• Membrane and Water Treatment
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• 제8권3호
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• pp.293-310
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• 2017

An ultrasonic assisting vacuum membrane distillation (VMD) system was designed to promote the heat and mass transfer in membrane distillation (MD) process. Both the effects of operating conditions and ultrasonic parameters to permeation flux in this process were investigated; the heat and mass transfer mechanism was also being discussed in this paper. The results showed that the performance of VMD process was improved significantly by ultrasonic assisting. The permeation flux was boosted at a certain feed solution temperature, pressure at permeate side and feed solution velocity whether or not to PP and PTFE. The results also indicated that ultrasonic power and frequency also was the key factor affecting the mass and transfer efficiencies. The feed side transfer coefficient ($K_f$), corresponding to ultrasonic power ($K_f=4.406-0.026{\times}P+7.824{\times}10^{-5}{\times}P^2$) and ultrasonic frequency ($K_f=0.941+0.598{\times}f-0.012{\times}f^2+6.283{\times}10^{-5}f^3$), was obtained and employed in the modeling of ultrasonic assisting VMD process. The modeling results showed that the calculated value of $K_f$ aligned with experimental results well. Both variations of temperature polarization coefficient (TPC) and concentration polarization coefficient (CPC) were studied based on the obtained data. The results showed that both TPC and CPC were improved obviously by the ultrasonic parameters.

• Journal of Plant Biotechnology
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• 제47권3호
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• pp.194-202
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• 2020

The sweetpotatoes (Ipomoea batatas) generate adventitious roots (ARs) from cut stems that develop into storage roots and make for an important means of propagation. However, few studies have investigated the hormones involved in AR development in sweetpotato. In this study, the expression patterns of hormone-related genes involved in AR formation were identified using the transcriptome data. RNA-seq data from stems grown for 0 and 3 days after cutting were analyzed. In addition, hormone-related genes were identified among differentially expressed genes (DEGs) and filtered genes, and cluster analysis was used to characterize expression patterns by function. Most hormone-related regulated genes expressed 3 days after growing the cut stems were abscisic acid (ABA)-related genes, followed by ethylene- and auxin-related genes. For ABA, the biosynthesis genes (including genes annotated to NINE-CIS-EPOXYCAROTENOID DIOXYGENASE 3 (NCED3)) and signal transduction and perception genes (including genes annotated to PROTEIN PHOSPHATASE 2Cs (PP2Cs)) tended to decrease. Expression patterns of auxin- and ethylene-related genes differed by function. These results suggest that ABA, auxin, and ethylene genes are involved in AR formation and that they may be regulated in a hormone function-dependent manner. These results contribute to the identification of hormone functions during AR formation and may contribute to understanding the mechanism of AR formation in the sweetpotato.

• BMB Reports
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• 제46권8호
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• pp.410-415
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• 2013

Adhesion molecules such as ICAM-1 are important in the infiltration of leukocytes into the site of inflammation. In this study, we investigated the inhibitory effects of curcumin on ICAM-1 expression and monocyte adhesiveness as well as its underlying action mechanism in the TNF-${\alpha}$-stimulated keratinocytes. Curcumin induced expression of heme oxygenase-1 (HO-1) in the human keratinocyte cell line HaCaT. In addition, curcumin induced Nrf2 activation in dose- and time-dependent manners in the HaCaT cells. Curcumin suppressed TNF-${\alpha}$-induced ICAM-1 expression and subsequent monocyte adhesion, which were reversed by the addition of tin protoporphyrin IX (SnPP), a specific inhibitor of HO-1, or HO-1 knockdown using siRNA. Furthermore, Nrf2 knockdown using siRNA reversed the inhibitory effect of curcumin on the TNF-${\alpha}$-induced ICAM-1 expression and adhesion of monocytes to keratinocytes. These results suggest that curcumin may exert its anti-inflammatory activity by suppressing the TNF-${\alpha}$-induced ICAM-1 expression and subsequent monocyte adhesion via expression of HO-1 in the keratinocytes.

• JSTS:Journal of Semiconductor Technology and Science
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• 제14권4호
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• pp.495-502
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• 2014

Fast recovery diodes (FRDs) were developed using the $p^{{+}{+}}/n^-/n^{{+}{+}}$ epitaxial layers grown by low temperature epitaxy technology. We investigated the effect of electrostatic discharge (ESD) stresses on their electrical and switching properties using current-voltage (I-V) and reverse recovery time analyses. The FRDs presented a high breakdown voltage, >450 V, and a low reverse leakage current, < $10^{-9}$ A. From the temperature dependence of thermal activation energy, the reverse leakage current was dominated by thermal generation-recombination and diffusion, respectively, at low and high temperature regions. By virtue of the abrupt junction and the Pt drive-in for the controlling of carrier lifetime, the soft reverse recovery behavior could be obtained along with a well-controlled reverse recovery time of 21.12 ns. The FRDs exhibited excellent ESD robustness with negligible degradations in the I-V and the reverse recovery characteristics up to ${\pm}5.5$ kV of HBM and ${\pm}3.5$ kV of IEC61000-4-2 shocks. Likewise, transmission line pulse (TLP) analysis reveals that the FRDs can handle the maximum peak pulse current, $I_{pp,max}$, up to 30 A in the forward mode and down to - 24 A in the reverse mode. The robust ESD property can improve the long term reliability of various power applications such as automobile and switching mode power supply.

• 콘크리트학회논문집
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• 제24권2호
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• pp.173-183
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• 2012

최근 고강도 콘크리트의 폭렬현상에 관한 메커니즘의 연구와 더불어 폭렬을 방지하는 방법으로써 섬유혼입에 의한 콘크리트의 수증기압을 낮추는 방법이 선호되고 있는 실정이다. 주로 단일 형태의 폴리프로필렌 섬유를 혼입하여 폭렬을 저감하는 방법들이 사용하고 있으나 초고강도 콘크리트 영역에서는 실제로 급격하게 온도가 상승하는 경우에 있어서 폭렬 및 급격한 수증기 팽창압을 고려할 수 없다는 점을 들 수 있다. 따라서 이 연구에서는 콘크리트 내부온도상승조건에 따라 섬유의 용융점에 따른 공극의 형성 및 폭렬의 상관성을 분석하고자 하였으며, W/B 12.5%의 초고강도 콘크리트를 대상으로 용융점이 다른 PE섬유, PP섬유, 나일론섬유를 각각 0.15vol%, 0.25vol% 혼입하여 폭렬 성상, 수증기압, 시차열 중량 분석, 해석적 검토를 행하였다. 실험 결과, 동일 섬유 혼입률 조건에서 섬유의 용융점이 낮더라도 초고강도 콘크리트에서는 섬유의 기화에 의한 섬유의 중량손실이 발생하지 않으면 초기 폭렬의 방지가 어렵고, 가열시간 10분 전후의 빠른 공극을 형성하는 섬유가 폭렬의 방지에 효과적인 것으로 나타났다.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 2017년도 춘계공동학술대회
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• pp.128-128
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• 2017

현재 식물공장에서 사용되고 있는 이식장치는 핀을 이용하여 육묘트레이 위에서 플러그묘를 뽑아 육묘트레이 또는 포트로 옮겨 심는 방식을 채택하고 있다. 이러한 이식 방식은 셀과 셀 사이에 있는 다른 묘의 잎을 파지함으로써 묘를 2개 이상 취출하는 현상이라든지 핀에 의한 잎 손상 등이 우려되고 있어 이에 대한 개선책이 필요하다. 본 연구는 플러그묘의 이식작업 시 잎 손상을 줄이면서 기계 적응성을 향상시킬 수 있는 이식시스템을 개발하기 위해 진공흡인을 이용한 이식 메커니즘에 대해 검토하였다. 플러그묘 이식시스템은 육묘트레이 셀을 X-Y로 옮기는 묘 이송부, 육묘트레이의 셀 하단으로부터 진공을 발생시켜 묘를 떨어뜨리는 진공 흡인부, 낙하되는 묘를 감지하는 센서와 블로워 및 공압 실린더로 구성된 진공 발생부, 혈공된 포트를 진공 발생부의 유도관으로 옮기는 포트 이송부 등으로 설계 제작하였다. 이식 메커니즘은 육묘트레이 하단부로부터 플러그묘를 1개씩 진공흡인하는 방식을 채택하였고, 이를 위해 상하 모두 개방된 72공 육묘트레이($L538{\times}W280{\times}H45mm$)를 윗부분(Ø35mm) 보다 아랫부분(Ø37mm)의 셀이 넓은 형상으로 PP재질의 육묘트레이를 사출금형 제작하였다. 묘 이송부에 장착된 플러그묘는 X축 방향(12개 셀)으로 이식작업이 이루어지고, Y축(6개 셀)으로 이동된 후 다시 동일한 방향으로 연속 작업이 가능하도록 제어프로그램을 구성하였다. 이식 원리는 진공 흡인부에 플러그묘가 이송되면 진공 발생부의 흡착패드가 위로 전진하여 진공을 발생시켜 묘를 흡인하고, 유도관 내에 부착된 광화이버센서에 의해 묘를 감지하여 블로워와 공압실린더를 제어함으로써 이식 공정이 끝나게 된다. 로메인상추의 플러그묘를 대상으로 진공흡인 시험을 실시한 결과 묘 손상없이 이식작업이 가능한 것으로 확인되었다.

• 대한기계학회논문집A
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• 제31권1호
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• pp.36-42
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• 2007

This paper presents mechanical design, fabrication and test of a biomimetic fish robot actuated by a unimorph piezoceramic actuator, LIPCA(Lightweight Piezo-Composite curved Actuator.) We have designed a linkage mechanism that can convert bending motion of the LIPCA into the caudal fin movement. This linkage system consists of a rack-pinion system and four-bar linkage. Four types of artificial caudal fins that resemble caudal fin shapes of ostraciiform subcarangiform, carangiform, and thunniform fish, respectively, are attached to the posterior part of the robotic fish. The swimming test under 300 $V_{pp}$ input with 0.6 Hz to 1.2 Hz frequency was conducted to investigate effect of tail beat frequency and shape of caudal fin on the swimming speed of the robotic fish. At the frequency of 0.9 Hz, the maximum swimming speeds of 1.632 cm/s, 1.776 cm/s, 1.612 cm/s and 1.51 cm/s were reached for fish robots with ostraciiform, subcarangiform carangiform and thunniform caudal fins, respectively. The Strouhal number, which means the ratio between unsteady force and inertia force, or a measure of thrust efficiency, was calculated in order to examine thrust performance of the present biomimetic fish robot. The calculated Strouhal numbers show that the present robotic fish does not fall into the performance range of a fast swimming robot.

• The Korean Journal of Physiology and Pharmacology
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• 제21권2호
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• pp.259-265
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• 2017

Excessive influx and the subsequent rapid cytosolic elevation of $Ca^{2+}$ in neurons is the major cause to induce hyperexcitability and irreversible cell damage although it is an essential ion for cellular signalings. Therefore, most neurons exhibit several cellular mechanisms to homeostatically regulate cytosolic $Ca^{2+}$ level in normal as well as pathological conditions. Delayed rectifier $K^+$ channels ($I_{DR}$ channels) play a role to suppress membrane excitability by inducing $K^+$ outflow in various conditions, indicating their potential role in preventing pathogenic conditions and cell damage under $Ca^{2+}$-mediated excitotoxic conditions. In the present study, we electrophysiologically evaluated the response of $I_{DR}$ channels to hyperexcitable conditions induced by high $Ca^{2+}$ pretreatment (3.6 mM, for 24 hours) in cultured hippocampal neurons. In results, high $Ca^{2+}$-treatment significantly increased the amplitude of $I_{DR}$ without changes of gating kinetics. Nimodipine but not APV blocked $Ca^{2+}$-induced $I_{DR}$ enhancement, confirming that the change of $I_{DR}$ might be targeted by $Ca^{2+}$ influx through voltage-dependent $Ca^{2+}$ channels (VDCCs) rather than NMDA receptors (NMDARs). The VDCC-mediated $I_{DR}$ enhancement was not affected by either $Ca^{2+}$-induced $Ca^{2+}$ release (CICR) or small conductance $Ca^{2+}$-activated $K^+$ channels (SK channels). Furthermore, PP2 but not H89 completely abolished $I_{DR}$ enhancement under high $Ca^{2+}$ condition, indicating that the activation of Src family tyrosine kinases (SFKs) is required for $Ca^{2+}$-mediated $I_{DR}$ enhancement. Thus, SFKs may be sensitive to excessive $Ca^{2+}$ influx through VDCCs and enhance $I_{DR}$ to activate a neuroprotective mechanism against $Ca^{2+}$-mediated hyperexcitability in neurons.

• 대한한방부인과학회지
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• 제30권2호
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• pp.49-70
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• 2017

목 적: 본 연구는 한의학에서 면포질환에 빈번히 사용하는 대표적인 복합처방인 청상방풍탕의 열수 추출물 동결건조물의 피부 노화 개선 효과 평가를 위하여 피부재생, 주름개선, 미백 및 보습 효과를 각각 평가하였고, 기본적인 독성 평가의 일환으로 세포독성 또한 in vitro 방법으로 평가하였다. 방 법: 본 연구에서는 human normal fibroblast(CRL-2076) 세포 및 B16/F10 murine melanoma(CRL-6475) 세포에 대한 청상방풍탕 열수 추출 동결건조물(수율 18.71%)의 세포독성을 MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium Bromide) 방법을 통해 평가하였고, B16/F10 melanoma cells의 melanin 생성 억제 정도 및 melanin 합성 필수 효소인 tyrosinase 활성 억제와 arbutin을 비교 평가하여 미백효과를 평가하였다. 또한 피부 재생 및 주름 개선 효과를 transforming growth factor(TGF)-${\beta}1$와 fibroblast의 collagen type I 합성능을 비교, phosphoramidon disodium salt(PP)와 세포외 기질의 분해에 관여하는 elastase활성 억제를 비교, oleanolic acid(OA)와 hyaluronidase, collagenase 및 matrix metalloproteinase(MMP)-1 활성 억제를 비교하여 각각 평가하였고, 마우스 피부 수분함량 변화를 관찰하여 보습효과를 평가하였다. 결 과: 본 실험의 결과, 청상방풍탕은 human normal fibroblast 세포 및 B16/F10 murine melanoma 세포에서 최고 농도인 500 mg/ml까지 유의한 세포독성이 나타나지 않았고, fibroblast의 collagen type I 합성을 증가시켰다. 또한 세포외기질 파괴와 연관된 것으로 알려진 hyaluronidase, elastase, collagenase 및 MMP-1 활성을 각각 억제하였고 melanin의 생성에 관여하는 tyrosinase의 활성 및 B16/F10 melanoma 세포의 melanin 생성을 농도 의존적으로 차단함이 관찰되었다. 이와 더불어 정상매체 대조군에 비해 청상방풍탕 경구 투여군이 투여 용량 의존적으로 마우스 피부 수분 함량을 유의성 있게 증가시켰다. 결 론: 이상의 결과에서, 청상방풍탕은 세포 독성 없이 피부재생, 주름개선, 미백 및 보습 효과를 나타남이 관찰되어 차후 피부 개선 소재로서 그 가치가 높을 것으로 판단되나 금후 개별 구성 약재 각각에 대한 효능 및 생리활성을 나타내는 화학성분의 검색과 더불어 다양한 방면으로 기전적인 연구와 피부 보호 효과에 대한 in vivo평가를 체계적으로 수행해야 할 것으로 판단된다.

• The Korean Journal of Physiology and Pharmacology
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• 제11권2호
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• pp.65-70
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• 2007

Chinese herb medicines have traditionally been used to treat or alleviate the symptom of various diseases. The rationale for use of certain herbs to certain disorder is now getting unveiled by modern technology. In the present study, we investigated whether herb mix extract(HMX), which is alleged to be useful for gastric ulcer, protects stomach from oxidative stress. Rats were allowed to normal diet with and without HMX (1, 5, 10 mg/kg) for 30 days. To induce gastric ulcer, ethanol (75%, 1.5 ml) or acidified aspirin (100 mg/kg in 0.2 N HCl) was administered by oral route in 24 h-fasted rats and examined the gastric ulceration(bleeding) by measuring the size 1 h after the treatment. Results indicated the area of gastric bleeding was significantly less in HMX fed rats than in normal diet fed ones, and it was dependent on the duration and amount of HMX. To investigate the underlying mechanism by which HMX protects stomach from oxidative stress, expression of enzymes like heme oxygenase (HO), cyclooxygenase (COX), and inducible nitric oxide (iNOS) were investigated in MKN-74 cells, where aspirin or H. pylori was introduced. The results were compared with RAW 264.7 cells to check if there's cell specificities exist. The expression of HO-1 but not COX-2, iNOS was significantly increased by HMX. Furthermore, HO-1 inhibitor, SnPP IX reduced the HO-1 activity and reversed the survival rate in HMX-treated MKN-74 cells. There's no difference between RAW 264.7 cells and MKN-74 cells. We, thus, concluded that HMX is beneficial for protection from oxidative injury, and induction of HO-1 by HMX in gastric cells is, at least, responsible for protection from oxidative stress such as ethanol, aspirin and possibly H. pylori infection.