Browse > Article
http://dx.doi.org/10.15204/jkobgy.2017.30.2.049

Skin Regeneration, Anti-wrinkle, Whitening and Moisturizing Effects of Cheongsangbangpung-tang Aqueous Extracts with Cytotoxicity  

Woo, Chang-Yoon (Dept. of Korean Obstetrics & Gynecology, College of Korean Medicine, Daegu Haany University)
Kim, Dong-Chul (Dept. of Korean Obstetrics & Gynecology, College of Korean Medicine, Daegu Haany University)
Publication Information
The Journal of Korean Obstetrics and Gynecology / v.30, no.2, 2017 , pp. 49-70 More about this Journal
Abstract
Objectives: The present study is to observe the skin-regeneration, anti-wrinkle, whitening and skin moisturizing effects of Cheongsangbangpung-tang (CSBPT) with cytotoxicity. Methods: In the present study, cytotoxicity of CSBPT lyophilized aqueous extracts (yield=18.71%) was experimented against human normal fibroblast cells and B16F10 murine melanoma cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide (MTT) assay, and skin regeneration and anti-wrinkle effects were also showed through the assay of collagen type I synthesis by an enzyme immunoassay (EIA) kit as comparing with transforming growth factor (TGF)-${\beta}1$, hyaluronidase, collagenase and matrix metalloproteinase (MMP)-1 inhibitory assays as comparing with oleanolic acid (OA), and elastase inhibitory effects as comparing with phosphoramidon disodium salt (PP). In addition, whitening effects of CSBPT were observed by tyrosinase inhibitory assay and melanin formation test in B16/F10 melanoma cells as comparing with arbutin, and skin moisturizing effects were measured through mouse skin water contents test, respectively. Results: No CSBPT treatment related cytotoxic effects were demonstrated against human normal fibroblast cells and B16/F10 murine melanoma cells. CSBPT concentration-dependent increased collagen type I synthesis at human normal fibroblast cells. It also effectively suspreessed hyaluronidase, collagenase, elastase and MMP-1 activities, which were enzymes that related to declining of ECM and formation of wrinkle. CSBPT supressed B16/F10 melanoma cells's melanin productions with tyrosinase activity, which was an enzyme connected with melanin formation, and dose-dependent and significant increases of skin water contents were detected in CSBPT treated mouse skin as compared with vehicle control skins. Conclusions: CSBPT showed favorable and enough skin regeneration, anti-wrinkle, whitening and skin moisturizing effects at least in a condition of this experiment. However, more detail mechanism and in vivo skin protective efficacy studies should be conducted in future with the screening of the biological active compounds in individual herbs of Cheongsangbangpung-tang.
Keywords
Cheongsangbangpung-tang; Skin Regeneration; Anti-wrinkle; Whitening; Moisturizing;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Nema NK, et al. Cucumis sativus fruit-potential antioxidant, anti-hyaluronidase, and anti-elastase agent. Arch Dermatol Res. 2011;303(4):247-52.   DOI
2 Heo JC, et al. Anti-oxidant and antitumor activities of crude extracts by Gastrodia elata blume. Korean J Food Presev. 2006;13(1):83-7.
3 Wünsch E, Heindrich HG. Zur quantitativen bestimmung der kollagenase. Fresenius' J Analy Chem. 1964;203:397-8.
4 Losso JN, et al. Inhibition of matrix metalloproteinase-1 activity by the soybean Bowman-Birk inhibitor. Biotechnol Lett. 2004;26(11):901-5.   DOI
5 Masamoto Y, et al. Mushroom tyrosinase inhibitory activity of esculetin isolated from seeds of Euphorbia lathyris L. Biosci Biotechnol Biochem 2003;67(3):631-4.   DOI
6 Hosoi J, et al. Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid. Cancer Res. 1985;45(4):1474-8.
7 Kim KH, et al. Anti-skin-aging benefits of exopolymers from Aureobasidium pullulans SM-2001. J Cosmet Sci. 2014;65(5):285-98.
8 Camargo FB Jr, Gaspar LR, Maia Campos PM. Skin moisturizing effects of panthenol-based formulations. J Cosmet Sci. 2011;62(4):361-70.
9 Kanlayavattanakul M, Rodchuea C, Lourith N. Moisturizing effect of alcohol-based hand rub containing okra polysaccharide. Int J Cosmet Sci. 2012;34(3):280-3.   DOI
10 Kang SJ, et al. Fermentation with Aquilariae Lignum enhances the anti-diabetic activity of green tea in type II diabetic db/db mouse. Nutrients. 2014;6(9):3536-71.   DOI
11 Seong EJ, et al. Treatment of Wrinkles with Oriental Medicine. The J of Korean Med Ophthalmology & Otolaryngology & Dermatology. 2008;21(3):184-99.
12 Levene A. Pathological factors influencing excision of tumours in the head and neck. Part I. Clin Otolaryngol Allied Sci. 1981;6(2):145-51.   DOI
13 Ludbrook J. Update: microcomputer statistics packages. A personal view. Clin Exp Pharmacol Physiol. 1997;24(3-4):294-6.   DOI
14 Gilchrest BA. Skin aging and photoaging. Dermatol Nurs. 1990;2(2):79-82.
15 Wiedow O, et al. Elafin: an elastase-specific inhibitor of human skin. Purification, characterization, and complete amino acid sequence. J Biol Chem. 1990;265(25):14791-5.
16 Jung HJ. A study on the Sanggocheunron of the Somoon. Master thesis. Wonkwang Univ. 2000.
17 Han JM et al. The Study on the Korean and Western Medical Literatures for Skin Aging wrinkle, hyperpigmentation, dry skin, facial flush. The J of Korean Med Ophthalmology & Otolaryngology & Dermatology. 2014;21(2):1-13.
18 Jin KS, et al. Regulatory effect of atopic allergic reaction by Chungsangbangpoong-tang. The J of Korean Medicine Pediatrics. 2004;18(2):61-76.
19 Maity N, et al. Exploring Tagetes erecta Linn flower for the elastase, hyaluronidase and MMP-1 inhibitory activity. J Ethnopharmacol. 2011;137(3):1300-5.   DOI
20 Chan YY, Kim KH, Cheah SH. Inhibitory effects of Sargassum polycystum on tyrosinase activity and melanin formation in B16F10 murine melanoma cells. J Ethnopharmacol. 2011;137(3):1183-8.   DOI
21 Parvez S, et al. Natural occurring tyrosinase inhibitors: mechanism and applications in skin health, cosmetic and agriculture industries. Phytother Res. 2007;21(9):805-16.   DOI
22 Imodawa G. Recent advances in characterizing biological mechanisms underlying UV-induced wrinkles: a pivotal role of fibroblast-derived elastase. Arch dermatol Res. 2008;300(Suppl 1):7-20.   DOI
23 Kim EJ, et al. Skin aging and photoaging alter fatty acids composition, including 11,14,17-eicosatrienoic acid, in the epidermis of human skin. The J of Korean Med Sci. 2010;25(6):980-3.   DOI
24 Cho EA, et al. Screening of anti-wrinkle resource from herbal medicinal extracts and stability test of its cosmetic products. Korean J Medicinal Crop Sci. 2011;19(2):126-35.   DOI
25 Kawaharajo K, et al. Effect of phosphoramidon on protection against corneal ulcer caused by elastase and protease from Pseudomonas aeruginosa. Jpn J Exp Med. 1982;52(5):271-2.
26 Parvez S, et al. Survey and mechanism of skin depigmenting and lightening agents. Phytother Res. 2006;20(11):921-34.   DOI
27 Jung HK, et al. Whitening and anti-wrinkle effects of Korean native Dendrobium moniliforme methanol extract. Korean J Medicinal Crop Sci. 2014;22(5):331-8.   DOI
28 Liao PL, et al. Anti-ageing effects of alpha-naphthoflavone on normal and UVB-irradiated human skin fibroblasts. Exp Dermatol. 2012;21(7):546-8.   DOI
29 Bernard E, Hornebeck W, Robert L. Elastase-type endopeptidase of fibroblasts. Effect of metalloprotease inhibitors. C R Acad Sci III. 1995;318(2):179-82.
30 Nishimori Y, et al. UVB-induced ultrastructural changes of collagen bundles in hairless mouse skin. J Dermatol Sci. 1997;15:125.
31 Jablonska E, et al. Transforming growth factor-${\beta}1$ induces expression of human coagulation factor XII via Smad3 and JNK signaling pathways in human lung fibroblasts. J Biol Chem. 2010; 285(15):11638-51.   DOI
32 O'Donoghue JL. Hydroquinone and its analogues in dermatology - a risk-benefit viewpoint. J Cosmet Dermatol. 2006;5(3):196-203.   DOI
33 Pop C, Vlase L, Tamas M. Natural resources containing arbutin. Determination of arbutin in the leaves of Bergenia crassifolia (L.) Fritsch. acclimated in Romania. Not Bot Hort Agrobot Cluj. 2009;37(1):129-32.
34 Assoian RK, et al. Transforming growth factor-beta in human platelets. Identification of a major storage site, purification, and characterization. J Biol Chem. 1983;258(11):7155-60.
35 Kitagishi K, Hiromi K. Binding between thermolysin and its specific inhibitor, phosphoramidon. J Biochem. 1984;95(2):529-34.   DOI
36 Tsatmali M, Ancans J, Thody AJ. Melanocyte function and its control by melanocortin peptides. J Histochem Cytochem. 2002;50(2):125-33.   DOI
37 Aguirre MC, et al. Topical anti-inflammatory activity of 2alpha-hydroxy pentacyclic triterpene acids from the leaves of Ugni molinae. Bioorg Med Chem. 2006;14(16):5673-7.   DOI
38 Juan ME, et al. Antiproliferative and apoptosis-inducing effects of maslinic and oleanolic acids, two pentacyclic triterpenes from olives, on HT-29 colon cancer cells. Br J Nutr. 2008;100(1):36-43.   DOI
39 Hearing VJ. Biogenesis of pigment granules: a sensitive way to regulate melanocyte function. J Dermatol Sci. 2005;37(1):3-14.   DOI
40 Kameyama K, et al. The expression of tyrosinase, tyrosinase-related proteins 1 and 2 (TRP1 and TRP2), the silver protein, and a melanogenic inhibitor in human melanoma cells of differing melanogenic activities. Pigment Cell Res. 1995;8(2):97-104.   DOI
41 Solano F, et al. Hypopigmenting agents: an updated review on biological, chemical and clinical aspects. Pigment Cell Res. 2006;19(6):550-71.   DOI
42 An BJ, et al. Antioxidant effects and application as natural ingredients of Korean Sanguisorbae officinalis L. J Korean Soc Appl Biol Chem. 2004;47(2):244-50.
43 Busca R, Ballotti R. Cyclic AMP a key messenger in the regulation of skin pigmentation. Pigment Cell Res. 2000;13(2):60-9.   DOI
44 Jacobi O. About the mechanism of moisture regulation in the horny layer of the skin. Proc Sci Sec Toilet Goods Assoc. 1959;31:22.
45 Kim JH, Yoo DY. A case report of acne vulgaris patient treated with Cheongsangbangpung-tang. The J of Daejeon Korean Medicine. 2013;22(1):185-91.
46 Kim HC, Oh KD. Review in the Effects Influencing Skin Aging and Its Prevention and Cure. Asian J of Beauty and Cosmetology. 2011;9(1):1-9.
47 Jung MS. Study on Middle-Aged Women's Understanding of Facial Skin Aging and Desire to Improve. Master thesis. Seokyung Univ. 2014.
48 Rhee SH. The clinical classification as identify of symptoms about 54 cases of patients with Acne. Korean J Korean Medical Institute of Dermatology and Aesthetics. 2005;1(1):114-26.
49 Seo EJ, Hwang CY, Kim NK. Experimental study of GagamCheongsang BangPung-Tang on the anti-inflammatory effects. The J of Korean Med Ophthalmology & Otolaryngology & Dermatology. 2008;21(2):54-70.
50 Kim YH, et al. Anti-wrinkle activity of ziyuglycoside I isolated from a Sanguisorba officinalis root extract and its application as a cosmeceutical ingredient. Biosci Biotechnol Biochem. 2008;72(2):303-11.   DOI