 중국의학대계 41책, 서울, 정담, pp 231- 232, 1998
2 서성옥, 정철헌, 소화기계암의 수술후 면역기능에 대한 고려홍삼의 효과, Korea J. Ginseng Sci, 22(1):32-42, 1998
3 Okamura, N., Kobayashi, K., Akaike, A., Yagi, A. Protective effect of ginseng saponins against impaired brain growth in neonatal rats exposed to ethanol. Biol Pharm Bull, 17(2):270-274, 1994   DOI   ScienceOn
4 Kim, H.S., Lee, E.H., Ko, S.R., Choi, K.J., Park, J.H., Im, D.S. Effects of ginsenosides Rg3 and Rh2 on the proliferation of prostate cancer cells. 27(4):429-435, 2004   DOI
5 Cha, H.Y., Park, J.H., Hong, J.T., Yoo, H.S., Song, S., Hwang, B.Y., Eun, J.S., Oh, K.W. Anxiolytic-like effects of ginsenosides on the elevated plus-maze model in mice. 28(9):1621-1625, 2005   DOI   ScienceOn
6 Saito, H. et al. Prooc. 2nd International Ginseng symposium, 109, 1978
7 최영득, 박진아, 최형기, 남기열, 토끼와 흰쥐 음경해면체 이완작용에 홍삼사포닌 분획별 효과, Korea J. Ginseng Sci, 23(1):13-20, 1999
8 Keum, Y.S., Park, K.K., Lee, J.M., Chun ,K.S., Park, J.H., Lee, S.K., Kwon, H., Surh, Y.J. Antioxidant and anti-tumor promoting activities of the methanol extract of heat-processed ginseng, Cancer Lett. 150(1):41-48, 2000   DOI   ScienceOn
9 오미숙, 백기주, 전성실, 김규원, 최강주, 김남득, 홍삼 사포닌에 의한 유선상피 및 유선암세포의 분화 유도 효과 연구, Korea J. Ginseng Sci, 24(4):188-195, 2000
10 Rubinstein, L.V., Paull, K.D., Shoemaker, R.H., Simmon, R.M., Skehan, P. and Boyd, M.R. Correlation of screening data generated with a tetrazolium assay (MTT) versus a protein assay (SRB) against a broad panel of human tumor cell lines, Proceedings of the American Association for Cancer Research, 30, p 2418, 1989
11 김기환, 정인성, 홍삼다당체의 항암면역증강작용 연구, Korea J. Ginseng Sci, 21(2):78-84, 1997
12 제4회 국제인삼심포지움, 1984
13 Kim, Y., Kim, S.B., You, Y.J., Ahn, B.Z. Deoxypodophyllotoxin; the cytotoxic and antiangiogenic component from Pulsatilla koreana, Planta Med., 68(3):271-274, 2003
14 이혜연, 이한수, 홍삼 추출물의 임파구 증식 및 활성 촉진효과, Korea J. Ginseng Sci, 22(1):60-65, 1998
15 Kim, J.H., Lee, J.H., Jeong, S.M., Lee, B.H., Yoon, I.S., Lee, J.H., Choi, S.H., Kim, D.H., Park, T.K., Kim, B.K., Nah, S.Y. Stereospecific effects of ginsenoside Rg3 epimers on swine coronary artery contractions. 29(2):365-370, 2006   DOI   ScienceOn