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Grazing Effects of Freshwater Bivalve Unio douglasiae of the North Han River on the Cyanobacterial Bloom Waters (북한강 수계에 분포하는 말조개의 남조류 섭식특성)

  • Lee, Yeon-Ju;Kim, Baik-Ho;Hwang, Soon-Jin
    • Korean Journal of Ecology and Environment
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    • v.41 no.3
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    • pp.367-373
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    • 2008
  • A freshwater bivalve (Unio douglasiae) was examined to assess the filtering rate (FR) on the cyanobacterial assemblage in a hypertrophic lake. Animal U. douglasiae used in the present study was collected using a hand-operated dredge from the North Han River (Gapyeong, Korea). The FR was measured at different feeding conditions such as feeding interval (1, 4, 7, and 24 h), mussel size (4.2$\sim$8.1 cm, n=23), prey concentration (506.7, 409.8, 327.5, 199.7 and 88.6 ${\mu}g\;L^{-1}$), and mussel density (0.5, 1.0 and 1.5 indiv. $L^{-1}$). On the applied feeding interval, the maximum FR (0.21 L $g^{-1}h^{-1}$) and minimum feces production (FP, 0.12 mg $g^{-1}h^{-1}$) were observed at 1 and 24 hr, respectively. Both weight-based FR and FP were not correlated with the mussel size, and the values lied in a limited range with some degree of variation. Likewise, no significant relations between FR and FP were observed in the mussel size. The FR values were negatively correlated with food concentration, but positively with FP. For the food concentrations, the maximum FR (0.41 L $g^{-1}h^{-1}$) and FP (0.16 mg $g^{-1}h^{-1}$) were 88.6 ${\mu}gL^{-1}$ and 327.5 ${\mu}gL^{-1}$, respectively. These results indicate that U. douglasiae collected from the North Han River, although the filtering rate were slightly less than Keum River mussel, may be applied as a strategic bio-filter to mitigate cyanobacterial bloom in eutrophic lake.

A Continuous Process for Phosphorus Recovery from Swine Slurry with Forming Struvite (양돈액비에서 Struvite 형성으로 연속적 인의 회수기술)

  • Oh I.H.;Lee J.H.;Choi B.H.;Burns R.T.
    • Journal of Animal Environmental Science
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    • v.12 no.2
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    • pp.95-100
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    • 2006
  • This study was carried out to develop a continuous process for recovering phosphorus in swine slurry. Magnesium chloride ($MgCl_2$) was used in the test as a magnesium source and the pH was regulated by adding NaOH and aerating. The results showed that the recovery rate of soluble phosphorus (SP) has increased with the molar ratios increased. In case of pH regulated with NaOH, the recovery rates of SP with molar ratio of 1:1.5 were over 95% from both farms. The removal of ammonia-nitrogen was at levels of $4{\sim}9%$. With aeration treatment, the SP recovery rate was 66% and the removal rate of ammonia-nitrogen was 15%. The treatment of NaOH to increase pH showed better SP recovery efficiency than the aeation treatment. However, in case of ammonia-nitrogen removal, the treatment of aeration showed better results than the NaOH treatment.

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Effect of Medium Composition on Phalaenopsis Micropropagation Using Lateral Buds From Flower Stalks (액아배양에 의한 호접란 대량번식시 배지조성의 영향)

  • Park, Myung-Joo;Park, Soon-Jung;Kim, Doo-Hwan
    • Horticultural Science & Technology
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    • v.16 no.1
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    • pp.42-44
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    • 1998
  • The effect of medium composition on PLB formation and multiplication and shoot regeneration was studied to establish the micropropagation system of the tropical orchid Phalaenopsis. The highest frequence of PLB formation resulted from the VW medium with 1.2 times ion concentration, 1% sucrose, 1.5g/L PVP or 2.5g/L active charcoal, apple and potato extract and 4g/L gellan gum. The highest ratio of PLB multiplication was obtained from the VW medium with 2% sucrose, apple and potato extract and cotton plate. The shoot regeneration was the most effective with the hyponex medium with 3% apple, 3% potato and 4% banana extract.

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Effects of Bisphenol A to interspecific hybrids between marine medaka Oryzias dancena and javanese medaka O. javanicus (바다송사리, Oryzias dancena와 자바송사리, O. javanicus 간 잡종에 대한 비스페놀 A의 효과)

  • Kim, Bong-Seok;Song, Ha-Yeun;Nam, Yoon-Kwon;Kim, Dong-Soo
    • Journal of fish pathology
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    • v.24 no.2
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    • pp.131-139
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    • 2011
  • Bisphenol A (BPA) is mainly used in the production of epoxy resins and polycarbonate plastics, which is a known endocrine disruptor and acutely toxic to aquatic organisms. In this study, estrogenic effect of BPA was investigated on hybrid between Oryzias dancena and O. javanicus (ODJ). ODJ were exposed to BPA of various concentrations (eg. 2.5 mg/L, 5.0 mg/L and 10.0 mg/L) for 56 days. The growth rate, abnormality and the ratio of female and male were observed in test group and control group. As a result, the growth was $14.7{\pm}2.0$ mm in total length (TL) in 2.5 mg/L, $13.7{\pm}2.5$ mm in 5.0 mg/L, $12.8{\pm}2.5$ mm in 10.0 mg/L in test group while it was $18.0{\pm}1.2$ mm in TL in control group which was not treated with bisphenol A. The result showed that the growth decreased as the concentration of BPA increased. The abnormality rate was 13.6% in control group, 65.4% in 2.5 mg/L, 81.3% in 5.0 mg/L and 98.1 % in 10.0 mg/L which showed increase in abnormality as an increase of BPA concentration. As a result of analyzing ratio of sex in the test group and control group, 6.0% was examined to be interspecific in controls, 76.9% in 2.5 mg/L and 100.0% in 5.0 mg/L and 10.0 mg/L. In conclusion, these results suggest that BPA has estrogenic effect on ODJ.

The Spread of Contrast Media in Celiac Plexus Block (복강신경총 차단시의 조영제 확산)

  • Lee, Jung-Koo;Chung, Jung-Kil;Lee, Sung-Moon
    • The Korean Journal of Pain
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    • v.7 no.2
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    • pp.211-216
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    • 1994
  • Celiac plexus block is performed to relieve intractable upper abdominal cancer pain. Generally, celiac plexus blocks have been performed under control of X-ray fluoroscopy to determine the position of the needle tip and the spread of contrast media. During the period from March 1992 to February 1994, we have performed 21 cases of neurolytic celiac plexus block to alleviate pain of intra-abdominal malignancy. We retrospectively evaluated the location of the needle tip and the spread of contrast media. P-A views of simple abdomen demonstrated the locations of the needle tip: 66.7% of the left needle tips were in upper 3/1 of L1 (6 cases) and $T_{12}-L_1$ interspace (8 cases), 50% of the right needle tips were in upper 1/3 of $L_1$, (6 cases) and $T_{12}-L_1$ interspace (4 cases). Contrast media from the right needle spread upward to middle 1/3 of $T_{10}$ (5 cases) and middle 1/3 of $T_{11}$ (5 cases), downward to middle 1/3 of $L_1$ (6 cases) and lower 1/3 of $L_1$ (3 cases). Contrast media from the left needle were spread upward to middle 1/3 of $T_{10}$ (5 cases) and evenly to other spaces, downward to middle 1/3 of $L_1$, (4 cases) and $L_1-L_2$ interspace (6 cases). We analyze the spread of contrast media according to distance from needle tip by authors score system. Contrast media of right needle spread upward 6.1 and downward 3.4, that of left needle spread upward 6.5 and downward 3.7.

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Plant Regeneration from Protoplasts of Suspension Cultured Cells in Arabidopsis thaliana (애기장대(Arabidopsis thaliana) 현탁배양세포의 원형질체로부터 식물체 재분화)

  • 김명덕;김준철;진창덕;임창진;한태진
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.2
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    • pp.125-131
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    • 2000
  • Protoplasts of Arabidopsis thaliana were easily isolated from the shoot-forming (SF) suspension-cultured cell clusters with 4 hours-shaking condition (40 rpm) on CPD enzyme solution containing 1% cellulase R-10, 0.25% pectolyase Y-23 and 0.5% driselase. Protoplasts were cultured on liquid KAO medium supplemented with 1 mg/L 2,4-D, 0.5 mg/L kinetin, 200 mg/L spermidine and 68 g/L glucose. Also, protoplasts were cultured on 0.2 $\mu$M membrane filter placed onto CP solid medium containing the suspension cells as feeder cells in the dark at $25^{\circ}C$ for 4 weeks. Protoplast-derived-SF calli were cultured on MS medium containing 0.05 mg/L IAA, 7 mg/L 2 ip and 30 g/L sucrose under the continuous illumination for four weeks. The frequency of shoot formation was about 60%. The regenerants were transferred into potting soil to grow mature plants. The regenerants formed the silques with seeds after 8 weeks of cultures.

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A Study on the Mineral Water Quality in Asia Partial Area (아시아 일부지역의 광천수 수질특성에 관한 연구)

  • Hwang, Sang-Yong
    • Journal of environmental and Sanitary engineering
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    • v.21 no.3 s.61
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    • pp.9-14
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    • 2006
  • From March 1, 2005 to August 31, 2006 mineral water wells were selected for 13 areas in Southeast Asia (Indonesia, Philippines, Thailand, Singapore) and 20 areas in Northeast Asia (Korea, North Korea, China, Japan) and the study on their mineral water quality was analyzed. - Mineral water quality was the best in Korea. Mineral spring waters in some area of China and North Korea and in the whole area of the Southeast Asia were of poor quality. - The hardness of mineral water was the low in Korea $(10{\sim}47mg/L\;as\;CaCO_3)$, Japan $(4{\sim}66mg/L\;as\; CaCO_3)$, Geumgang-san North Korea Area $(4mg/L\;as\;CaCO_3)$. Mineral spring water in Thailand, Indonesia $(1{\sim}97.5mg/L\;as\;CaCO_3)$ and in the other area $(120{\sim}1205mg/L\;as\;CaCO_3)$ were high degree of hardness. - pH value in the mineral water of Southeast Asia $(pH\;6.7{\sim}8.2)$ and Northeast Asia $(pH\;5.9{\sim}7.9)$ was up to WHO standard $(pH\;6.5{\sim}8.5)$. Fluorine of negative ion was found in 10 mineral waters: Indonesian mineral water 'ATARTN'(0.02mg/L), Thailand mineral water 'SIAM' (0.6mg/L), 'MASAFI' (0.02mg/L). Korean mineral water 'SAEMMULNARA' (1.1mg/L), 'SANSU'(0.6mg/L), 'ICIS'(0.3mg/L), 'DONGWON SAEM-MUL'(0.03mg/L), 'PYEONGCHANG' (0.6mg/L), North Korean mineral water 'KUMGANGSAN'(0.1mg/L), Japanese mineral water 'CRYSTAL GEYSER'(0.55mg/L). However Fluorine in the other 23 mineral waters were not detectable.

Synthesis of Nucleophilic Adducts of Thiols (Ⅰ). Addition of Cysteine to $\beta$-Nitrostyrene Derivatives

  • Kim, Tae-Rin;Choi, Sung-Yong
    • Bulletin of the Korean Chemical Society
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    • v.2 no.4
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    • pp.125-129
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    • 1981
  • The addition reactions of cysteine without blocking amino and carboxyl groups to substituted and unsubstituted ${\beta}$-nitro-styrene derivatives were investigated. ${\beta}$-Nitrostyrene(1a), p-methyl-${\beta}$-nitrostyrene(1b), 3,4,5-trimethoxy-$[\beta}$ -nitrostyrene(1c), $[\varpi}$-3,4-methylenedioxy-${\beta}$ -nitrostyrene(1d), o-, m- and p-chloro-${\beta}$ -nitrostyrene (1e, 1f, 1g) and o-, m- and p-methoxy-${\beta}$-nitrostyrene (1h, 1i, 1j) easily undergo addition reactions with cysteine to form S-(2-nitro-1-phenylethyl)-L-cysteine(3a), S-[2-nitro-1-(p-methyl)phenyl-ethyl]-L-cysteine(3b), S-[2-nitro-1-(3',4',5'-trimethoxy) phenylethyl]-L-cysteine(3c), S-[2-nitro-1-($[\vatpi}$ -3',4'-methylenedioxy)phenylethyl]-L-cysteine(3d), S-[2-nitro-1-(o-chloro)phenylethyl]-L-cysteine(3e), S-[2-nitro-1-(m-chloro)-phenylethyl]-L-cysteine(3f), S-[2-nitro-1-(p-chloro)phenylethyl]-L-cysteine(3g), S-[2-nitro-1-(o-methoxy)phenylethyl]-L-cysteine(3h), S-[2-nitro-1-(m-methoxy)phenylethyl]-L-cysteine(3i) and S-[2-nitro-1-(p-methoxy)phenylethyl]-L-cysteine(3j), respectively. The structure of adducts were confirmed by means of UV-spectrum, IR-spectrum, molecular weight measurement and elemental analysis. The various factors effecting the yield of cysteine adducts to ${\beta}$-nitrostyrene derivatives were also studied.

Segmental Lordosis of the Spondylolytic Vertebrae in Adolescent Lumbar Spondylolysis: Differences between Bilateral L5 and L4 Spondylolysis

  • Sugawara, Kazuhiro;Iesato, Noriyuki;Katayose, Masaki
    • Asian Spine Journal
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    • v.12 no.6
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    • pp.1037-1042
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    • 2018
  • Study Design: Retrospective study. Purpose: This study aimed to investigate whether segmental lumbar hyperlordosis of the affected vertebra in patients with spondylolysis occurs only at L5 or also occurs at L4. Overview of Literature: To the best of our knowledge, increase in segmental lordosis of the spondylolytic vertebrae has only been investigated in bilateral L5 spondylolysis; it has not been examined at different levels of bilateral spondylolysis. According to the characteristics of segmental lordosis in bilateral L5 spondylolysis, patients with bilateral L4 spondylolysis may also have increased segmental lordosis of the L4 vertebra. Methods: Patients with bilateral spondylolysis of the L5 or L4 vertebra in 2013-2015 were retrospectively identified from the hospital database. Standing lateral lumbar radiographs were assessed for the angle of segmental lordosis of the L5 and L4 vertebra, sacral slope, and lumbar lordosis. The differences in segmental lordosis of the L5 and L4 vertebra, sacral slope, and lumbar lordosis were determined using non-paired Student t-test. Results: Overall, 15 cases of bilateral L4 spondylolysis and 41 cases of bilateral L5 spondylolysis satisfied the inclusion and exclusion criteria. Lordosis of the L4 vertebra was significantly greater in the bilateral L4 spondylolysis group ($24.2^{\circ}{\pm}7.0^{\circ}$) than that in the L5 spondylolysis group ($20.3^{\circ}{\pm}6.1^{\circ}$, p=0.047). Lordosis of the L5 vertebra was significantly lower in the L4 spondylolysis group ($27.7^{\circ}{\pm}8.2^{\circ}$) than that in the L5 spondylolysis group ($32.5^{\circ}{\pm}7.3^{\circ}$, p=0.040). The sacral slope and lumbar lordosis did not significantly differ between the groups. Conclusions: Adolescent patients with bilateral spondylolysis have segmental hyperlordosis of the affected vertebra not only at the L5 level but also at the L4 level.

Occurrence of Radionuclides in Groundwater of Korea According to the Geological Condition (국내 지질별 지하수내 자연방사성물질의 산출특성)

  • Yun, Sang Woong;Lee, Jin-Yong;Park, Yu-Chul
    • The Journal of Engineering Geology
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    • v.26 no.1
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    • pp.71-78
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    • 2016
  • This study aimed to evaluate the occurrence of natural radionuclides in Korean groundwater. Groundwater radionuclide data for the period 2000-2011 were obtained from the National Institute of Environmental Research and published literature, classified into five groups according to host rock type, and used to construct detailed concentration maps. Radon, uranium, gross-α, and radium concentrations ranged from 0.4 to 64,688.0 pCi/L (mean: 4,907 pCi/L), 0 to 2,297 μg/L (mean: 27.5 μg/L), 0 to 312 pCi/L (mean: 3.9 pCi/L), and 0 to 17.4 pCi/L (mean: 0.2 pCi/L), respectively. Radon concentrations in 562 of a total 1,501 wells (i.e., 53.5%) exceeded 4,000 pCi/L, which is the acceptable contamination threshold established by the United States Environmental Protection Agency. Uranium, gross-α, and radium concentrations exceeded the respective thresholds of 30 μg/L, 15 pCi/L, and 5 pCi/L in 121 of 1,031 wells (11.9%), 34 of 978 wells (3.5%), and 4 of 89 wells (4.5%), respectively. The mean radionuclide concentration in groundwaters hosted by igneous and metamorphic rocks was higher than that in groundwaters hosted by other rock types, such as volcanics, carbonates, and other sedimentary rocks. The correlations between individual radionuclides were weak or insignificant.