Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Preparation of Black Ginseng and its Antitumor Activity

흑삼의 제조 및 항암효과

  • Lee, Jee-Hyun (College of Pharmacy, Chungnam National University) ;
  • Shen, Gui-Nan (College of Pharmacy, Chungnam National UniversityCollege of Pharmacy, Chungnam National University) ;
  • Kim, Eui-Keom (College of Pharmacy, Chungnam National University) ;
  • Shin, Hyun-Joong (Geum-San Chun-Yun Red Ginseng Co. Ltd.) ;
  • Myung, Chang-Seon (College of Pharmacy, Chungnam National University) ;
  • Oh, Han-Jin (Cheil General Hospital & Women's Health Care Center, Sungkyunkwan University) ;
  • Kim, Dong-Hee (Department of Pathology, College of Oriental Medicine, Daejon University) ;
  • Roh, Seong-Soo (Department of Herbalogy, College of Oriental Medicine, Daejon University) ;
  • Cho, Won (Department of Herbalogy, College of Oriental Medicine, Daejon University) ;
  • Seo, Young-Bae (Department of Herbalogy, College of Oriental Medicine, Daejon University) ;
  • Park, Yong-Jin (Baeknam Oriental Medical Hospital) ;
  • Kang, Cheol-Woo (Baeknam Oriental Medical Hospital) ;
  • Song, Gyu-Yong (College of Pharmacy, Chungnam National University)
  • 이지현 (충남대학교 약학대학) ;
  • 신귀남 (충남대학교 약학대학) ;
  • 김의검 (충남대학교 약학대학) ;
  • 신현중 ((주)금산천년홍삼) ;
  • 명창선 (충남대학교 약학대학) ;
  • 오한진 (성균관대학교 의과대학) ;
  • 김동희 (대전대학교 한의과대학 병리학교실) ;
  • 노성수 (대전대학교 한의과대학 본초학교실) ;
  • 조원 (대전대학교 한의과대학 본초학교실) ;
  • 서영배 (대전대학교 한의과대학 본초학교실) ;
  • 박용진 (백남한의원) ;
  • 강철우 (백남한의원) ;
  • 송규용 (충남대학교 약학대학)
  • Published : 2006.08.25
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Abstract

The purpose of this study is to prepare black ginseng and evaluate its antitumor activity. In order to achieve such aim, 5 year fresh ginsenges were steamed at 95'E for 3 hr in pottery apparatus and dried at $60^{\circ}C$ for 12-36 hr. This process was repeated again nine times in same condition. Among the ginseng saponins in black ginseng, the amount of Ginsenoside $Rg_3$ was examined by HPLC. 10.05 mE of Ginsenoside $Rg_3$ was obtained from 1 g of dried black ginseng prepared. The extract of black ginseng exhibited stronger cytotoxic activity against MCF-1, HT-1080 and Hepa 1C1C7 tumor cell lines in vitro than the extract of red ginseng. Also, the extract of black ginseng exhibited stronger antitumor activity(33%) in BDFl mice bearing Lewis lung carcinoma cells(LLC) than the extract of red ginseng(23%). From these results, it was concluded that Black ginseng had antitumor activity suggesting its application for the prevention and treatment of cancer.

Keywords

References

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