• Title/Summary/Keyword: 2-Loop Structure

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Sequencing and annotation of the complete mitochondrial genome of a threatened labeonine fish, Cirrhinus reba

  • Islam, Mohammad Nazrul;Sultana, Shirin;Alam, Md. Jobaidul
    • Genomics & Informatics
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    • v.18 no.3
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    • pp.32.1-32.7
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    • 2020
  • The mitochondrial genome of a species is an essential resource for its effective conservation and phylogenetic studies. In this article, we present sequencing and characterization of the complete mitochondrial genome of a threatened labeonine fish, Cirrhinus reba collected from Khulna region of Bangladesh. The complete mitochondrial genome was 16,597 bp in size, which formed a circular double-stranded DNA molecule containing a total of 37 mitochondrial genes (13 protein-coding genes, 2 ribosomal RNA genes, and 22 transfer RNA genes) with two non-coding regions, an origin of light strand replication (OL) and a displacement loop (D-loop), similar structure with other fishes of Teleostei. The phylogenetic tree demonstrated its close relationship with labeonine fishes. The complete mitogenome of Cirrhinus reba (GenBank no. MN862482) showed 99.96% identity to another haplotype of Cirrhinus reba (AP013325), followed by 90.18% identity with Labeo bata (AP011198).

A Study on the Position Control of an Electro-Hydraulic Servomechanism Using Variable Structure System (가변구조를 이용한 전기-유압서보계의 위치제어에 관한 연구)

  • 허준영;권기수;하석홍;조겸래;이진걸
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.13 no.2
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    • pp.213-220
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    • 1989
  • This paper describes the application of the variable structure control(VSC) concept for the position control of an electro-hydraulic servomotor system. The basic philosopy of VSC is that the structure of the feedback control is altered as the state crosses discontinuity surfaces in the state space with the result that certain desirable properties are achieved. The switching of the control function yields total(or selective) invariance to system parameter variations and disturbances, and closed loop eigen value placement in time-varing and uncertain systems. The control scheme is derived, implemented and tested in the laboratory where analog controller have been used to control the representive servosystem. The control system schematics are given and simple results are shown for illustration. And the results of variable structure system for the electro-hydraulic servomotor were compared to that of the fixed structure system when load disturbance and system parameter variation exists.

A Study on Indentification of Machine Tool Dynamics by Impulse Shock (충격력 에 의한 공작기계 동특성 규명 연구)

  • 신민재;이종원
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.7 no.2
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    • pp.138-144
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    • 1983
  • To evaluate the dynamic characteristics of machine tool system, the system is modelled as a closed-loop system composed of cutting process and improved machine tool structures. The proposed machine tool structure model is constructed in consideration of energy transfer through the system. A new methodology to identify the machine tool dynamics by adopting impulse response and impulse cutting techniques is also proposed. It is shown that the methodology is successfully applied to a machine tool system to identify its dynamic characteristics employing the improved model.

Nucleotide Sequence and Secondary Structure of 16S rRNA from Sphingomonas chungbukensis DJ77 (Sphingomonas chungbukensis DJ77의 16S rRNA 염기서열과 이차구조)

  • Lee Kwan-Young;Kwon Hae-Ryong;Lee Won-Ho;Kim Young-Chang
    • Korean Journal of Microbiology
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    • v.41 no.2
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    • pp.125-128
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    • 2005
  • A 16S ribosomal RNA gene from S. chungbukensis DJ77 has been sequenced. This sequence had a length of 1,502 bp and was extended for 29 bp at 5' and for 37 bp at 3' from the partial sequence (1,435 bp) registered in 2000 year. Besides, 1 bp was newly added near to the 3' end. We made the secondary structure of the 16S rRNA based on E. coli model and found four specific regions. We found constant and variable regions in genus Sphingomonas as the result of multiple alignment of 16S rRNA gene sequences from Sphingomonas spp. and S. chungbukensis DJ77. We found a stem loop structure in S. chungbukensis DJ77, which was only discovered in C. jejuni to date. It showed the structural agreement despite the difference of the sequences from the both organisms. Finally, S. chungbukensis DJ77 belonged to cluster II (Sphingobium) group, after the classification using phylogenetic analysis and nucleotide signature analysis.

Electrically Small Square Loop Antenna with SRR (Split Ring Resonator) Cover Structure (SRR (Split Ring Resonator) 덮개 구조를 갖는 전기적 소형 정사각형 루프 안테나)

  • Kim, Yong-Jin;Kim, Jung-Han;Lee, Hong-Min
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.45 no.6
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    • pp.52-58
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    • 2008
  • In this paper, electrically small square loop antenna with SRR (Split Ring Resonator) cover structure is built and tested. The proposed antenna has very small size, ka = 0.34 by Chu limit. The experimental result shows that the resonant frequency and impedance bandwidth($VSWR{\leq}2$) are 906MHz and 5.8MHz (901.7 - 907.5MHz), respectively. The proposed antenna is matched and designed by equivalent circuit model. The proposed antenna is fabricated simple structure without additional matching network and printed on a Teflon substrate without ground plane. Therefore, it has advantages of low cost, small size, and light weight and will be applied to wireless communication systems where small antennas are required.

Optimal design of switched reluctance motor using 2D FEM and 3D equivalent magnetic circuit network method (2차원 FEM과 3차원 등가자기회로방법을 이용한 SRM의 최적 설계)

  • Jung, S.I.;Kim, Y.H.;Lee, J.;Kim, H.L.
    • Proceedings of the KIEE Conference
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    • 2001.10a
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    • pp.125-127
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    • 2001
  • Switched reluctance motor (SRM) has some advantages such as low cost, high torque density etc. However SRM has inevitably high torque ripple due to the double salient structure. To apply SRM to industrial field, we have to minimize torque ripple, which is the weak-Point of SRM. This paper presents optimal design process of SRM using numerical method such as 2D finite element method (FEM) and 3D equivalent magnetic circuit network method (EMCNM). The electrical and geometrical design parameters have been adopted as 2D design variables. The overhang structure of rotor has been also adopted as 3D design variable. From this work, we can obtain the optimal design, which minimize the torque ripple and maximize energy conversion loop.

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Backbone assignment of HMGB1 A-box and molecular interaction with Hoxc9DBD studied by paramagnetic probe

  • Choi, Ji Woong;Park, Sung Jean
    • Journal of the Korean Magnetic Resonance Society
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    • v.25 no.2
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    • pp.17-23
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    • 2021
  • High mobility group protein B1 (HMGB1) is a highly conserved, non-histone, chromatin associated nuclear protein encoded by HMGB1 gene. HMGB1 proteins may be general co-factors in Hox-mediated transcriptional activation that facilitate the access of Hox proteins to specific DNA targets. It is unclear that the exact binding interface of Hoxc9DBD and HMGB1. To identify the interface and binding affinity of Hoxc9DBD and HMGB1 A-box, the paramagnetic probe, MTSL was used in NMR titration experiment. It is attached to the N-terminal end of HMGB1 A-box by reaction with thiol groups. The backbone assignment of HMGB1 A-box was achieved with 3D NMR techinques. The 15N-labeled HMGB1 A-box was titrated with MTSL-labeled Hoxc9DBD respectively. Based on the chemical shift changes we can identify the interacting residues and further map out the binding sites on the protein structure. The NMR titration result showed that the binding interface of HMGB1 A-box is around loop-1 between helix-1 and helix-2. In addition, the additional contacts were found in N- and C-terminus. The N-terminal arm region of Hoxc9DBD is the major binding region and the loop between helix1 and helix2 is the minor binding region.

2 DOF robust performance controller design for linear system with time delay and parameter uncertainty (시간지연 및 파라미터 불확실성을 갖는 선형 시스템의 2 자유도 견실성능 제어기 설계)

  • 이갑래;정은태;최봉렬;박홍배
    • Journal of the Korean Institute of Telematics and Electronics S
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    • v.34S no.1
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    • pp.43-53
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    • 1997
  • A robust stability condition for linear systems with time delay in all variables and parameter uncertainties in all system matrices is derived. Robust performance condition that accounts for robust model-matching of closed loop system and disturbance rejection is also derived. Using the robust performance condition, robust $H^{\infty}$ controller and .mu.(sgructured singular value) controller with two-degree-of-freedom(2DOF) are designed. The controller structure is considered for $H^{\infty}$ controller, while uncertainity structure is considered for .mu. controller. Using the proposed method, $H^{\infty}$ and .mu. controllers for underwater vehicle with time delay and parameter variations are designed. Simulations of a design example with hydrodynamic parameter variations and disturbance are presented to demonstrate the achievement of good robust performance.ce.

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Computational Tridimensional Protein Modeling of Cry1Ab19 Toxin from Bacillus thuringiensis BtX-2

  • Kashyap, S.;Singh, B.D.;Amla, D.V.
    • Journal of Microbiology and Biotechnology
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    • v.22 no.6
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    • pp.788-792
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    • 2012
  • We report the computational structural simulation of the Cry1Ab19 toxin molecule from B. thuringiensis BtX-2 based on the structure of Cry1Aa1 deduced by x-ray diffraction. Validation results showed that 93.5% of modeled residues are folded in a favorable orientation with a total energy Z-score of -8.32, and the constructed model has an RMSD of only $1.13{\AA}$. The major differences in the presented model are longer loop lengths and shortened sheet components. The overall result supports the hierarchical three-domain structural hypothesis of Cry toxins and will help in better understanding the structural variation within the Cry toxin family along with facilitating the design of domain-swapping experiments aimed at improving the toxicity of native toxins.

Variable structure control of AC servo motors for high performance (가변 구조 제어를 이용한 AC 서보 모터의 고성능 제어)

  • Kim, Jung-Ho;Eun, Yong-Soon;Cho, Dong-Il
    • Journal of Institute of Control, Robotics and Systems
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    • v.2 no.4
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    • pp.351-361
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    • 1996
  • A variable structure controller is developed for an AC servo motor used in CNC milling machines. The designed controller is implemented as an outer loop controller to a factory designed motor-servopack system. The robustness parameter is tuned for a fast response when the speed tracking error is large, while it is tuned for small oscillations when the speed tracking error is small. The designed controller is installed on a CNC machine using a PC. Cutting experiments show improved performance over the factory-designed controller.

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