• Design & Manufacturing
• /
• v.15 no.2
• /
• pp.11-16
• /
• 2021

Recently, three-dimensional (3D) cell culture systems, which are superior to conventional two-dimensional (2D) vascular systems that mimic the in vivo environment, are being actively studied to reproduce drug responses and cell differentiation in organisms. Conventional two-dimensional cell culture methods (scaffold-based and non-scaffold-based) have a limited cell growth rate because the culture cannot supply the culture medium as consistently as microvessels. To solve this problem, we would like to propose a 3D culture system with an environment similar to living cells by continuously supplying the culture medium to the bottom of the 3D cell support. The 3D culture system is a structure in which microvascular structures are combined under a scaffold (agar, collagen, etc.) where cells can settle and grow. First, we have manufactured molds for the formation of four types of microvessel-mimicking chips: width / height ①100 ㎛ / 100 ㎛, ②100 ㎛ / 50 ㎛, ③ 150 ㎛ / 100 ㎛, and ④ 200 ㎛ / 100 ㎛. By injection molding, four types of microfluidic chips were made with GPPS (general purpose polystyrene), and a 100㎛-thick PDMS (polydimethylsiloxane) film was attached to the top of each microfluidic chip. As a result of observing the flow of the culture medium in the microchannel, it was confirmed that when the aspect ratio (height/width) of the microchannel is 1.5 or more, the fluid flows from the inlet to the outlet without a backflow phenomenon. In addition, the culture efficiency experiments of colorectal cancer cells (SW490) were performed in a 3D culture system in which PDMS films with different pore diameters (1/25/45 ㎛) were combined on a microfluidic chip. As a result, it was found that the cell growth rate increased up to 1.3 times and the cell death rate decreased by 71% as a result of the 3D culture system having a hole membrane with a diameter of 10 ㎛ or more compared to the conventional commercial. Based on the results of this study, it is possible to expand and build various 3D cell culture systems that can maximize cell culture efficiency by cell type by adjusting the shape of the microchannel, the size of the film hole, and the flow rate of the inlet.

• Journal of the Korean Society of Manufacturing Process Engineers
• /
• v.15 no.2
• /
• pp.104-110
• /
• 2016

The process of three-dimensional (3D) printing (also known as "rapid prototyping" and "additive manufacturing") uses computer-created digital models to produce 3D objects with a desired shape by stacking materials through a layer-by-layer process. The industrial potential and feasibility of 3D printing technology were recently highlighted in President Obama's State of the Union address in 2013. Since his speech, worldwide investment in and attention toward 3D printing technology have increased explosively. In addition, a number of 3D printing technology-based start-up companies have been established and evaluated as emerging enterprises making successful business models. In this paper, successful start-up companies (domestic and overseas) based on 3D printing technology will be reviewed.

• Proceedings of the Korea Inteligent Information System Society Conference
• /
• 1999.10a
• /
• pp.313-320
• /
• 1999

In this paper, a translation, rotation and scale invariant system for the classification of closed 2D images using the bispectrum of a contour sequence and the weighted fuzzy mean method is derived and compared with the classification process using one of the competitive neural algorithm, called a LVQ(Learning Vector Quantization). The bispectrun based on third order cumulants is applied to the contour sequences of the images to extract fifteen feature vectors for each planar image. These bispectral feature vectors, which are invariant to shape translation, rotation and scale transformation, can be used to represent two-dimensional planar images and are fed into an classifier using weighted fuzzy mean method. The experimental processes with eight different shapes of aircraft images are presented to illustrate the high performance of the proposed classifier.

• Proceedings of the Computational Structural Engineering Institute Conference
• /
• 1994.04a
• /
• pp.137-144
• /
• 1994

In this study, a new procedure for solving 2-D dynamic problems of semi-infinite medium in time domain by boundary element method (BEM) is presented. Efficient modelling of the far field region, infinite boundary elements are introduced. The shape function of the infinite boundary element is a combination of decay functions and Laguerre functions. Though the present shape functions have been developed for the time domain analysis, they may be also applicable to the frequency domain analysis. Through the response analysis in a 2-D half space under a uniformly distributed dynamic load, it has been found that an excellent accuracy can be achieved compared with the analytical solution

• Journal of the Korean Society of Industry Convergence
• /
• v.14 no.2
• /
• pp.67-72
• /
• 2011

Recently, it is possible for small sized and high speed diesel engines by development of commonrail system. And in order to increase the engine performance, the cylinder firing pressure is a tendency which increases. On the other side, the weight of engine becomes lightly in spit of high performance diesel engine. Therefore, the weight optimization for engine components is very important point on the design process. Also, the weight optimization must necessarily be considered the robust design against a fatigue failure. This paper focuses on the weight optimization of crankshaft according to web shape at the light duty diesel engine, and torsion characteristics of crankshaft is considered with 1D and 3D analysis tools.

• Proceedings of the Korean Society for Technology of Plasticity Conference
• /
• 2004.05a
• /
• pp.66-69
• /
• 2004

A new concept of multi-point dieless elasto-forming method has been developed to make various shape of curved surface without conventional dies. The developed dieless elasto-forming system consists of discrete punches controlled by servo motors and various kinds of elastomers(rubber and foam). To predict optimal position of punch elements, DTF(deformation transfer function) was introduced, and FEM analysis was carried out. The optimal arrangement of elastomer was selected considering characteristics of each elastomer, and a desired concave shape was formed. The experimental results were consistent with the numerical ones.

• Structural Engineering and Mechanics
• /
• v.15 no.5
• /
• pp.535-550
• /
• 2003

In this paper, a boundary-type meshfree method, the boundary radial point interpolation method (BRPIM), is presented for solving boundary value problems of two-dimensional solid mechanics. In the BRPIM, the boundary of a problem domain is represented by a set of properly scattered nodes. A technique is proposed to construct shape functions using radial functions as basis functions. The shape functions so formulated are proven to possess both delta function property and partitions of unity property. Boundary conditions can be easily implemented as in the conventional Boundary Element Method (BEM). The Boundary Integral Equation (BIE) for 2-D elastostatics is discretized using the radial basis point interpolation. Some important parameters on the performance of the BRPIM are investigated thoroughly. Validity and efficiency of the present BRPIM are demonstrated through a number of numerical examples.

• Proceedings of the IEEK Conference
• /
• 2002.07c
• /
• pp.1523-1526
• /
• 2002

In this paper, we propose a method fur measuring the dimensions of an arbitrary object using geometric relationship between a perspective projection image and a rectangular parallelepiped model. For recognizing the vertexes of the rectangular parallelepiped surrounding an arbitrary object, the method adopts a strategy that derives the equations for vertex recognition from the geometrical relationships for image formation between 2D image and the rectangular parallelepiped model. extracts from 2D image with vertical view features (or junctions) of minimum quadrangle circumscribing an arbitrary shape object, and then recognizes vertexes from the features with the equations. Finally, the dimensions of the object are calculated from these results of vertex recognition. By the experimental results, it is demonstrated that this method is very effective to recognize the vertexes of the arbitrary objects.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.25 no.3B
• /
• pp.541-548
• /
• 2000

In the MPEG-4 standardization phase, many methods for coding the irregular shaped VOP (video object Plane) have been researched. Texture coding is one of interesting research items in the MPEG-4. There are the Low pass extrapolation (LPE) padding, the shape adaptive DCT (SA-DCT), and the Extension-Interpolation (EI)/2D-DCT proposed in [1] as texture coding methods. the EI/2D-DCT is the method extending and interpolating luminance values from and Arbitrarily Shaped (AS) image segment into an 8 x 8 block and transforming the extended and interpolated luminance values by the 8x8 DCT. although the EI/2D-DCT and the SA-DCT work well in coding the As image segments. they are degraded since they use one-dimensional (1-D) methods such as the 1D-EI and the 1D-DCT in the two-dimensional (2-D) space. in this paper, we analyze the performance of the EI/2D-DCTand propose a new non-symmetric sig-sag scanning method, which non-symmetrically scans the quantized coefficients in the DCT domain to improve the EI/2D-DCT.

• Computers and Concrete
• /
• v.26 no.6
• /
• pp.565-576
• /
• 2020

Experimental and discrete element methods were used to investigate the effects of angle of Y shape non-persistent joint on the tensile behaviour of joint's bridge area under brazilian test. concrete samples with diameter of 100 mm and thikness of 40 mm were prepared. Within the specimen, two Y shape non-persistent notches were provided. The large notch lengths were 6 cm, 4 cm and 2 cm. the small notch lengths were 3 cm, 2 cm and 1 cm. The angle of larger notch related to horizontal axis was 0°, 30°, 60°, 90°. Totally, 12 different configuration systems were prepared for Y shape non-persistent joints. Also, 18 models with different Y shape non-persistent notch angle and notch length were prepared in numerical model. The large notch lengths were 6 cm, 4 cm and 2 cm. the small notch lengths were 3 cm, 2 cm and 1 cm. The angle of larger notch related to horizontal axis was 0, 30, 60, 90, 120 and 150. Tensile strength of model materil was 1 MPa. The axial load was applied to the model by rate of 0.02 mm/sec. This testing showed that the failure process was mostly governed by the Y shape non-persistent joint angle and joint length. The tensile strengths of the specimens were related to the fracture pattern and failure mechanism of the discontinuities. It was shown that the tensile behaviour of discontinuities is related to the number of the induced tensile cracks which are increased by increasing the joint length and joint angle. The minimum tensile strength occurs when the angle of larger joint related to horizontal axis was 60°. Also, the maximum compressive strength occurs when the angle of larger joint related to horizontal axis was 90°. The tensile strength was decreased by increasing the notch length. The failure pattern and failure strength are similar in both methods i.e. the experimental testing and the numerical simulation methods.