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Process development of a virally-safe dental xenograft material from porcine bones (바이러스 안전성이 보증된 돼지유래 골 이식재 제조 공정 개발)

  • Kim, Dong-Myong;Kang, Ho-Chang;Cha, Hyung-Joon;Bae, Jung Eun;Kim, In Seop
    • Korean Journal of Microbiology
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    • 제52권2호
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    • pp.140-147
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    • 2016
  • A process for manufacturing virally-safe porcine bone hydroxyapatite (HA) has been developed to serve as advanced xenograft material for dental applications. Porcine bone pieces were defatted with successive treatments of 30% hydrogen peroxide and 80% ethyl alcohol. The defatted porcine bone pieces were heat-treated in an oxygen atmosphere box furnace at $1,300^{\circ}C$ to remove collagen and organic compounds. The bone pieces were ground with a grinder and then the bone powder was sterilized by gamma irradiation. Morphological characteristics such as SEM (Scanning Electron Microscopy) and TEM (Transmission Electron Microscopy) images of the resulting porcine bone HA (THE Graft$^{(R)}$) were similar to those of a commercial bovine bone HA (Bio-Oss$^{(R)}$). In order to evaluate the efficacy of $1,300^{\circ}C$ heat treatment and gamma irradiation at a dose of 25 kGy for the inactivation of porcine viruses during the manufacture of porcine bone HA, a variety of experimental porcine viruses including transmissible gastroenteritis virus (TGEV), pseudorabies virus (PRV), porcine rotavirus (PRoV), and porcine parvovirus (PPV) were chosen. TGEV, PRV, PRoV, and PPV were completely inactivated to undetectable levels during the $1,300^{\circ}C$ heat treatment. The mean log reduction factors achieved were $${\geq_-}4.65$$ for TGEV, $${\geq_-}5.81$$ for PRV, $${\geq_-}6.28$$ for PRoV, and $${\geq_-}5.21$$ for PPV. Gamma irradiation was also very effective at inactivating the viruses. TGEV, PRV, PRoV, and PPV were completely inactivated to undetectable levels during the gamma irradiation. The mean log reduction factors achieved were $${\geq_-}4.65$$ for TGEV, $${\geq_-}5.87$$ for PRV, $${\geq_-}6.05$$ for PRoV, and $${\geq_-}4.89$$ for PPV. The cumulative log reduction factors achieved using the two different virus inactivation processes were $${\geq_-}9.30$$ for TGEV, $${\geq_-}11.68$$ for PRV, $${\geq_-}12.33$$ for PRoV, and $${\geq_-}10.10$$ for PPV. These results indicate that the manufacturing process for porcine bone HA from porcine-bone material has sufficient virus-reducing capacity to achieve a high margin of virus safety.

Changes in phytoestrogen contents and antioxidant activities during fermentation of soybean-powder milks prepared from different soybean cultivars by Lactobacillus plantarum P1201 (Lactobacillus plantarum P1201에 의한 콩 품종별 콩-분말 두유 발효 과정에서의 식물성 에스트로젠 함량과 항산화 활성의 변화)

  • Hwang, Chung Eun;Haque, Md. Azizul;Lee, Jin Hwan;Ahn, Min Ju;Lee, Hee Yul;Lee, Byong Won;Lee, Yu-Young;Lee, Choonwo;Kim, Byung Joo;Park, Ji-Yong;Sim, Eun-Yeong;Lee, Dong Hoon;Ko, Jong Min;Kim, Hyun Tae;Cho, Kye Man
    • Korean Journal of Microbiology
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    • 제52권2호
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    • pp.202-211
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    • 2016
  • This study evaluated the changes of phytoestrogen contents and antioxidant activities of soybean-powder milk (SPM) prepared from yellow soybean during fermentation with Lactobacillus plantarum P1201. In consequence, the levels of total phenolic and isoflavone-aglycone contents, ABTS and DPPH radical-scavenging activities, and FRAP assay values increased, while isoflavone-glycoside contents decreased during fermentation. The highest levels of daidzein, glycitein, and genistein were present in the Daepung SPM at concentrations of 177.92, 20.64, and $106.14{\mu}g/g$, respectively after 60 h of fermentation. Moreover, Daepung SPM showed the highest DPPH radical-scavenging activity of 48.54%, an ABTS radical-scavenging activity of 99.25%, and a FRAP assay value of 0.84 at the end of fermentation. The fermented Daepung SPM possessed highest isoflavone aglycone contents and antioxidant activities, which can be utilized for the development of functional foods.

Establishment of rhizosphere microbes for plant protection on soil-borne diseases -Benificial antagonist and its mode of action toward ginseng root rot pathogen- (근권미생물과 토양병방제 -유용길항균이 인삼근부병원에 미치는 영향-)

  • Kim, S.I.;Lee, M.W.
    • The Korean Journal of Mycology
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    • 제22권1호
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    • pp.50-61
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    • 1994
  • From soil samples, 380 antagonistic microorgnisms were isolated. Among the isolates, 42 strains had mycelia growing inhibition ability against Fusariun solani, ginseng root rot causing pathogen. Isolates CHA 1 and S-PFHR 6 were proposed as antagonists for this study and they were identified as Promicromonospora sp. and Pseudomonas pseudoalcaligenes respectively. As an antagonism against hyphae of F. solani in dual culture test, CHA 1 and S-PFHR 6 inhibited linear growing, caused abnormal branching, and the membrane projection which formed by cell wall destruction. The secondary metabolites contained in the culture filtrates which prepared from PD broth and Nutrient broth inhibited the spore germination to 14.3%. The culture filtrate of S-PFHR 6 which prepared by a little amount of soil extract addition to nutrient rich medium had more strongly. inhibited the spore germination and spore germination decreased to less than 4.0% in it. The soil used in this study had fungistasis and the germination rate of macroconidia and chlamydospore of F.solani was 19.4% and 17.7% respectively. The steam sterilized soil lost fungistasis and germination rate of conidia increased to more than 97.9%. The soils amended with the propagule of CHA 1 and S-PFHR 6 increased fungistasis and the germination rate of macroconidia decreased to 14.7% and 11.7% respectively in each treatments. But the soil ammended with glucose and asparagine annulled fungistatic ability and the germination rate of macroconidia increased to more than 48.0%. As an antagonistic activity of the secondary metabolites of two antagonistic isolates in soil, the germination rate of macroconidia of F. solani was 9.3% in the soil amended with the culture filtrate of CHA 1 but the culture filtrate of S-PFHR 6 had no such activity. In the soil which treated with antagonist propagule or culture filtrate, the chlamydospore germination rate was lower than that in natural soil. The addition of glucose and asparagine to antagonist propagule treated soil did not enhanced the chlamydospore germination.

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Floral Studies on Korean Wood-rotting Fungi (II) -on the flora of the Aphyllophorales(Basidiomycotina)- (한국산(韓國産) 목재부후균류(木材腐朽菌類)의 분포상(分布相)에 대한 연구(硏究) (II) -담자균류(擔子菌類) 민주름버섯목(目)의 분포(分布)에 대하여-)

  • Jung, Hack-Sung
    • The Korean Journal of Mycology
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    • 제22권1호
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    • pp.62-99
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    • 1994
  • The fungal flora of Korean wood-rotting fungi were studied for two years from March of 1990 to February of 1992. Fresh fungi were collected from national parks, local areas, and islands throughout the country. Fleshy fungi of the Aphyllophorales were identified through specimen examination and literature studies. Total 217 species and 1 variety were counted, among which Aleurodiscus cerussatus, Botryobasidium obtusisporum, Ceraceomyces cystidiatus, Erythricium hypnophilum, Grandinia crustosa, Grandinia spathulata, Hyphoderma praetermissum, Hyphoderma roseocremeum, Hypochnicium bombycinum, Hypochnicium detriticum, Hypochnicium lundellii, Laeticorticium roseum, Mycoacia fuscoatra, Peniophora incarnata, Phanerochaete avellanea, Phanerochaete filamentosa, Phanerochaete martelliana, Phlebia lilascens, and Trechispora vaga under the Corticiaceae, Chondrostereum purpureum and Cystostereum subabruptum under the Stereaceae, Tomentella pilosa under the Thelephoraceae, Asterostroma laxum, Hymenochaete cruenta, Hymenochaete fuliginosa, Hymenochaete tabacina, Inonotus radiatus, and Phellinus pomaceus under the Hymenochaetaceae, Antrodia crassa, Antrodia serialis, Ceriporia reticulata, Oligoporus balsameus, Oligoporus guttulatus, Oxyporus cuneatus, Rigidoporus microporus, and Trichaptum laricinum under the Polyporaceae, total 36 species were confirmed as unrecorded species to Korea and are registered here with Korean names and English descriptions.

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Flexible Specialization: A New Paradigm for Modern Industrial Society ? (柔軟的 專門化(Flexible Specialization) : 현대 産業社會의 새로운 패러다임 ?)

  • Lee, Deog-An
    • Journal of the Korean Geographical Society
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    • 제28권2호
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    • pp.148-162
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    • 1993
  • There is much speculation that modern capi-talist society is undergoing fundamental and qualitative chnge towards flexible specialization. The purpose of this study is to examine this hypothesis. This paper focusses on: the idea of flexible specialization; the significance of this transition; industrial district; and the implicati-ons of this new production system for Korean industrial space. Main arguments of this study are as follows: First, as all different groups of researchers apply the idea of flexible specialization according to their own specifications, the current debate on this topic is not much fruitful. Not surpri-singly, the concept of flexible specialization has overlapped with subocontracting. This intergration of subcontracting into flexible specialization systems, however, is inappropriate because the two concepts have different historical contexts. The other cause of this controversy is its inherent weekness, conceptual ambiguity. Thus, today's flexibility becomes tomorrow's rigidity. Secondly, transition towards flexible speciali-zation has only been partially achieved even in advanced capitalist countries. The application of dualistic explanatory framework, such as rigidity versus flexibiity, mass production versus small-lot multi-product production, and de-skilling versus re-skilling, has resulted in great exaggeration of the transformation, from Fordism to post-Fordism. There is no intermediary part between two places. Considering that the workers allocated to the Fordist mass production assembly line are not as large as one might imagine, the shift from mass to flexible production has only limited implications for the transformation of capitalist economy. Thirdly, 'industrial district' contorversy has contributed to highlighting the importance of small firms and areas as production space. The agglomeration of small firms in specific areas is common in Korea, but it is quite different from the industrial district based on flexible specialization. The Korean phenomenon stems from close interactions with its major parent firm rather than interactions between flexible, specialized, autonomous and technology-intensive smll firms. Most Korean subcontractors are still low-skilled, labour-intensive, and heavily dependent on their mojor parent firms. Thus, the assertion that the Seoul Metropolitan Area adopts flexible specialization has no base. Fourthly, the main concern of flexible speciali zation is small firms. However, the corporate organization that needs product diversification and technological specialization is oligopolistic large corporations typified by multinational corporations. It is because of this that most of these organizations are adoptiong Fordist mass production methods. The problem of product diversification will be resolved naturally if economic internationalization progresses further. What is more important for business success is the quality and price competitiveness of firms rather than product diversification. Lastly, in order to dispel further misunderst-anding on this issue, it is imparative that the conceptual ambiguity is resolved most urgently. This study recommends adoption of more speci-fied and direct terminology (such as, factory automation, computer design, out-sourcing, the exploitation of part-time labor, job redesign) rather than that of ideological ones (such as, Taylorism, Fordism, neo-Taylorism, neo-Fordism, post-fordism, flexible specialization, peripheral post-Fordism). As the debates on this topic just started, we still have long way to go until consensus is reached.

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A Prediction Model for Forecast of the Onset Date of Changmas (장마 시작일 예측 모델)

  • Lee, Hyoun-Young;Lee, Seung-Ho
    • Journal of the Korean Geographical Society
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    • 제28권2호
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    • pp.112-122
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    • 1993
  • Since more than 50${\%}$ of annual precipitation in Korea falls during Changma, the rainy season of early summer, and Late Changma, the rainy season of late summer, forcasting the onset days Changmas, and the amount related rainfalls would be necessary not only for agriculture but also for flood-control. In this study the authors attempted to build a prediction model for the forecast of the onset date of Changmas. The onset data of each Changma was derived out of daily rainfall data of 47 stations for 30 years(1961~1990) and weather maps over East Asia. Each station represent any of the 47 districts of local forecast under the Korea Meteorological Administration. The average onset dates of Changma during the period was from 21 through 26 June. The dates show a tendency to be delayed in El Ni${\~{n}}o years while they come earlier than the average in La Nina years. In 1982, the year of El Ni${\~{n}}o, the date was 9 Julu, two weeks late compared with the average. The relation of sea surface temperature(SST) over Pacific and Northern hemispheric 500mb height to the Changma onset dates was analyzed for the prediction model by polynomial regression. The onset date of Changma over Korea was correlated with SST in May(SST${_(5)}{^\circ}$C) of the district (8${^\circ}$~12${^\circ}S, 136${^\circ}~148${^\circ}W)of equatirial middle Pacific and the 500mb height in March (MB${_(3)}$"\;"m)over the district of the notrhern Hudson Bay. The relation between this two elements can be expressed by the regression: Onset=5.888SST${_5}"\;"+"\;"0.047MB${_(3)}$"\;"-251.241. This equation explains 77${\%}$ of variances at the 0.01${\%}$ singificance level. The onset dates of Late Changma come in accordance with the degeneration of the Subtro-pical High over northern Pacific. They were 18 August in average for the period showing positive correlation(r=0.71) with SST in May(SST)${_(i5)}{^\circ}$C) over district of IndiaN Ocean near west coast of Australia (24${^\circ}$~32${^\circ}$S, 104${^\circ}$~112${^\circ}$E), but negativ e with SST in May(SST${_(p5)}{^\circ}$ over district (12${^\circ}$~20${^\circ}$S,"\;"136${^\circ}$~148${^\circ}$W)of equatorial mid Pacific (r=-0.70) and with the 500mb height over district of northwestern Siberia (r=-0.62). The prediction model for Late Changma can be expressed by the regression: Onset=706.314-0.080 MB-3.972SST${_(p5)}+3.896 SST${_(i5)}, which explains 64${\%}$ of variances at the 0.01${\%}$ singificance level.

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A Study on Middle School Students' Satisfaction and Need for Clothing section of Home Economics in the Textbook (의생활 영역에 대한 중학생의 수업만족도 및 필요도에 관한 연구)

  • Kang Mi-Hyang;Oh Kyung-Wha
    • Journal of Korean Home Economics Education Association
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    • 제18권2호
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    • pp.63-77
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    • 2006
  • The Purpose of this study is to provide the basic data for the improvement or the contents or clothing curriculum in the 7th technology home economics of middle school. The standard of satisfaction of students' according to the detail domains and the standard of necessity and practical use and learners' patterns of activity task suggested in textbooks were evaluated. The ninth grade 169 boy students and 336 girl students in the national capital region were participated in this survey. According to the survey results, firstly, a dress domain got the highest relative importance(28.56%) while a clothes material domain took the lowest relative importance(8.07%) among various detail domains. Secondly, the standard of satisfaction according to each detail domain fell below the average. Generally girls' satisfaction for teaching was higher than boys'. Thirdly, a clothes material domain showed the lowest necessity for textbook contents according to detail domain and other domains showed above the average. The necessity for textbook contents appeared high for boy students rather than girl students. In addition, boy and girl students did not have interest in content relevance in textbook. Especially, they could not do well and understand experiments and practices in clothing section. Finally, The degree of utilization of the activity task ill textbooks was very low. Among various activity tasks, the learning by discovering and exploring were more utilized than cooperating learning.

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Food Group Assignment of Korean Soup & Stew for Desirable Target Pattern Draft - Representative Nutritional Value Calculation Based on Intake and Preference of Adolescent - (바람직한 식사패턴 작성을 위한 국과 찌개 음식군의 연구 - 청소년의 섭취량과 기호도 자료 활용한 대표영양가 산출 -)

  • Oh, Hae Ran;Kim, Youngnam
    • Journal of Korean Home Economics Education Association
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    • 제27권2호
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    • pp.137-147
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    • 2015
  • The target pattern is introduced for practicing a balanced dietary menu planning, but Korean soup and stew, cooked with several kinds of materials, have a problem with food group assignment in the target pattern. This study thus set out to calculate the representative nutritional values of Korean soup and stew based on intake(by 2010 Korean National Health and Nutrition Examination Survey, age group of 13~19) and preference(by select the 3 kinds of favorite soup and stew each). Total of 235 middle school students were participated by way of questionnaire, and data were analyzed. Representative energy value of vegetable soup and stew by intake were $65kca{\ell}$ and $116kca{\ell}$, respectively, which were very much different with the vegetable group representative energy value of $14kca{\ell}$ in target pattern. Representative energy value of meat fish egg legume soup and stew by intake were $149kca{\ell}$ and $211kca{\ell}$, respectively, which were very much different with the representative meat fish egg legume energy value of $94kca{\ell}$ in target pattern. As result, it is not proper to assign vegetable soup stew to vegetable food group and meat fish egg legume soup stew to meat fish egg legume food group. Representative energy values of soup and stew by preference were not much different except meat fish egg legume soup($149kca{\ell}$ by intake, $218kca{\ell}$ by preference). As conclusions, it maybe desirable to categorize soup and stew as independent food group. For more accurate energy adjustment in menu planning, devide soup and stew, and further divide to vegetable and meat fish egg legume groups may necessary.

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A Study of 'Families' as presented during the Technology-Home Economics Subject in Middle School: Focusing on the 'The Changing Family' of the 2007 Revised Curriculum (중학교 기술.가정 교과서에 나타난 '가족'에 관한 연구 - 2007 개정 교육과정의 '변화하는 가족' 단원을 중심으로 -)

  • Jun, Mi-Kyung
    • Journal of Korean Home Economics Education Association
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    • 제24권2호
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    • pp.29-49
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    • 2012
  • The purpose of this study is to look at families as presented on the technology-home economics subject of middle school. To do this, I analyzed the 2007 revised curriculum of the technology-home economics subject, 'Changing Families,' in 11 technology-home economics textbooks (8th grade). The findings are as follows. First, family-related chapters are expanded compared with the previous curriculum in the 2007 revised curriculum. In addition, the new version emphasizes understanding and acceptance of change in families to improve the family life of adolescents. Second, in the 11 types of technology-home economics textbooks, the focusing was on the meaning of the family, the family structure, the function of the family, family roles and family values. There were also no major differences in the aspect of development. Third, in the technology-home economics textbooks, the family was defined as the 'basic group of society', 'a group composed by blood relationships, marriage and adoption', 'an affective group' and 'cohabiting group'. At the same time, there were many cases in which the description of the family was overly romanticized. Such a description of the family does not match the individual family experience of an adolescent. Fourth, all of textbooks dealt with the diversity of the family structure, such as single-parent families, remarriages families, and multi-cultural families. However, the structural characteristics and problems with these types of families are excessively emphasized, which can result in students having stereotypical images of specific family types. Fifth, the explanation of the function of the family was similar among textbooks. The importance of intergenerational cooperation and gender equality was also emphasized. However, such a concept is not considered as proper in a modern society. Thus, the description of a family based on the nuclear family should be sublated. In addition, the explanation of families overall should be developed in such a way that adolescents can interpret their own family experience rather than as an enlightening declaration of the family which disregards the dynamic relationships individual families actually experience.

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The Recognition of teachers and students on clothing department education in vocational high school (전문계 고등학교 의상과 교육에 대한 교사와 학생의 인식)

  • Jang, Ja-Kyung;Shin, Hye-Won
    • Journal of Korean Home Economics Education Association
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    • 제21권4호
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    • pp.71-89
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    • 2009
  • The purpose of this paper was to suggest effective management of clothing department in vocational high schools. Bibliographic research on ten vocational high schools' clothing departments was done. Also a survey was done to figure out what students and teachers think of clothing department education. The results were as follows. There are ten clothing departments in vocational high schools countrywide. 1493 students are enrolled in the department and they are taught by 51 teachers. Each school has two to seven laboratories. Professional subject time assignment of clothing department varies from 82 to 112 hours. Students chose clothing department in order to enter the university and their satisfaction on the department was "fair." Students' satisfaction level of professional education courses was "fair" and they felt difficulties both in theory and practice. Students answered that Embroidery/Knitting courses should be closed and Fashion Coordination be opened. Students were "fairly" satisfied with facilities for practice and felt job training was necessary. Most of them wanted to enter university after high school graduation. Teachers answered professional education curriculum was "fair." They found it necessary to improve the textbooks and felt both theory and practice difficult. They wanted Embroidery/Knitting courses to be closed and Fashion CAD course to be installed. From the teachers' view, students' prospect was better to enter university than to get a job. Teachers suffered from too much teaching-unrelated workload and wanted the number of teachers to be increased. Considering the above results, vocational high schools should concentrate their efforts on making students continue to study by extending the way to enter the same department of university as department of vocational high schools. In addition, they should develop various practice programs through field practice and educational-industrial relationship to help students get jobs. Both teachers and students think that the clothing department curriculum is difficult now. Therefore, the level of the curriculum should be adjusted. Finally, it was necessary to increase the number of teachers and to carry out teachers' training to raise quality in education.

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