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Effects of Dietary Supplementation of Mixed Probiotics on Production Performance and Intestinal Environment in Broiler Chicken (혼합 생균제의 사료 내 첨가 급여가 육계의 생산성과 장내 환경에 미치는 영향)

  • Oh, Seong Taek;Kang, Chang Won;Kim, Eun Jib
    • Korean Journal of Poultry Science
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    • v.41 no.2
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    • pp.143-149
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    • 2014
  • This study was conducted to investigate the effects of dietary supplementation of the mixture of probiotics (MP) on growth performance, size of small intestine, cecal microflora and ammonia concentrations in broiler chicks. A total of 700, one-day-old male broiler chicks were randomly allotted to four treatments with seven replications having 25 birds per pen. The birds were fed one of the four experimental diets; containing no antibiotics nor MP (negative control, NC), containing antibiotics without MP(positive control, PC), negative control with MP 0.1% and negative control with MP 0.2% for 5 weeks. During overall experiment, birds in PC and MP treatments had higher final BW and daily BW gains than birds in NC treatments; however, the significance was not identified. The feed conversion ratio of the chicks fed the diet containing MP was significantly improved as compared to those fed the NC diets. The weights of jejunum were increased by the MP (p<0.05), but weights of duodenum, ileum and length of small intestine were similar among the groups. Birds in PC treatment showed lower populations of total microbes and lactic acid bacteria than other groups (p<0.05), cecal ammonia concentrations of the chicks fed the diet containing MP were significantly decreased as compared to those of NC and PC (p<0.01). In conclusion, MP added to the broiler diets improved the feed conversion rate and reduced cecal ammonia concentration.

자동차 배출가스 규제 및 대책

  • 조병환
    • Journal of the korean Society of Automotive Engineers
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    • v.5 no.3
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    • pp.8-15
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    • 1983
  • 1960년초 이래 우리나라는 고도의 경제성장을 이룩하게 되었으며 이러한 경제성장에 따른 산업화 사회의 특징인 공업화 도시화 인구집중 현상은 우리나라에도 심각한 환경오염 문제를 야기시켜 왔다. 또한 같은 기간동안 자동차의 보유대수 역시 급격한 증가를 보여왔던바, 1962년 30,000 대에서 1982년도 자동차 등록대수는 약 640,000대로 증가하게 되었으며 대부분의 자동차가 대 도시에 집중되어 있다. 특히 전체 차량의 40%가 집중되어 있는 서울시의 경우에는 자동차 배 출가스로 인한 대기오염문제가 도시민의 건강과 재산에 많은 피해를 일으키게 되었다. 우리나 라는 선진 외국의 대도시에 비해 자동차의 1일 주행거리가 3배 내지 5배를 더 주행하고 있고 자동차 배출가스 기준의 완화, 노후차량의 증가, 도로율의 불황 및 도로조건의 불비 등은 도시 중심천의 자동차 집중현상과 함께 자동차 배출가스로 인한 대기오염 문제가 보다 더 심각하게 우려되었고, 따라서 자동차 배출가스규제 강화의 필연성이 계속해서 주장되어 왔다. 자동차로 인한 공해문제는 1940년도 후반 미국 남부 California 지방에서 광화학스모그의 발생과 함께 거 론되기 시작하였으나 본격적으로 문제가 제기된 것은 급격한 자동차 증가로 인한 도시민의 건 강피해가 발생하기 시작한 것은 1960년대이다. 처음으로 규제되기 시작한 것은 1965년 미국에서 "자동차 오염방지법"의 제정부터이며 1968년부터 자동차 배출가스에 대한 규제가 시작되었다. 한편 일본은 1973년, 유럽에서는 1975년부터 일산화탄소, 탄화수소, 질소산화물 및 매연에 대한 규제를 시작하게 되었다. 우리나라에서는 "도로운송차량법"에서 도로교통의 안전과 질서유지라는 측면에서 일부 규제하여 오다가 1977년말 "환경보전법"이 제정 공포되면서 1980년 1월 환경정의 발족과 함께 실제로 자동차 배출가스를 규제하게 되었다.발족과 함께 실제로 자동차 배출가스를 규제하게 되었다.을 SUP7H종으로 더욱 향상된 것이 실용 화되고 있다. 아래에서 이에 대한 기계적 특성을 중심으로 검토키로 한다.9%내인 0.07cm 정도였으나 1973년과 1974년의 방축년에는 조차 3개 처리구(3.4-5.18cm)에 필적되는 연평균 4.16cm로 나타났다. 5. 전체 시험구로부터으 연평균 토양유실량은 Sharpsburg 점질양토에 대한 S.C.S 한계허용치 10ton/ha/year 이내로 나타났다. 비처리구에서의 토양유실량은 평균 2.56ton/ha/year로 높게 나타난 반면 3개의 서로 다른 추리구인 비수구, 초생수로구 및 Bromegrass구에서는 각각 0.152, 0.192 및 0.290ton/ha/year로 낮은 결과를 가져왔다. 6. 평균 침전량에 대한 L.S.D. 검정 걸과 전시험구중 비처리구가 고도의 유의차를 나타낸 반면 비수구, 초생수로구 및 Bromegrass 목초구 간에는 아무런 유의차가 인정되지 않았다. 7. 농지보전 처리구인 배수구와 초생수로구는 비처리구에 비해 낮은 침두 유출량과 낮은 토양유실량을 나타내었다.구보다 14% 절감되는 것으로 나타났다.작용하는 것으로 사료된다.된다.정량 분석한 결과이다. 시편의 조성은 33.6 at% U, 66.4 at% O의 결과를 얻었다. 산화물 핵연료의 표면 관찰 및 정량 분석 시험시 시편 표면을 전도성 물질로 증착시키지 않고, Silver Paint 에 시편을 접착하는 방법으로도 만족한 시험 결과를 얻을 수 있었다.째, 회복기 중에 일어나는 입자들의 유입은 자기폭풍의 지속시간을 연장시키는 경향을 보이며 큰 자기폭풍일수록 현저했다. 주상에서 관측된 이러한 특성은 서브스톰 확장기 활동이 자기폭풍의 발달과 밀접한 관계가 있음을 시사한다.se that were all low in two aspects, named "the Nonsignificant group". And the issues were high risk perception in

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Effects of Lipopolysaccride-induced Stressor on the Expression of Stress-related Genes in Two Breeds of Chickens (Lipopolysaccride 감염처리가 닭의 품종간 스트레스연관 유전자 발현에 미치는 영향)

  • Jang, In Surk;Sohn, Sea Hwan;Moon, Yang Soo
    • Korean Journal of Poultry Science
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    • v.44 no.1
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    • pp.1-9
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    • 2017
  • The objective of the present study was to determine the expression of genes associated with lipopolysaccharide (LPS)-induced stressor in two breeds of chickens: the Korean native chicken (KNC) and the White Leghorn chicken (WLH). Forty chickens per breed, aged 40 weeks, were randomly allotted to the control (CON, administered the saline vehicle) and LPS-injected stress groups. Samples were collected at 0 and 48 h post-LPS injection, and total RNA was extracted from the chicken livers for RNA microarray and quantitative real-time polymerase chain reaction (qRT-PCR) analyses. In response to LPS, 1,044 and 1,193 genes were upregulated, and 1,000 and 1,072 genes were downregulated in the KNC and WLH, respectively, using a ${\geq}2$-fold cutoff change. A functional network analysis revealed that stress-related genes were downregulated in both KNC and WLH after LPS infection. The results obtained from the qRT-PCR analysis of mRNA expression of heat shock 90 (HSP90), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), activating transcription factor 4 (ATF4), sterol regulatory element-binding protein 1 (SREBP1), and X-box binding protein 1 (XBP1) were confirmed by the results of the microarray analysis. There was a significant difference in the expression of stress-associated genes between the control and LPS-injected KNC and WLH groups. The qRT-PCR analysis revealed that the stress-related $HSP90{\alpha}$ and HMGCR genes were downregulated in both LPS-injected KNC and WLH groups. However, the HSP70 and $HSP90{\beta}$ genes were upregulated only in the LPS-injected KNC group. The results suggest that the mRNA expression of stress-related genes is differentially affected by LPS stimulation, and some of the responses varied with the chicken breed. A better understanding of the LPS-induced infective stressors in chicken using the qRT-PCR and RNA microarray analyses may contribute to improving animal welfare and husbandry practices.

Interleukin-2 production and alteration of T cell subsets in mice infected with Naegleria fowleri (Naegleria fowleri 감염 마우스에 있어서 interleukin-2 생성 및 T 림프구 아형변동)

  • Yu, Cheol-Ju;Sin, Ju-Ok;Im, Gyeong-Il
    • Parasites, Hosts and Diseases
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    • v.31 no.3
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    • pp.249-258
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    • 1993
  • Naegleria fowleri is the cause of primary amoebic meningoencephalitis in man, IL-2 levels after stimulation of T lymphocytes by PHA or N.fowleri lysates. the amounts of T lymphocyte subsets and the blastogenic responses of T lymphocytes in mice after Infected with pathogenic N. fowleri were studied comparing between two study groups, one $1{\;}{\times}{\;}10^4$ trophozoites inoculated mice and the other $1{\;}{\times}{\;}10^5$ trophozoites inoculated mice. All experimental samples were obtained on the day 7, 14 and 24 after inoculation. The mice inoculated with $1{\;}{\times}{\;}10^4$ trophozoites showed a 14.3% mortality rate, and 72.2% in the mice inoculated with $1{\;}{\times}{\;}10^5$ trophozoites. The IL-2 levels on day 14 of two experimental groups were significantly decreased as compared with the control group. Thy 1.2+T cells in the total spleen Iymphocytes of $1{\;}{\times}{\;}10^5$ trophozoites inoculated group on day 7 were significantly increased compared with the control group. There was no significant difference between $1{\;}{\times}{\;}10^4$ trophozoites inoculated group and the control group. $L3T4^{+}{\;}T$ cells and $Ly2^{+}$ T cells in the total spleen Iymphocytes of $1{\;}{\times}{\;}10^5$ trophozoites inoculated group on day 7 were sigrlificantly increased compared with the control group. The DNA S fraction of T cells in the spleen of $1{\;}{\times}{\;}10^5$ trophozoites inoculated group was significantly increased on day 7. The amount of S fractions of DNA were sequentially decreased on day 14 and 24 but they were also signiacantly increased compared with the control group. The results obtained in the experiments indicats that cell mediated immunity after N.fowleri infection acts on very important host's protection immunity around the 7th day after infection. IL-2 level was much suppressed on day 14 which resulted from the exhaustion of host immune response. It was observed that the level of IL-2 production ability and the amounts of T lymphocytes subsets and the blastogenic responses of T lymphocytes were not well correlated during the observation period.

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Histological Observation of the Barbel in Common Carp, Cyprinus carpio and Bagrid Catfish, Pseudobagrus fulvidraco (잉어, Cyprinus carpio와 동자개, Pseudobagrus fulvidraco 수염의 조직학적 관찰)

  • Lim, Sang-Gu;Han, Hyoung-Kyun;Park, Hye-Jung;Park, In-Seok
    • Journal of Fisheries and Marine Sciences Education
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    • v.26 no.2
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    • pp.245-256
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    • 2014
  • 잉어, Cyprinus carpio와 동자개, Pseudobagrus fulvidraco의 상악 하악 수염을 조직학적으로 조사하였다. 동자개의 수염은 연골성 증축(axial rod of cartilage), 신경섬유다발(bundle of nerve fiber), 표피(epidermis), 평활근 층(smooth muscle layer) 및 미뢰(taste bud)로 구성되었으며, 잉어의 수염은 표피, 신경섬유다발, 혈관(blood vessel) 및 미뢰로 구성되었다. 수염 길이에서 잉어는 상악 바깥쪽 수염(second maxillary barbel)이 상악 안쪽 수염(first maxillary barbel) 보다 길게 나타났으며, 동자개는 하악 안쪽(inner mandibular barbel), 상악 위쪽(upper maxillary barbel), 하악 바깥쪽(outer mandibular barbel), 상악 아래쪽(lower maxillary barbel) 순으로 길게 나타났다(P<0.05). 미뢰의 수를 고려하였을 때, 동자개와 잉어간의 미각에 대한 유의적 차이가 없었다(P>0.05). 아울러, 두 어종의 모든 수염에서 수염 상부의 미뢰 수가 하부의 미뢰 수 보다 높게 나타났다(P<0.05). 본 연구 결과, 동자개의 수염은 딱딱하며 굴절성인 수염(flexible and stiff type)이었으며 잉어의 수염은 연하고 유연한 수염(tender and yielding type)으로 파악되었다.

Study on the Optimum Age of Physiological Reproduction in Korean Native Cattle (한우의 생리적인 최적 번식적령기에 관한 연구)

  • 성환후;이연근;최선호;장원경;이장형
    • Korean Journal of Animal Reproduction
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    • v.26 no.2
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    • pp.193-199
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    • 2002
  • This study was conducted to investigate the optimal physiologic mating time in Hanwoo for protection to decrease of reproductivity and improvement of production of offspring. We observed 32 cows that were devide into 4 parts of treatment : T1(12 months of age and 0.5kg daily gain), T2(12 months of age and 0.8kg daily gain), T3(15 months of age and 0.5kg daily gain) and T4(18 months and 0.5kg daily gain). The first heat of treated cows was 263.3$\pm$6.4 days and average weight was 181.1$\pm$11.3kg. It was revealed the conception rates of first insemination were 25%(T1), 75%(T4) and number of insemination of T3 and T4(both 1.5) was lower than T1 and T2(2.3 and 2.4). In return of estrus after heifer's first parturition, they(T1, T2, T3 and T4) showed 66.2 days, 76.7 days, 62.4 days and 68.5 days respectively and the average was 65.7 days. Plasma progesterone(P4) concentration was nearly the same during the observation periods of treated cows and P4 was released just after 12 months. Only 5 cows (15.6%) in 32 were showed normal estrus cycle and ovulation before 12 months. Before and after parturition, P4 concentration was decreased fastly and then there was no detection of P4 from after parturition to 40 days after milking. P4 would be released again on 45 day after parturition. The results were summarized as that the optimal mating time of Hanwoo heifers was decided by the 14 months of age, 110 cm height and 265kg weight.

Thinning Intensity Effects on Carbon Storage of Soil, Forest Floor and Coarse Woody Debris in Pinus densiflora Stands (간벌 강도가 소나무림의 토양, 낙엽층 및 고사목 탄소 저장량에 미치는 영향)

  • Ko, Suin;Yoon, Tae Kyung;Kim, Seongjun;Kim, Choonsig;Lee, Sang-Tae;Seo, Kyung Won;Son, Yowhan
    • Journal of Korean Society of Forest Science
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    • v.103 no.1
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    • pp.30-36
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    • 2014
  • This study examined the change in carbon (C) storage of soil, forest floor and coarse woody debris (CWD) for different thinning intensities in Pinus densiflora stands 4 years after the treatment. Two study stands were located in Jeongseon (Stand 1) and Gwangneung Experiment Forest (Stand 2). Three plots for different thinning intensities based on stand density were established at each stand in 2008; control plot (0%), T20 plot (20%) and T30 plot (30%) in Stand 1 and control plot (0%), T39 plot (39%) and T74 plot (74%) in Stand 2, respectively. The C storage of soil (0-50 cm), forest floor and CWD was measured in 2012. Total C storage of T30 plot ($109.80t{\cdot}C{\cdot}ha^{-1}$) was significantly higher than that of control plot ($86.69t{\cdot}C{\cdot}ha^{-1}$) in Stand 1. In stand 2, total C storage of T74 plot ($97.02t{\cdot}C{\cdot}ha^{-1}$) was significantly higher than that of control plot ($72.04t{\cdot}C{\cdot}ha^{-1}$) and T39 plot ($63.25t{\cdot}C{\cdot}ha^{-1}$). Total C storage of the heaviest thinned plot was the highest in each study stand. Since this study examined initial effects of thinning on C storage of soil, forest floor and CWD, further studies would be necessary to evaluate the long-term effects.

The Public Key Polynomial Cryptosystem for Data Security in Communication Networks (통신 네트워크의 정보보호를 위한 공개키 다항식 암호시스템)

  • Yang, Tae-Kyu
    • The Journal of Information Technology
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    • v.6 no.4
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    • pp.59-68
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    • 2003
  • In this paper, a public key kanpsack cryptosystem algorithm is based on the security to a difficulty of polynomial factorization in computer communication is proposed. For the proposed public key kanpsack cryptosystem, a polynomial vector B(x,y,z) is formed by transform of superincreasing vector A, a polynomial f(x,y,z) is selected. Next then, the two polynomials B(x,y,z) and f(x,y,z) is decided on the public key. Therefore a public key knapsack cryptosystem is based on the security to a difficulty of factorization of a polynomial f(x,y,z)=0 with three variables. In this paper, a public key encryption algorithm for data security of computer network is proposed. This is based on the security to a difficulty of factorization. For the proposed public key encryption, the public key generation algorithm selects two polynomials f(x,y,z) and g(x,y,z). The propriety of the proposed public key cryptosystem algorithm is verified with the computer simulation.

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Plant Community Structure of Chayang-chon Area in Soraksan National Park (설악산국립공원 자양천지역 식물군집구조)

  • 이경재;조현서;김지석
    • Korean Journal of Environment and Ecology
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    • v.11 no.4
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    • pp.433-449
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    • 1998
  • To study the plant community structure of Chayang-chon area from Jangsudae to Hangyeryung in Soraksan National Park, 62 plots(each size 100m$^2$) were set up and surveyed. Six communities(Pinus densiflora - Populus caximowiczii community, P. densiflora community, P. densiflora - Deciduous broad-leaved tree community, Quercus mongolica community, Q. mongolica - Carpinus cordata community, C. cordata - Deciduous broad- leaved tree community) were classified by DCA ordination techniques. Q. mongolica community, Q. mongolica - C. cordata and C. cordata - Deciduous broad-leaved tree community were distrivuted over altitude 755m, and the others were distributed below there. In the community over altitude 755m, the succession that Q. mongolica community changed to C. cordata community have proceeded partly and otherwise C. cordata community and Deciduous broad-leaved tree community have been competing with each other. In the community with distribytion range from 500m to 560, high, P. densiflora community would change deciduous broad-leaved tree community forward.

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Restoration and Status of Urban Ecosystem in Seoul - Plant Community Structure in Forest Area - (서울 도시생태계 현황과 회복대책(I) - 산림지역 식물군집구조 -)

  • Lee, Kyong-Jae;Cho, Woo;Han, Bong-Ho
    • Korean Journal of Environment and Ecology
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    • v.10 no.1
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    • pp.113-127
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    • 1996
  • To investigate the plant community structure and to establish restoration counterplan of forest area in Seoul, fifty six plots(each size was 400m$^{2}$) were set up and surveyed. According to analysis DCA ordination techniques, the communities were divided into six community, P. rigida community, Populus * albagladulosa community, Quercus acutissima community and Q. monholica community. The successional trend of forest area in Seoul was seem to Pinus densiflora, Robinia pseudoacacia, P. rigida, Populus * albagladulosa, Wuercus acutissima$\longrightarrow $Q. mongolixa, Q. serrata by analysis of plant community structure. Shanon's diversity was 1.1381~1.2694 and Soil pH was 3.18~4.76 in survey sites.

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